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Journal of Carbohydrate Chemistry最新文献

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Synthesis and nondestructive detailed structure characterization of carboxymethyl xylan from bagasse 甘蔗渣羧甲基木聚糖的合成及无损精细结构表征
IF 1 4区 化学
Journal of Carbohydrate Chemistry Pub Date : 2020-01-01 DOI: 10.1080/07328303.2020.1748643
Lei Wang , Xiangxiang Qin , Xueyan Miao , Haishan Chen , Yuheng Zhou , Aihua Cai
{"title":"Synthesis and nondestructive detailed structure characterization of carboxymethyl xylan from bagasse","authors":"Lei Wang ,&nbsp;Xiangxiang Qin ,&nbsp;Xueyan Miao ,&nbsp;Haishan Chen ,&nbsp;Yuheng Zhou ,&nbsp;Aihua Cai","doi":"10.1080/07328303.2020.1748643","DOIUrl":"10.1080/07328303.2020.1748643","url":null,"abstract":"<div><p>Carboxymethyl xylan (CMX) was synthesized and characterized. A method based on quantitative <sup>13</sup>C NMR was developed to obtain detailed structural information. The total degree of substitution (DS), partial DS at 2- and 3-<em>O</em>-positions, and the quantitation of each unit in CMX could be acquired simultaneously by this method without decomposing the polymer backbone, thus avoiding errors caused by polymer hydrolysis with traditional methods using HPLC and <sup>1</sup>H NMR spectrometry. Moreover, different from methods of direct detection of carboxyl groups, such as acidometric and potentiometric titrations, this method was not affected by probable impurity introduced during carboxymethylation.</p></div>","PeriodicalId":15311,"journal":{"name":"Journal of Carbohydrate Chemistry","volume":"39 4","pages":"Pages 131-144"},"PeriodicalIF":1.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328303.2020.1748643","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48465782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
GM3 trisaccharide biosynthesis and process optimization using engineered E. coli lysate and whole-cell catalysis 利用工程大肠杆菌裂解物和全细胞催化合成GM3三糖及其工艺优化
IF 1 4区 化学
Journal of Carbohydrate Chemistry Pub Date : 2020-01-01 DOI: 10.1080/07328303.2020.1788576
Lipeng Feng , Jie Shi , Haofei Hong , Zhifang Zhou , Zhimeng Wu
{"title":"GM3 trisaccharide biosynthesis and process optimization using engineered E. coli lysate and whole-cell catalysis","authors":"Lipeng Feng ,&nbsp;Jie Shi ,&nbsp;Haofei Hong ,&nbsp;Zhifang Zhou ,&nbsp;Zhimeng Wu","doi":"10.1080/07328303.2020.1788576","DOIUrl":"10.1080/07328303.2020.1788576","url":null,"abstract":"<div><p>An engineered <em>E. coli</em> strain BL21 (DE3, SiaB, PmST3, △<em>lacZ</em>, △<em>nanZ</em>) that simultaneously encoded CMP-Neu5Ac synthase (SiaB) and α-2-3-sialyltransferase (PmST3) was constructed to synthesize GM3 trisaccharide. The crude <em>E. coli</em> lysate could be readily used for biosynthesis of GM3 trisaccharide with a nearly quantitative yield under optimal conditions. In addition, this engineered <em>E. coli</em> strain as whole-cell catalyst could generate approximately 1 g of GM3 trisaccharide in 40 mL of medium under optimal conditions. The whole-cell catalyst could be recycled and reused for at least 3 rounds without obvious loss of enzymatic activity.</p></div>","PeriodicalId":15311,"journal":{"name":"Journal of Carbohydrate Chemistry","volume":"39 5","pages":"Pages 217-231"},"PeriodicalIF":1.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328303.2020.1788576","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42110190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structural analyses and engineering of the pmHAS enzyme to improve its functional performance: An in silico study pmHAS酶的结构分析和工程设计以提高其功能性能:一项硅片研究
IF 1 4区 化学
Journal of Carbohydrate Chemistry Pub Date : 2020-01-01 DOI: 10.1080/07328303.2020.1821041
Alireza Zakeri , Sepideh Khoshsorour , Mohsen Karami Fath , Navid Pourzardosht , Faezeh Fazeli , Saeed Khalili
{"title":"Structural analyses and engineering of the pmHAS enzyme to improve its functional performance: An in silico study","authors":"Alireza Zakeri ,&nbsp;Sepideh Khoshsorour ,&nbsp;Mohsen Karami Fath ,&nbsp;Navid Pourzardosht ,&nbsp;Faezeh Fazeli ,&nbsp;Saeed Khalili","doi":"10.1080/07328303.2020.1821041","DOIUrl":"10.1080/07328303.2020.1821041","url":null,"abstract":"<div><p>The <em>Pasteurella multocida</em> hyaluronic acid synthase (pmHAS) is reported to be able to solve the problem of hyaluronic acid (HA) polydispersity, while simplifying its purification process. In the present study, we tried to design a mutant pmHAS enzyme with improved functional properties. In this regard, several mutations were predicted and exerted within the active site of the enzyme. The obtained results showed that the mutant enzyme was more stable and was able to bind to its ligands with higher affinity. Given our results, the mutated enzyme could be used to produce HA more efficiently and prevent the breakdown of HA.</p></div>","PeriodicalId":15311,"journal":{"name":"Journal of Carbohydrate Chemistry","volume":"39 7","pages":"Pages 354-373"},"PeriodicalIF":1.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328303.2020.1821041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48312411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Synthesis of glycoimmunogen Tn-Thr-PS A1 via hydrazone bond and stability optimization of PS A1 monosaccharide mimics under vaccine development conditions 酰腙键合成糖免疫原Tn-Thr-PS A1及PS A1单糖模拟物在疫苗制备条件下的稳定性优化
IF 1 4区 化学
Journal of Carbohydrate Chemistry Pub Date : 2020-01-01 DOI: 10.1080/07328303.2019.1709975
F. Hossain , S. Nishat , S. Ghosh , S. Boga , G.T. Hymel , P.R. Andreana
{"title":"Synthesis of glycoimmunogen Tn-Thr-PS A1 via hydrazone bond and stability optimization of PS A1 monosaccharide mimics under vaccine development conditions","authors":"F. Hossain ,&nbsp;S. Nishat ,&nbsp;S. Ghosh ,&nbsp;S. Boga ,&nbsp;G.T. Hymel ,&nbsp;P.R. Andreana","doi":"10.1080/07328303.2019.1709975","DOIUrl":"10.1080/07328303.2019.1709975","url":null,"abstract":"<div><p>Previously, our group constructed several immunogens utilizing oxime linkage to conjugate a T-cell stimulatory zwitterionic polysaccharide PS A1 and tumor associated carbohydrate antigens (TACAs) in acetate buffer. Here, a semi-synthetic immunogen was synthesized using hydrazone conjugation between PS A1 and a glycopeptide hydrazide (α-D-GalNAc-L-Thr-NH-NH<sub>2</sub>) with an excellent loading in PBS buffer. To get robust immune response, the retention of zwitterionic character of PS A1 under vaccine construction conditions is essential. In this regard, the stability of embedded pyruvate acetal moiety in tetrasaccharide repeating unit of PS A1 can validate the retention of the dual charges. Therefore, rather than utilizing this highly immunogenic PS A1 fully, stability studies were performed with synthetic 1-thiophenyl-4,6-<em>O</em>-pyruvate acetal-D-galacto<em>pyranose</em> in varying acetate buffer pHs and time intervals. Furthermore, 1-propyl-D-galacto<em>furanose</em> was synthesized to mimick the D-Gal<em>f</em> of PS A1 to examine regioselective hydrazone and oxime formation with α-D-GalNAc-L-Thr-NH-NH<sub>2</sub> and α-D-GalNAc-ONH<sub>2</sub> moieties respectively.</p></div>","PeriodicalId":15311,"journal":{"name":"Journal of Carbohydrate Chemistry","volume":"39 2","pages":"Pages 107-129"},"PeriodicalIF":1.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328303.2019.1709975","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38987469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Study on the activity of Mori Fructus polysaccharides and its derivatives against acute alcoholic liver injury in mice 桑多糖及其衍生物抗小鼠急性酒精性肝损伤活性的研究
IF 1 4区 化学
Journal of Carbohydrate Chemistry Pub Date : 2020-01-01 DOI: 10.1080/07328303.2021.1895194
Xi Tan , Huaguo Chen , Xin Zhou
{"title":"Study on the activity of Mori Fructus polysaccharides and its derivatives against acute alcoholic liver injury in mice","authors":"Xi Tan ,&nbsp;Huaguo Chen ,&nbsp;Xin Zhou","doi":"10.1080/07328303.2021.1895194","DOIUrl":"10.1080/07328303.2021.1895194","url":null,"abstract":"<div><p>Four polysaccharides, MFPA1, MFPA2, MFPB1, and MFPB2, were isolated from Mori Fructus using DEAE-52 cellulose chromatography. MFPA1 (177 kDa) was composed of mannose, rhamnose, glucose, and xylose, and MFPB1 (165 kDa) was composed of mannose, rhamnose, galacturonic acid, glucose, and xylose, while MFPA2 (638 kDa) and MFPB2 (380 kDa) were consisted of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, xylose, and arabinose. These polysaccharides were sulfated to obtained Four sulfated polysaccharides, S-MFPA1, S-MFPA2, S-MFPB1, and S-MFPB2. The characteristic absorptive bands of purified polysaccharides and sulfated polysaccharides were determined by FT-IR. MFPA1, MFPB1, S-MFPA1, and S-MFPB1 showed excellent activities to activate alcohol dehydrogenase in vitro. Subsequently, it was found that MFPA1 had the strongest antiacute alcoholic liver injury activity through the experiments with acute alcoholic liver injury in mice. These results provide important scientific basis for <em>Mori fructus</em> polysaccharides as a potential therapeutic agent against acute alcoholic liver injury.</p></div>","PeriodicalId":15311,"journal":{"name":"Journal of Carbohydrate Chemistry","volume":"39 9","pages":"Pages 450-471"},"PeriodicalIF":1.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328303.2021.1895194","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44486431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Comparison on structure, chain conformation, and antioxidant activity of polysaccharides extracted from Liriopes radix with hot water and ultrasound 热水法和超声法提取枳壳多糖的结构、链构象及抗氧化活性比较
IF 1 4区 化学
Journal of Carbohydrate Chemistry Pub Date : 2020-01-01 DOI: 10.1080/07328303.2020.1746329
Xiaomei Wang , Shaobo Zhang , Zhixu Li , Miao Wang , Runguang Sun
{"title":"Comparison on structure, chain conformation, and antioxidant activity of polysaccharides extracted from Liriopes radix with hot water and ultrasound","authors":"Xiaomei Wang ,&nbsp;Shaobo Zhang ,&nbsp;Zhixu Li ,&nbsp;Miao Wang ,&nbsp;Runguang Sun","doi":"10.1080/07328303.2020.1746329","DOIUrl":"10.1080/07328303.2020.1746329","url":null,"abstract":"<div><p>The structures and chain conformations of two polysaccharides from <em>Liriopes radix</em>, WPRL-DS and UPRL-DS, isolated with hot water and ultrasound, respectively, were investigated by a combination of IR, GC, GC-MS, periodate oxidation, Smith degradation, NMR, AFM, and Congo red test. The results proved that the yield of ultrasonic extraction (UPRL-DS) was higher than that of hot water extraction (WPRL-DS). UPRL-DS had the same primary structure as WPRL-DS. However, UPRL-DS was spirally shaped whereas WPRL-DS was not. Both WPRL-DS and UPRL-DS were proved to have significant antioxidant activities as evidenced by their hydroxyl radical, superoxide anion, and 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activities. UPRL-DS exhibited more effective antioxidant activity than WPRL-DS in a concentration-dependent manner.</p></div>","PeriodicalId":15311,"journal":{"name":"Journal of Carbohydrate Chemistry","volume":"39 4","pages":"Pages 145-162"},"PeriodicalIF":1.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328303.2020.1746329","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49318954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
A systematic review on the significant roles of cyclodextrins in the construction of supramolecular systems and their potential usage in various fields 综述了环糊精在超分子体系构建中的重要作用及其在各个领域的潜在应用
IF 1 4区 化学
Journal of Carbohydrate Chemistry Pub Date : 2020-01-01 DOI: 10.1080/07328303.2020.1792919
R. Periasamy
{"title":"A systematic review on the significant roles of cyclodextrins in the construction of supramolecular systems and their potential usage in various fields","authors":"R. Periasamy","doi":"10.1080/07328303.2020.1792919","DOIUrl":"10.1080/07328303.2020.1792919","url":null,"abstract":"<div><p>Cyclodextrins (CDs) are oligosaccharides consisting of cyclic α(1,4)-linked glucopyranose subunits. They have cage-like three-dimensional structures. CDs have many applications due to their ability to form inclusion complexes. This review has addressed the historical background of CD discovery and highlighted the chemical and physical properties of cyclodextrins. In addition, the paper has also discussed the roles of CDs in supramolecular chemistry, the mechanisms involved in their formation of inclusion complexes, the techniques involved in characterizing inclusion complexes, and the applications of CDs in various fields.</p></div>","PeriodicalId":15311,"journal":{"name":"Journal of Carbohydrate Chemistry","volume":"39 5","pages":"Pages 189-216"},"PeriodicalIF":1.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328303.2020.1792919","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47866167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Chemical synthesis and preliminary biological evaluation of C-6-O-methyl-1-deoxynojirimycin as a potent α-glucosidase inhibitor α-葡萄糖苷酶抑制剂c -6- o -甲基-1-脱氧诺吉霉素的化学合成及初步生物学评价
IF 1 4区 化学
Journal of Carbohydrate Chemistry Pub Date : 2020-01-01 DOI: 10.1080/07328303.2019.1700995
Lin Wang , Tingting Liang , Zhijie Fang
{"title":"Chemical synthesis and preliminary biological evaluation of C-6-O-methyl-1-deoxynojirimycin as a potent α-glucosidase inhibitor","authors":"Lin Wang ,&nbsp;Tingting Liang ,&nbsp;Zhijie Fang","doi":"10.1080/07328303.2019.1700995","DOIUrl":"10.1080/07328303.2019.1700995","url":null,"abstract":"<div><p>A facile and efficient synthesis of 6-<em>O</em>-methyl-1-deoxynojirimycin <strong>4</strong> from commercially available methyl α-<em>D</em>-glucopyranoside in 10 steps and 25% overall yield was reported. The synthetic strategy was based on the regioselective protection/deprotection at 6-<em>O</em>-position and cyclization as the main reaction steps. Compound <strong>4</strong> was evaluated for its inhibitory effect (IC<sub>50</sub>) on α-glucosidase using an <em>in vitro</em> assay. It showed improved inhibitory activity compared to 1-deoxynojirimycin and acarbose.</p></div>","PeriodicalId":15311,"journal":{"name":"Journal of Carbohydrate Chemistry","volume":"39 1","pages":"Pages 36-49"},"PeriodicalIF":1.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328303.2019.1700995","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47540835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Glycosylation employing glycopyranosyl fluorides promoted by TiF4 under mild conditions 在温和条件下，TiF4促进甘氨酰氟的糖基化
IF 1 4区 化学
Journal of Carbohydrate Chemistry Pub Date : 2020-01-01 DOI: 10.1080/07328303.2020.1837151
Joachim Thiem , Matthias Wiesner
{"title":"Glycosylation employing glycopyranosyl fluorides promoted by TiF4 under mild conditions","authors":"Joachim Thiem ,&nbsp;Matthias Wiesner","doi":"10.1080/07328303.2020.1837151","DOIUrl":"10.1080/07328303.2020.1837151","url":null,"abstract":"<div><p>Glycopyranosyl fluorides are shown as efficient glycosyl donors by glycosylation of appropriate aglycon structures under mild conditions with Lewis acid catalysis in anhydrous ether or acetonitrile. Further direct reaction sequences gave naturally occurring disaccharide derivatives of biological interest.</p></div>","PeriodicalId":15311,"journal":{"name":"Journal of Carbohydrate Chemistry","volume":"39 8","pages":"Pages 398-414"},"PeriodicalIF":1.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328303.2020.1837151","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59686255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
2018 JCC Best Paper Award 2018年JCC最佳论文奖
IF 1 4区 化学
Journal of Carbohydrate Chemistry Pub Date : 2019-11-06 DOI: 10.1080/07328303.2019.1685796
Guo Zhongwu
{"title":"2018 JCC Best Paper Award","authors":"Guo Zhongwu","doi":"10.1080/07328303.2019.1685796","DOIUrl":"https://doi.org/10.1080/07328303.2019.1685796","url":null,"abstract":"I am delighted to announce that the winner of the 2018 JCC Best Paper Award is given to Miss Wei Chen at Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei, China, for her outstanding research paper “Mechanism investigations of the activation process of S-2-[(propan-2-yl)sulfinyl]benzyl (SPSB) glycosides” published in the Journal of Carbohydrate Chemistry, 2018, 37, 498. The JCC Best Paper Award is established by the Journal to recognize the best paper published in the journal each year. Miss Chen was born in 1992 and received her B.S. degree in Pharmaceutical Science in 2014 from Huazhong University of Science and Technology, Wuhan, Hubei, China. She is currently pursuing her PhD degree under the supervision of Professor Qian Wan at Huazhong University of Science and Technology. In her graduate study, Miss Chen has been focused on the mechanism investigations of glycosylation.","PeriodicalId":15311,"journal":{"name":"Journal of Carbohydrate Chemistry","volume":"38 1","pages":"529 - 529"},"PeriodicalIF":1.0,"publicationDate":"2019-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328303.2019.1685796","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41564144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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