{"title":"Influence of reproductive cycle, sex, age and season on haematologic parameters in domestic animals: A review","authors":"Yaqub, Kawu, Ayo","doi":"10.5897/JCAB12.063","DOIUrl":"https://doi.org/10.5897/JCAB12.063","url":null,"abstract":"Haematologic parameters play a pivotal role in clinical diagnosis, the evaluation of patient before surgical intervention and in monitoring responses to therapy. The paper reviews haematologic parameters as influenced by exogenous and endogenous factors, including reproductive cycle, sex,age and season, with emphasis on domestic animals reared in the tropics. It is concluded that reproductive cycle, sex, age and season modulate haematologic parameters, and that they should be considered in order to ensure accurate interpretation of the parameters in domestic animals. \u0000 \u0000 \u0000 \u0000 Key words: Haematology, reproductive cycle, age, sex and season.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"41 5 1","pages":"37-43"},"PeriodicalIF":0.0,"publicationDate":"2013-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89539907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Department of Biological Sciences, Covenant University, Ota, Ogun State, Nigeria.","authors":"Ogundele, J. Sanya, Madukwe, Caxton-Martins","doi":"10.5897/JCAB12.044","DOIUrl":"https://doi.org/10.5897/JCAB12.044","url":null,"abstract":"One of the most prominent disease conditions in cassava endemic regions of the world is movement disorders. In this study, we investigate the movement disorder from the cortical and cerebellar point of view. Most toxicity studies involving movement disorder has been greatly linked to the motor cortex, thus, we examined neurodegeneration both in the motor cortex and the cerebellar cortex. This study also evaluated the possible role of such degeneration in the etiology of neurodegenerative diseases associated with cassava endemicity. 15 F1 adult Wistar rats were divided into three groups of five animals each. The first group was the control, the second group received 10 mg/kg BW of potassium cyanide (KCN) and the third group received 20 mg/kg BW of KCN for 15 days. The cortical (motor area) and cerebellar tissue were obtained and fixed in formol calcium for cyto-architectural study. In conclusion, toxicity of cyanide in the cortex and cerebellum can involve osmotic imbalance and excitotoxicity at the 10 mg/kg causing increased in cell size and a slower form of degeneration and reactive oxygen species (ROS) generation and lipid peroxidation causing release hydrolytic enzymes from lysosomes that destroys the components of the cytoplasm as observed in the 20 mg/Kg treatment. \u0000 \u0000 \u0000 \u0000 Key words: Neuron, cell death, cerebellum, cortex, movement, degeneration, oxidative stress.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"5 1","pages":"31-36"},"PeriodicalIF":0.0,"publicationDate":"2013-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79927148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of sucrose and propylene glycol on the vitrification of sheep oocytes","authors":"I. Dike, O. Obembe","doi":"10.5897/JCAB12.033","DOIUrl":"https://doi.org/10.5897/JCAB12.033","url":null,"abstract":"Vitrification solutions containing both permeating and non-permeating cryoprotectants provide better productions of oocytes during freezing and thus contribute to the survival of oocytes. We have investigated the efficency of sucrose in combination with propylene glycol (PROH) on the cryopreservation of the oocytes at the meoisis II (MII) stage. The vitrification of sheep oocytes was performed using 5 M PROH, PROH in combination with sucrose at varying concentrations (5 M PROH + 0.5 M sucrose/1 M sucrose/1.5 M sucrose/2 M sucrose) and 1.0 M sucrose. It was concluded that sucrose in combination with propylene gylcol is most efficient at concentration between 1.0 to 1.5 M. Sucrose was found to aid in the maintainance of the morphological structure of the oocytes vitrified but at high concentrations sucrose has adverse effect on cleavage. Hence sucrose is best used as an additive cryoprotectant when supporting a permeating cryoprotectant, which maintains the integirty of the internal structures through its permeability.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"64 1","pages":"25-30"},"PeriodicalIF":0.0,"publicationDate":"2013-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72601256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of dietary prebiotic and acidifier supplementation on the growth performance, carcass characteristics and serum biochemical parameters of broilers","authors":"R. Fallah, H. Rezaei","doi":"10.5897/JCAB12.065","DOIUrl":"https://doi.org/10.5897/JCAB12.065","url":null,"abstract":"This study investigated the effect of dietary supplementation with the fermacto prebiotic and Biotronic®S.E (an acidifier) on the growth performance, carcass characteristics and serum biochemical parameters of broiler chickens. 240 day old Ross 308 broilers were equally distributed into 24 floor pens and reared for 42 days. A basal diet was formulated according to the recommendations of NRC for starter (1 to 21 days) and grower (22 to 42 days) periods and considered as control diet. Four tested diets were formulated as listed control diet (without any additives), fermacto prebiotic, Biotronic®S.E (an acidifier) and mixture of fermacto prebiotic plus Biotronic® S.E. Six replicate were used for each treatment. The results of this study indicate that addition of fermacto prebiotic and Biotronic® S.E increased the final body weight, significantly (p˂0.05). Furthermore, addition of additives decreased abdominal fat, triglycerides, serum cholesterol and increased carcass weight. Addition of fermacto prebiotic plus Biotronic® S.E had the best performance, carcass characteristics and lowest serum cholesterol on broiler chickens at 42 day of age. \u0000 \u0000 \u0000 \u0000 Key words: Fermacto prebiotic, Biotronic® S.E, performance, broiler, carcass characteristics, serum cholesterol.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"1 1","pages":"21-24"},"PeriodicalIF":0.0,"publicationDate":"2013-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82631979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Olfati, G. Moghaddam, H. D. Kia, H. K. Shabankareh
{"title":"Effects of prostaglandin F 2 α treatment on semen characteristics of crossbred rams in the non-breeding season","authors":"A. Olfati, G. Moghaddam, H. D. Kia, H. K. Shabankareh","doi":"10.5897/JCAB2013.0204","DOIUrl":"https://doi.org/10.5897/JCAB2013.0204","url":null,"abstract":"The aim of the present study was to evaluate the effects of administration of PGF2α on semen characteristics in crossbred rams in the non-breeding season. Twenty crossbred rams (Arkhar-Merino × Moghani and Arkhar-Merino × Ghezel) were randomly allocated into two equal groups. The experimental group received 7.5 mg IM of Estroplan (PGF2α analogue) and the control group received 1 ml of water soluble. A total number of eighteen ejaculates per ram were collected by artificial vagina twice a week 30 min following IM administration. Semen ejaculates were evaluated for volume, sperm concentration, total sperm output, mass and individual motility, methylene blue reduction time-test (MBRT), percentage of live sperm and sperm abnormality rates. The results of the current study shows that PGF2α treatment on crossbreed rams improved most semen characteristics including volume, sperm concentration, total sperm output and MBRT in comparison to control group (P < 0.01). However, other semen characteristics show similar values. We concluded that PGF2α actually improved semen output without any negative effect on sperm qualities.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"40 1","pages":"16-20"},"PeriodicalIF":0.0,"publicationDate":"2013-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77715668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of molasses level in a concentrate mixture on performances of crossbred heifer calves fed a basal diet of maize stover","authors":"D. Assefa, A. Nurfeta, I. Banerjee","doi":"10.5897/JCAB12.054","DOIUrl":"https://doi.org/10.5897/JCAB12.054","url":null,"abstract":"This study was conducted to evaluate the growth performance and feed intake of crossbred (Boran x Holstein Friesian) female calves fed different levels of molasses in concentrate mixture using 24 yearlings calves with average initial weight of 142.4±20.7 kg which lasted for 90 days. The calves were assigned into treatments having 0 (T1), 15 (T2), 30 (T3) and 50% (T4) molasses which replaced wheat bran in concentrate mixture using randomized complete block design into six blocks of four animals. The dry matter (DM) degradability was determined by incubating 3 g of feed samples in fistulated steers fed natural pasture hay ad libitum supplemented with 2 kg of concentrate. The total DM and organic matter (OM) intake for T2 and T3 diets were higher (P< 0.05) than those fed T1 and T4 diets. The stover DM and OM intake for T2 and T3 diets were higher (p<0.05) than for other treatments. The highest (p<0.05) crude protein intake was observed in calves fed T3 diets. Metabolizable energy (ME) intake was higher (P<0.05) for calves fed on T2 and T3 diets, respectively. Calves fed on T2, T3 and T4 diets had higher average daily gain compared to those fed T1 diet. The DM degradability after 4, 8, 24, 48, and 96 h of incubation was higher (P < 0.05) for T4 than that of T1. Based on intake of DM, OM and ME and growth performance, 15 and 30% molasses could be used as a replacement to wheat bran in the ration of heifers fed maize stover with good performance. \u0000 \u0000 \u0000 \u0000 Key words: Molasses, performance, crossbred, calves, maize stover, Ethiopia.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"19 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2013-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81930376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genetic analyses of first lactation traits of Boran and their crosses with Holstein and Jersey in central highlands of Ethiopia","authors":"B. Belay, A. Chakravarty","doi":"10.5897/JCAB12.059","DOIUrl":"https://doi.org/10.5897/JCAB12.059","url":null,"abstract":"Data obtained from Holeta Agricultural Research Center in Ethiopia were analyzed to compare the Boran cattle and the crossbreds and estimate genetic and crossbreeding parameters for first lactation traits. First lactation traits studied were first lactation total milk yield (FLMY), first lactation length (FLL), milk yield per day to first lactation (FLDMY), first 305 days milk yield (MY305) and adjusted first lactation 305 days milk yield (AFL305DMY). The effect of genetic group was significant (p 0.05) difference between HF x Bo and HF x HFBo or J x Bo and J x JBo and thus exotic inheritance beyond 50% showed no improvement in first lactation traits. The heritability estimates were medium (0.21+03) for FLL to high (0.39+0.40) for FLDMY. The breed additive difference for HF or Jersey was positive and significant (p 0.05) for Jersey x Boran crosses except FLDMY and AFL305DMY. Crossbreeding improved first lactation traits three to four folds. Thus, crossbreeding with management interventions could be recommended to improve milk production. \u0000 \u0000 \u0000 \u0000 Key words: Crossbreeding, genetic parameters, milk yield, non-genetic factors.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"15 1","pages":"9-15"},"PeriodicalIF":0.0,"publicationDate":"2013-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81963318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolation and Structure Elucidation of Acetyl Cholinesterase Inhibitor from Gyrostoma helianthus of the Red Sea, Egypt","authors":"M. Gomaa, A. Farag, A. Ayesh, M. A. Embaby","doi":"10.5897/JCAB2015.0431","DOIUrl":"https://doi.org/10.5897/JCAB2015.0431","url":null,"abstract":"4 Abstract: The present study was carried out to isolate, purify and elucidate the structure of low molecular weight bioactive compounds from sea anemone, Gyrostoma helianthus of the Red Sea environment. The obtained results indicated that the ethanolic crude extract of the sea anemone, Gyrostoma helianthus showed inhibition activity against acetylcholinesterase (AchE). The 0.5kD fraction inhibited the activity of AchE that indicated the presence of low molecular weight active compound(s) of less than 0.5kD. Two active fractions were obtained after BioGel P2 fractionation of the 0.5kD of the Gyrostoma helianthus. The first active fraction inhibited the activity of the acetylcholinesterase, while the other fraction was not. HPLC technique aided by semipreparative columns was used to isolate the target compounds in a pure form for the structure elucidation, one dimensional NMR analysis ( H and C-NMR) and DEPT were carried out to elucidate the structure of the 1 13","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"16 1","pages":"16-25"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88037967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of lead on gill and liver of blue spotted ray (Dasyatis kuhlii)","authors":"J. Tresnati, Iqbal Djawad","doi":"10.5897/JCAB12.035","DOIUrl":"https://doi.org/10.5897/JCAB12.035","url":null,"abstract":"The aim of study was to analyze the effect of lead nitrate Pb(NO3)2 on gill and liver of blue spotted ray Dasyatis kuhlii using a histological observation. The rays were placed in five tanks filled by seawater with three fishes for each tank. Blue spotted rays, Dasyatis kuhlii were treated for 12 days by using different concentrations of lead nitrate (Pb(NO3)2), that is, 0 ppm as control (treatment A), 0.2 ppm (treatment B), 0.5 (treatment C), 0.9 ppm (treatment D) and 1.8 ppm (treatment E). The rays were placed in the tanks until all fishes exposed to Pb (treatments B to E) were dead. Results of the study show that Pb(NO3)2 exposure altered the gill and liver microscopic structure. Gill lamella alterations were found on hypertrophy, hyperplasia, club shaped, necrosis and formed bunch texture. Liver alterations included cloudy swelling, atrophy, necrosis, vacuolar degeneration and fatty degeneration. Death time of blue spotted ray became shorter when lead nitrate Pb(NO3)2 concentration increased.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"141 1","pages":"250-256"},"PeriodicalIF":0.0,"publicationDate":"2012-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86784035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. S. Kohbanani, M. Nikravesh, M. Jalali, A. Fazel, M. Sankian
{"title":"Maternal nicotine exposure altered expression of laminin α 5 in lung tissue newborn mice","authors":"M. S. Kohbanani, M. Nikravesh, M. Jalali, A. Fazel, M. Sankian","doi":"10.5897/JCAB12.042","DOIUrl":"https://doi.org/10.5897/JCAB12.042","url":null,"abstract":"Maternal smoking has been clearly demonstrated to be associated with increased health problems in infants and children. Nicotine is a chemical substance with high level of toxicity. It crosses through the placenta and accumulates in the developing organs of fetus. Previous investigation indicated that maternal nicotine exposures induce decreased fibronectin expression in lung parenchyma. In this study, the effect of maternal nicotine exposure on laminin expression of the newborn mice lungs has been evaluated. 24 female pregnant Balb/C mice were divided randomly into four groups as follows: Experimental Group 1 (Exp D1); received 3 mg/kg nicotine intra peritoneal injection (IP) from gestational day 7 (GD7) to the last day of pregnancy, Experimental Group 2 (Exp D14); received 3 mg/kg nicotine from GD7 to post natal day 14, Groups 3 and 4; as sham control groups (Sha-Con) received the same volume (3 mg/kg) of normal saline parallel to experimental groups. At the end of exposure times, all the newborns were anesthetized, their lungs were removed and prepared for immunohistochemical method and real-time polymerase chain reaction. Our finding indicated that laminin alpha 5(Lama5) mRNA expression in the lung of newborn in the nicotine treated Exp D1 decreased by 0.63 fold but increased in Exp D14 by 1.57 fold comparing to Sh-Con groups. Lama5 immunoreactivity was not similar in different parts of the lungs including alveoli and bronchiole, having a significant increase in the experimental groups in contrast to the Sh-Con groups. These data also indicate that maternal nicotine exposure may induce abnormal laminin expression which may cause defects in lung function during life time.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"34 1","pages":"226-232"},"PeriodicalIF":0.0,"publicationDate":"2012-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73959517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}