Journal of Complementary and Integrative Medicine最新文献

{"title":"Decoding the neuroprotective secrets of <i>Tinospora sinensis</i>: navigating the rotenone induced storm of behavioral and biochemical challenges in Parkinson's disease rat models.","authors":"Prabha Bhong, Suvarna Ingale, Pallavi Jadhav","doi":"10.1515/jcim-2026-0074","DOIUrl":"https://doi.org/10.1515/jcim-2026-0074","url":null,"abstract":"<p><strong>Objectives: </strong>Parkinson's disease (PD) is a chronic neurodegenerative disorder that leads to motor and non-motor challenges, significantly impacting the quality of life. Research highlights body's antioxidant systems role in mitigating PD. This study investigated neuroprotective capabilities of <i>Tinospora sinensis</i> stem extract (TSSE) in a rat model of PD induced by rotenone.</p><p><strong>Methods: </strong>PD was induced in rats by subcutaneous rotenone injections (2 mg/kg/day) for 35 days. TSSE was administered orally at 100, 200, and 400 mg/kg doses, with levodopa-carbidopa as the standard reference. Motor impairments were assessed through catalepsy, locomotor activity, and rotarod tests. Biochemical evaluations included oxidative stress markers, antioxidant enzymes (glutathione, superoxide dismutase, and catalase), and dopamine levels, and monoamino oxidase-B (MAO-B). Histopathological examination of brain assessed neuronal integrity.</p><p><strong>Results: </strong>Rotenone administration led to significant motor impairments, increased oxidative stress, reduced dopamine levels, and neuronal damage. Treatment with TSSE resulted in notable, dose-dependent enhancements in motor abilities and restored antioxidant enzyme activities, while also reducing lipid peroxidation, as indicated by decreased malondialdehyde levels. Additionally, TSSE decreased MAO-B level and increased brain dopamine level and maintained neuronal structure. The TSSE 400 mg/kg exhibited neuroprotective effects comparable to those of standard.</p><p><strong>Conclusions: </strong>TSSE shows significant neuroprotective effects, likely by enhancing endogenous antioxidant defenses and restoring dopaminergic neurotransmission. These findings suggest, TSSE could serve as a complementary therapeutic approach that targets oxidative and neurodegenerative mechanisms in PD.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148239186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of monk fruit sweetener on liver architecture, oxidative stress, and enzyme markers in adult Wistar rats.","authors":"Ibrahim Yusuf, Sirajo M Umar, Rabiu I Fage","doi":"10.1515/jcim-2026-0007","DOIUrl":"https://doi.org/10.1515/jcim-2026-0007","url":null,"abstract":"<p><strong>Background: </strong>Non-nutritive sweeteners (NNS) such as monk fruit sweetener (MFS) are increasingly adopted as sugar alternatives due to their high sweetening potency and low caloric content. Despite their widespread use, the metabolic and hepatic safety profiles of these agents remain underexplored. This study investigates the effect of MFS on the liver histology and key biochemical markers; alanine aminotransferase (ALT), aspartate aminotransferase (AST), and malondialdehyde (MDA) in adult male adult Wistar rats.</p><p><strong>Methods: </strong>Twenty adult male Wistar rats were assigned into four groups (n=5/group). Group A served as the control, while Groups B, C, and D received 100, 150, and 200 mg/kg body weight of MFS orally for four weeks. Liver histology was studied using H&E and Masson trichrome stained sections of the liver. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and malondialdehyde (MDA) were assessed. Data obtained was analyzed using one way ANOVA.</p><p><strong>Results: </strong>MFS administration resulted in significant elevations in ALT (p=0.001) and MDA (p=0.002), consistent with hepatocellular injury and increased oxidative stress. Although AST levels were elevated, the change did not reach statistical significance. Histological evaluation demonstrated dose-dependent hepatic alterations, including hepatocellular vacuolation, necrosis, sinusoidal and central vein congestion, and increased collagen deposition.</p><p><strong>Conclusions: </strong>Exposure to MFS was associated with biochemical and structural hepatic alterations in a dose-dependent manner, raising concerns regarding its hepatic safety under conditions of sustained use. Further mechanistic and long-term studies are warranted to clarify the translational relevance of these findings in humans.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148163310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The modulatory role of thymoquinone in boldenone undecylenate induced testicular tissue damage: oxidative stress, inflammatory markers and androgen receptor dysregulation expression in adult male rats.","authors":"Noha A Mowaad, Marwa El-Sayed El-Shamarka, Eman Refaat Youness, Eman Gomaa, Noha S E Bayomi","doi":"10.1515/jcim-2025-0378","DOIUrl":"https://doi.org/10.1515/jcim-2025-0378","url":null,"abstract":"<p><strong>Objectives: </strong>Boldenone undecylenate (Bold), a synthetic anabolic steroid, poses health risks including testicular damage. Thymoquinone (TQ), from <i>Nigella sativa</i>, has antioxidant and anti-inflammatory properties. To evaluate TQ's protective effects against Bold-induced testicular impairment.</p><p><strong>Methods: </strong>Thirty-two male Wistar rats were divided into four groups (control, TQ 25 mg/kg orally, Bold 5 mg/kg intramuscularly, and Bold + TQ) for 8 weeks. Serum FSH, LH, and free testosterone, and testicular IL-1β, MCP-1, TNF-α, MDA, and NO were measured. Testes were examined histologically and for AR expression.</p><p><strong>Results: </strong>Bold decreased FSH, LH, and testosterone, increased oxidative stress and inflammatory markers, caused histological damage, and reduced AR expression. TQ co-treatment significantly mitigated these effects.</p><p><strong>Conclusions: </strong>TQ protects against Bold-induced testicular injury by reducing oxidative stress and inflammation, suggesting its potential as a preventive supplement.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148138287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the anticancer potential of black maitake extract in A375 human malignant melanoma cells.","authors":"Joshua Mathews, Sona Regonda, Anent Grewal, Hannah Drew, Siyaram Pandey","doi":"10.1515/jcim-2025-0267","DOIUrl":"https://doi.org/10.1515/jcim-2025-0267","url":null,"abstract":"<p><p>Melanoma is one of the deadliest skin cancers, characterized by high metastatic potential and poor prognosis in advanced stages. While chemotherapeutic agent temozolomide (TMZ) is the standard treatment, its clinical utility is hindered by toxicity and limited specificity.</p><p><strong>Objectives: </strong>This study evaluates Black Maitake Odaira Extract Prothera (BME) from the <i>Grifola frondosa</i> mushroom as a complementary treatment for melanoma, examining its apoptotic, anti-migratory, and anti-proliferative effects in 2D and 3D models. We aim to investigate the therapeutic potential of BME alone and in combination with standard chemotherapy in A375 melanoma cells.</p><p><strong>Methods: </strong>A375 melanoma cells were treated with BME alone and in combination with TMZ. Cell viability was quantified via Trypan Blue exclusion assays. Apoptosis was assessed using Hoechst 33342, Annexin V, and propidium iodide staining, while mitochondrial depolarization was evaluated using TMRM staining. Cancer cell migration was analyzed through wound healing assays, and 3D spheroid cultures were used to evaluate how BME disrupts complex tumour-like structures.</p><p><strong>Results: </strong>BME induced dose-dependent apoptosis in 2D cell models. BME enhanced the efficacy of TMZ in inducing apoptosis when treated in combination. Wound healing assays revealed a marked reduction in cell migration, particularly in combination treatments with TMZ. In 3D cell cultures, BME with TMZ exhibited increased mitochondrial depolarization.</p><p><strong>Conclusions: </strong>BME demonstrates pro-apoptotic and anti-migratory activity in melanoma cells, while improving the efficacy of standard chemotherapy. These findings support its potential as an effective adjuvant natural health product for melanoma treatment.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148031380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of taurine on the cardiotoxicity induced by bisphenol-A in male albino rats.","authors":"Yasser A Khadrawy, Eman N Hosny, Noha M Khoder, Neveen A Noor, Mohamed M Nazmy, Amel I Othman, Heba S Aboul Ezz, Nasr M Radwan","doi":"10.1515/jcim-2025-0446","DOIUrl":"10.1515/jcim-2025-0446","url":null,"abstract":"<p><strong>Objectives: </strong>Due to the widespread and extensive use of the endocrine disruptor bisphenol A (BPA) in our daily life, several studies were performed to attenuate its adverse effects. Thus, taurine was used in the current study to evaluate its protective action against BPA cardiotoxic effects.</p><p><strong>Methods: </strong>Rats were divided into control rats, BPA-intoxicated rats, and BPA-intoxicated rats treated with taurine. After six weeks of daily oral BPA treatment (25 mg/kg), the cardio-protective effect of taurine (100 mg/kg) was determined by the measurement of the serum activities of lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB) and the levels of Wnt, β-catenin, lipid peroxidation (MDA), nitric oxide (NO), reduced glutathione (GSH) and Na⁺, K⁺, ATPase activity in the myocardium. Additionally, the effects of taurine on the cardiac histopathological alterations caused by BPA were evaluated.</p><p><strong>Results: </strong>BPA-treated animals exhibited significantly elevated levels of MDA (4.7 ± 0.4 vs. 3.2 ± 0.2), Wnt (4.3 ± 0.3 vs. 2.9 ± 0.1), β-catenin (781.4 ± 37.9 vs. 599.1 ± 43.2), and increased serum activities of LDH (957.9 ± 78.6 vs. 662.3 ± 30.2) and CK-MB (347.3 ± 32.1 vs. 74.9 ± 15.2). In addition, BPA significantly decreased myocardial levels of GSH (4.6 ± 0.2 vs. 5.7 ± 0.2) and NO (0.2 ± 0.01 vs. 0.2 ± 0.01) levels, and Na⁺, K⁺-ATPase (1.2 ± 0.1 vs. 1.5 ± 0.1) activity. Histopathological examination showed myocardial striations, pyknosis, congestion and dilation of blood vessels. Taurine protection alleviates BPA-induced changes in MDA, NO, GSH, Na⁺, K⁺, ATPase, LDH, CK-MB, and Wnt restoring them to control-like values. However, β-catenin level was increased in taurine-rats. Taurine also improved the histopathological changes caused by BPA. Accordingly, taurine could potentially impose cardio-protection against BPA cardiotoxicity in male rats.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147941644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Compendium of Reporting Guidelines for Traditional, Complementary, and Integrative Medicine from the Enhancing the QUAlity and Transparency Of health Research (EQUATOR) Network.","authors":"Jeremy Y Ng, Niveen Syed, David Moher","doi":"10.1515/jcim-2026-0073","DOIUrl":"10.1515/jcim-2026-0073","url":null,"abstract":"<p><p>High-quality and transparent reporting is fundamental to the credibility, reproducibility, and impact of health research. While widely endorsed reporting guidelines such as CONSORT and PRISMA have improved reporting across many fields, traditional, complementary, and integrative medicine (TCIM) research presents distinctive conceptual, methodological, and contextual challenges that are not always adequately addressed by general frameworks. In response, a growing number of TCIM-specific reporting guidelines have been developed over the past two decades. This manuscript provides a structured compendium of all 30 reporting guidelines listed on the Enhancing the QUAlity and Transparency Of health Research (EQUATOR) Network under the clinical area labelled \"Complementary and Alternative Medicine\" as of March 2026. The compendium summarizes each reporting guideline's scope, study design focus, TCIM modality, relationship to established parent reporting guidelines where applicable, and methodological strengths and weaknesses. The identified reporting guidelines span a wide range of research domains, including randomized trials, protocols, systematic reviews, case reports, case series, clinical practice guidelines, and basic research, and encompass modalities such as acupuncture, herbal medicine, homeopathy, massage, moxibustion, cupping, biofield therapies, and reflexology. By consolidating these resources in a single, accessible overview, this compendium aims to support researchers, journal editors, and peer reviewers in identifying and applying appropriate TCIM-specific reporting guidance, thereby strengthening the transparency, consistency, and overall quality of TCIM research.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":"206-227"},"PeriodicalIF":0.0,"publicationDate":"2026-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147874266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical profiling and cytotoxic potential of hydroalcoholic extract of <i>Begonia malabarica</i> in HepG2 cells: an <i>in vitro</i> phytopharmacological study.","authors":"Swaliha Chediyankandy, Akila Srinivasan, Barathane Datchanamurthy, Kannan Manivel","doi":"10.1515/jcim-2025-0365","DOIUrl":"https://doi.org/10.1515/jcim-2025-0365","url":null,"abstract":"<p><strong>Objectives: </strong>In this study, the hydroalcoholic extract of entire <i>Begonia malabarica</i> plant was screened to identify its specific phytochemical groups and <i>in vitro</i> cytotoxic potential against HepG2 (Human Hepatocellular carcinoma) cell lines.</p><p><strong>Methods: </strong>Annexin V/PI Apoptotic assay and the MTT assay, were employed in this study. MTT assay was used to evaluate <i>in vitro</i> cytotoxic potential of <i>B. malabarica</i> extract against the HepG2 cell line. The Annexin V/PI Apoptotic assay was performed to estimate the proportion of cells undergoing apoptosis. The results were compared with doxorubicin as standard.</p><p><strong>Results: </strong>The MTT assay found that at highest concentration of 500 μg/mL, the hydroalcoholic extract of <i>B. malabarica</i> reduced cell viability to 24.17 %, while doxorubicin treated cell showed cell viability to 4.8 % at highest concentration of 100 μg/mL. Flow cytometry based Annexin V analysis revealed that <i>B. malabarica</i> extract induced pronounced apoptosis in HepG2 cells, marked by a dominant late apoptotic population (70.56 %) and dead cells (22.17 %), with minimal early apoptotic (0.04 %) and viable cells (7.23 %). The percentage of cells undergone late apoptosis was identical to standard drug doxorubicin (71.2 %). These results suggest a late apoptotic response, potentially involving mitochondrial disruption, oxidative stress, or caspase activation.</p><p><strong>Conclusions: </strong>These findings highlight the presence of bioactive compounds in <i>B. malabarica</i> that can modulate cell death pathways, supporting its potential as a potential anti-cancer agent. However, more research can help in better understanding of the role of this plant as a potential new source of cancer therapy and uncover previously unknown pharmacological activities and properties.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147874329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethics in the age of artificial intelligence: reflections from mapping the terrain and implications for traditional, complementary, and integrative medicine.","authors":"Jeremy Y Ng","doi":"10.1515/jcim-2026-0082","DOIUrl":"10.1515/jcim-2026-0082","url":null,"abstract":"","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":"201-205"},"PeriodicalIF":0.0,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147773019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chenopodium ambrosioides Linn. induces mitochondrial-dependent cell death via activation of mPT pore opening, caspases and apoptotic machinery.","authors":"Adeola Oluwakemi Olowofolahan, Mimidoo Gaaba, Fatima Bulus Musa","doi":"10.1515/jcim-2025-0312","DOIUrl":"https://doi.org/10.1515/jcim-2025-0312","url":null,"abstract":"<p><strong>Objectives: </strong>Mitochondrion is an organelle involved in cellular energy generation and cell death. The mitochondrial permeability transition (mPT) pore plays a crucial role in the execution of mitochondrial-dependent cell death. Several medicinal plants evoke their therapeutic potential against tumor and cancer by instigating mitochondrial-dependent cell death. This research consequently explored the effect of chloroform fraction of <i>Chenopodium ambrosioides</i> (CFCA) on mitochondrial-dependent cell death via mPT pore.</p><p><strong>Methodology: </strong>Rats were equally assigned into four groups; A (control), B (50), C (100) and D (200) mg/kg CFCA. Mitochondria were isolated by differential centrifugation. The mPT pore opening, mitochondrial ATPase (mATPase) activity and mitochondrial-lipid peroxidation (mLPO) were assessed spectrophotometrically. Caspases 9 and 3 levels, hepatic DNA fragmentation, histology and immunohistochemical analysis of Bax, Cytochrome <i>c</i>, P53 and anti-apoptotic Bcl-2 proteins were determined. Gas Chromatography-Mass Spectrometry (GC-MS) analysis of CFCA was carried out.</p><p><strong>Results: </strong>The CFCA dose-dependently induced mPT pore opening, enhanced mATPase activity, reduced malondialdehyde generation, increased Caspases 9 and 3 levels and effected hepatic DNA fragmentation. The histological assessment showed mild to moderate steatosis and infiltration of the hepatocytes. The CFCA upregulated the expression of Bax, Cytochrome <i>c</i> and P53 proteins while anti-apoptotic Bcl-2 protein was downregulated. The GC-MS analysis revealed the presence of certain pharmacologically reactive compounds including Tridecanoic acid, 12-methyl ester, Hexadecanoic acid methyl ester, 11-Octadecadienoic acid, methyl ester, Pyrido[2,3-d]pyrimidine and 6-Undecyl-5,6-dihydro-2H-pyran-2-one.</p><p><strong>Conclusions: </strong>This study suggests the presence of phytochemicals in CFCA that could execute mitochondrial-mediated apoptosis via induction of mPT pore opening, caspases and apoptotic machinery.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147674068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antidepressant-like effect of <i>Peperomia pellucida</i> (L.) Kunth extract: analysis of behavioral and oxidative stress parameters in mice exposed to chronic unpredictable mild stress.","authors":"Fitrya Fitrya, Ghina Desfitriyana, Nyiayu Fauziyah Kurniawati, Vitri Agustiarini, Annisa Amriani, Rennie Puspa Novita","doi":"10.1515/jcim-2025-0391","DOIUrl":"https://doi.org/10.1515/jcim-2025-0391","url":null,"abstract":"<p><strong>Objectives: </strong><i>Peperomia pellucida</i> (Indonesian name: Suruhan) is a tropical plant easily found throughout Indonesia. This plant is used as a food ingredient and to treat various diseases due to its phytoconstituent content. This study aimed to evaluate the antidepressant effect of the ethanol extract of the Suruhan herb.</p><p><strong>Methods: </strong>Mice were exposed to chronic mild stress using the chronic unpredictable mild stress (CUMS) method for 21 days, followed by a sucrose preference test. The antidepressant effect was assessed by behavioral changes in the tail suspension test (TST), forced swimming test <b></b> (FST), and locomotor tests. Oxidative stress levels were measured using malondialdehyde (MDA) and catalase levels, and the evaluation was complemented by histopathological analysis. The antidepressant effect of the Suruhan extract (ES) was compared with that of normal controls, stress controls, and fluoxetine controls.</p><p><strong>Results: </strong>In the behavioral test, there was no significant difference in immobility time and number of line crossings in the test group compared to the fluoxetine control (p>0.05). ES significantly increased the sucrose preference value of the test group. Oxidative stress parameter analysis showed that the extract reduced MDA levels in mice, no different from the fluoxetine control group (p>0.05), and increased catalase levels, but not as effectively as the standard drug (p<0.05). Histopathological analysis found a significant increase in the thickness of the granular layer in the dentate gyrus of mice treated with ES and fluoxetine compared to the stress group.</p><p><strong>Conclusions: </strong>Suruhan herb has antidepressant-like effects and has the potential to be used as a functional food and complementary medicine in the treatment of depression.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147654379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}