[UPLC-MS/MS同时测定大鼠血浆中原儿茶酸、山奈酚生物素、葡萄糖苷和槲皮苷的药代动力学]。

中药材 Pub Date : 2016-07-01

Lin Zheng, Wu Yang, Wen-ying Xiang, Jia Sun, Si-ying Chen, Yong-jun Li

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引用次数: 0

摘要

目的:建立灵敏可靠的超高效液相色谱-串联质谱(UPLC-MS / MS)同时测定大鼠血浆中原儿茶酸、山奈酚生物素糖苷和槲皮苷的药动学方法，研究其在大鼠体内的药动学特征。方法:采用Waters BEH C18(50 mm × 2)高效液相色谱-串联质谱法(UPLC-MS / MS)同时测定3种化合物。1毫米,1。7 μm)柱。流动相为0。1%乙腈甲酸和0。1%甲酸水溶液，并以线性梯度模式编程。化合物在质谱仪的电喷雾电离离子源中电离，并在MRM模式下检测。结果:原儿茶酸、山奈酚生物素、葡萄糖苷、槲皮苷经静脉注射后的t1 /2为(41)。9±12。3),(71。3±56。8)和(90);3±74。8) min。7±0。7) μg / m L;1±0。9) μg/m L;7±3。结论:所建立的方法特异、快速、准确、灵敏，满足生物样品分析的要求，适用于大鼠血浆中原儿茶酸、山奈酚生物素、葡萄糖苷和槲皮苷的浓度测定，3种化合物均符合双室开放药代动力学模型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

本刊更多论文

[Simultaneous Determination of Protocatechuic Acid,Kaempferol Biotin Glucoside and Quercitrin in Rat Plasma and Pharmacokinetics By UPLC-MS/MS].

Objective: To develop a sensitive and reliable ultra performance liquid chromatography tandem mass spectrometry ( UPLC-MS / MS) method for simultaneous determination and pharmacokinetics of protocatechuic acid, kaempferol biotin glucoside and quercitrin in rat plasma, and study their pharmacokinetic characteristics in rats.

Methods: Three compounds were simultaneously determined by UPLC-MS / MS on Waters BEH C18( 50 mm × 2. 1 mm,1. 7 μm) column. The mobile phase was 0. 1% acetonitrile formic acid and0. 1% aqueous formic acid, and programmed in a linear gradient mode. The compounds were ionized in the electrospray ionization ion source of the mass spectrometer and detected in MRM mode.

Results: The t1 /2of protocatechuic acid,kaempferol biotin glucoside and quercitrin after intravenous injection were( 41. 9 ± 12. 3),( 71. 3 ± 56. 8) and( 90. 3 ± 74. 8) min. The Cmaxwere( 1. 7 ± 0. 7) μg / m L,( 2. 1 ± 0. 9) μg/m L,( 8. 7 ± 3. 7) μg/m L.

Conclusion: The established method is specific,rapid,accurate and sensitive,and is proved to meet the requirements of biological sample analyses,and is suitable for the concentration determination of protocatechuic acid, kaempferol biotin glucoside and quercitrin in rat plasma, three compounds are all best fitted to the two-compartment open pharmacokinetic model.

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来源期刊

中药材

自引率

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发文量

11364

期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.