用全合成DNA基因组重建溶瘤性马拉巴MG1病毒

IF 0.1 4区 医学
A. Mahmoud, Omar A. Albaradie, Abdulaziz M. Moglan, Fayhan J. Alroqi, A. Alkayyal
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引用次数: 0

摘要

溶瘤病毒(OVs)被设计成在癌细胞内选择性复制并摧毁它们,同时也诱导针对病毒和肿瘤的免疫反应。Maraba MG1毒株是Maraba病毒的双突变株,它优先靶向并杀死癌细胞,同时通过干扰素依赖机制将对正常细胞的伤害降到最低。在临床前肿瘤模型中,MG1显示出强大的抗肿瘤作用。在这里,我们证明了使用合成DNA基因组技术来设计MG1的可行性,通过修改其常用的限制性内切酶位点来开发生物类似的溶瘤病毒,从而实现简单的一步克隆过程。精确修改病毒基因组序列的能力可以更好地控制其特性,并且简化的基因插入过程加速了新疗法的开发。我们的平台将支持这种病毒作为癌症治疗的翻译,并为个性化MG1免疫治疗提供一个简化的平台。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Reconstruction of the Oncolytic Maraba MG1 Virus from a Fully Synthetic DNA Genome
Oncolytic viruses (OVs) are engineered to replicate selectively within cancer cells and destroy them while also inducing an immune response against the virus and the tumor. The Maraba MG1 strain is a double mutant of the Maraba virus that preferentially targets and kills cancer cells while minimizing harm to normal cells through interferon-dependent mechanisms. In preclinical tumor models, MG1 has demonstrated potent antitumor effects. Here, we demonstrate the feasibility of using synthetic DNA genome technology to engineer MG1 to develop a biosimilar oncolytic virus by modifying one of its commonly used restriction enzyme sites for an easy one-step cloning process. The ability to precisely modify the genome sequence of the virus allows greater control over its properties, and the simplified process of gene insertion accelerates the development of new therapies. Our platform will support the translation of this virus as a cancer treatment and provide a streamlined platform for personalized MG1 immunotherapy.
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