Journal of Cytology and Histology最新文献

{"title":"Human Genetics Meet 2019: Massively parallel next generation sequencing to investigate the cis-acting genetic modifiers of somatic instability in Huntingtons disease- Asma M Alshammari- Kuwait Medical Genetic Centre","authors":"A. Alshammari","doi":"10.37421/2157-7099.2020.11.556","DOIUrl":"https://doi.org/10.37421/2157-7099.2020.11.556","url":null,"abstract":"Huntington Disease (HD) is a very factor acquired neurodegenerative issue brought about by development of a precarious CAG trinucleotide rehash in the huntingtin quality (HTT). Substantial shakiness in HD shows an age-subordinate, extension one-sided and tissue-explicit example and the most significant level of physical precariousness is found in tissues that are generally defenseless to the ailment pathology. In this way, the point of this venture was to test the speculation that substantial insecurity of the HD CAG rehash assumes a significant job in illness pathology by evaluating physical flimsiness in the quantity of CAG rehashes by cutting edge sequencing (NGS) innovation in buccal cell DNA. We built up a high-throughput sequencing pipeline to arrangement and genotype HTT alleles from blood and buccal swab DNA of the Scottish and Venezuelan populaces, individually. A sum of 210 people from the Scottish all inclusive community and 742 HD patients and unaffected people from the Venezuelan HD associate were sequenced on the MiSeq stage. We set up that it was conceivable to grouping and genotype the CAG rehashes, the polymorphic CCG rehash and the flanking arrangements. Our information feature the utility of NGS innovation as a way to deal with genotype HTT alleles, distinguish succession variations and measure physical insecurity of the CAG rehash. Our information stress that the physical precariousness in HD is age-ward and development one-sided, likewise could be a central point in ailment movement and could be an expected helpful objective in HD. We likewise researched the likelihood that there are executing modifier factors associated with deciding the level of substantial shakiness in HD patients. We genotyped polymorphisms in applicant bungle fix (MMR) qualities and inspected their impact, assuming any, on the leftover variety of substantial unsteadiness. People conveying the minor allele of rs3512 in FAN1 have a more significant level of substantial shakiness than normal, proposing that a portion of the varieties in HD physical insecurity could be represented by hereditary variety in the DNA confuse fix pathway. The quest for modifier qualities may have outcomes in understanding the obsessive procedure in HD, and may in this way give helpful focuses to future examinations. Keywords: next-generation sequencing, whole-exome-sequencing, whole-genome-sequencing, gene-panel, inherited cancer syndrome, cancer somatic mutation, diagnostics, genetic modifiers, theranostics Introduction: Cutting edge sequencing (NGS), additionally called enormous equal sequencing, was created in the most recent decade and permits concurrent sequencing of a huge number of DNA parts without past arrangement information. This trend setting innovation has been a genuine insurgency contrasted and the conventional sequencing strategies, in which one or a couple of moderately short parts of DNA, recently intensified by Polymerase Chain Reaction (PCR), could be","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"1 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88214278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraoperative Squash Cytology in CNS Lesions - How Fine is the Resolution?","authors":"Moulima Banerjee, A. Chowdhury, Paramita Paul","doi":"10.37421/2157-7099.2020.11.554","DOIUrl":"https://doi.org/10.37421/2157-7099.2020.11.554","url":null,"abstract":"Introduction: With the advent of minimally invasive/ stereotactic neurosurgical techniques accurate localization of tumor and differentiation from surrounding normal brain parenchyma has become imperative to give maximal benefit of surgery and reducing collateral neurodeficit. Despite the advancement of non-invasive radiological tools, direct evidence from the microscope during the operation guides the surgeon and gives the patient optimal benefit of the surgery. The aim of this study is to correlate intraoperative squash cytology and final histopathological diagnosis and find out efficacy and reliability of squash as a diagnostic tool. Method/study design: A cross sectional observational study conducted in the Department of Neuropathology from August 2018 to June 2019. All neurosurgical cases received for both intra-operative diagnosis and histopathology sample were included. Results: Total ninety four cases of squash smear and corresponding histopathology with immunohistochemistry was studied and overall sensitivity of squash was calculated to be 91.3%. Individual sensitivity, specificity and positive predictive value for major lesions were also calculated. Conclusion: The role of intraoperative diagnosis in CNS tumors need not be overemphasized. It can achieve targeting of lesion and provide guidance to the neurosurgeon in modifying and monitoring the surgical approach.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"5 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90613544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of Secondary Metabolites Derived from Aspergillus Species as Anticancer Agents and Related Histological and Genetic Alterations: In Vitro Study","authors":"M. Alfaifi, Tamer M. M. Abu-Amara, M. Amer, M. Nasr, Wagih M. Abdelhay, Laila E EIMoselhy, Tamer Albasyoni Gomah, S. E. Elbehairi, Harbi A. Ali, A. Mohamed","doi":"10.37421/JCH.2020.11.552","DOIUrl":"https://doi.org/10.37421/JCH.2020.11.552","url":null,"abstract":"The present work aimed to use natural derived fungal secondary metabolites as anticancer agents concerning cytotoxicity, apoptotic, genetic and histopathological profile. It was noticed that Asp. Terrius (Asp. T), Flavous (Asp. Fl) and Fumegatgus (Asp. Fu) induced variable toxic potential that was cell type, secondary metabolite type and concentration dependent. CaCo-2 cells showed less sensitivity than HuH-7 and in turn the IC50 was variable. Also, the apoptotic potential of Asp. species derived fungal secondary metabolites was proven via detection of up regulated pro apoptotic genes and down regulation of anti-apoptotic genes. The expression rate was cell type dependent. Concurrently apoptotic profile was accompanied with cellular DNA accumulation at the G2/M phase, an insignificant accumulation during the G0/G1 phase but there was not during the Pre-G1 and S phases. Also, there were a characteristic apoptotic features of treated cells presented as abnormal intra-nuclear eosinophyllic structures, necrotic cells with mixed euchromatin and heterochromatin, ruptured cell membranes, intranuclear eosinophyllic structures, apoptotic cells with irregular cellular and nuclear membranes, peripheral chromatin condensation and necrotic swollen cells with mixed euchromatin and heterochromatin. It can be concluded that A. secondary metabolites are promising agents can be used as a supplementary agents to current cancer drugs regimen applied.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"96 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82644043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Note on COVID-19","authors":"E. Dirican","doi":"10.37421/JCH.2020.11.E124","DOIUrl":"https://doi.org/10.37421/JCH.2020.11.E124","url":null,"abstract":"During 2019, we have published articles on various topics which include research, review, short communication and editorials.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"44 1","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76972191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concordance of Cytomorphological Features of Cervical Lymphadenitis Suspected for Mycobacterium Tuberculosis on Fine Needle Aspiration Biopsy with GeneXpert for Mycobacterium Tuberculosis on Aspirated Material","authors":"H. Mansur, M. Asif, M. T. Khadim, I. Khan, R. Ahmed, A. Azhar, M. Anwar","doi":"10.4172/2157-7099.1000526","DOIUrl":"https://doi.org/10.4172/2157-7099.1000526","url":null,"abstract":"Objective: The objective of the study was to correlate different cytomorphological presentations of cervical lymphadenitis suspected for Mycobacterium Tuberculosis (MTB) on Fine Needle Aspiration Cytology (FNAC) with MTB detection by geneXpert on aspirated material.Study Design: Comparative, cross sectional study.Place and Duration of Study: Department of Histopathology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, Pakistan, from January 2017 through November 2018.Methodology: Fine needle aspirates of total 100 patients with cervical lymphadenitis suspected for MTB were included in the study and the cytology was compared with geneXpert, keeping geneXpert as gold standard. After obtaining FNA aspirates from cervical lymph nodes, the smears were evaluated for various patterns of granulomatous inflammation. The aspirate was sent to the microbiology department for confirmation of presence of MTB by PCR (geneXpert), results of which were then compared. Data was analyzed using SPSS version 23. Descriptive statistics, frequencies and percentages were calculated.Results: A total of 100 samples of cervical lymphadenitis, in which MTB was suspected, were evaluated out of which 53 were males and 47 were females. The male to female ratio was 1.12:1. Mean age of population in study was 31 ± 15 years. Minimum age at presentation was 4 years and maximum age was 72 years. Maximum incidence of disease was seen in young patients between 15 and 40 years. On FNAC smears, a total of 61% (n=61) cases showed granulomas with caseous necrosis, 14% (n=14) cases showed abscess only, 10% cases (n=10) showed granulomas only, 10% (n=10) cases showed granulomas with abscess while 5% (n=5) cases showed caseous necrosis only. A total of 78 cases were positive for MTB while 22 cases were negative when compared with molecular analysis on aspirated material. Positivity for MTB by geneXpert was seen in 93% (n=57) cases with both caseous necrosis and granulomas, 80% (n=4) cases with caseous necrosis only, 80% (n=10) cases having granulomas along with neutrophilic abscess, 70% (n=7) cases showing only granulomas and 14% (n=2) cases with neutrophilic abscess only (p value<0.05). The sensitivity of FNAC was 97% and specificity was 54.Positive predictive value (PPV) was 88% and Negative predictive value (NPV) was 85%.Conclusion: MTB was detected by GeneXpert in a significant percentage of the FNAC samples included in our study. FNAC is a rapid, safe, inexpensive, easily available, minimally invasive, outpatient procedure and GeneXpert can be performed on the aspirated material for the diagnosis of cervical lymphadenitis suspected for MTB infection. This can aid the clinicians in timely initiation of Anti Tuberculous Treatment (ATT) in our country where tuberculosis is rampant and advanced diagnostic facilities are not easily accessible.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"2 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2019-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79064971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Clinic-Pathological, Molecular Features and PD-L1 Status in a Series of Non-small Cell Lung Cancers: Are Real Life Data Similar to Clinical Trials results?","authors":"M. Elshiekh, A. Mani, R. Kitson, E. Josephides, A. Clifford, R. Rieu, S. Desai, N. Gupta, M. Berry, S. Bloch, C. Ross, J. Anderson, J. Nandi, M. Roddie, S. Copley, A. Denton, O. Hatcher, D. Power, C. Lewanski, T. Newsom-Davis, P. Viola","doi":"10.4172/2157-7099.1000541","DOIUrl":"https://doi.org/10.4172/2157-7099.1000541","url":null,"abstract":"Objective: Immunotherapy is a promising treatment option for a subset of lung cancers as it utilizes the host’s own immune system to attack tumors cells. Selection of patients who are likely to respond to immunotherapy is based on PD-L1 expression, a specific biomarker. Clinic-pathological correlation of PD-L1 status and NSCLC has been explored in several studies and large clinical trials. However, there is discrepancy of data as several antibodies are available. We looked at a series of lung tumors to study the association between PD-L1 expression and patient characteristics in our daily setting to improve the selection of patients more likely to express this marker. Results were compared to those available in literature using the same antibody.Methods: We analysed PD-L1 status (using Dako clone 22C3) in 170 non-small cell lung cancers and correlated their PD-L1 status with clinical, pathological and molecular characteristics focusing in particular on EGFR and ALK status.Results: We found a statistically significant association between PD-L1 status, histological pattern in theadenocarcinomas subtype and stage of the disease.Conclusion: Our results support the current findings that PD-L1 expression more frequently occurs in advanced stage disease and certain histological pattern. Our data also confirmed longer survival in PD-L1 positive patients.Highlights• Immunotherapy is a promising option for the treatment of NSCLC• PD-L1 status detected by immunohistochemistry is linked to immunotherapy response.• There are many clones available but only 22C3 is approved as companion diagnostic• Patient selection can be affected by the antibody used.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"16 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2019-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78362107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thyroid Metastasis of Squamous Cell Carcinoma in an Unsuspected Patient","authors":"D. Sa, Meena Pa, Radhika Pp","doi":"10.4172/2157-7099.1000540","DOIUrl":"https://doi.org/10.4172/2157-7099.1000540","url":null,"abstract":"Malignancies metastasizing to thyroid are a very rare phenomenon. Out of them all, diagnosis of metastatic squamous cell carcinoma in thyroid can be difficult to establish as in thyroid there can also be a primary thyroid squamous cell carcinoma SCC.We herein report a rare case of a 28-year old man with metastasis of oral SCC the thyroid gland. He was operated six months back, took incomplete cycles of adjuvant chemo-radiotherapy. On FDG-PET CT was found to have metabolically active lesions in right lobe of thyroid along with right paratracheal and bilateral cervical nodes.Fine needle aspiration cytology (FNAC) smears from thyroid showed malignant squamous cells. Considering various factors like previous history, histopathology, present FDG-PET scan findings and present cytology diagnosis was concluded to be metastatic over primary thyroid SCC.It is necessary to differentiate between primary or metastatic SCC in thyroid as they are different in clinical and prognostic behaviour. Primary squamous cell carcinoma is very aggressive with a poor prognosis.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"84 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2019-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83872818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of N-Acetyl Cysteine on Doxorubicin Induced Cardiotoxicity in Adult Male Albino Rat: Histological Study","authors":"H. K. Mostafa, M. Raafat","doi":"10.4172/2157-7099.1000536","DOIUrl":"https://doi.org/10.4172/2157-7099.1000536","url":null,"abstract":"Introduction: Cardiotoxicity is one of the most important causes that limit the use of Doxorubicin (DOX) in treatment of the cancer. N-acetylcysteine (NAC) is an antioxidant substance that protects different cellular organelles from free radicals. \u0000The aim of the work: To shed the spot on the role of NAC on cardiotoxicity induced by DOX. \u0000Materials and methods: Forty adult male albino rats were divided into three groups. Group I: it was formed of 20 animals served as a control group. Further, it was divided into two subgroups; Subgroup Ia: formed of 10 animals received physiologic saline and Subgroup Ib: formed of 10 animals received NAC. Group II: formed of 10 animals that received DOX dissolved in normal saline. Group III: formed of 10 animals that received DOX similar togroup II and NAC similar to subgroup Ib. At the end of the second week, all animals were sacrificed; the heart specimens were dissected out and processed to light and electron microscopic examination. Morphometric and statistical analysis was also done. \u0000Results: Light microscopic examination of group II showed deeply stained cardiac muscle fibers and congested coronary vessels. Distorted cardiac muscle fibers and deeply stained nuclei were also observed. Moreover, cellular infiltration was also observed among cardiac muscle fibers. Apparent increase in greenish collagen fibers was seen between cardiac muscle fibers by Masson's trichrome. Electron microscopic examination of group II showed the cardiac muscle with thinning out of some myofibrils, vacuolations of the sarcoplasm and irregular wavy nuclear envelop. Telocytes appeared between cardiac muscle fibers. Group III showed improvement of the cardiac muscle by light and electron microscope with minimal vacuolation in the cardiac muscle. Morphometric and statistical analysis confirmed the histological results. \u0000Conclusion: The present study demonstrated that the administration of N acetyl cysteine could protect against cardiaotoxicity induced by doxorubicin.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81078937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological Study on Possible Therapeutic Effect of BM-MSCs on Healing of Lung Fibrosis Induced by CCl 4 with Reference to Macrophage Plasticity","authors":"Ghada G Hamam, M. Raafat, H. K. Mostafa","doi":"10.4172/2157-7099.1000537","DOIUrl":"https://doi.org/10.4172/2157-7099.1000537","url":null,"abstract":"Background: Lungs are susceptible to several types of toxins. Macrophages may play role in lung fibrosis. Mesenchymal stem cells (MSCs) have several valuable functions that make them a promising therapeutic option in the field of regenerative medicine. Objectives: Investigate possible therapeutic effects of bone marrow (BM)-MSCs on lung injury induced by carbon tetrachloride (CCl4) in adult rats with reference to role of macrophages plasticity. Material and methods: Forty-five adult male albino Wistar rats were divided into four groups. Group I (control). Group II (CCl4): rats received intraperitoneal injection of CCl4 (0.1 ml/100 g body weight) twice weekly for two weeks. Group III (BM-MSCs treated) received single injection of BM-MSCs after last injection of CCl4 and were left for further two weeks. Group IV (recovery): were left for two weeks after last injection of CCl4. At the end of experiment, all rats were sacrificed. Lung specimens were processed and subjected to H&E, Mallory`s trichrome, CD68, toluidine blue, transmission electron microscope and histomorphometric and statistical studies. Results: CCl4 and recovery groups showed thickening of interalveolar septa with mononuclear cellular infiltration, dissolved lamellar bodies in pneumocytes type-II and appearance of foamy macrophages. Significant increase in mean area percentage of collagen fibers, and mean number of CD68 macrophages were also noticed. BM-MSCs improved these histological changes with a significant increase number in pneumocyte type-II. Conclusions: CCl4 caused lung injury that was associated with inflammation, increased number of macrophages, and collagen fiber deposition. Treatment with BM-MSCs alleviates these changes and could be used in regenerative medicine.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"112 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87889696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological Preparation Technique of Blood Derivative Injectable Platelet-Rich Fibrin (I-Prf) for Microscopic Analyzes","authors":"H. Varela, R. F. Araújo, R. C. Vasconcelos, V. B. Garcia, L. B. Souza, A. Araújo","doi":"10.4172/2157-7099.1000506","DOIUrl":"https://doi.org/10.4172/2157-7099.1000506","url":null,"abstract":"Objective: The objective of the present study was to describe the histological preparation technique of blood derivative injectable platelet-rich fibrin (i-PRF) for microscopic analyzes.Materials and methods: Blood samples were collected from 15 volunteers to prepare i-PRF samples. The difference between i-PRF and oral mucosa tissue experimental protocol for histological preparation was elaborated in Step 1 - fixation, with 01 hour for i-PRF fixation and 24 hours for oral mucosa tissue. The other histological preparation protocol steps used for the processing and staining of the specimens were similar to that used for oral mucosa tissue. Tissue and i-PRF samples were analyzed by immunofluorescence, immunohistochemistry and microscopic histology.Results: In comparing i-PRF and oral mucosal tissue, it was observed that both had similarities in morphology and coloration of the visualized structures. The technique demonstrates immunostaining regulation of TGF-β from i- PRF. VEGF was fully detectable using the immunofluorescence technique applied to i-PRF. The method showed a fundamental advantage in reducing the fixation time, since the established fixation time in oral mucosa tissue is at least 24 hours. For the i-PRF, it was observed that the fixation was 01 hour, so the reduced fixation time shows an advantage in reducing the total working time without compromising the quality of the analyzed samples.Conclusions: The method showed a distinct advantage in reducing the fixation time of i-PRF. These initial analyses revealed that the slow polymerization during i-PRF preparation generates a blood derivative with a special fibrin network rich in platelets and leukocytes, and the presence of immunostaining for TGF-β and immunofluorescence of VEGF.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"34 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2018-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86606396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}