Journal of Chinese medicinal materials Pub Date : 2016-06-01

Yu Zeng, Xing-fu Chen, Jie Meng, Jiu-hua Song, Wen-yu Yang, Tao Cheng

{"title":"[The Effection Study of Transplanting Techniques for Yield and Quality of Gentiana crassicaulis].","authors":"Yu Zeng, Xing-fu Chen, Jie Meng, Jiu-hua Song, Wen-yu Yang, Tao Cheng","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To study the difference in yield and quality of Gentiana crassicaulis with different transplanting period and transplanting methods, and to determine the optimum transplanting technique of Gentiana crassicaulis in Ludian.Methods: The variation in fresh weight,dry weight,dry discount rate, length, diameter, branches,the content of gentiopicroside,loganin acid,alcohol-soluble extract, total ash were measured, and made a comprehensive evaluation of yield and quality by gray relational distance ideal comprehensive evaluation method.Results: There was a big difference in yield and quality of Gentiana crassicaulis with different transplanting time and transplanting methods. Gentiana crassicaulis were transplanted in March 10 with the density of 25 cm × 24 cm,and overburden 1 cm and flat transplanting had the best comprehensive evaluation of yield and quality.Conclusion: The transplanting time of Gentiana crassicaulis in Ludian should begin at the end of early March,and reduce overburden soil on the traditional transplanting methods.","PeriodicalId":15312,"journal":{"name":"Journal of Chinese medicinal materials","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36437364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Chemical Constituents from Xanthium mongolicum]. [蒙古苍耳的化学成分]。

Journal of Chinese medicinal materials Pub Date : 2016-06-01

Xiao-mei Fu, de-hong Liu, Yan-chao Sun, Jing Liu, Jian-guo Pei, Zhi-gui Wu

引用次数: 0

[Study on HPLC Fingerprint of Solanum nigrum Fruit]. 龙葵果实HPLC指纹图谱研究

Journal of Chinese medicinal materials Pub Date : 2016-06-01

Peng-peng Dong, Quan-xi Mei, Fan Zhang

引用次数: 0

[Quality Evaluation of Rhei Radix et Rhizoma Based on SPE and HPLC Characteristic Chromatogram with An Internal Standard]. 基于内标固相萃取和高效液相色谱特征图谱的大黄药材质量评价

Journal of Chinese medicinal materials Pub Date : 2016-06-01

Liang Wang, Wei Guo, Qian Zhou, Jun Zhang, Zong-yuan Yu

{"title":"[Quality Evaluation of Rhei Radix et Rhizoma Based on SPE and HPLC Characteristic Chromatogram with An Internal Standard].","authors":"Liang Wang, Wei Guo, Qian Zhou, Jun Zhang, Zong-yuan Yu","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To establish a quality evaluation method for Rhei Radix et Rhizoma based on SPE and internal standard HPLC characteristic chromatogram.Methods: Using water reflux extraction and SPE methods, the total Rhei Radix et Rhizoma anthraquinones were obtained. By HPLC method, the characteristic chromatogram of Rhei Radix et Rhizoma was established,and the qualitative research was performed with DAD and MSn. Relative area of the characteristic peaks in the samples was calculated by the addition of an internal standard to evaluate the quality of Rhei Radix et Rhizoma.Results: The results showed that the optimum condition were as follows,the solid-liquid ratio was 1∶ 150; the extraction time was 60 min. SPE optimum condition for the introduction of Accu BOND Ⅱ SPE ODS-C18 Cartridges SPE were as follows, the loaded concentration was 1 m L, the SPE column was rinsed twice with 3 m L 0. 3% formic acid, then rinsed with 3 m L 25% methanol( containing 0. 3% formic acid) and finally eluted with 3 m L of methanol. The recovery rate was 96. 3% ~ 103%. With 5-hydroxymethylfurfural as an internal standard, the characteristic chromatogram was established, and twelve peaks were selected as characteristic peaks. In ten batches of samples, the RSD of relative retention time was < 3%,and the relative area of the characteristic peaks were calculated. The principal component analysis and cluster analysis with the results of the Chinese Pharmacopoeia( 2015 edition),which indicated that the characteristic chromatogram could reflect the anthraquinones in Rhei Radix et Rhizoma, and distinguish the Rheum palmatum, Rheum tanguticum and Rheum officinale.Conclusion: It is concluded that the established method is simple,fast and specific, which can provide a reference for the evaluation of the quality of Rhei Radix et Rhizoma.","PeriodicalId":15312,"journal":{"name":"Journal of Chinese medicinal materials","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36437595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[HPLC Analyzed the Iridoid Glycosides of Qingyedan Commonly Used in Folk Medicine in Yunnan]. 【高效液相色谱法分析云南民间医药常用青叶丹环烯醚萜苷】。

Journal of Chinese medicinal materials Pub Date : 2016-06-01

Hai-lan Fang, Fei-neng Shang, Xian-ying Yang, Cong-long Xia, Bao-zhong Duan

引用次数: 0

[Study on Regulation Effects of Yiqiyangyin Formula on Advanced Lung Cancer Cachexia Mice]. [益气养阴方对晚期肺癌恶病质小鼠调节作用研究]。

Journal of Chinese medicinal materials Pub Date : 2016-06-01

Bin Li, Yan-xiao Li, Hong-ran Pan

{"title":"[Study on Regulation Effects of Yiqiyangyin Formula on Advanced Lung Cancer Cachexia Mice].","authors":"Bin Li, Yan-xiao Li, Hong-ran Pan","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To observe the effect of Yiqiyangyin formula on advanced lung cancer cachexia mice.Methods: 60 C57 BL /6inbred mice( SPF) were randomly divided into normal control group, model group, Yiqiyangyin formula group,indomethacin group, and cisplatin group, with 12 mice in each group. Model group and normal control group were treated with the same amount of normal saline. Except for the normal control group, the mice of the other groups were built with Lewis lung cancer model. The changes of the level of body mass, feed consumption,serum cytokine,the expression of TNF-α and its receptor were observed.Results: After eight days of treatment, compared with cisplatin group, the levels of CEA,NSE,Pro GRP in serum,CD3+,CD4+,CD4+/ CD8+levels in serum peripheral blood and TNF-R1 expressions in tumor tissues of Yiqiyangyin formula group were increased( P < 0. 05),the contents of TNF-α and IL-6 and the expressions of TNF-α and TNF-R2 in tumor tissues of Yiqiyangyin formula group were significantly decreased( P <0. 05).Conclusion: Yiqiyangyin formula affects on advanced lung cancer cachexia, which may be related to the regulation of the immune and inflammatory cytokines expression.","PeriodicalId":15312,"journal":{"name":"Journal of Chinese medicinal materials","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36437599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Genetic Diversity and Cluster Analysis of Astragalus membranaceus in Gansu Province]. 甘肃省黄芪遗传多样性及聚类分析

Journal of Chinese medicinal materials Pub Date : 2016-06-01

Yi-qin Hou, Yan-ping Zhang, You-tai Shi, Xin-xing Liu, Yu-liang Chen

{"title":"[Genetic Diversity and Cluster Analysis of Astragalus membranaceus in Gansu Province].","authors":"Yi-qin Hou, Yan-ping Zhang, You-tai Shi, Xin-xing Liu, Yu-liang Chen","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the genetic status of Astragalus membranaceuse resources in Gansu province.Methods: Using SSR molecular marker technology for collection of Astragalus membranaceuse resources for genetic diversity analysis and clustering analysis.Results: Nine SSR primers were used on PCR amplification of 57 samples in six main areas of Gansu province,PCR products molecular weighted between 100 ~ 500 bp,the polymorphic loci was 82,the polymorphism rate was 97. 56%,and the average polymorphism information content was 0. 438. At the species level,the number of alleles was 1. 976,the effective number of alleles was 1. 459,Nei’ s genetic diversity was 0. 279,Shannon information index was 0. 431,the group of genetic diversity degree was 0. 248,the genetic differentiation among of population was 0. 117,the gene flow coefficient was 3. 775,and the genetic identity was 0. 896 ~ 0. 977.Conclusion: Astragalus membranaceuse resources of Gansu are relatively pure,and have abundant genetic diversity. The genetic variation mainly comes from the group of inside,the genes communication between populations is frequent,and the kinship between population is consistent with their geographic distance. In addition,the results of cluster analysis showed that the Core SSR primers can distinguish Astragalus and Hedysarum in the similarity of 0. 46,but Astragalus membranaceus var. mongholicus,Astragalus membranaceus and Astragalus tongolensis can’t be distinguished.","PeriodicalId":15312,"journal":{"name":"Journal of Chinese medicinal materials","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36437360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Research on the Correlation Between Soil Mineral Elements and NIR Fingerprint of Angelica sinensis]. [当归土壤矿质元素与近红外指纹图谱的相关性研究]。

Journal of Chinese medicinal materials Pub Date : 2016-06-01

Yao-peng Wang, Yan Fang, Zhi-rong Gu, Ya-li Wang

{"title":"[Research on the Correlation Between Soil Mineral Elements and NIR Fingerprint of Angelica sinensis].","authors":"Yao-peng Wang, Yan Fang, Zhi-rong Gu, Ya-li Wang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the correlation between soil mineral elements and NIR fingerprint of Angelica sinensis.Methods: The fingerprint of 130 batches of Angelica sinensis from 12 production regions were determined by near infrared spectra with integrating sphere and diffuse device of near infrared spectrometer. The determination of 15 kinds of mineral elements in the corresponding origin of soil mass fraction were determined by atomic absorption spectrometry or inductively coupled plasma mass spectrometry, grey correlation analysis and multivariate nonlinear regression method were used to analyze.Results: The soil mineral elements and their contribution were Cu > Pb, which were the main influencing factors of Angelica sinensis NIR fingerprint maximum absorbance on peak 7 249cm-1; the contribution of mineral elements in soil were Cr > Fe > Zn > Cd > Ca on peak 6 996 cm-1,there were positive correlation between Cr and Cd, and negative correlation between Fe and Ca; on peak 5 900 cm-1were Cu; on peak 5 000 cm-1were K > Ca > Zn; on peak 4 762 cm-1were K > Sb; on peak 4 651 cm-1were Ca > K > As > Cr, there were positive correlation between Ca and K, and the positive correlation or negative correlation between the square of As and Cr; the contribution of mineral elements in soil on peak 4 545cm-1were Ni > Cu > As; on peak 4 347 cm-1were Cd > Ca > As > Fe > K > Sb, and there were positive correlation among Cd, K, As and Fe.Conclusion: Angelica sinensis NIR fingerprint spectrum characteristics associate with a variety of soil mineral elements, which show the multiplicity and interactivity.","PeriodicalId":15312,"journal":{"name":"Journal of Chinese medicinal materials","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36437361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Chemical Constituents of Ethyl Acetate Extract from Viola biflora]. [堇菜乙酸乙酯提取物的化学成分]。

Journal of Chinese medicinal materials Pub Date : 2016-05-01

Wen-li Cong, Yu-ting Chen, Wen-bin Zhao, Zhang Zhang, Qi Wang

引用次数: 0

[Study on Deproteinization of Polysaccharides from Dioscorea nipponica]. [薯蓣多糖的脱蛋白研究]。

Journal of Chinese medicinal materials Pub Date : 2016-05-01

Xiao Song, Peng Zhao, Xi Duan, Chang-li Wang, Han-shuang Deng

引用次数: 0

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