Journal of biomedical nanotechnology最新文献_第8页

Antibacterial Effect of Nanostructured Cuprous Gold and Silver Oxide Composite Nanoshell on Keratitis 纳米结构亚铜金和氧化银复合纳米壳对角膜炎的抗菌效果

IF 2.9 4区医学

Journal of biomedical nanotechnology Pub Date : 2024-02-01 DOI: 10.1166/jbn.2024.3764

Chen Wang, Yang Liu, Mingchang Zhang

{"title":"Antibacterial Effect of Nanostructured Cuprous Gold and Silver Oxide Composite Nanoshell on Keratitis","authors":"Chen Wang, Yang Liu, Mingchang Zhang","doi":"10.1166/jbn.2024.3764","DOIUrl":"https://doi.org/10.1166/jbn.2024.3764","url":null,"abstract":"We developed novel inorganic nanomaterials to combat drug-resistant bacterial infections in keratitis. These infections cause rapid severe corneal ulcers. Traditional antibiotics face challenges due to bacterial resistance. We investigated new therapies by designing nanomaterials. In\u0000 an animal model of diabetic keratitis, we studied the materials’ antibacterial properties and mechanisms. In vitro, nanomaterials displayed strong antibacterial effects, confirmed by quantitative analysis. In vivo, using thermal imaging, wound closure monitoring, clinical\u0000 scores, and histopathology, we demonstrated nanomaterials’ efficacy against infections. Toxicity evaluations, including weight monitoring, hemolysis, biochemical, hematological analyses, and organ histology, revealed no adverse effects on the body or organs. Confocal microscopy showed\u0000 effective bacterial eradication using nanomaterials combined with near-infrared laser treatment. Minimal impact on red blood cells was observed at therapeutic concentrations. Nanomaterials, particularly gold-silver-cuprous oxide composite nanoshells, demonstrated potent resistance against\u0000 drug-resistant infections. Photothermal treatment using nanomaterials and near-infrared laser showed promise without harming normal tissues, blood, or organs. Our findings offer a potential clinical solution for keratitis treatment.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139814251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current Advances in Single-Cell RNA Sequencing in Diabetic Retinopathy 糖尿病视网膜病变中单细胞 RNA 测序的最新进展

IF 2.9 4区医学

Journal of biomedical nanotechnology Pub Date : 2024-02-01 DOI: 10.1166/jbn.2024.3770

Kangqi Zhao, Ming Hao, Qian Xu, Hong-Xue Li, Cheng-Ye Xu, Ziyu Meng, H. Kuang

{"title":"Current Advances in Single-Cell RNA Sequencing in Diabetic Retinopathy","authors":"Kangqi Zhao, Ming Hao, Qian Xu, Hong-Xue Li, Cheng-Ye Xu, Ziyu Meng, H. Kuang","doi":"10.1166/jbn.2024.3770","DOIUrl":"https://doi.org/10.1166/jbn.2024.3770","url":null,"abstract":"With the development of high-throughput sequencing technology, humans have been able to conduct large-scale analysis of DNA sequence, chromatin structure, RNA transcripts, proteins, metabolites and other genomes and their products. Traditional high-throughput transcriptome sequencing\u0000 techniques based on tissue samples (RNA Seq) are used to centrally sequence thousands of cells, each of which varies in size, protein levels, and mRNA expression transcription. Measuring the average of multiple cells grouped together can mask significant differences in gene expression between\u0000 cells. Single-cell RNA sequencing is a technique for high-throughput sequencing of the genome, transcriptome, and epigenome at the single-cell level. Based on the single cell RNA transcription map, the intraocular cells can be distinguished from other subtypes, and the different subtypes are\u0000 found to have significant differences in morphology, physiology and specific expression genes. In recent years, the application of single-cell RNA sequencing technology in the field of ophthalmology has increased, mainly including cell type and cell subtype identification, retinal development\u0000 process, and eye disease research. This paper systematically summarized the latest application of single-cell sequencing technology in the field of diabetic retinopathy, and summarized marker genes and potential therapeutic targets. It has guiding significance for the clinical treatment of\u0000 diabetic retinopathy.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139820509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Iron Oxide Nanoparticles Carrying microRNA-124 Promote Ferroptosis in Treatment of Prostate Cancer 携带 microRNA-124 的氧化铁纳米粒子在治疗前列腺癌中促进铁凋亡

IF 2.9 4区医学

Journal of biomedical nanotechnology Pub Date : 2024-02-01 DOI: 10.1166/jbn.2024.3782

Min Liu, Chuanbing Xu, Huichao Dong, Dongsheng Jia, Dongfang Hao, Ruozen Rong, Yao Peng

{"title":"Iron Oxide Nanoparticles Carrying microRNA-124 Promote Ferroptosis in Treatment of Prostate Cancer","authors":"Min Liu, Chuanbing Xu, Huichao Dong, Dongsheng Jia, Dongfang Hao, Ruozen Rong, Yao Peng","doi":"10.1166/jbn.2024.3782","DOIUrl":"https://doi.org/10.1166/jbn.2024.3782","url":null,"abstract":"Prostate cancer (PCa) is a common malignancy among men worldwide. Iron oxide (Fe3O4) nanoparticles (NPs) exhibit great potential in gene delivery and studies have noted the inhibitory effect of miR-124 on PCa cell growth. Herein, we identified the therapeutic effect\u0000 of Fe3O4 NPs carrying miR-124 in PCa and clarified its mechanism of action in inhibiting progression of PCa. After preparation of Fe3O4 NPs carrying miR-124, human PCa cell line PC3 was treated with miR-124-Fe3O4 NPs when ferroptosis\u0000 inducer FIN56, ferroptosis inhibitor Liproxstatin-1, Phosphatase and tensin homolog (PTEN) inhibitor SF1670 or PTEN activator Oroxin B were added for transfection. Afterwards, assays were conducted to evaluate PCa cell biological activities. Additionally, we determined expression of PTEN and\u0000 AKT and ferroptosis-related protein GPX4 and SLC7A11 in each group. We confirmed anticancer effects of miR-124-Fe3O4 NPs in PCa, as they suppressed PC3 cell proliferation and migration, and induced apoptosis. Compared with miR-124-Fe3O4 + Liproxstatin-1\u0000 group, the expressions of GPX4 and SLC7A11 proteins in miR-124-Fe3O4 group were elevated. The advent of PTEN activator Oroxin B decreased proliferative ability of PCa cells, and SF1670 treatment decreased PTEN level and elevated AKT level. With highest apoptotic rate\u0000 of PCa cells in miR-124-Fe3O4 + Oroxin B + FIN56 group, intervention of SF1670 or Liproxstatin-1 changed the cell viability, while Oroxin B caused decreased AKT level and increased level of ferroptosis-related proteins. miR-124-Fe3O4 NPs hinder PCa\u0000 progression by promoting ferroptosis and cell apoptosis through regulation of PTEN/Akt pathway.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139878141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the Mechanism and Protection of Salt-Sensitive Hypertensive Rats’ Myocardial Fibrosis by Regulating Striatin with Sacubatrovalsartan 用沙考巴曲沙坦调节纹蛋白对盐敏感性高血压大鼠心肌纤维化的机制和保护作用研究

IF 2.9 4区医学

Journal of biomedical nanotechnology Pub Date : 2024-02-01 DOI: 10.1166/jbn.2024.3766

Qingxian Tu, Qianhang Xia, Meihong Chen, Haiyan Zhou, Qianfeng Jiang, Wei Li

{"title":"Study on the Mechanism and Protection of Salt-Sensitive Hypertensive Rats’ Myocardial Fibrosis by Regulating Striatin with Sacubatrovalsartan","authors":"Qingxian Tu, Qianhang Xia, Meihong Chen, Haiyan Zhou, Qianfeng Jiang, Wei Li","doi":"10.1166/jbn.2024.3766","DOIUrl":"https://doi.org/10.1166/jbn.2024.3766","url":null,"abstract":"This study aims to explore the relationship between STRN, TGF-β1, Caspase-3, PD-1 expression, and myocardial fibrosis in salt-sensitive hypertensive rats. It investigates the correlation between STRN expression and myocardial fibrosis, along with the protective effects of\u0000 Sacubitril/Valsartan (ARNI). Fifteen 18-week-old rats were divided into three groups: Control, high salt (SSH), and ARNI+SSH. Blood pressure was monitored weekly for 8 weeks. Echocardiography evaluated cardiac parameters, while H&E and Masson staining visualized myocardial morphology and\u0000 fibrosis. Immunohistochemistry measured protein expression of collagen-1, collagen-3, TGF-β1, PD-1, Caspase-3, and STRN. Western blot assessed STRN protein levels. High-salt diet increased fibrosis, collagen expression, TGF-β1, PD-1, Caspase-3, and reduced STRN expression\u0000 compared to Control (P < 0.05). ARNI treatment decreased fibrosis, collagen expression, TGF-β1, PD-1, Caspase-3 (P <0.05), and increased STRN expression compared to SSH (P <0.05). STRN expression correlated positively with myocardial fibrosis. ARNI\u0000 demonstrated potential in attenuating fibrosis by modulating STRN expression and suppressing apoptosis and inflammation in salt-sensitive hypertensive rats.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139881822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Simvastatin-Loaded Nanoliposome Delivery System for Sepsis-Induced Acute Lung Injury 治疗脓毒症诱发的急性肺损伤的辛伐他汀载体纳米脂质体输送系统

IF 2.9 4区医学

Journal of biomedical nanotechnology Pub Date : 2024-02-01 DOI: 10.1166/jbn.2024.3805

Jianhai Yang, Yue Yue

{"title":"A Simvastatin-Loaded Nanoliposome Delivery System for Sepsis-Induced Acute Lung Injury","authors":"Jianhai Yang, Yue Yue","doi":"10.1166/jbn.2024.3805","DOIUrl":"https://doi.org/10.1166/jbn.2024.3805","url":null,"abstract":"To enhance the treatment of acute lung injury (ALI) induced by sepsis and optimize the clinical efficacy of simvastatin (SV), we develop SV-loaded nanoliposomes (SV/NLC) as a novel drug delivery system. The NLCs exhibited a particle size of approximately 165 nm, which increased to around\u0000 195 nm upon SV loading. NLCs significantly prolonged the half-life of SV by nearly five-fold and improved its penetration into EA.hy926 cells, demonstrating excellent biocompatibility and targeted delivery for ALI therapy. In the rat model of ALI, the SV/NLC effectively reduced the lung wet/dry\u0000 ratio and the levels of inflammatory factor and albumin in the alveoli, thus improving the alveolar gas exchange function and blood oxygenation. The SV/NLC group demonstrated superior suppression of oxidative stress within lung tissues compared to other groups. Notably, treatment with SV reduction\u0000 in TLR4, MyD88, and NF-κB P65 levels in lung tissues from ALI rat models. This effect was particularly pronounced in the SV/NLC group. Furthermore, SV can effectively mitigate inflammatory responses and oxidative stress in ALI treatment by modulating the TLR4/NF-κB\u0000 signaling pathway. In conclusion, our findings suggest that SV can exert therapeutic effects against sepsis-induced ALI through inhibition of the TLR4/NF-κ and mitigate inflammatory response and oxidative stress.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139822202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Upregulation of LncRNA-LHX2 Promotes Hepatocellular Carcinoma Proliferation, Migration, Invasion and Metastasis via Targeting VEGFA by Sequestering of MiR-939-5p 通过封存 MiR-939-5p 靶向 VEGFA，上调 LncRNA-LHX2 促进肝细胞癌增殖、迁移、侵袭和转移

IF 2.9 4区医学

Journal of biomedical nanotechnology Pub Date : 2024-02-01 DOI: 10.1166/jbn.2024.3776

Caiyue Fang, Ruibo Lin, Suqin Gan, Hong Wang, Chenghui Huang

{"title":"Upregulation of LncRNA-LHX2 Promotes Hepatocellular Carcinoma Proliferation, Migration, Invasion and Metastasis via Targeting VEGFA by Sequestering of MiR-939-5p","authors":"Caiyue Fang, Ruibo Lin, Suqin Gan, Hong Wang, Chenghui Huang","doi":"10.1166/jbn.2024.3776","DOIUrl":"https://doi.org/10.1166/jbn.2024.3776","url":null,"abstract":"Due to the challenges in early diagnosis and lack of specific biomarkers, liver cancer remains one of the most prevalent and lethal tumor types. Numerous studies have shown that long noncoding RNA (lncRNA) plays a crucial role in the regulation of various malignant tumors, including\u0000 liver cancer. Here, we discussed the function and effect of LncRNA-LHX2 in the tumorigenesis and progression of liver cancer, which was significantly upregulated in liver cancer tissues, compared to the benign liver tissues. To improve the accuracy and efficiency of tests like qRT-PCR, we\u0000 employed nano-magnetic beads for nucleic acid extraction from tissues and cells. In our experiments using HepG2 cells, silencing of LncRNA-LHX2 effectively suppressed cell proliferation, migration, and invasion by interacting with miR-939-5p, which targets VEGFA. Interestingly, overexpression\u0000 of miR-939-5p also impaired malignant functions of HepG2 cells. However, simultaneously inhibition of miR-939-5p expression can partially restored the inhibitory effect on HepG2 cells resulting from LncRNA-LHX2 knockdown. Consistently, our in vivo results from tumor mice model also\u0000 suggested that knockout of LncRNA-LHX2 inhibited the tumor growth and suppressed epithelial mesenchymal transition (EMT) process, while silencing of miR-939-5p exhibited the opposite effect. However, when both LncRNA-LHX2 and miR-939-5p were simultaneously interfered with, the tumor growth\u0000 was partially alleviated. Based on these results, our study highlights the malignant impact of LncRNA-LHX2 in the progression of liver cancer, indicating its potential as a candidate biomarker for liver cancer diagnosis.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139824092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA-153 Restrains Cell Metastasis and Epithelial–Mesenchymal Transition in Cervical Carcinoma by Targeting SATB1 and Regulating Wnt/β-Catenin Pathway MicroRNA-153 通过靶向 SATB1 和调控 Wnt/β-Catenin 通路抑制宫颈癌的细胞转移和上皮-间质转化

IF 2.9 4区医学

Journal of biomedical nanotechnology Pub Date : 2024-02-01 DOI: 10.1166/jbn.2024.3761

Wenfeng Liu, Meng Zhang, Xiaojie Du, Min Zhang, Weiling Wang, Zhiying Zhang

引用次数: 0

The Protective Effect of Dihydroartemisinin on Type 2 Diabetic Mice via Regulating Hepatic Glucose Output 双氢青蒿素通过调节肝脏葡萄糖输出对 2 型糖尿病小鼠的保护作用

IF 2.9 4区医学

Journal of biomedical nanotechnology Pub Date : 2024-02-01 DOI: 10.1166/jbn.2024.3772

Yu Zhang, Yuan-min Yang, Shui-qing Qu, Shuo-qiu Deng, Yu Li, Zhongyuzn Zheng, Yue Dai, Tuo Liu, Li-na Chen, Yu-jie Li

{"title":"The Protective Effect of Dihydroartemisinin on Type 2 Diabetic Mice via Regulating Hepatic Glucose Output","authors":"Yu Zhang, Yuan-min Yang, Shui-qing Qu, Shuo-qiu Deng, Yu Li, Zhongyuzn Zheng, Yue Dai, Tuo Liu, Li-na Chen, Yu-jie Li","doi":"10.1166/jbn.2024.3772","DOIUrl":"https://doi.org/10.1166/jbn.2024.3772","url":null,"abstract":"There was an investigation into the hypoglycemic effects and potential mechanisms of dihydroartemisinin (DHA) on hepatic glycometabolism of type 2 diabetes mellitus (T2DM). The db/db mice and ApoE−/− mice induced by streptozotocin (STZ) were selected as diabetes\u0000 models. The levels of FBG, body weight, glucose tolerance, insulin, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were observed to evaluate the drug’s efficacy. The glycogen content, glucose-6-phosphate (G6P), hexokinase (HK) and glucose-6-phosphatase (G6pase)\u0000 were detected in the livers. Histopathological studies were conducted on the pancreas and liver. Targeting proteins and signaling pathways of DHA were identified by quantitative proteomic. Western blotting examined the protein expression of forkhead box protein O1 (FOXO1) and calcium/calmodulin-dependent-protein\u0000 kinase 2 (CAMK2) in the liver. This study demonstrated that DHA reduced FBG, improved insulin sensitivity, ameliorated glucose tolerance in two diabetes models while decreasing the ALT and AST levels in db/db mice. DHA promoted hepatic glucose metabolism and inhibited gluconeogenesis via CAMK2/FOXO1-mediated\u0000 HK upregulation and G6pase downregulation. In conclusion, DHA exerts protective effects against T2DM related to maintain the blance of hepatic glucose.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139827536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of HaCaT Proliferation and Imiquimod-Induced Psoriasis by Calcipotriol Through Regulation of the Glutathione/Glutathione Peroxidase 4 Pathway 卡泊三醇通过调节谷胱甘肽/谷胱甘肽过氧化物酶 4 通路抑制 HaCaT 增殖和咪喹莫特诱导的牛皮癣

IF 2.9 4区医学

Journal of biomedical nanotechnology Pub Date : 2024-02-01 DOI: 10.1166/jbn.2024.3777

Lei Yang, Yue Zhang, Jiansong Wu, Lei Wang, Shan Liu, Li Zhou, Jigang Zhang, Chengxin Li

{"title":"Inhibition of HaCaT Proliferation and Imiquimod-Induced Psoriasis by Calcipotriol Through Regulation of the Glutathione/Glutathione Peroxidase 4 Pathway","authors":"Lei Yang, Yue Zhang, Jiansong Wu, Lei Wang, Shan Liu, Li Zhou, Jigang Zhang, Chengxin Li","doi":"10.1166/jbn.2024.3777","DOIUrl":"https://doi.org/10.1166/jbn.2024.3777","url":null,"abstract":"Psoriasis is a chronic and recurrent skin disease characterized by aberrant proliferation and differentiation of keratinocyte cells. Although calcipotriol has been employed in the clinical management of psoriasis, no association between the anti-inflammatory mechanism and iron death\u0000 has been reported. Therefore, we assume that calcipotriol may down-regulate cell activity and suppress the expression of tissue inflammatory factors by regulating the glutathione (GSH) and glutathione peroxidase 4 (GPX4) pathway, thereby alleviating tissue inflammation and ameliorating psoriasis\u0000 symptoms. The experimental groups consisted of a control group, a model group, a Calcipotriol group, and a Calcipotriol+Ferrostatin-1 group. In vitro experiments, a lipopolysaccharides-induced HaCaT cell model was established. In vivo experiments, an imiquimod-induced psoriasis\u0000 mice model was constructed. The results showed that calcipotriol effectively downregulated the expression of GPX4 and GSH, thereby inhibiting HaCaT cell proliferation through modulation of Ki-67 protein expression and DNA breakage. Ferrostatin-1 could partially reverse these effects. Additionally,\u0000 calcipotriol downregulated the expression of GPX4 and GSH in skin tissues and upregulated the expression of long-chain acyl-CoA synthetase 4 by suppressing the levels of SLC7A11 and ferritin, leading to promote the accumulation of ROS and ferroptosis. Moreover, calcipotriol demonstrated inhibitory\u0000 effects on the inflammatory mediators and attenuated skin inflammation. Therefore, calcipotriol effectively ameliorated psoriatic lesions. In conclusion, this study revealed that calcipotriol exerts its therapeutic potential by promoting cellular clearance and suppressing tissue inflammation\u0000 through upregulation of ferroptosis progression. Therefore, this study provides new therapeutic drugs and functions for the treatment of psoriasis.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139872517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Protective Effect of Dihydroartemisinin on Type 2 Diabetic Mice via Regulating Hepatic Glucose Output 双氢青蒿素通过调节肝脏葡萄糖输出对 2 型糖尿病小鼠的保护作用

IF 2.9 4区医学

Journal of biomedical nanotechnology Pub Date : 2024-02-01 DOI: 10.1166/jbn.2024.3772

Yu Zhang, Yuan-min Yang, Shui-qing Qu, Shuo-qiu Deng, Yu Li, Zhongyuzn Zheng, Yue Dai, Tuo Liu, Li-na Chen, Yu-jie Li

{"title":"The Protective Effect of Dihydroartemisinin on Type 2 Diabetic Mice via Regulating Hepatic Glucose Output","authors":"Yu Zhang, Yuan-min Yang, Shui-qing Qu, Shuo-qiu Deng, Yu Li, Zhongyuzn Zheng, Yue Dai, Tuo Liu, Li-na Chen, Yu-jie Li","doi":"10.1166/jbn.2024.3772","DOIUrl":"https://doi.org/10.1166/jbn.2024.3772","url":null,"abstract":"There was an investigation into the hypoglycemic effects and potential mechanisms of dihydroartemisinin (DHA) on hepatic glycometabolism of type 2 diabetes mellitus (T2DM). The db/db mice and ApoE−/− mice induced by streptozotocin (STZ) were selected as diabetes\u0000 models. The levels of FBG, body weight, glucose tolerance, insulin, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were observed to evaluate the drug’s efficacy. The glycogen content, glucose-6-phosphate (G6P), hexokinase (HK) and glucose-6-phosphatase (G6pase)\u0000 were detected in the livers. Histopathological studies were conducted on the pancreas and liver. Targeting proteins and signaling pathways of DHA were identified by quantitative proteomic. Western blotting examined the protein expression of forkhead box protein O1 (FOXO1) and calcium/calmodulin-dependent-protein\u0000 kinase 2 (CAMK2) in the liver. This study demonstrated that DHA reduced FBG, improved insulin sensitivity, ameliorated glucose tolerance in two diabetes models while decreasing the ALT and AST levels in db/db mice. DHA promoted hepatic glucose metabolism and inhibited gluconeogenesis via CAMK2/FOXO1-mediated\u0000 HK upregulation and G6pase downregulation. In conclusion, DHA exerts protective effects against T2DM related to maintain the blance of hepatic glucose.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139887558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0