Journal of Chinese medicinal materials Pub Date : 2016-07-01

Jian-hai Ding, Tao Feng, Zheng-hui Li, Ji-kai Liu

引用次数: 0

[Simultaneous Determination of Protocatechuic Acid,Kaempferol Biotin Glucoside and Quercitrin in Rat Plasma and Pharmacokinetics By UPLC-MS/MS]. [UPLC-MS/MS同时测定大鼠血浆中原儿茶酸、山奈酚生物素、葡萄糖苷和槲皮苷的药代动力学]。

Journal of Chinese medicinal materials Pub Date : 2016-07-01

Lin Zheng, Wu Yang, Wen-ying Xiang, Jia Sun, Si-ying Chen, Yong-jun Li

{"title":"[Simultaneous Determination of Protocatechuic Acid,Kaempferol Biotin Glucoside and Quercitrin in Rat Plasma and Pharmacokinetics By UPLC-MS/MS].","authors":"Lin Zheng, Wu Yang, Wen-ying Xiang, Jia Sun, Si-ying Chen, Yong-jun Li","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To develop a sensitive and reliable ultra performance liquid chromatography tandem mass spectrometry ( UPLC-MS / MS) method for simultaneous determination and pharmacokinetics of protocatechuic acid, kaempferol biotin glucoside and quercitrin in rat plasma, and study their pharmacokinetic characteristics in rats.Methods: Three compounds were simultaneously determined by UPLC-MS / MS on Waters BEH C18( 50 mm × 2. 1 mm,1. 7 μm) column. The mobile phase was 0. 1% acetonitrile formic acid and0. 1% aqueous formic acid, and programmed in a linear gradient mode. The compounds were ionized in the electrospray ionization ion source of the mass spectrometer and detected in MRM mode.Results: The t1 /2of protocatechuic acid,kaempferol biotin glucoside and quercitrin after intravenous injection were( 41. 9 ± 12. 3),( 71. 3 ± 56. 8) and( 90. 3 ± 74. 8) min. The Cmaxwere( 1. 7 ± 0. 7) μg / m L,( 2. 1 ± 0. 9) μg/m L,( 8. 7 ± 3. 7) μg/m L.Conclusion: The established method is specific,rapid,accurate and sensitive,and is proved to meet the requirements of biological sample analyses,and is suitable for the concentration determination of protocatechuic acid, kaempferol biotin glucoside and quercitrin in rat plasma, three compounds are all best fitted to the two-compartment open pharmacokinetic model.","PeriodicalId":15312,"journal":{"name":"Journal of Chinese medicinal materials","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36479476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Mechanism Research of Gambogenic Acid on Proliferation,Apoptosis and Invasion of Cervical Carcinoma He La Cells]. [藤黄酸对宫颈癌He La细胞增殖、凋亡及侵袭的影响机制研究]。

Journal of Chinese medicinal materials Pub Date : 2016-06-01

Yu-rong Shi, Ying Yang

{"title":"[Mechanism Research of Gambogenic Acid on Proliferation,Apoptosis and Invasion of Cervical Carcinoma He La Cells].","authors":"Yu-rong Shi, Ying Yang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the effects of gambogenic acid on proliferation,apoptosis and invasion of human cervical carcinoma HeLa cells.Methods: HeLa cells were given different concentrations of gambogenic acid( 0. 00,0. 63,1. 25,2. 50,5. 00 and 10. 00 mg / L) for 72 h,MTT assay was used to measure the cells proliferation. Flow cytometry was used to measure cell cycle. Fluorescence microscopy and DNA ladder analysis were used to measure the apoptosis of HeLa cells. Transwell chambers was adopted to detect the cells invasion. The expression levels of mRNA and protein of BCL-2,BAX,NF-κB and E-cadherin were detected by RT-PCR and Western blot.Results: After treatment with different concetrations of gambogenic acid for 72 h, the proliferation of HeLa cells was significantly inhibited, and in a concentration dependent manner. The ability of invasion was decreased with the concentration of gambogenic acid increased, which was detected by Transwell chamber assays in vitro. RT PCR and Western blot demonstrated that gambogenic acid up-regulated the expressions of BAX and E-cadherin and down-regulated the expression of mRNA and protein of BCL-2 and NF-κB.Conclusion: Gambogenic acid can inhibite the proliferation and invasion of HeLa cells,and promote the cells apoptosis in a concentration dependent manner.","PeriodicalId":15312,"journal":{"name":"Journal of Chinese medicinal materials","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36437601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Study on Material Base of Panax ginseng for Treating Brain Ischemia Based on Molecular Docking]. [基于分子对接的人参治疗脑缺血物质基础研究]。

Journal of Chinese medicinal materials Pub Date : 2016-06-01

Gui-zhu Deng, Chun-wei Wu, Jie-xin Lin, Chao Cheng, Shu-mei Wang

{"title":"[Study on Material Base of Panax ginseng for Treating Brain Ischemia Based on Molecular Docking].","authors":"Gui-zhu Deng, Chun-wei Wu, Jie-xin Lin, Chao Cheng, Shu-mei Wang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: On the basis of molecular docking,to study the mechanism of Panax ginseng in the treatment of ischemic stroke.Methods: The small molecules of Panax ginseng based on molecular docking technology docked with 20 key targets protein of cerebral ischemia, and multi-component protein target network was established by Cytoscape 3. 1. 1 software. At the same time,the two active molecules of the Panax ginseng of Rb2 and 20( R)-ginsenoside Rg2 were also analyzed by VEGF and Caspase-3,which were the key protein in brain ischemia.Results: There were 31 active molecules of Panax ginseng combined strongly with five or more than five protein targets after molecular docking, and only four active molecules of Panax ginseng combined strongly with ten or more than ten protein targets. The two active molecules of Rb2 and 20( R)-ginsenoside Rg2 in the Panax ginseng had a very strong combination with VEGF and Caspase-3,respectively,and the docking scores were more than 7. 0.Conclusion: Molecular docking technology screening active substances of Panax ginseng plays a practical significance in the treatment of ischemic stroke, which offers the foundation to study the chemical constituents of composite prescription.","PeriodicalId":15312,"journal":{"name":"Journal of Chinese medicinal materials","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36439811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Optimization on Preparation of Total Saponins of Aralia taibaiensis Phospholipid Complex by Central Composite Design-Response Surface Method]. [中心设计-响应面法优化太白楤木磷脂复合物总皂苷的制备]。

Journal of Chinese medicinal materials Pub Date : 2016-06-01

Bing-tao Zhai, Jia Li, Xiao-li Liu, Wei-ping Yang, Bei Zheng, Ya-jun Shi, Dong-yan Guo

{"title":"[Optimization on Preparation of Total Saponins of Aralia taibaiensis Phospholipid Complex by Central Composite Design-Response Surface Method].","authors":"Bing-tao Zhai, Jia Li, Xiao-li Liu, Wei-ping Yang, Bei Zheng, Ya-jun Shi, Dong-yan Guo","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To optimize the preparation of total saponins of Aralia taibaiensis phospholipid complex( TSAT-PC) by the central composite design-response surface method.Methods: Total saponins of Aralia taibaiensis phospholipid complex was prepared by using solvent evaporation method, five factors including reaction solvent, reaction time, reaction temperature, ratio of reactants on this reaction, and the concentration of the drug were investigated, then to optimize the preparation of TSAT-PC by the central composite design response surface method, and to study its physicochemical properties.Results: The optimal process conditions were as follows, the reaction time was 1 h, the reaction temperature was 45 ℃,the ratio of soya lecithine ( SL) and TSAT was 3∶ 1, the reaction concentration was16 mg / m L, the complexing rate was 97. 23%,it was less than 5% with the predicted deviation; IR analysis proved the formation of TSAT-PC, and the solubility in the octyl alcohol was higher than the original drug.Conclusion: TSAT-PC was successfully developed by the optimized process, enhance the solubility in octyl alcohol, which provide the reference for the further development and utilization of Chinese materia medica preparation.","PeriodicalId":15312,"journal":{"name":"Journal of Chinese medicinal materials","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36437596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Correlation Analysis of Curcuminoids and Color Index L* , a* and b* of Curcumae Longae Rhizoma Powder]. [姜黄根粉中姜黄素与颜色指数L*、a*、b*的相关性分析]。

Journal of Chinese medicinal materials Pub Date : 2016-06-01

Li-li Yin, Qin Xia, Xiao-yang Cai, Min Li

{"title":"[Correlation Analysis of Curcuminoids and Color Index L* , a* and b* of Curcumae Longae Rhizoma Powder].","authors":"Li-li Yin, Qin Xia, Xiao-yang Cai, Min Li","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To study the relationship between the component content of curcuminoids in Curcumae Longae Rhizoma and powder color indeces L*, a* and b* those were measured by chromaticity instrument, in order to provide scientific basis for quality assessment of Curcuma longa medicinal materials.Methods: Detect the content of curcumin,demethoxycurcumin,bisdemethoxycurcumin,then detect the content of curcuminoids by the Chinese Pharmacopoeia method. Measure the color indeces L*, a* and b* of Curcumae Longae Rhizoma powder by colorimeter. Finally, the relationship between the content and the color was analyzed by using the Grey Relational Analysis, Pearson correlation coefficient and regression analysis.Results: There was positive correlation between the content of curcuminoids and a*. But there was no definitely relation between the content of curcuminoids and b* or L*.Conclusion: The content of curcuminoids is closely related with the degree of the color red, the higher the content, the red is deeper. a* could be recognized as an important basis of Curcumae Longae Rhizoma medicinal materials quality.","PeriodicalId":15312,"journal":{"name":"Journal of Chinese medicinal materials","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36439292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Effect of Jiawei Yinchen Sini Decoction on the Production of Smad7 and CollagenⅠ,Ⅲ in Carbon Tetrachloride Induced Hepatic Fibrosis Model of Mice]. [加味银臣四逆汤对四氯化碳致小鼠肝纤维化模型中Smad7及胶原蛋白Ⅰ，Ⅲ生成的影响]。

Journal of Chinese medicinal materials Pub Date : 2016-06-01

Jie Li, Jian-li Qiu, Hua Xu

{"title":"[Effect of Jiawei Yinchen Sini Decoction on the Production of Smad7 and CollagenⅠ,Ⅲ in Carbon Tetrachloride Induced Hepatic Fibrosis Model of Mice].","authors":"Jie Li, Jian-li Qiu, Hua Xu","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To explore the preventive effects of Jiawei Yinchen Sini Decoction on the gene expression of Smad7 and Collagen,Ⅲ in mice with hepatic fibrosis.Methods: 48 male ICR mice were randomly divided into normal group, model group, colchicine group, JWYSN high dose group, medium dose group and low dose group, with 8 mice in each group. The hepatic fibrosis model was established by intraperitoneal injection of 30% CCl4( 1. 5 mg/kg, dissolved in olive oil). At the same time, the mice were treated with intragastric adminstration of drugs. After 14 days, the liver index was calculated; the levels of serum hyaluronic acid( HA) and laminin protein( LN) were detected by Enyme-linked immunosorbent assay; the pathological fibrosis change was observed by performing HE staining and was made a grading of hepatic fibrosis. mRNA expression of Smad7 and Collagen I, Ⅲ were detected by RT-q PCR analysis.Results: (1) Compared with model group, the liver index and levels of HA and LN in JWYSN medium dose group and low dose group were obviously decreased( P < 0. 05).( 2) The pathological results showed that, compared with model group, the hepatic fibrosis were significantly relief in different dose of JWYSN group( P < 0. 05).( 3) The expressions of Collagen Ⅰ mRNA in each treatment group were significantly lower than model group( P < 0. 05); In addition to JWYSN high dose group, the expressions of Smad7 mRNA in each treatment group increased significantly( P < 0. 05); In addition to JWYSN low dose group, the expression of CollagenⅢmRNA in each treatment group decreased significantly( P < 0. 05).Conclusion: Jiawei Yinchen Sini decoction can up-regulate the expressions of Smad7 mRNA and inhibit expressions of mRNA and CollagenⅢmRNA in mice with hepatic fibrosis,thus attenuate the development of hepatic fibrosis.","PeriodicalId":15312,"journal":{"name":"Journal of Chinese medicinal materials","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36437600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Study on the Chemical Constituents of Litchi chinensis Pericarp( Ⅱ)]. [荔枝果皮化学成分研究[Ⅱ]]。

Journal of Chinese medicinal materials Pub Date : 2016-06-01

Xiao-li Guan, Yong-lin Huang, Ya-feng Wang, Dian-peng Li

引用次数: 0

[Study on Flavanoids Constituents of Swertia binchuanensis]. 【滨川獐牙菜黄酮类成分研究】。

Journal of Chinese medicinal materials Pub Date : 2016-06-01

Zhao-yun Li, Ying Wang, Yun-jiao Guo, Long-xing Li, Huai Xiao

引用次数: 0

[Chemical Constituents from Stems of Kadsura longipedunculata]. [长柄角菜茎的化学成分]。

Journal of Chinese medicinal materials Pub Date : 2016-06-01

Yao-jie Guo, Shi-man Gao, Ben-gang Zhang, Hai-tao Liu

引用次数: 0

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