Journal of Biologically Active Products from Nature最新文献

{"title":"Antimicrobial, Cytotoxic, and α-Glucosidase Inhibitory Potentials Using the One Strain Many Compounds Technique for Red Sea Soft Corals Associated Fungi’ Secondary Metabolites and Chemical Composition Correlations","authors":"Zeinab A. El-Shahid, Faten K Abd El-Hady, W. Fayad, M. Abdel‐Aziz, Eman M. Abd EL-Azeem, Emad K Ahmed","doi":"10.1080/22311866.2021.1978862","DOIUrl":"https://doi.org/10.1080/22311866.2021.1978862","url":null,"abstract":"Abstract Aspergillus unguis SPMD-EGY (A. unguis) and Aspergillus sp. 2C1-EGY (Aspergillus sp.) were isolated from the soft corals Sinularia and Lobophyton sp in the Hurghada coast, Red Sea, Egypt. One strain many compounds (OSMAC) was applied to trigger fungal silent biosynthetic genes. The results revealed that the A. unguis static filtrate extracts (St F) of all tested media exhibited the highest antimicrobial activity (inhibition zones 18.5-30.5 mm) against S. aureus, P. aeruginosa, and C. albicans. The A. unguis shake cell extracts of media A and C exhibited the highest α-glucosidase inhibitory activity (Sh Cell, 78 and 82 %). A high degree of acetylcholinesterase enzyme inhibition was obtained from A. unguis (Sh F, 61-66 %) shake filtrate extracts. The A. unguis extracts (A St F and D St Cell) demonstrated the highest cytotoxic activity against cancer cells, namely, HepG2, MCF-7, and HCT-116 (%I: 62-95; IC50: 7.9-90.8 µg/mL). The A. unguis extracts (A St F&D St F) exhibited high cytotoxic activities against HCT116 cancer spheroids compared with cisplatin (42.2 % and 51 %, respectively, vs 32.6 % for cisplatin). The biological activities of the extracts were due to the presence of various secondary metabolites detected by GC/MS analysis. Further pharmacological studies may lead to the isolation of new antitumor compounds besides the prospective antimicrobial and α-glucosidase inhibition capacity of the active extracts. Graphical abstract","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41631092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potentiation of the Antibiotic Activity by the Essential Oils of Eugenia brasiliensis Lam. and Piper mosenii C. DC.","authors":"Rakel Olinda Macedo da Silva, M. K. D. N. Silva Leandro, A. C. Araújo, José Walber Gonçalves Castro, Orlando de Menezes Dantas Junior, Raíra Justino Oliveira Costa, Luciely Leite Pinto, Jaime Ribeiro-Fil-ho, L. E. da Silva, W. Amaral, C. Deschamps, E. F. Matias, H. Coutinho","doi":"10.1080/22311866.2021.1987322","DOIUrl":"https://doi.org/10.1080/22311866.2021.1987322","url":null,"abstract":"Abstract The present study aimed to investigate the antibiotic-enhancing activity of the essential oils obtained from the leaves of Eugenia brasiliensis Lam (EOEb) and Piper mosenii C. DC. (EOPm) against multiresistant strains of Escherichia coli 06 and Staphylococcus aureus 10. The essential oils were obtained by hydrodistillation, and the minimum inhibitory concentrations (MIC) were determined through the broth microdilution method. The EOEb enhanced the activities of norfloxacin and erythromycin against Staphylococcus aureus and Escherichia coli, respectively. At the same conditions, the EOPm was found to potentiate the effects of gentamicin and erythromycin against E. coli. Of note, this is the first study to date that has reported the ability of these extracts to enhance the antibacterial activity of conventional antibiotics.","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42925598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolite Profiling of Peltophorum africanum Sond. & Saraca indica L. Leaves via HR-UPLC/PDA/ESI/MS Analysis and Assessment of their Anti-Diabetic Potential","authors":"A. A. Abou Zeid, S. E. El Hawary, R. Mohammed, W. Ashour, Kawkab A. Ahmed, O. Sabry, H. Attia","doi":"10.1080/22311866.2021.1943523","DOIUrl":"https://doi.org/10.1080/22311866.2021.1943523","url":null,"abstract":"Abstract This study aimed to screen different extracts (E) such as total ethanol (TE), petroleum ether (PE) chloroform (CH), ethyl acetate (EA) and methanol (AM) extracts of Peltophorum africanum Sond. (PA) and Saraca indica L. (SI) leaves (family fabaceae) for their in-vitro antidiabetic activity using α-amylase inhibition assay. The extracts that showed remarkable in vitro activity were further subjected to in vivo anti-diabetic assessment using streptozotocin-induced diabetes model in experimental animals where serum glucose, C-peptide, liver glycogen content in addition to lipid peroxide level in both serum and liver were estimated biochemically in normal and diseased rats. The average percent of weight change and kidney to body weight ratio percent were calculated for all animal groups. Histopathological examination of pancreas, liver and kidney sections isolated from all animals was carried out. UPLC/MS/MS analysis of TEE of PA and SI for chemical investigation was performed. Results of the in vitro anti diabetic activity revealed that EAE, PEE& TEE of PA and TEE & EAE of SI possessed the highest inhibitory activity as compared to acarbose. In vivo antidiabetic activity of the selected extracts revealed that animal groups treated daily with EAE of PA and SI exhibited a significant improvement in carbohydrate metabolism and lipid peroxide level, with superior effect in comparison with that of TEE and PEE of both plants. Histopathological findings showed marked protection particularly in sections isolated from animals treated with EAE of PA. UPLC/MS/MS analysis of TEE of both plants identified 46 metabolites belonging to various classes. It could be suggested that active extracts of both plants could be considered as potential natural antioxidant and anti-diabetic agents.","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44413566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid in vitro Leaf Biomass Production of Genetically Stable Curcuma aromatica- An Under Exploited Medicinal Plant","authors":"Reena Parida, S. Nayak","doi":"10.1080/22311866.2021.1970022","DOIUrl":"https://doi.org/10.1080/22311866.2021.1970022","url":null,"abstract":"Abstract Surface sterilized dormant axillary buds of rhizome of Curcuma aromatica Salisb., were cultured on Murashige and Skoog (MS) with benzyladenine (BA), kinetin (KIN), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), naphthalene acetic acid (NAA) and adenine sulphate (Ads) in different concentrations and combinations. Activation of explants occurred after 25 days of culture. The highest shoot numbers per explant ((7.4±0.3) was recorded on medium with 1 mg/l of BA and 0.5 mg/l of KIN. The maximum leaf biomass production was on 3 mg/l of BA and 0.5 mg/l of IAA (12.1±0.3 gm per plant). Similarly, the rooting was also observed maximum per plant (5.1±0.5) on 1 mg/l of BA and 0.5 mg/l of IBA. Two months old plantlets were transferred to fresh MS basal media for further growth. The micro propagated plants were acclimatized in greenhouse and after six months their survival rate were recorded as 95 % respectively. The genetic stability and fidelity of micropropagated plants was assessed through analysis of inter simple sequence repeats as compared to the mother plant. Nine ISSR primers produced a total number of 3350 bands and all were monomorphic. No variations among the micropropagated plants were recorded for two generations. The high antioxidant since present in the leaves of this plant enables us to produce more number of leaf biomass to meet the demand of aromatic leaf essential oil. Thus the process can be used for conservation and mass propagation of true-to-type Curcuma aromatica. Graphical abstract","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41822092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactivity and Functional Properties of Protein Hydrolysate from Muscle and Visceral Waste of Ribbon Fish (Lepturacanthus savala) Extracted by Three Different Proteolytic Enzymes","authors":"Undiganalu Gangadharappa Yathisha, I. Karunasagar, Mamatha Bs","doi":"10.1080/22311866.2021.1953399","DOIUrl":"https://doi.org/10.1080/22311866.2021.1953399","url":null,"abstract":"Abstract The study focuses on utilization of solid visceral waste from ribbon fish in preparation of protein hydrolysates, possessing bioactive properties. Three different proteolytic enzymes (alcalase 2.4 U/g, flavourzyme 500 U/g, and papain 1 U/mL) were used to standardize the preparation of visceral waste protein hydrolysates (VPH) in comparison to muscle protein hydrolysate (MPH). Based on the preliminary studies, the hydrolysis process was standardized to 4 h at 1.5 % (w/v) enzyme concentration. MPH and VPH form all the enzymes were taken to study the bioactive (ACE-1 and antioxidant activity) and functional properties. MPH and VPH extracted from alcalase (IC50 = 0.835 and 0.902 mg/mL) showed higher angiotensin converting enzyme-1 (ACE-1) inhibitory activity than flavourzyme (IC50 = 1.602 and 1.323 mg/mL) and papain (1.263 and 1.565 mg/mL). Similarly, alcalase extracted samples were showed higher levels of radical scavenging activity (IC50 = 2.538 and 2.835 mg/mL) than papain (IC50 = 8.382 and 9.389 mg/mL) and flavourzyme (IC50 = 10.562 and 11.56 mg/mL). The present study proves that visceral waste, which is often discarded in a larger quantity, polluting the environment can be converted into a nutraceutical component with greater health benefits.","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43490699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Food-Derived Bioactive Peptides: A Promising Substitute to Chemosynthetic Drugs Against the Dysregulated Renin-Angiotensin System in COVID-19 Patients","authors":"A. Mahamud, Md Ehsanul Kabir, A. Sohag, Chong Chen, M. Hannan, M. H. Sikder, Keshab Bhattarai, B. Baral, Md Jamal Uddin","doi":"10.1080/22311866.2021.1945494","DOIUrl":"https://doi.org/10.1080/22311866.2021.1945494","url":null,"abstract":"Abstract Coronavirus disease-19 (COVID-19) pandemic is a global threat caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). The viral infection dysregulates the functions of the renin-angiotensin system (RAS) through an interaction between SARS-CoV-2 spike protein and angiotensin-converting enzyme 2 (ACE2), leading to an upregulated level of Angiotensin II (Ang II) in blood plasma. The enhanced level of Ang II may contribute to various pathophysiological events, including vasoconstriction, oxidative stress, endothelial dysfunction, inflammation, beta-cell dysfunction, and many others. These phenomena are associated with developing multiple chronic diseases, including hypertension, cardiovascular disease, diabetes, lung disease, renal disease, and many other comorbidities in COVID-19 patients. Thus, the SARS-CoV-2 infection may contribute to severe conditions and higher mortality in COVID-19 patients with underlying comorbidities. Several synthetic drugs, especially RAS blockades, are currently prescribed to COVID-19 patients to minimize the severity of these comorbidities by limiting the deleterious effects of Ang II. However, these chemosynthetic drugs are limited by several side effects such as persistent cough, fetal abnormalities, hepatic disorder as well as promotion in the occurrence of new chronic diseases. These drawbacks raise an investigation to explore comparatively safe alternatives for COVID-19 patients. From this point of view, we have anticipated that applications of multifunctional food-derived bioactive peptides could be a promising approach through their preventive and therapeutic actions against underlying chronic complications in COVID-19 patients. This review enlightened the disease preventive and immunomodulatory effects of food-derived bioactive peptides that may enhance the survivability and vitality of COVID-19 patients with chronic complications.","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/22311866.2021.1945494","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41444492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postprandial Glycaemic Effect of a Peptide Fraction of Salvia Hispanica in Patients with Insulin Resistance","authors":"Irving F. Sosa-Crespo, Alan Espinosa-Marrón, L. Chel-Guerrero, Hugo Lavia-da-Molina, D. Betancur-Ancona","doi":"10.1080/22311866.2021.1942987","DOIUrl":"https://doi.org/10.1080/22311866.2021.1942987","url":null,"abstract":"Abstract Diabetes is a prevalent metabolic disorder among almost every single population on the planet. Diet has a fundamental impact on both treatment and control. The consumption of several plant seeds has been associated with improvement in glucose tolerance and absorption. A randomized controlled trial was conducted to evaluate the glycaemic effects of a hydrolyzed peptide fraction of Salvia hispanica seed through postprandial quantification on blood glucose, plasma insulin, and Matsuda-DeFronzo index in patients with insulin resistance or incipient type 2 diabetes. Both Chia and control proteins were mixed with a portion of liquid food and provided to nine participants. A mixed-food tolerance curve was performed. A reduction in glucose concentrations was observed after 30 minutes in subjects consuming Chia seeds' peptides. However, no significant effect on plasmatic insulin was found. The consumption of peptide fractions of Chia seeds could decrease the acute absorption of carbohydrates and attenuate acute postprandial hyperglycemia in patients with T2D. However, these apparent benefits do not appear to modulate insulin sensitivity.","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/22311866.2021.1942987","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42570932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro Antioxidant and Anticholinesterase Activities of Senecio massaicus Essential Oil and Its Molecular Docking Studies as a Potential Inhibitor of Covid-19 and Alzheimer’s Diseases","authors":"Sara Kebbi, Labib Noman, I. Demirtaş, C. Bensouici, Ş. Adem, S. Benayache, F. Benayache, R. Seghiri, Mesut Gok","doi":"10.1080/22311866.2021.1955006","DOIUrl":"https://doi.org/10.1080/22311866.2021.1955006","url":null,"abstract":"Abstract The composition of the essential oil obtained from the dried aerial parts of Senecio massaicus was analyzed by GC/MS. Twenty-two components have been identified and represented 97.41 % of the total oil composition. The major constituents of the essential oil were m-cymene (30.58 %), n-hexadecanoic acid (14.88 %) and docosane-11-decyl (10.43 %). Four methods were used to determine the antioxidant activity: DPPH, ABTS, CUPRAC and reducing power assay. The results indicate that the essential oil extract has moderate to low activity compared to the reference antioxidant compounds. In vitro anticholinesterase activity of the essential oil has also been studied. It exhibited higher inhibitory activity against butyrylcholinesterase (BChE) than against acetylcholinesterase (AChE). Docking studies conducted for Alzheimer's disease-related enzymes have displayed that compounds docosane-11-decyl and octaethyleneglycol monododecyl ether have strong potency, and compounds 15,15’Bi1,4,7,10,13-pentaoxacyclohexadecane and n-Hexadecanoic acid have moderate inhibitory potential. In addition, these three compounds (Docosane-11-decyl, octaethyleneglycol monododecyl ether and 15,15’Bi1,4,7,10,13-pentaoxacyclohexadecane) of the essential oil displayed strong interaction against SARS-CoV-2 main protease and Nsp15 endoribonuclease. Therefore, it could be useful to provide anticholinesterase agent and anti-coronavirus candidate drugs.","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44357352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxic Phytochemicals from Myrsinaceae Family and their Modes of Action: A Review","authors":"Musthahimah Muhamad, C. Choo, C. Leow","doi":"10.1080/22311866.2021.1945493","DOIUrl":"https://doi.org/10.1080/22311866.2021.1945493","url":null,"abstract":"Abstract Cancer is one of the leading causes of illness and death worldwide, with approximately 19.3 million new cases and 10.0 million cancer deaths worldwide. Natural products have provided diverse classes of compounds with potent antitumor activity. Species from the Myrsinaceae family are commonly used as traditional medicine worldwide, namely, China, East Asia, Africa, south-east Asia and Latin America to treat a variety of diseases. The cytotoxic phytochemicals from species of this family included alkylresorcinols and triterpene saponins. The objective of this review is to summarize the cytotoxic phytochemicals isolated from the Myrsinaceae family, their mode of action and structural requirements for their cytotoxic activities. This review was based on information collected from the ScienceDirect, Scopus, Chemical and Pharmaceutical Bulletin and American Chemistry Society databases from January 1987 to January 2021. A total of 192 alkylresorcinols and triterpenoid saponins were isolated from the Myrsinaceae family and most exhibited cytotoxic activity with IC50 values between 0.5 to <100 µM. The alkylresorcinols (12 and 15) and triterpenoid saponins (104 and 107) exhibited IC50 values comparable to the standard drugs. Only five compounds were evaluated on their mechanism of action. Cell death was related to activation of inhibitor caspase 8, inhibition of vascular endothelial growth factor (VEGF) and suppression of the ubiquitin fusion degradable protein expression. Both the alkylresorcinols and triterpenoid saponins have the potential to be evaluated further for possible multi-targeted cancer agents. Graphical abstract","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41383502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Investigation and HPTLC Fingerprinting Analysis of Selaginella bryopteris Ethanolic Plant Extract for Analgesic and Anti-inflammatory Activities in Animals","authors":"S. Paswan, Pritt Verma, L. Azmi, Sajal Srivastava, Chandana Venkateswara Rao","doi":"10.1080/22311866.2021.1918023","DOIUrl":"https://doi.org/10.1080/22311866.2021.1918023","url":null,"abstract":"Abstract The present study was performed to determine analgesia and anti-inflammatory activities of whole plant extract of Selaginella bryopteris on animals. The extract of S. bryopteris whole plant was performed using ethanol (70%) as a solvent. The phytochemical investigation of plant extract was done to evaluated secondary metabolites present in the plant. It was further characterized using HPTLC technique to identify active constituents present in the S. bryopteris extract. The efficacy of plant extract was evaluated using the hot plate method and tail flick method against pain and carrageenan-induced paw oedema model against inflammation. Results exhibited that S. bryopteris extract possesses alkaloids, glycosides, tannins, saponins, flavonoids, carbohydrate, protein and amino acids, steroids, vitamins, gums and mucilages. HPTLC fingerprinting analysis revealed the presence of gallic acid and rutin as active constituents present in the plant extract. S. bryopteris extract showed significant analgesic and anti-inflammatory activities. Thus, it can be used as a safer alternative drug against pain and inflammation in comparison to existing non-steroidal anti-inflammatory drugs (NSAIDs).","PeriodicalId":15364,"journal":{"name":"Journal of Biologically Active Products from Nature","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46303060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}