Journal of Clinical Virology最新文献

{"title":"Coordinated implementation of a conventional PCR assay to detect all Ebola and Marburg virus species in a European laboratory network","authors":"K.C. Heimsch ,&nbsp;T. Bleicker ,&nbsp;T.D. Best ,&nbsp;L.D. Presser ,&nbsp;R. Molenkamp ,&nbsp;A.J. Jääskeläinen ,&nbsp;A. Milewska ,&nbsp;J. Šmahelová ,&nbsp;C. Baronti ,&nbsp;S. Pappa ,&nbsp;I. Tabain ,&nbsp;R. Cordeiro ,&nbsp;G. Marsili ,&nbsp;K. Huik ,&nbsp;V. Pinho dos Reis ,&nbsp;L. Barzon ,&nbsp;P. Maes ,&nbsp;C. Drosten ,&nbsp;V.M. Corman","doi":"10.1016/j.jcv.2025.105808","DOIUrl":"10.1016/j.jcv.2025.105808","url":null,"abstract":"<div><h3>Background</h3><div>Filoviruses, including Ebola and Marburg viruses, cause severe hemorrhagic fever in humans and primates. These viruses pose significant threats to public health, making rapid and sensitive detection critical for controlling outbreaks. We developed and validated a hemi-nested generic PanFilo assay to detect all Ebola virus species, Marburg viruses, and recently discovered bat filoviruses. This assay was deployed to 15 European laboratories and evaluated through testing of eight non-infectious samples.</div></div><div><h3>Objectives</h3><div>Laboratories were asked to determine the detection limit of positive controls and test all samples using the assay provided. The deployed assay enables direct Nanopore sequencing of PCR products, by using tagged primers during the second round of PCR. Sequencing of the samples was carried out on a voluntary basis.</div></div><div><h3>Results</h3><div>Multicenter validation revealed a 95 % limit of detection of 5309 RNA copies/µL for Ebola, 10,273 copies/µL for Marburg, and 2145 copies/µL for Mengla virus. In an implementation quality assessment, 93.3 % (84/90) of samples containing filovirus RNA were correctly identified and 100 % (30/30) of filovirus-negative samples were correctly identified. Thirteen laboratories sequenced PCR products, with nine identifying all positive samples correctly.</div></div><div><h3>Conclusion</h3><div>The assay enables rapid and reliable detection of filoviruses, with sequencing capabilities for identifying both known and novel variants. This assay might be used for detection during the initial phase of an emerging filovirus outbreak, before a specific assay has been developed. However, our distribution across 15 laboratories revealed variability challenges due to reagents, human performance, and sequencing capacity, emphasizing the need for more training and standardization.</div></div>","PeriodicalId":15517,"journal":{"name":"Journal of Clinical Virology","volume":"178 ","pages":"Article 105808"},"PeriodicalIF":4.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144185662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction notice to “Evaluating the efficacy and safety of a novel prophylactic nasal spray in the prevention of SARS-CoV-2 infection: A multi-centre, double blind, placebo-controlled, randomised trial” [J. Clin. Virol. 155C (2022) 105248]","authors":"Damian Balmforth ,&nbsp;James A. Swales ,&nbsp;Laurence Silpa ,&nbsp;Alan Dunton ,&nbsp;Kay E. Davies ,&nbsp;Stephen G. Davies ,&nbsp;Archana Kamath ,&nbsp;Jayanti Gupta ,&nbsp;Sandeep Gupta ,&nbsp;M. Abid Masood ,&nbsp;Áine McKnight ,&nbsp;Doug Rees ,&nbsp;Angela J. Russell ,&nbsp;Manu Jaggi ,&nbsp;Rakesh Uppal","doi":"10.1016/j.jcv.2025.105782","DOIUrl":"10.1016/j.jcv.2025.105782","url":null,"abstract":"","PeriodicalId":15517,"journal":{"name":"Journal of Clinical Virology","volume":"178 ","pages":"Article 105782"},"PeriodicalIF":4.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Post-mortem study of endemic human coronaviruses (HCoV-NL63, OC43, 229E and HKU-1) in deaths of children under five in low- and middle-income countries: Findings from the Child Health and Mortality Prevention Surveillance (CHAMPS) study","authors":"Vicky Baillie ,&nbsp;Ziyaad Dangor ,&nbsp;Dianna M. Blau ,&nbsp;Sana Mahtab ,&nbsp;Jeanie du Toit ,&nbsp;Nega Assefa ,&nbsp;Joseph Oundo ,&nbsp;Zelalem Teklemariam Kidanemariam ,&nbsp;J. Anthony G. Scott ,&nbsp;Soter Ameh ,&nbsp;Ikechukwu Udo Ogbuanu ,&nbsp;Julius Ojulong ,&nbsp;James Bunn ,&nbsp;Karen L. Kotloff ,&nbsp;Samba O. Sow ,&nbsp;Milagritos D. Tapia ,&nbsp;Adama Mamby Keita ,&nbsp;Marcelino Garrine ,&nbsp;Inacio Mandomando ,&nbsp;Rosauro Varo ,&nbsp;Shabir A. Madhi","doi":"10.1016/j.jcv.2025.105804","DOIUrl":"10.1016/j.jcv.2025.105804","url":null,"abstract":"<div><h3>Background</h3><div>Endemic human coronaviruses (HCoV-229E, HKU1, NL63, and OC43) are common causes of mild or asymptomatic respiratory infections in children but are considered rare causes of death.</div></div><div><h3>Methods</h3><div>We evaluated pediatric deaths from January 2017 through December 2022. A panel of experts determined the cause of death (CoD) by reviewing available data, including pathological and molecular findings from minimally invasive tissue sampling (lung tissues, blood, CSF, and nasopharyngeal swabs), clinical records, and verbal autopsies.</div></div><div><h3>Results</h3><div>Endemic HCoV were detected in the respiratory samples of 3 % (n = 86/3357) of enrolled decedents: 1 % (n = 12/2043) of neonates, 5 % (n = 35/681) of infants and 6 % (n = 39/633) of children deaths. However, HCoVs were attributed as the CoD in only two cases — both involving young infants with underlying birth defects and severe wasting, who succumbed to polymicrobial hospital-acquired infections involving <em>HCoV-OC43</em>, <em>Klebsiella pneumoniae</em>, and <em>Acinetobacter baumannii</em>. Amongst the remaining 84 decedents in whom an HCoV was detected, 82 % (n = 69/84; median Ct of 25.34; range: 15.28–36.17) were deaths attributed to other infections, including 54 % (n = 32/69; median Ct of 23.86; range: 15.28–35.2) with lower respiratory infections determined to be the CoD. The bulk of these deaths (96 %, n = 66/69) were attributed to other pathogens – <em>Plasmodium falciparum</em> (27 %, n = 19/69), <em>K. pneumoniae</em> (23 %, n = 16/69), <em>Streptococcus pneumoniae</em> (20 %, n = 14/69), <em>Escherichia coli</em> (16 %, n = 11/69) and Cytomegalovirus (10 %, n = 7/69).</div></div><div><h3>Conclusion</h3><div>Although endemic HCoV was identified in children who died of respiratory infections, it was rarely attributed to being in the CoD. Nevertheless, further research is warranted to explore the potential role of HCoVs in LRTI pathogenesis and their impact on facilitating more pathogenic infections.</div></div>","PeriodicalId":15517,"journal":{"name":"Journal of Clinical Virology","volume":"178 ","pages":"Article 105804"},"PeriodicalIF":4.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144189833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evolving landscape of Norovirus GII genotypes in Asia: A systematic review and meta-analysis","authors":"Ashutosh Kumar Singh ,&nbsp;Juhi Nagar ,&nbsp;Akansha Tandekar ,&nbsp;Surya Singh ,&nbsp;Vishal Diwan ,&nbsp;Greeshma C. Ravindran ,&nbsp;Rajnarayan R. Tiwari ,&nbsp;Pradyumna Kumar Mishra ,&nbsp;Ram Kumar Nema","doi":"10.1016/j.jcv.2025.105809","DOIUrl":"10.1016/j.jcv.2025.105809","url":null,"abstract":"<div><h3>Background</h3><div>Norovirus is a leading cause of acute gastroenteritis globally, with genotypic variation influencing epidemiology and disease outcomes. Understanding the distribution and prevalence of different Norovirus genotypes is crucial for public health surveillance and intervention strategies.</div></div><div><h3>Methods</h3><div>We conducted a systematic review of Norovirus genotypes in Asia from 2000 to 2023. The review adhered to PRISMA Guidelines and was registered with PROSPERO (ID CRD42024572647). We extracted data on the prevalence of 22 genotypes and their genetic variations over time.</div></div><div><h3>Results</h3><div>The review highlighted Norovirus GII.4 as highly prevalent across Asia, particularly in India, Taiwan, Vietnam, and China. GII.2 dominated Indonesia, GII.3 prevailed in Malaysia, Russia, and Bangladesh, GII.7 in Bangladesh, and GII.17 in China, Taiwan, and Nepal, with notable epidemiological shifts between 2012 and 2016 and GII.4 resurgence from 2017.</div></div><div><h3>Conclusion</h3><div>The study highlights the dynamic nature of Norovirus genotypic distribution in Asia, with both persistent dominance of certain genotypes and notable regional variations. The cyclic patterns of genotype prevalence, particularly the shifts between GII.4 and GII.17, underline the need for ongoing genotypic surveillance to inform targeted public health responses. The data underscores the complexity of Norovirus epidemiology and the importance of maintaining vigilance in monitoring emerging and re-emerging strains.</div></div>","PeriodicalId":15517,"journal":{"name":"Journal of Clinical Virology","volume":"179 ","pages":"Article 105809"},"PeriodicalIF":4.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144205631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implications of subtyping influenza A amongst H5N1 surveillance efforts","authors":"Samuel M. Goodfellow,&nbsp;Jennifer Dien Bard,&nbsp;Cristina Costales","doi":"10.1016/j.jcv.2025.105810","DOIUrl":"10.1016/j.jcv.2025.105810","url":null,"abstract":"","PeriodicalId":15517,"journal":{"name":"Journal of Clinical Virology","volume":"179 ","pages":"Article 105810"},"PeriodicalIF":4.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology and genotypic diversity of rhinovirus in school-age children with acute respiratory illnesses seeking medical care","authors":"Dithi Banerjee ,&nbsp;Jennifer E. Schuster ,&nbsp;Claire M. Midgley ,&nbsp;Brian Lee ,&nbsp;Mary Moffatt ,&nbsp;Joana Y. Lively ,&nbsp;Ariana P. Toepfer ,&nbsp;Geoffrey A. Weinberg ,&nbsp;Julie A. Boom ,&nbsp;Leila C. Sahni ,&nbsp;Vasanthi Avadhanula ,&nbsp;Pedro A. Piedra ,&nbsp;Mary Allen Staat ,&nbsp;Daniel C. Payne ,&nbsp;Natasha Halasa ,&nbsp;John V. Williams ,&nbsp;Robert W. Hickey ,&nbsp;Marian G. Michaels ,&nbsp;Janet A. Englund ,&nbsp;Eileen J. Klein ,&nbsp;Rangaraj Selvarangan","doi":"10.1016/j.jcv.2025.105806","DOIUrl":"10.1016/j.jcv.2025.105806","url":null,"abstract":"<div><h3>Background</h3><div>Rhinovirus (RV) associated acute respiratory illness (ARI) data come mostly from infants and young children. We present data from 5 to 17-year-olds to characterize RV species A, B and C.</div></div><div><h3>Methods</h3><div>During December 1, 2016–Nov 30, 2017, seven U.S. New Vaccine Surveillance Network (NVSN) sites performed active pediatric ARI surveillance of inpatients (IP) and emergency department (ED) patients using molecular platforms to detect multiple respiratory pathogens. RV or RV/enterovirus (EV) positive specimens without co-detections were sequenced. Demographic and clinical data collected via parent interview and chart review were analyzed descriptively by RV species, month, and hospital setting, using chi-squared tests for comparisons.</div></div><div><h3>Results</h3><div>RV or RV/EV was detected in 581/2298 (25.3 %) ARI patients; 529 were single detections, 516 of these had sufficient sample for sequencing, and 420 (81.4 %) yielded sequence results: RV-A (183, 35.5 %), RV-B (16, 3.1 %), RV-C (210, 40.7 %), non-typeable RV (2, 0.4 %), and EV (9, 1.7 %). Among 52 RV-A, 8 RV-B and 44 RV-C unique types identified, A49 (32, 61.5 %), B6 (5, 62.5 %) and C15 (22, 50 %) were predominant. History of asthma was reported in 65.4 % RV-A, 50 % RV-B and 78.5 % RV-C patients (<em>p</em> = 0.005). Hospitalization occurred in 63.4 % RV-A, 37.5 % RV-B and 71.4 % RV-C patients (<em>p</em> = 0.012). RV-C detections peaked during winter and RV-A peaked during summer-fall.</div></div><div><h3>Conclusions</h3><div>RV exhibited genetic diversity in 5–17-year-old ARI patients, and circulation differed by RV species. Among children seeking care in ED or hospital settings, with confirmed RV or RV/EV single detections, hospitalization was more common with RV-A and -C than RV-B.</div></div>","PeriodicalId":15517,"journal":{"name":"Journal of Clinical Virology","volume":"179 ","pages":"Article 105806"},"PeriodicalIF":4.0,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly specific serological diagnosis of Borna disease virus 1 (BoDV-1) and variegated squirrel bornavirus 1 (VSBV-1) encephalitis by novel antibody isotype assay with multiple viral antigens","authors":"Mathias Schlegel ,&nbsp;Petra Allartz ,&nbsp;Ariane Wenzel ,&nbsp;Thomas Faupel ,&nbsp;Klemens Loester ,&nbsp;Dennis Tappe","doi":"10.1016/j.jcv.2025.105803","DOIUrl":"10.1016/j.jcv.2025.105803","url":null,"abstract":"<div><h3>Background</h3><div>Human bornavirus encephalitis is an emerging, severe and nearly uniformly fatal zoonotic disease in Germany. The etiological pathogens so far encompass the Borna disease virus 1 (BoDV-1) and the variegated squirrel bornavirus 1 (VSBV-1). While BoDV-1 is at least harbored by the white-toothed shrew (<em>Crocidura leucodon</em>) as a natural reservoir and autochthonous in Germany, VSBV-1 has been detected in captive exotic squirrels with an unknown geographical origin. Clinically, a rapid progression is typical for both forms of bornavirus encephalitis, however, medical awareness is low, and therefore treatment attempts are notably delayed. Diagnosis relies on symptomatology, epidemiology, imaging, and virologic testing. One cornerstone of laboratory diagnosis is serology with limitations in sensitivity and specificity.</div></div><div><h3>Objectives</h3><div>Here, we describe a newly developed spot immunoassay using recombinant BoDV-1 nucleoprotein (N), phosphoprotein (P), accessory protein X (X), and glycoprotein (GP) to detect bornavirus-reactive IgG, IgM and IgA antibodies.</div></div><div><h3>Study design</h3><div>A comparatively large cohort encompassing 14 patients with BoDV-1 encephalitis and one individual with VSBV-1 encephalitis were tested. In addition, 241 patients with encephalitis of unknown etiology, 58 interference samples, as well as 40 blood donor samples were analyzed.</div></div><div><h3>Results</h3><div>The combined use of different antibody isotype-specific conjugates with four different BoDV-1-specific proteins (N/P/X/GP) while employing a newly developed evaluation scheme enabled a highly specific (97–100 %) diagnosis in patients with either form of bornavirus encephalitis, with a sensitivity of up to 92 %.</div></div><div><h3>Conclusions</h3><div>The novel spot immunoassay is an easy-to-use approach for the specific and sensitive serological diagnosis of human bornavirus encephalitis.</div></div>","PeriodicalId":15517,"journal":{"name":"Journal of Clinical Virology","volume":"178 ","pages":"Article 105803"},"PeriodicalIF":4.0,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144099235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of Human Metapneumovirus from clinical specimen in human organoid-derived bronchial cell cultures is superior to isolation in monolayer cell line cultures","authors":"Pau Ribó-Molina,&nbsp;Stefan van Nieuwkoop,&nbsp;Mathis Funk ,&nbsp;Babs E. Verstrepen,&nbsp;Jeroen J.A. van Kampen,&nbsp;Ron A.M. Fouchier,&nbsp;Bernadette G. van den Hoogen","doi":"10.1016/j.jcv.2025.105805","DOIUrl":"10.1016/j.jcv.2025.105805","url":null,"abstract":"<div><h3>Background</h3><div>Human Metapneumovirus (HMPV) is a causative agent of respiratory tract infections (RTI) in children and adults. HMPV is a member of the <em>Pneumoviridae</em> family for which circulation of two serotypes, A and B, has been reported. HMPV isolation in standard monolayer cell lines is not always successful. Recently, it was shown that upon inoculation of human organoid-derived bronchial (ODB) cultures, HMPV primarily targeted the ciliated cells, similar as observed in experimentally infected animals. These observations lead to the hypothesis that isolation of virus from clinical specimen in this ODB model could be more successful than in standard monolayer cultures.</div></div><div><h3>Methods</h3><div>This study compared the efficiency of isolation of HMPV from 36 clinical samples in human ODB cultures with that in monolayers of Vero-118 cells.</div></div><div><h3>Results</h3><div>A total of 27 isolates (8 HMPV A and 19 HMPV B) were obtained in the ODB cultures, after one passage, whereas 21 isolates (9 HMPV A and 12 HMPV B) were obtained after one or two passages in Vero-118 cells.</div></div><div><h3>Conclusions</h3><div>Overall, the isolation efficiency of serotype A HMPV was comparable in both models, while isolation of serotype B viruses was profoundly more efficient in the ODB cultures than in Vero-118 cells, suggesting that primary cultures expressing ciliated cells should be considered as a superior isolation method for HMPV from clinical specimens.</div></div>","PeriodicalId":15517,"journal":{"name":"Journal of Clinical Virology","volume":"178 ","pages":"Article 105805"},"PeriodicalIF":4.0,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of polyethylene glycol precipitation and ultracentrifugation to enhance the sensitivity of hepatitis B virus DNA detection","authors":"Michael X. Fu ,&nbsp;Osmany Larralde ,&nbsp;Richard Mayne ,&nbsp;Kai Kean ,&nbsp;Kaitlin Reid ,&nbsp;Monique Andersson ,&nbsp;Tanya Golubchik ,&nbsp;Jane A. McKeating ,&nbsp;Lisa Jarvis ,&nbsp;William L. Irving ,&nbsp;Peter Simmonds ,&nbsp;Heli Harvala","doi":"10.1016/j.jcv.2025.105802","DOIUrl":"10.1016/j.jcv.2025.105802","url":null,"abstract":"<div><h3>Background</h3><div>Sensitive molecular detection of hepatitis B virus (HBV) DNA is crucial for diagnosing and managing occult hepatitis. To improve the sensitivity of HBV DNA detection, we compared the effectiveness of polyethylene glycol (PEG) precipitation and ultracentrifugation to concentrate DNA prior to extraction.</div></div><div><h3>Methods</h3><div>Twenty-three HBV DNA-positive samples with low viral loads were compared between the extraction of standard (0.2 mL) and larger volumes (5 mL) of plasma, through PEG precipitation of 10 mL and 20 mL of plasma, and ultracentrifugation from 35 mL of plasma. The effectiveness of the methods for HBV DNA detection was assayed by quantitative PCR. For genetic characterisation, Sanger sequencing of amplicons and targeted Illumina sequencing were used. Costs, sample capacities, and turnaround times were compared.</div></div><div><h3>Results</h3><div>DNA was detected in a greater number of samples using PEG and ultracentrifugation (detecting up to all 23 samples) compared to more standard extraction methods (detecting at least 18 samples). Efficiencies of recovery of HBV DNA from samples were comparable in all concentration methods. HBV and other DNA viruses, such as human herpesviruses and anelloviruses, were detected in samples and at higher read counts with PEG concentration than without. The availability, cost, relative simplicity, and throughput of PEG precipitation conferred further advantages to ultracentrifugation.</div></div><div><h3>Conclusions</h3><div>PEG precipitation from large volumes of plasma is a practical and economical alternative to ultracentrifugation and could be a similarly effective concentration method for low viral load samples in blood donation and clinical virology laboratories.</div></div>","PeriodicalId":15517,"journal":{"name":"Journal of Clinical Virology","volume":"178 ","pages":"Article 105802"},"PeriodicalIF":4.0,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143916907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of mid-turbinate nasal and combined nasal-throat specimen types for detection of respiratory viruses in children","authors":"Irem Onalan ,&nbsp;Zheyi Teoh ,&nbsp;Sarah L. Steele ,&nbsp;Eileen J. Klein ,&nbsp;Bonnie Strelitz ,&nbsp;Kirsten Lacombe ,&nbsp;Erin M. Sullivan ,&nbsp;Arun K. Nalla ,&nbsp;Danielle M. Zerr ,&nbsp;Janet A. Englund","doi":"10.1016/j.jcv.2025.105801","DOIUrl":"10.1016/j.jcv.2025.105801","url":null,"abstract":"<div><h3>Background</h3><div>The source of respiratory specimens may impact the detection of respiratory viruses. It may also have implications for research participant recruitment, caregiver acceptability due to concerns for patient comfort, and potential risk of aerosolization during epidemics/pandemics.</div></div><div><h3>Objective</h3><div>To determine the impact of collecting a throat swab (TS) in addition to a mid-turbinate nasal swab (MTS) by comparing agreement of viral detection and relative viral loads.</div></div><div><h3>Study design</h3><div>We reviewed molecular detection results from 2548 children enrolled in the New Vaccine Surveillance Network at Seattle Children’s Hospital from 11/2015–05/2019. Participants with a clinical MTS who agreed to collection of a combined TS and MTS (TS&amp;MTS) for research were included. All specimens were tested using FilmArray^R Respiratory Panel (Biofire Diagnostics). Viral detection from MTS and TS&amp;MTS were compared. Relative viral loads were compared between specimens with concordant (same viruses detected) and discordant (different or additional viruses detected) results.</div></div><div><h3>Results</h3><div>Results from 743 participants with clinical MTS and research TS&amp;MTS specimens were compared; Viral detections were similar between the two groups, including 596 (80.2 %) paired results that were concordant. The most common discordant viruses were rhinovirus/enterovirus, respiratory syncytial virus, and adenovirus. Mean relative viral loads were lower in discordant specimens compared to concordant specimens, regardless of specimen source.</div></div><div><h3>Conclusion</h3><div>Comparison of clinical and research specimens revealed that respiratory viral detection was similar with or without an added TS. Lower relative viral loads of discordant specimens suggest that a combined TS&amp;MTS may not improve viral detection for clinically significant pathogens.</div></div>","PeriodicalId":15517,"journal":{"name":"Journal of Clinical Virology","volume":"178 ","pages":"Article 105801"},"PeriodicalIF":4.0,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}