Journal of Biomaterials and Tissue Engineering最新文献

{"title":"Long Non-Coding RNA-X-Inactive Specific Transcript Promotes the Retinal Ganglion Cell Survival After Optic Nerve Crush Injury by Upregulating miR-36","authors":"Qingjia Ren, Junjun Zhang","doi":"10.1166/jbt.2023.3265","DOIUrl":"https://doi.org/10.1166/jbt.2023.3265","url":null,"abstract":"Retinal ganglion cells (RGC) axons participate in the construction of optic nerve, and prevent the damage of RGC during acute optic nerve injury. IncRNA-XIST is crucial for RGC apoptosis. Our study intends to assess IncRNA-XIST’s role in the regulation of RGC apoptosis in an attempt\u0000 to provide a theoretical basis for treating optic nerve crush injury. Two genotypes of mice (wild-type and miR-36 KO) were used to establish an optic nerve crush injury model to investigate the regulatory role of IncRNA-XIST gene in RGCs apoptosis. These mice were then randomly assigned into\u0000 control group (WT), injury group, and XIST/injury group. The changes of apoptotic genes and proteins in retinal ganglion cells were analyzed by qPCR, WB and TUNEL staining. In wild-type mice, RGC apoptosis was significantly increased after optic nerve compression injury, and the expression\u0000 of Bax and Bad was significantly increased. When the LncRNA-XIST gene was overexpressed before retinal crush injury, the apoptosis of retinal ganglion cells was significantly reduced, and Bax and Bad levels were decreased as compared with model group of optic nerve injury. The results showed\u0000 that in wild-type mice, overexpression of IncRNA-XIST gene promoted the survival of RGC after optic nerve crush injury. In addition, upregulation of IncRNA-xist expression in miR-36 KO mice did not reduce retinal ganglion cell apoptosis and alter the apoptotic protein expression after optic\u0000 nerve crush injury. Defects of miR-36 alone or overexpression of XIST gene do not cause morphological damage of retina in mice. In mouse ganglion cells, miR-36 expression was up-regulated in both injured cells and overexpressed XIST gene. However, up-regulation of miR-36 caused by overexpression\u0000 of XIST gene was more obvious. In addition, in vivo studies of wild-type mice, it was found that overexpression of XIST reduced retinal ganglion cell apoptosis, and this effect was abolished in miR-36 KO mice. In conclusion, lncRNA-XIST reduces ganglion cell apoptosis by upregulating\u0000 miR-36 and promotes the survival of RGC after nerve crush injury.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47489385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone Marrow Mesenchymal Stem Cells (BMSC) Exosome-Derived mR-512-5p Inhibits the Proliferation and Apoptosis of Glioblastoma Cells","authors":"Feng Qiu, Leyi Xu, Li Gong, Lingjun Kong, Jue Zhang, Z. Fei","doi":"10.1166/jbt.2023.3266","DOIUrl":"https://doi.org/10.1166/jbt.2023.3266","url":null,"abstract":"This study discusses the mechanism of miR-512-5p derived from BMSC in restraining the proliferation and prompting apoptosis of GBM. BMSC exosome was obtained through ultra-centrifugation and assessed by TEM. The positive presentation of CD63 and HSP70 was detected with Western Blot.\u0000 The GBM cell line LN229 was divided into WM set, NC set, and ZR set followed by analysis of cell proliferation by MTT method, invasive ability by Tranwell chamber, apoptotic rate by FCM and the expression of JAG1 and notch2 by Western Blot. miR-512-5p level in LN229 cells was significantly\u0000 lower than U87MG and SHG44 cells. There was positive expression of CD63 and HSP70 in exosome. LN229 cell proliferation was restrained by the drug. ZR set had lower cell proliferation rate and invasive quantity and higher apoptotic rate than WM set and NC set. The protein expressions of JAG1\u0000 and notch2 in ZR set was reduced compared with WM set and NC set (P <0.05) without difference between NC set and WM set (P >0.05). In conclusion, GBM cell proliferation could be restrained and apoptosis could be increased by miR-512-5p derived from BMSC through targeting\u0000 JAG1. It could provide a brand-new therapeutic strategy for the treatment on GBM.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"122 3","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41284489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Downregulated miR-100 Result in High Expression Levels of Chemokine (CXC Motif) Receptor 7 That Predict Recurrence of Lung Cancer","authors":"Liu-Jie Gao, Ting-Ting Shao, Wan-Zhen Zheng, Jiguo Ding, Jun Li","doi":"10.1166/jbt.2023.2619","DOIUrl":"https://doi.org/10.1166/jbt.2023.2619","url":null,"abstract":"MicroRNAs (miRs) have been proved to play significant role in both the tumorigenesis and recurrence of lung cancer (LA). However, the identification of which miR participated in the recurrence of LA, as well as the underlying mechanism are poorly understood. Here in our article, quantitative\u0000 analysis of the miR-100 expression in non-recurrent and recurrent LA tissues using real-time PCR (RT-qPCR) revealed that, compared to non-recurrent LA, the expression of miR-100 was significantly downregulated in recurrent ones. Especially, the chemokine (CXC motif) receptor 7 (CXCR7) level\u0000 was negatively correlated with the expression of miR-100 in both non-recurrent and recurrent LA cell lines derived from corresponding LA tissues. The following in vitro experiments also confirmed that either miR-100 overexpression or CXCR7 depletion decreased proliferation of recurrent\u0000 LA cells. In conclusion, miR-100 may act as a recurrence suppressor in LA through the regulation of CXCR7 expression, which might be a promising target for the prevention of LA recurrence.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42200246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety Evaluation of Rock Hyrax (Procavia capensis) Hyraceum: Oral Toxicity Study in Rats","authors":"Manei M. Aljedaie, Mohamed A. Akela","doi":"10.1166/jbt.2023.3270","DOIUrl":"https://doi.org/10.1166/jbt.2023.3270","url":null,"abstract":"Urine from domestic animals has traditionally been used to treat multiple human diseases. Rock hyrax hyraceum (RHH) is a dark brown resin-like substance made up of accumulated feces and urine of rock hyraxes, used in traditional African medicine to treat snake and scorpion bites, as\u0000 well as colic, hysteria, epilepsy, and Parkinson’s disease. As there is no scientific backing for the utilization of hyrax hyraceum, there is an urgent need to establish its safety as a medicine. Furthermore, toxicity evaluation facilitates the identification of factors for clinical\u0000 surveillance of the potential side effects, as well as the estimation of a preliminary hygienic dose level and dosing frequency range for human clinical trials. The safety of RHH was assessed by administering 2.5 and 5 gm/kg doses of hyraceum crystals dissolved in de-ionized water and administered\u0000 to rats orally. We evaluated the acute toxicity profile, as well as the effect of RHH on liver and kidney function and histopathological investigations. The results of this investigation showed that consuming RHH does not have any hazardous effects on liver and kidney function and histopathological\u0000 investigations. Drinking RHH did not cause toxicity. These findings are consistent with those of previous studies of drinking cows and camel urine. We encourage more research on hyraceum composition in relation to rock hyrax habitat areas and eating patterns, as we believe it will help us\u0000 understand why people practice drinking hyraceum as a medicine.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48407663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long Non-Coding RNA LINC02532 Mediates p-AKT to Regulate Gastric Cancer Cell Activities Through Targeting miR-362-5p","authors":"Kaiyu Li, Chunbo Li, Jin-Chao Zhao, Xin Ge, Nan Wang, Yu Sun","doi":"10.1166/jbt.2023.3271","DOIUrl":"https://doi.org/10.1166/jbt.2023.3271","url":null,"abstract":"LINC02532 and miR-362-5p modulates gastric cancer (GC) cell activities. Herein, we elucidated the role of LINC02532 targeting miR-362-5p to mediate p-AKT in GC cells, aiming to provide a theoretical basis for clinical treatment. Human GC cells were treated with si-LINC02532, si-NC,\u0000 LINC02532+miR-362-5p inhibitor and p-AKT inhibitor. LINC02532 and miR-362-5p expression was determined by RT-qPCR and p-AKT expression was detected. Transwell assay assessed cell invasion and migration upon treatment and the targeting relationship of LINC02532 and miR-362-5p was evaluated.\u0000 A positive expression of LINC02532 and miR-362-5p was detected in each group of GC cells. The expression of LINC02532 was up-regulated (2.95 ± 0.23) and miR-362-5p was down-regulated (0.35 ± 0.08). Silence of LINC02532 significantly suppressed GC cell behaviors and inhibited\u0000 migration speed of cancer cells, while p-AKT inhibitor treatment resulted in a decrease in the number of invaded and migrated cells. Combination of LINC02532 and miR-362-5p inhibitor was not effective as previous two treatments, but still decreased cell migration and invasion (p <\u0000 0.05). The luciferase experiment indicated LINC02532 targeted miR-362-5p. Down-regulation of LINC02532 also reduced p-AKT protein expression. p-AKT inhibitor group had a lower level of p-AKT protein, followed by LINC02532+miR-362-5p inhibitor group, and si-NC group. In conclusion, silence\u0000 of LINC02532 reduces miR-362-5p and p-AKT protein expression in GC cells to suppress GC cell growth through inhibition of p-AKT signaling pathway.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46968075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Epithelial FTL Expression as an Important Predictor for Prognosis of Colon Adenocarcinoma","authors":"Ziyang Sun, Siqi Zhang, Lei Zhao, Shuhao Wang, Zhen-dong Lin, A. Jin","doi":"10.1166/jbt.2023.3275","DOIUrl":"https://doi.org/10.1166/jbt.2023.3275","url":null,"abstract":"Ferroptosis has been shown to be a non-self apoptotic, iron ion-catalysed, controlled cell death process involving cellular lipid oxides. The gnes that may affect ferroptosis may be candidate biomarkers. But the value and predictive value of ferroptosis-related genes in colorectal adenocarcinoma\u0000 (COAD) are not fully understood. Therefore, we analyzed the expression status in COAD and healthy colon tissue using GEO, GEPIA and HPA databases. The role of predictive and genetic alterations of ferroptosis-related genes were investigated using cBioPortal, HPA, PrognoScan, STRING, GeneMANIA\u0000 and LinkedOmics databases. Then, immunohistochemistry staining was performed to detect the both epithelial and stromal expression of FTL in COAD. Using FerrDb and GEO databases, the transcript levels of XBP1, TSC22D3 and YWHAE were identified to correlate with advanced\u0000 tumor stage, FTL, DUSP1, TSC22D3 and GDF15 were related to poor prognosis of COAD. As immunohistochemistry staining shown that FTL was significantly up-regulated in COAD tissues. Cox survival analysis and KM survival analysis indicated that high epithelial FTL levels\u0000 is associated with a poor prognosis in COAD.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43891859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Semaphorin 4C Plays a Key Role in Colorectal Cancer Cells Development","authors":"Hongyue Lin, Yuzhu Wu, Jinping Chen, Shurong Huang, Yang Zeng, Wei Zheng","doi":"10.1166/jbt.2023.3264","DOIUrl":"https://doi.org/10.1166/jbt.2023.3264","url":null,"abstract":"Objective: Purpose of this work was to discuss effects and mechanisms of Sema 4C in colon cancer development. Results: Sema4C were significantly upregulated in cancer tissues (P <0.001). Following transfection, the expression levels of Sema4C mRNA were significantly\u0000 downregulated in the si-Sema4C groups compared with those in the si-negative control groups of both HT-29 and SW620 cells (both P <0.001). The apoptotic rate was significantly increased, while the invasive and wound healing rates were significantly suppressed in the si-Sema4C groups\u0000 of HT-29 and SW620 cells (both P < 0.001). The results of the RT-qPCR and western blotting analyses revealed that PI3K, AKT, MMP-2 and MMP-9 mRNA and protein expression levels, respectively, were significantly downregulated, while caspase-3 mRNA and protein expression levels were\u0000 significantly upregulated in the si-Sema4C groups of HT-29 and SW620 cells. Conclusion: The findings of the present study demonstrated that the knockdown of Sema4C expression suppressed CRC cell biological activities by regulating the PI3K/AKT signaling pathway. Therefore, Sema4C may\u0000 act as an oncogene in CRC.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43014107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sealing Efficiency of BeeFill 2in1 Filling Technique and Cold Lateral Compaction Technique","authors":"Ning Zhang, Jiuyu Ge","doi":"10.1166/jbt.2023.3231","DOIUrl":"https://doi.org/10.1166/jbt.2023.3231","url":null,"abstract":"Objective: To discuss sealing properties between BeeFill 2in1 warm vertical compaction technique and cold lateral compaction technique. Methods: Group1 BeeFill 2in1, Group 2: cold lateral compaction. The leakage specimens were measured using the transverse root sectioning,\u0000 after 1 month. Results: The statistical analysis showed that the frequency of section with void, the percentage of void area and the percentage of sections at 1 mm–13 mm levels, the BeeFill 2in1 group were lower than those of the cold lateral compaction group. Conclusion:\u0000 BeeFill 2in1 warm vertical compaction technique has its advantage in dealing with the long oval canal.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47933752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Icariside II-Loaded Calcium Phosphate Cement Scaffolds Combined with Bone Marrow Mesenchymal Stem Cells for Osteogenesis","authors":"Guangming Luo, Zhangshun Yao, Yan Yang, Jiachuan Chai, Lilin Fu","doi":"10.1166/jbt.2023.3268","DOIUrl":"https://doi.org/10.1166/jbt.2023.3268","url":null,"abstract":"Purpose: Previous studies have demonstrated the osteogenic effects of icariside II (ICSII), which is a metabolic product of the prenylated active flavonol icariin (ICA) from the roots of Epimedium. However, the in vivo osteogenic effects of ICSII remain unclear. Thus,\u0000 in this study, we evaluated the osteogenic effects of ICSII in vivo. Methods: Complexes of calcium phosphate cement (CPC) and bone marrow mesenchymal stem cells (BMSCs) with or without ICSII were subcutaneously implanted into nude mice (ectopic osteogenesis test) and into tooth\u0000 sockets in beagles after maxillary canine tooth extraction (in situ osteogenesis test). The samples were harvested at different time points, and the in vivo osteogenic effect of the ICSII on the BMSCs was evaluated by histology, microcomputed tomography (micro-CT) and the bone\u0000 mineralization apposition rate (MAR). Results: The proliferation and viability of BMSCs in the ICSII-loaded CPC scaffold were significantly increased (P < 0.01). The new bone area and MAR in the CPC+BMSC+ICSII group were greater than those in the CPC+BMSC group (P <\u0000 0.05), but there was no significant difference in markers evaluated by immunohistochemistry and integrated optical density (IOD) analysis (P > 0.05), with the exception of Runx-2 expression in the CPC+BMSC+ICSII group. After 2 months, the bone mineral content (BMC) and specific bone\u0000 surface (bone surface divided by bone volume, BS/BV) were significantly increased (P < 0.05) in the CPC+BMSC+ICSII group compared with the CPC+BMSC group. The bone mineral density (BMD), bone volume divided by total volume (BV/TV), and trabecular number (Tb.N) were increased in the\u0000 CPC+BMSC+ICSII group, but the differences were not significant (P > 0.05). Conclusions: Our results suggest that ICSII can likely induce bone formation by BMSCs and be used as a promising factor for building scaffold composites in bone tissue engineering.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45735147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA-608 Regulates Epithelial-To-Mesenchymal Transition to Inhibit Malignant Behaviors of Glioma Cells Through Nuclear Factor I-C Signaling Pathway","authors":"Yuning Ma, Jing Cao, Tao Fan","doi":"10.1166/jbt.2023.3273","DOIUrl":"https://doi.org/10.1166/jbt.2023.3273","url":null,"abstract":"MiR-608 is expressed in bladder cancer, colon cancer, prostate cancer risk, and exerts inhibitory role in gastric cancer and invasion of three negative malignant breast cancer by inhibiting the NIFC, but its role in the biology of glioma is unclear, so the purpose of this study was\u0000 to explore MiR-608’s role in glioma, and further explore whether NFIC signal pathways are involved in the role of MiR-608 in glioma. We obtain glioma tissues and normal tissues to detect the expression of miR-608 by PCR. miR-608 mimics transfection or joint with NFIC expression plasmid\u0000 were transfected into A172, and LN229 glioma cells followed by analysis of cell proliferation, clone formation, invasion and migration, and cell apoptosis. Compared to normal tissue, miR-608 expression in glioma tissues was decreased. After transfection of miR-608 mimics, miR-608 level, cell\u0000 proliferation activity, invasion and migration activity increased significantly, and apoptosis reduced, in addition, the dual luciferase report gene and protein imprinting analysis showed NFIC to be a target of miR-608. NFIC transfection reversed miR-608’s role in glioma cells. In conclusion,\u0000 microRNA-608 inhibits malignant invasion and migration of glioma cells via NFIC signaling.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41576697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}