儿童中鼻甲鼻与合并鼻咽标本类型检测呼吸道病毒的比较

IF 4 3区医学 Q2 VIROLOGY

Journal of Clinical Virology Pub Date : 2025-04-29 DOI:10.1016/j.jcv.2025.105801

Irem Onalan , Zheyi Teoh , Sarah L. Steele , Eileen J. Klein , Bonnie Strelitz , Kirsten Lacombe , Erin M. Sullivan , Arun K. Nalla , Danielle M. Zerr , Janet A. Englund

{"title":"儿童中鼻甲鼻与合并鼻咽标本类型检测呼吸道病毒的比较","authors":"Irem Onalan , Zheyi Teoh , Sarah L. Steele , Eileen J. Klein , Bonnie Strelitz , Kirsten Lacombe , Erin M. Sullivan , Arun K. Nalla , Danielle M. Zerr , Janet A. Englund","doi":"10.1016/j.jcv.2025.105801","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>The source of respiratory specimens may impact the detection of respiratory viruses. It may also have implications for research participant recruitment, caregiver acceptability due to concerns for patient comfort, and potential risk of aerosolization during epidemics/pandemics.</div></div><div><h3>Objective</h3><div>To determine the impact of collecting a throat swab (TS) in addition to a mid-turbinate nasal swab (MTS) by comparing agreement of viral detection and relative viral loads.</div></div><div><h3>Study design</h3><div>We reviewed molecular detection results from 2548 children enrolled in the New Vaccine Surveillance Network at Seattle Children’s Hospital from 11/2015–05/2019. Participants with a clinical MTS who agreed to collection of a combined TS and MTS (TS&MTS) for research were included. All specimens were tested using FilmArray<sup>R</sup> Respiratory Panel (Biofire Diagnostics). Viral detection from MTS and TS&MTS were compared. Relative viral loads were compared between specimens with concordant (same viruses detected) and discordant (different or additional viruses detected) results.</div></div><div><h3>Results</h3><div>Results from 743 participants with clinical MTS and research TS&MTS specimens were compared; Viral detections were similar between the two groups, including 596 (80.2 %) paired results that were concordant. The most common discordant viruses were rhinovirus/enterovirus, respiratory syncytial virus, and adenovirus. Mean relative viral loads were lower in discordant specimens compared to concordant specimens, regardless of specimen source.</div></div><div><h3>Conclusion</h3><div>Comparison of clinical and research specimens revealed that respiratory viral detection was similar with or without an added TS. Lower relative viral loads of discordant specimens suggest that a combined TS&MTS may not improve viral detection for clinically significant pathogens.</div></div>","PeriodicalId":15517,"journal":{"name":"Journal of Clinical Virology","volume":"178 ","pages":"Article 105801"},"PeriodicalIF":4.0000,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison of mid-turbinate nasal and combined nasal-throat specimen types for detection of respiratory viruses in children\",\"authors\":\"Irem Onalan , Zheyi Teoh , Sarah L. Steele , Eileen J. Klein , Bonnie Strelitz , Kirsten Lacombe , Erin M. Sullivan , Arun K. Nalla , Danielle M. Zerr , Janet A. Englund\",\"doi\":\"10.1016/j.jcv.2025.105801\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>The source of respiratory specimens may impact the detection of respiratory viruses. It may also have implications for research participant recruitment, caregiver acceptability due to concerns for patient comfort, and potential risk of aerosolization during epidemics/pandemics.</div></div><div><h3>Objective</h3><div>To determine the impact of collecting a throat swab (TS) in addition to a mid-turbinate nasal swab (MTS) by comparing agreement of viral detection and relative viral loads.</div></div><div><h3>Study design</h3><div>We reviewed molecular detection results from 2548 children enrolled in the New Vaccine Surveillance Network at Seattle Children’s Hospital from 11/2015–05/2019. Participants with a clinical MTS who agreed to collection of a combined TS and MTS (TS&MTS) for research were included. All specimens were tested using FilmArray<sup>R</sup> Respiratory Panel (Biofire Diagnostics). Viral detection from MTS and TS&MTS were compared. Relative viral loads were compared between specimens with concordant (same viruses detected) and discordant (different or additional viruses detected) results.</div></div><div><h3>Results</h3><div>Results from 743 participants with clinical MTS and research TS&MTS specimens were compared; Viral detections were similar between the two groups, including 596 (80.2 %) paired results that were concordant. The most common discordant viruses were rhinovirus/enterovirus, respiratory syncytial virus, and adenovirus. Mean relative viral loads were lower in discordant specimens compared to concordant specimens, regardless of specimen source.</div></div><div><h3>Conclusion</h3><div>Comparison of clinical and research specimens revealed that respiratory viral detection was similar with or without an added TS. Lower relative viral loads of discordant specimens suggest that a combined TS&MTS may not improve viral detection for clinically significant pathogens.</div></div>\",\"PeriodicalId\":15517,\"journal\":{\"name\":\"Journal of Clinical Virology\",\"volume\":\"178 \",\"pages\":\"Article 105801\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2025-04-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Clinical Virology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1386653225000435\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VIROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Clinical Virology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1386653225000435","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VIROLOGY","Score":null,"Total":0}

引用次数: 0

摘要

呼吸道标本的来源可能影响呼吸道病毒的检测。它还可能对研究参与者的招募、护理人员对患者舒适度的接受程度以及流行病/大流行期间雾化的潜在风险产生影响。目的通过比较病毒检测结果与相对病毒载量的一致性，确定除中鼻甲鼻拭子外还采集咽拭子（TS）的影响。我们回顾了2015年11月至2019年5月在西雅图儿童医院新疫苗监测网络中登记的2548名儿童的分子检测结果。患有临床MTS的参与者同意收集TS和MTS （TS&；MTS）联合用于研究。所有标本均使用FilmArrayR呼吸面板（Biofire Diagnostics）进行检测。比较MTS和TS&；MTS的病毒检测结果。比较结果一致（检测到相同病毒）和不一致（检测到不同或额外的病毒）的标本之间的相对病毒载量。结果对743例临床MTS和研究MTS标本的结果进行了比较；两组之间的病毒检测结果相似，其中596（80.2%）对结果一致。最常见的不一致病毒是鼻病毒/肠病毒、呼吸道合胞病毒和腺病毒。不一致标本的平均相对病毒载量低于一致标本，无论标本来源如何。结论临床标本与研究标本的比较表明，添加或不添加TS的呼吸道病毒检出率相似，不一致标本的相对病毒载量较低，表明联合使用TS和MTS可能不能提高对临床重要病原体的病毒检出率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Comparison of mid-turbinate nasal and combined nasal-throat specimen types for detection of respiratory viruses in children

Background

The source of respiratory specimens may impact the detection of respiratory viruses. It may also have implications for research participant recruitment, caregiver acceptability due to concerns for patient comfort, and potential risk of aerosolization during epidemics/pandemics.

Objective

To determine the impact of collecting a throat swab (TS) in addition to a mid-turbinate nasal swab (MTS) by comparing agreement of viral detection and relative viral loads.

Study design

We reviewed molecular detection results from 2548 children enrolled in the New Vaccine Surveillance Network at Seattle Children’s Hospital from 11/2015–05/2019. Participants with a clinical MTS who agreed to collection of a combined TS and MTS (TS&MTS) for research were included. All specimens were tested using FilmArray^R Respiratory Panel (Biofire Diagnostics). Viral detection from MTS and TS&MTS were compared. Relative viral loads were compared between specimens with concordant (same viruses detected) and discordant (different or additional viruses detected) results.

Results

Results from 743 participants with clinical MTS and research TS&MTS specimens were compared; Viral detections were similar between the two groups, including 596 (80.2 %) paired results that were concordant. The most common discordant viruses were rhinovirus/enterovirus, respiratory syncytial virus, and adenovirus. Mean relative viral loads were lower in discordant specimens compared to concordant specimens, regardless of specimen source.

Conclusion

Comparison of clinical and research specimens revealed that respiratory viral detection was similar with or without an added TS. Lower relative viral loads of discordant specimens suggest that a combined TS&MTS may not improve viral detection for clinically significant pathogens.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of Clinical Virology 医学-病毒学

CiteScore

22.70

自引率

1.10%

发文量

149

审稿时长

24 days

期刊介绍： The Journal of Clinical Virology, an esteemed international publication, serves as the official journal for both the Pan American Society for Clinical Virology and The European Society for Clinical Virology. Dedicated to advancing the understanding of human virology in clinical settings, the Journal of Clinical Virology focuses on disseminating research papers and reviews pertaining to the clinical aspects of virology. Its scope encompasses articles discussing diagnostic methodologies and virus-induced clinical conditions, with an emphasis on practicality and relevance to clinical practice. The journal publishes on topics that include: • new diagnostic technologies • nucleic acid amplification and serologic testing • targeted and metagenomic next-generation sequencing • emerging pandemic viral threats • respiratory viruses • transplant viruses • chronic viral infections • cancer-associated viruses • gastrointestinal viruses • central nervous system viruses • one health (excludes animal health)