Isolation of Human Metapneumovirus from clinical specimen in human organoid-derived bronchial cell cultures is superior to isolation in monolayer cell line cultures

IF 4 3区医学 Q2 VIROLOGY

Journal of Clinical Virology Pub Date : 2025-05-15 DOI:10.1016/j.jcv.2025.105805

Pau Ribó-Molina, Stefan van Nieuwkoop, Mathis Funk , Babs E. Verstrepen, Jeroen J.A. van Kampen, Ron A.M. Fouchier, Bernadette G. van den Hoogen

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引用次数: 0

Abstract

Background

Human Metapneumovirus (HMPV) is a causative agent of respiratory tract infections (RTI) in children and adults. HMPV is a member of the Pneumoviridae family for which circulation of two serotypes, A and B, has been reported. HMPV isolation in standard monolayer cell lines is not always successful. Recently, it was shown that upon inoculation of human organoid-derived bronchial (ODB) cultures, HMPV primarily targeted the ciliated cells, similar as observed in experimentally infected animals. These observations lead to the hypothesis that isolation of virus from clinical specimen in this ODB model could be more successful than in standard monolayer cultures.

Methods

This study compared the efficiency of isolation of HMPV from 36 clinical samples in human ODB cultures with that in monolayers of Vero-118 cells.

Results

A total of 27 isolates (8 HMPV A and 19 HMPV B) were obtained in the ODB cultures, after one passage, whereas 21 isolates (9 HMPV A and 12 HMPV B) were obtained after one or two passages in Vero-118 cells.

Conclusions

Overall, the isolation efficiency of serotype A HMPV was comparable in both models, while isolation of serotype B viruses was profoundly more efficient in the ODB cultures than in Vero-118 cells, suggesting that primary cultures expressing ciliated cells should be considered as a superior isolation method for HMPV from clinical specimens.

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从人类器官支气管细胞培养的临床标本中分离人偏肺病毒优于从单层细胞系培养中分离

背景：人偏肺病毒（HMPV）是儿童和成人呼吸道感染（RTI）的病原体。HMPV是肺炎病毒科的一员，已报告有两种血清型a和B的传播。在标准单层细胞系中分离HMPV并不总是成功的。最近，研究表明，接种人类器官衍生支气管（ODB）培养物后，HMPV主要针对纤毛细胞，这与在实验感染动物中观察到的情况相似。这些观察结果导致假设，在这种ODB模型中从临床标本中分离病毒可能比在标准单层培养中更成功。方法本研究比较了从36个临床标本中分离HMPV的效率和从Vero-118细胞单层中分离HMPV的效率。结果ODB培养1代共分离到HMPV A 8株、HMPV B 19株，而Vero-118细胞1、2代共分离到HMPV A 9株、HMPV B 12株。结论在两种模型中，血清A型HMPV的分离效率相当，而血清B型HMPV在ODB培养物中分离效率远高于在Vero-118细胞中分离效率，提示表达纤毛细胞的原代培养物可作为临床标本中分离HMPV的较优方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Clinical Virology 医学-病毒学

CiteScore

22.70

自引率

1.10%

发文量

149

审稿时长

24 days

期刊介绍： The Journal of Clinical Virology, an esteemed international publication, serves as the official journal for both the Pan American Society for Clinical Virology and The European Society for Clinical Virology. Dedicated to advancing the understanding of human virology in clinical settings, the Journal of Clinical Virology focuses on disseminating research papers and reviews pertaining to the clinical aspects of virology. Its scope encompasses articles discussing diagnostic methodologies and virus-induced clinical conditions, with an emphasis on practicality and relevance to clinical practice. The journal publishes on topics that include: • new diagnostic technologies • nucleic acid amplification and serologic testing • targeted and metagenomic next-generation sequencing • emerging pandemic viral threats • respiratory viruses • transplant viruses • chronic viral infections • cancer-associated viruses • gastrointestinal viruses • central nervous system viruses • one health (excludes animal health)