Journal of biomedical nanotechnology最新文献

{"title":"Multifunctional Nanoparticles Fabricated by Dispersion Polymerization for the Treatment of Triple-Negative Breast Cancer.","authors":"Onyinyechi Obidiro, Gantumur Battogtokh, Bharathi Mandala, Victor Ejigah, Emmanuel O Akala","doi":"10.1166/jbn.2026.3949","DOIUrl":"10.1166/jbn.2026.3949","url":null,"abstract":"<p><p>This work presented the design and development of pH-sensitive nanoparticles fabricated by dispersion polymerization (PEG-surface-tagged polymeric nanoparticles) loaded with cisplatin and paclitaxel. <i>In vitro</i> drug release and <i>in vitro</i> drug efficacy of the dual-loaded nanoparticles were carried out vis-à-vis their suitability for the treatment of triple-negative breast cancer (TNBC). A pH-sensitive acetal crosslinking agent was synthesized followed by incorporation in polylactide, poly(<i>ε</i>-caprolactone and polylactide-poly(<i>ε</i>-caprolactone) (blend) nanoparticles to facilitate the hydrolysis of the nanoparticle matrix in the tumor acidic microenvironment and the release of the encapsulated drugs. The efficacy of paclitaxel and cisplatin, when combined, and the nature of their interaction were determined based on a combination index in an <i>in vitro</i> cell-based assay (antiproliferative studies) using a triple-negative breast cancer cell line (MDA-MB-231). Data show a synergistic interaction between paclitaxel and cisplatin: the two drugs work together to enhance efficacy. Combination Index values for the different formulations were less than 1, showing synergism. The drug combination demonstrated greater cytotoxicity at lower doses compared to higher doses of paclitaxel or cisplatin alone, indicating that this dual-loaded drug approach could maintain therapeutic efficacy while reducing adverse side effects commonly associated with chemotherapy at high doses. This novel strategy not only facilitates dose reduction of chemotherapeutic agents but also enables the simultaneous, site-specific delivery of both drugs, offering a promising advancement in targeted TNBC therapy. Confocal microscopy studies showed the internalization of the nanoparticles in the cytoplasm.</p>","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"22 1","pages":"47-69"},"PeriodicalIF":2.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13384477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148603481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Levobupivacaine Hydrochloride-Loaded Nanospheres on Delayed Cerebral Vasospasm Following Subarachnoid Hemorrhage in Rabbits","authors":"Mengke Fang, Li Chen, Sheng-qiang Lu","doi":"10.1166/jbn.2024.3904","DOIUrl":"https://doi.org/10.1166/jbn.2024.3904","url":null,"abstract":"This research was aimed to analyze the mechanism of action of levobupivacaine hydrochloride-loaded nanospheres on delayed cerebral vasospasm following subarachnoid hemorrhage (SAH). Levobupivacaine hydrochloride-loaded nanospheres (LevoBPV Hcl/PLGA) were prepared using the solvent evaporation methodology, with the raw material as a control. The blood drug concentrations were detected by HPLC after subcutaneous and subarachnoid administration in experimental rabbits. Forty New Zealand white rabbits were randomly assigned into Sham group, SAH group, LevoBPV Hcl group (10 mg/kg), and LevoBPV Hcl/PLGA group (10 mg/kg), with 10 rabbits in each group. The SAH model was induced using the double blood injection methodology combined with internal carotid artery ligation. Brain tissue samples were collected on day 7 for pathological characterization, determination of neuronal apoptosis, and measurement of basilar artery diameter and area. The levels of oxidative stress factors (superoxide (SOD), malondiadehyde (MDA), glutathione peroxidase (GSH-Px)) and vasoconstrictor factors (nitric oxide (NO), endothelin-1 (ET-1)) in the cerebrospinal fluid (CSF) were detected using assay kits. The results revealed that the drug loading capacity of LevoBPV Hcl/PLGA was 29.13%, encapsulation efficiency was 87.09%, and the average particle size was 81.43 μ m. Under the same dosage, both subcutaneous and subarachnoid administration of LevoBPV Hcl/PLGA exhibited two concentration peaks in the blood drug concentration, with lower concentration values versus LevoBPV Hcl group, and a longer average residence time than LevoBPV Hcl group ( P &lt; 0.05). Relative to Sham group, SAH group exhibited decreased diameter and area of the basilar artery, reduced neuronal density, increased neuronal apoptosis rate, decreased levels of SOD, GSH-Px, and NO in the CSF, and increased levels of MDA and ET-1 ( P &lt; 0.05). Moreover, LevoBPV Hcl group and LevoBPV Hcl/PLGA group showed increased diameter and area of the basilar artery, higher neuronal density, reduced neuronal apoptosis rate, elevated levels of SOD, GSH-Px, and NO in the CSF, and decreased levels of MDA and ET-1 versus SAH group ( P &lt; 0.05). The LevoBPV Hcl/PLGA group exhibited increased diameter and area of the basilar artery, higher neuronal density, reduced neuronal apoptosis rate, elevated levels of SOD, GSH-Px, and NO in the CSF, and decreased levels of MDA and ET-1 versus LevoBPV Hcl group ( P &lt; 0.05). In short, LevoBPV HCl-loaded nanospheres can prolong the in vivo residence time of subcutaneous and subarachnoid administration, reduce the maximum blood drug concentration, and enhance drug safety. Furthermore, these nanospheres can inhibit neuronal apoptosis following SAH, regulate oxidative stress and vasoconstrictor factor expression, thereby suppressing the occurrence of delayed cerebral vasospasm and alleviating brain tissue damage.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"20 9","pages":"1483-1491"},"PeriodicalIF":0.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147922614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation, Pharmacokinetics and Anti-Liver Injury Pharmacodynamic Study of Esculin Loaded Liposomes Modified by TPGS","authors":"Feng Shi, Weigang Sun, Wenxiong Yin, Xiaoli Xia, Michael Adu‐Frimpong, Xia Cao, Hao Ji, Qilong Wang, Ximing Xu, Jiangnan Yu","doi":"10.1166/jbn.2024.3911","DOIUrl":"https://doi.org/10.1166/jbn.2024.3911","url":null,"abstract":"In this study, Esculin (ES)-loaded liposomes modified with TPGS (ES-TPGS-Ls) were successfully prepared to enhance bioavailability and hepatoprotective activity of this coumarin glucoside. We utilize thin-film dispersion to fabricate ES-TPGS-Ls. The size, polydispersed index (PDI), Zeta potential (Z-potential), morphology, and encapsulation effectiveness of the liposomes were all evaluated afterwards. The storage stability of ES-TPGS-Ls at 4 °C was investigated. The findings showed that ES-TPGS-Ls had spherical nanoparticles with 194.47±8.54 nm as the mean size, 0.239±0.011 as PDI, and −21.16±0.97) mV as Z-potential coupled with encapsulation efficiency (EE) of 91.85±0.44%. Storage stability of liposomes at 4 °C was maintained within one week. Pharmacokinetic study showed that the relative oral bioavailability of ES-TPGS-Ls increased by 2.38 times. Pharmacodynamic studies showed that the developed liposomes could enhance the hepatoprotective activity of ES. Overall, the ES-TPGS-Ls significantly enhanced the bioavailability of esculin, thereby enhancing the in vivo hepatoprotective effect of ES.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"20 9","pages":"1457-1466"},"PeriodicalIF":0.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147886016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role and Mechanism of circAMOTL1 in Hypertensive Heart Failure","authors":"Yanhong Mou, Xin Fan, Runmin Sun, Xiaowei Zhang, Jing Yu","doi":"10.1166/jbn.2024.3897","DOIUrl":"https://doi.org/10.1166/jbn.2024.3897","url":null,"abstract":"To explore the changes of circAMOTL1 expression, cell function and expression of fibrosis-related proteins in myocardial hypertrophy and fibrosis model. Human cardiomyocytes AC16 were cultured, and the concentration of Ang II was firstly explored by CCK-8. After determining the optimal dose of Ang II, AC16 cells were induced to construct an in vitro model of myocardial hypertrophy and fibrosis. CCK-8 was utilized to assess cell proliferation. Flow cytometry was performed to validate cell cycle and apoptosis. circAMOTL1, miR-330-3p, smad7, Col1a2 and Col3a1 genes expressions were assessed by qRT-PCR. smad7, Col1a2 and Col3a1 proteins expressions were evaluated using Western blot and IF. FISH was performed to detect circAMOTL1 localization in cells. 10 μ M Ang II was selected for subsequent experiments. Compared with control group, cell viability of the Ang II group was significantly decreased, apoptosis was observably increased, circAMOTL1 and smad7 expressions were signally repressed, miR-330-3p, Col1a2 and Col3a1 expressions were notably increased. Both circAMOTL1 and miR-330-3p, miR-330-3p and smad7 had targeted binding sites. Overexpression of circAMOTL1 promoted AC16 cells proliferation and inhibited apoptosis. In addition, overexpression of circAMOTL1 inhibited miR-330-3p and promoted smad7 expression. Overexpression of circAMOTL1 inhibited miR-330-3p and promoted smad7 expression. circAMOTL1 may be a potential target for treating hypertensive heart failure.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"20 9","pages":"1519-1526"},"PeriodicalIF":0.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Petasitin as Natural Extract on Proliferation and Pathological Changes of Pediatric Neuroblastoma SK-N-SH Cells","authors":"Ling Li, Mingjun Zheng, Weixia Meng","doi":"10.1166/jbn.2024.3902","DOIUrl":"https://doi.org/10.1166/jbn.2024.3902","url":null,"abstract":"This research was developed to investigate the impact and mechanisms of petasitin as natural extract on the proliferation and pathological changes of pediatric neuroblastoma SK-N-SH cells. The sample cells were selected as experimental materials and randomly rolled into a control (Ctrl), a low-dose (LD), a medium-dose (MD), and a high-dose (HD) group, which were subjected to regular culture, 0.5, 1.5, and 5 μ M/L petasitin for 12 hours, respectively. Meanwhile, the cell proliferation was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Flow cytometry (FCT) was employed to assess the cycle progression and apoptosis of SK-N-SH cells, while Hoechst 33258 fluorescent staining was applied to observe the nuclear changes. Meanwhile, the real-time quantitative PCR (RT-qPCR) was applied to measure the mRNA levels of PI3K, PTEN, AKT1, and mTOR genes in SK-N-SH cells. Western blot was conducted to determine the protein expression levels (PELs) of p-PI3K, PI3K, p-Akt, Akt, p-mTOR, and mTOR in SK-N-SH cells. The results revealed that after treatment for 48 and 72 hours, the optical density (OD) values in the petasitin treatment groups were lower and exhibited great differences to those in the Ctrl group ( P &lt; 0.05), while inhibition rates (IRs) were higher ( P &lt; 0.05). Furthermore, the petasitin treatment groups exhibited an obvious increase in the G1/G0 cell cycle ratio (CCR) and a great decrease in S and G2/M phase CCR ( P &lt; 0.05). Moreover, the apoptotic rates in the petasitin treatment groups were much higher at different time points, showing observable differences with P &lt; 0.05. Besides, the Hoechst 33258 staining positivity rates (PRs) of SK-N-SH cell nuclei in the petasitin treatment groups were higher and presented great differences with P &lt; 0.05 to those in the Ctrl group. In addition, the cells in petasitin treatment groups exhibited greatly downshifted mRNA levels of PI3K and AKT1 and obviously elevated PTEN (all P &lt; 0.05). PELs of PI3K, AKT1, and mTOR exhibited no great differences ( P &gt; 0.05), but phosphorylation levels of p-PI3K, p-Akt, and p-mTOR were decreased in the petasitin treatment groups, presenting great differences with P &lt; 0.05. Together, these results suggested that petasitin exerted a suppressive role in proliferation and promote the apoptosis of pediatric SK-N-SH cells by adjusting PI3K-Akt-mTOR signaling pathway (SPW).","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"20 9","pages":"1441-1449"},"PeriodicalIF":0.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147888406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune Clearance Mechanism for Hypoxia-Inducible Factor in Pelvic Peritonitis by Directly Targeting MiR-206a Using Magnetic Ferric Oxide Particles as a Carrier","authors":"Qing Li, Dianfang Liu, Yanhong Zhang","doi":"10.1166/jbn.2024.3873","DOIUrl":"https://doi.org/10.1166/jbn.2024.3873","url":null,"abstract":"miR-206a can directly target and regulate hypoxia-inducible factor, playing a role in inflammation and immune response. This study explored the effect of miR-206a using magnetic ferric oxide particles as a carrier to directly target and regulate HIF on immune clearance mechanism for pelvic peritonitis. A rat model of pelvic peritonitis was constructed and divided into NC group, NC group, Fe 3 O 4 nanoparticle group, Fe 3 O 4 -miR-206a group, miR-206a gene knockout group, 740Y-P group, BKM120 group, Fe 3 O 4 -miR-206a+BKM120 group, PHD group, BAY 87-2243 group, Fe 3 O 4 -miR-206a+PHD group, 740Y-P+PHD group, BKM120+PHD group, and Fe 3 O 4 -miR-206a+BKM120+PHD group. We then detected serum C-Reactive protein (CRP), TNF- α and IL-6 levels, T lymphocyte, and miR-206a expression. HIF, TNF, IL-1, and IL-6 protein expressions were also detected. A rat model for pelvic peritonitis was also established, and magnetic ferric oxide particles carrying miR-206a nanocomplexes were successfully prepared. Levels of TNF- α , IL-6 and CRP were reduced under intervention by Fe 3 O 4 -miR-206a, and blood flow was reduced. All chemical indicators were reduced, and inflammatory response was significantly improved. After knocking out the miR-206a, the opposite results were observed. This study findings show that, miR-206a can directly target and regulate HIF to inhibit PI3K/Akt signaling pathway using magnetic ferric oxide particles as a carrier, thereby improving the immune clearance ability for patients with pelvic peritonitis, reducing inflammatory reactions, and thus improving prognosis. In addition, this research not only provides a new direction for management of inflammatory diseases, but also serves as a reference for treatment of immune diseases. Therefore, direct targeting and regulating HIF by miR-206a using magnetic ferric oxide particles as a carrier can become an effective strategy for the treatment of inflammatory and immune-related diseases.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"20 8","pages":"1287-1295"},"PeriodicalIF":0.0,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147916815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"M1 Macrophage-Derived Exosomal MiR-155 Enhances Autophagy in Sepsis-Triggered Acute Kidney Injury","authors":"Yunhan Zhu, Renshou Chen","doi":"10.1166/jbn.2024.3849","DOIUrl":"https://doi.org/10.1166/jbn.2024.3849","url":null,"abstract":"Sepsis-induced Acute kidney injury (SA-AKI), a common complication in sepsis, significantly impacts patients’ health and quality of life. M1 macrophages have been demonstrated to release inflammatory mediators that exacerbate kidney injury. MiR-155 has been implicated in promoting inflammation and damage during sepsis while reducing miR-155 levels alleviates SA-AKI. However, the relationship between miR-155 and M1 macrophage-derived exosomes in regulating autophagy during SA-AKI remains unclear. In this study, we aim to investigate the relationship between M1 macrophage-derived exosomes and miR-155 in regulating autophagy during SA-AKI. A mouse model of SA-AKI was established by performing cecal ligation and puncture (CLP) surgery. Additionally, the HK-2 cell line was utilized to establish a sepsis cell model by inducing lipopolysaccharide (LPS). We demonstrated that the mice model of SA-AKI exhibited renal injury along with enhanced autophagy, inflammation response, and macrophage polarization after CLP surgery. M1 macrophages attenuated cell viability and enhanced autophagy in LPS-treated HK-2 cells. Additionally, M1 macrophage-derived exosomes were observed to enhance autophagy in LPS-treated HK-2 cells. Furthermore, we confirmed an increased expression of miR-155 in M1 macrophage-derived exosomes. Furthermore, exosome-mediated miR-155 enhanced autophagy in LPS-treated HK-2 cells. In conclusion, this study provides the first evidence that exosomal miR-155 derived from M1 macrophages enhances autophagy in SA-AKI. These findings suggest that targeting exosomal miR-155 could be a promising therapeutic strategy for SA-AKI.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"20 8","pages":"1307-1314"},"PeriodicalIF":0.0,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147916700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circ_0060060 Accelerates Metastasis of Non-Small-Cell Lung Carcinoma by Upregulating FUT8","authors":"Yue Huo, Lin An, Qi Zhao, Na Li, Zhen He, Kunjie Wang","doi":"10.1166/jbn.2024.3875","DOIUrl":"https://doi.org/10.1166/jbn.2024.3875","url":null,"abstract":"We aimed to elucidate the diagnostic and prognostic values of Circ_0060060 in non-small-cell lung cancer (NSCLC), and its regulatory effect on FUT8. Relative levels of Circ_0060060 and FUT8 were examined in NSCLC and adjacent tissues. Clinical information of recruited NSCLC patients was collected for assessing the prognostic value of Circ_0060060. Regulatory effects of Circ_0060060 and FUT8 on in vitro proliferative and migratory abilities of NSCLC were examined by cell counting kit-8 (CCK-8) and transwell assay, respectively. Finally, the interaction between Circ_0060060 and FUT8 was determined via dual-luciferase reporter assay and Pearson correlation test. Circ_0060060 and FUT8 were upregulated in NSCLC tissues. High level of Circ_0060060 or FUT8 predicted higher incidences of distant metastasis and worse overall survival in NSCLC patients. in vitro evidences have shown the roles of Circ_0060060 and FUT8 in stimulating proliferative and migratory abilities of PC-9 and SPC-A1 cells. Circ_0060060 directly targeted FUT8 and positively regulated its level. Circ_0060060 and FUT8 are effective diagnostic markers of NSCLC. Circ_0060060 stimulates proliferative and migratory abilities of NSCLC by regulating FUT8.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"20 7","pages":"1131-1137"},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147910228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blockade of Cluster of Differentiation 38 Alleviates Sepsis-Induced Acute Lung Injury in Mice Through Inflammation Suppression via Regulating Toll-Like Receptor 4/Nuclear Factor kappa B p65","authors":"Xian‐Jin Zhang, Hui Shen, Li Wang, Miaomiao Zhu, Yingzhi Wang","doi":"10.1166/jbn.2024.3869","DOIUrl":"https://doi.org/10.1166/jbn.2024.3869","url":null,"abstract":"This study aimed to investigate the role of CD38 inhibition in sepsis-induced acute lung injury (ALI) using a murine model. ALI, commonly associated with excessive inflammation, was induced by cecal ligation and puncture (CLP). We observed an increase in CD38 expression in lung tissue over time in the ALI mice. To address this, we administered the CD38 inhibitor, 78C, subcutaneously at a dose of 10 mg/kg. Following treatment, we assessed lung function, inflammatory cell levels, and cytokine concentrations in bronchoalveolar lavage fluid. We also examined the activity of the TLR4/NF- κ B p65 signaling pathway in lung tissue. Results showed that 78C treatment improved lung function and reduced inflammatory cells and cytokines in ALI mice. Moreover, 78C inhibited the expression of the TLR4/NF- κ B p65 signaling pathway in lung tissue. This CD38 blockade effectively mitigated inflammation levels in lung tissue, ameliorated lung function, and alleviated sepsis-induced ALI induced by CLP. These findings suggest that targeting CD38 to modulate the TLR4/NF- κ B p65 inflammatory pathway holds promise as a therapeutic strategy for sepsis-induced ALI.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"20 7","pages":"1161-1168"},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147894251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stanniocalcin-2: A Potential Predictor of Residual Breast Cancer After Neoadjuvant Chemotherapy","authors":"Yuan Li, Xiangyi Kong, Changyuan Guo, Lei Guo, Chuqi Lei, Ke Zhang, Zhongzhao Wang, Jidong Gao, Lixue Xuan","doi":"10.1166/jbn.2024.3881","DOIUrl":"https://doi.org/10.1166/jbn.2024.3881","url":null,"abstract":"Compared to patients of pathological complete response (pCR), patients of residual lesion afterwards neoadjuvant chemotherapy (NAC) have worse prognosis and higher distant metastasis. Irrespective, there exists limited data on effective indicators and treatment methods for these patients. In our study, we evaluated the relationship between stanniocalcin-2 (STC2) marker and the prognosis of breast cancer patients of residual lesion after receiving NAC. The relationship between STC2 and patients’ prognosis was evaluated by transcriptome analysis. The impact of the level of STC2 on cell migration, invasion, and proliferation was evaluated at the cellular level. Immunohistochemistry was performed on 293 patients with residual disease after NAC to assess STC2 protein levels. The relationship between STC2 and survival rate was estimated using Cox regression model and Kaplan Meier analysis. The results demonstrated that high level of STC2 significantly inhibited migration, invasion, and proliferation of breast cancer cells. Compared to patients with low STC2, high STC2 were directly proportional to overall survival (OS) and disease-free survival (DFS) (OS: 86.96 vs. 84.62 months, P = 0.017 DFS: 77.33 vs. 66.95 months, P -value &lt; 0.001). In conclusion, our research suggested that the of level STC2 may be a promising prognostic marker or therapeutic targe for breast cancer patients with residual lesion after NAC.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"20 7","pages":"1106-1118"},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147883072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}