{"title":"膳食中加入鳄椒和鳄椒种子对东莨菪碱致失忆症大鼠的神经保护作用。","authors":"Odunayo M Agunloye, Esther A Olawuyi, Ganiyu Oboh","doi":"10.1515/jcim-2024-0450","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>Neurodegeneration continues to drive conditions of progressive cognitive decline from amnesia to severe dementia in different age groups. Despite, the availability of standard drugs, the need for multi-targeted interventions with no side-effects remains to be explored. Hence, this study evaluates the neuroprotective effects of <i>Aframomum melegueta</i> and <i>Aframomum danielli</i> seeds dietary inclusion on neurocognitive decline in scopolamine-administered male rats.</p><p><strong>Methods: </strong>Seven groups of experimental rats were studied for 14 days: normal rats (group 1), untreated scopolamine-administered rats (group 2), scopolamine-administered rats treated with donepezil (group 3), scopolamine-administered rats treated with dietary inclusions of 4 and 8 % <i>A. melegueta</i> (groups 4 and 5 respectively), and 4 and 8 % <i>A. danielli</i> (groups 6 and 7 respectively). Rats were trained with Morris water maze, pre-treated with the diets, and administered (I. P) scopolamine (3 mg/kg body weight from days 12-14). Memory functionality was evaluated using the maze test and hippocampal and cortical brain regions were analyzed biochemically.</p><p><strong>Results: </strong>Scopolamine administration impaired working memory, evidenced by increased escape latency and activities of neurodegenerative biomarkers in untreated rats. <i>A. melegueta</i> and <i>A. danielli</i> dietary inclusions significantly reduced escape latency, improved working memory, and restored key biomarkers, including acetylcholinesterase, butyrylcholinesterase, monoamine oxidase, and reactive oxygen species. Treated rats also exhibited elevated total thiol levels, and improved anti-inflammatory markers (IL-10).</p><p><strong>Conclusions: </strong>Both <i>A. melegueta</i> and <i>A. danielli</i> seeds showed improved neuroprotective effect in the brain of scopolamine-induced amnesia rats highlighting significant intervention against cognition. However, <i>A. melegueta</i> showed greater neuroprotective efficacy.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Neuroprotective effects of dietary inclusions of alligator pepper (<i>Aframomum melegueta</i>) and bastard melegueta (<i>Aframomum danielli</i>) seeds on scopolamine-induced amnesia in rats.\",\"authors\":\"Odunayo M Agunloye, Esther A Olawuyi, Ganiyu Oboh\",\"doi\":\"10.1515/jcim-2024-0450\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>Neurodegeneration continues to drive conditions of progressive cognitive decline from amnesia to severe dementia in different age groups. Despite, the availability of standard drugs, the need for multi-targeted interventions with no side-effects remains to be explored. Hence, this study evaluates the neuroprotective effects of <i>Aframomum melegueta</i> and <i>Aframomum danielli</i> seeds dietary inclusion on neurocognitive decline in scopolamine-administered male rats.</p><p><strong>Methods: </strong>Seven groups of experimental rats were studied for 14 days: normal rats (group 1), untreated scopolamine-administered rats (group 2), scopolamine-administered rats treated with donepezil (group 3), scopolamine-administered rats treated with dietary inclusions of 4 and 8 % <i>A. melegueta</i> (groups 4 and 5 respectively), and 4 and 8 % <i>A. danielli</i> (groups 6 and 7 respectively). Rats were trained with Morris water maze, pre-treated with the diets, and administered (I. P) scopolamine (3 mg/kg body weight from days 12-14). Memory functionality was evaluated using the maze test and hippocampal and cortical brain regions were analyzed biochemically.</p><p><strong>Results: </strong>Scopolamine administration impaired working memory, evidenced by increased escape latency and activities of neurodegenerative biomarkers in untreated rats. <i>A. melegueta</i> and <i>A. danielli</i> dietary inclusions significantly reduced escape latency, improved working memory, and restored key biomarkers, including acetylcholinesterase, butyrylcholinesterase, monoamine oxidase, and reactive oxygen species. Treated rats also exhibited elevated total thiol levels, and improved anti-inflammatory markers (IL-10).</p><p><strong>Conclusions: </strong>Both <i>A. melegueta</i> and <i>A. danielli</i> seeds showed improved neuroprotective effect in the brain of scopolamine-induced amnesia rats highlighting significant intervention against cognition. However, <i>A. melegueta</i> showed greater neuroprotective efficacy.</p>\",\"PeriodicalId\":15556,\"journal\":{\"name\":\"Journal of Complementary and Integrative Medicine\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-07-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Complementary and Integrative Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1515/jcim-2024-0450\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Complementary and Integrative Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/jcim-2024-0450","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
摘要
目的：在不同年龄组中，神经退行性变继续驱动从健忘症到严重痴呆的进行性认知衰退。尽管有标准药物可用，但对无副作用的多目标干预措施的需求仍有待探索。因此，本研究对东莨菪碱给药雄性大鼠的神经认知能力下降进行了研究。方法:七组大鼠的实验研究了14天:正常大鼠(组1),未经处理的scopolamine-administered老鼠(组2),scopolamine-administered老鼠多奈哌齐(组3)处理,scopolamine-administered老鼠对待饮食的夹杂物4和8 % a melegueta(组分别为4和5)和4和8 % a danielli(分别组6和7)。采用Morris水迷宫训练大鼠，并在第12-14天给予东莨菪碱（3 mg/kg体重）预处理。使用迷宫测试评估记忆功能，并对海马和大脑皮层区域进行生化分析。结果：东莨菪碱使大鼠的工作记忆受损，未治疗的大鼠的逃避潜伏期和神经退行性生物标志物的活动增加。melegueta和A. danielli饮食内含物显著降低了逃避潜伏期，改善了工作记忆，并恢复了关键生物标志物，包括乙酰胆碱酯酶、丁基胆碱酯酶、单胺氧化酶和活性氧。治疗大鼠还表现出总硫醇水平升高，抗炎标志物（IL-10）改善。结论：东莨菪碱诱导的健忘症大鼠脑内神经保护作用明显增强，对认知功能有显著的干预作用。而麻瓜则表现出更强的神经保护作用。
Neuroprotective effects of dietary inclusions of alligator pepper (Aframomum melegueta) and bastard melegueta (Aframomum danielli) seeds on scopolamine-induced amnesia in rats.
Objectives: Neurodegeneration continues to drive conditions of progressive cognitive decline from amnesia to severe dementia in different age groups. Despite, the availability of standard drugs, the need for multi-targeted interventions with no side-effects remains to be explored. Hence, this study evaluates the neuroprotective effects of Aframomum melegueta and Aframomum danielli seeds dietary inclusion on neurocognitive decline in scopolamine-administered male rats.
Methods: Seven groups of experimental rats were studied for 14 days: normal rats (group 1), untreated scopolamine-administered rats (group 2), scopolamine-administered rats treated with donepezil (group 3), scopolamine-administered rats treated with dietary inclusions of 4 and 8 % A. melegueta (groups 4 and 5 respectively), and 4 and 8 % A. danielli (groups 6 and 7 respectively). Rats were trained with Morris water maze, pre-treated with the diets, and administered (I. P) scopolamine (3 mg/kg body weight from days 12-14). Memory functionality was evaluated using the maze test and hippocampal and cortical brain regions were analyzed biochemically.
Results: Scopolamine administration impaired working memory, evidenced by increased escape latency and activities of neurodegenerative biomarkers in untreated rats. A. melegueta and A. danielli dietary inclusions significantly reduced escape latency, improved working memory, and restored key biomarkers, including acetylcholinesterase, butyrylcholinesterase, monoamine oxidase, and reactive oxygen species. Treated rats also exhibited elevated total thiol levels, and improved anti-inflammatory markers (IL-10).
Conclusions: Both A. melegueta and A. danielli seeds showed improved neuroprotective effect in the brain of scopolamine-induced amnesia rats highlighting significant intervention against cognition. However, A. melegueta showed greater neuroprotective efficacy.
期刊介绍：
Journal of Complementary and Integrative Medicine (JCIM) focuses on evidence concerning the efficacy and safety of complementary medical (CM) whole systems, practices, interventions and natural health products, including herbal and traditional medicines. The journal is edited by Ed Lui of the University of Western Ontario. Topics: -Quality, efficacy, and safety of natural health products, dietary supplements, traditional medicines and their synthetic duplicates -Efficacy and safety of complementary therapies -Evidence-based medicine and practice, including evidence of traditional use -Curriculum development, educational system and competency of complementary health programs -Methodologies on research and evaluation of traditional medicines and herbal products -Integrative medicine: basic and clinical research and practice -Innovation in CAM Curriculum -Educational Material Design
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