[Study on Deproteinization of Polysaccharides from Dioscorea nipponica].

中药材 Pub Date : 2016-05-01

Xiao Song, Peng Zhao, Xi Duan, Chang-li Wang, Han-shuang Deng

引用次数: 0

Abstract

Objective: To screen the optimum method for deproteination of Dioscorea nipponica polysaccharides.

Methods: The ratio of protein removing and polysaccharides remaining were used as indicator,four deproteination methods were evaluated.

Results: PapainSevage method was the best one, in which the deproteination rate was 91. 24%,and 80. 12% of polysaccharide was remained. The optimum conditions for deproteinization were as follows, hydrolysing the substrates with 2. 0% papain( p H 7. 0) at 55 ℃ for 122 min, and one times with Sevage method.

Conclusion: The papain-Sevage is the best deproteination method for purifying Dioscorea nipponica polysaccharides.

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[薯蓣多糖的脱蛋白研究]。

目的:筛选薯蓣多糖的最佳脱蛋白方法。方法:以脱蛋白率和多糖残留量为指标，对4种脱蛋白方法进行评价。结果:PapainSevage法脱蛋白率为91，效果最好。24%, 80。剩余12%的多糖。脱蛋白的最佳条件为:用2。0%木瓜蛋白酶(h7)0)在55℃下放置122 min，用Sevage法放置1次。结论:木瓜蛋白酶法是纯化薯蓣多糖的最佳脱蛋白方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

中药材

自引率

0.00%

发文量

11364

期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.