{"title":"[Effect of Jiawei Yinchen Sini Decoction on the Production of Smad7 and CollagenⅠ,Ⅲ in Carbon Tetrachloride Induced Hepatic Fibrosis Model of Mice].","authors":"Jie Li, Jian-li Qiu, Hua Xu","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To explore the preventive effects of Jiawei Yinchen Sini Decoction on the gene expression of Smad7 and Collagen,Ⅲ in mice with hepatic fibrosis.</p><p><strong>Methods: </strong>48 male ICR mice were randomly divided into normal group, model group, colchicine group, JWYSN high dose group, medium dose group and low dose group, with 8 mice in each group. The hepatic fibrosis model was established by intraperitoneal injection of 30% CCl4( 1. 5 mg/kg, dissolved in olive oil). At the same time, the mice were treated with intragastric adminstration of drugs. After 14 days, the liver index was calculated; the levels of serum hyaluronic acid( HA) and laminin protein( LN) were detected by Enyme-linked immunosorbent assay; the pathological fibrosis change was observed by performing HE staining and was made a grading of hepatic fibrosis. mRNA expression of Smad7 and Collagen I, Ⅲ were detected by RT-q PCR analysis.</p><p><strong>Results: </strong>(1) Compared with model group, the liver index and levels of HA and LN in JWYSN medium dose group and low dose group were obviously decreased( P < 0. 05).( 2) The pathological results showed that, compared with model group, the hepatic fibrosis were significantly relief in different dose of JWYSN group( P < 0. 05).( 3) The expressions of Collagen Ⅰ mRNA in each treatment group were significantly lower than model group( P < 0. 05); In addition to JWYSN high dose group, the expressions of Smad7 mRNA in each treatment group increased significantly( P < 0. 05); In addition to JWYSN low dose group, the expression of CollagenⅢmRNA in each treatment group decreased significantly( P < 0. 05).</p><p><strong>Conclusion: </strong>Jiawei Yinchen Sini decoction can up-regulate the expressions of Smad7 mRNA and inhibit expressions of mRNA and CollagenⅢmRNA in mice with hepatic fibrosis,thus attenuate the development of hepatic fibrosis.</p>","PeriodicalId":15312,"journal":{"name":"中药材","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中药材","FirstCategoryId":"3","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To explore the preventive effects of Jiawei Yinchen Sini Decoction on the gene expression of Smad7 and Collagen,Ⅲ in mice with hepatic fibrosis.
Methods: 48 male ICR mice were randomly divided into normal group, model group, colchicine group, JWYSN high dose group, medium dose group and low dose group, with 8 mice in each group. The hepatic fibrosis model was established by intraperitoneal injection of 30% CCl4( 1. 5 mg/kg, dissolved in olive oil). At the same time, the mice were treated with intragastric adminstration of drugs. After 14 days, the liver index was calculated; the levels of serum hyaluronic acid( HA) and laminin protein( LN) were detected by Enyme-linked immunosorbent assay; the pathological fibrosis change was observed by performing HE staining and was made a grading of hepatic fibrosis. mRNA expression of Smad7 and Collagen I, Ⅲ were detected by RT-q PCR analysis.
Results: (1) Compared with model group, the liver index and levels of HA and LN in JWYSN medium dose group and low dose group were obviously decreased( P < 0. 05).( 2) The pathological results showed that, compared with model group, the hepatic fibrosis were significantly relief in different dose of JWYSN group( P < 0. 05).( 3) The expressions of Collagen Ⅰ mRNA in each treatment group were significantly lower than model group( P < 0. 05); In addition to JWYSN high dose group, the expressions of Smad7 mRNA in each treatment group increased significantly( P < 0. 05); In addition to JWYSN low dose group, the expression of CollagenⅢmRNA in each treatment group decreased significantly( P < 0. 05).
Conclusion: Jiawei Yinchen Sini decoction can up-regulate the expressions of Smad7 mRNA and inhibit expressions of mRNA and CollagenⅢmRNA in mice with hepatic fibrosis,thus attenuate the development of hepatic fibrosis.
期刊介绍：
"Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.
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