A. Nadim, Pitu Flaviu, Pitu Florina, Popa Calin, E. Páll
{"title":"Effect of aqueous ozone solution on pancreatic cells","authors":"A. Nadim, Pitu Flaviu, Pitu Florina, Popa Calin, E. Páll","doi":"10.5897/JCAB11.079","DOIUrl":"https://doi.org/10.5897/JCAB11.079","url":null,"abstract":"The aim of this study was to evaluate the effects of ozone solutions on the viability of pancreatic cells. The ozone was generated by an electrical discharge on high-purity oxygen molecules. Ozonation of the water was performed by bubbling ozone through sterile distilled water (O3 concentration 4 g/1000 L) and saline solution (O3 concentration 5 g/1000 L). After initial dilution of test solutions, serial dilutions were made. In total, seven were tested for both types of solutions (0.00312 mg O3/1000 L, 0.00625 mg O3/1000 L, 0.0125 mg O3/1000 L, 0.025 mg O3/1000 L, 0.05 mg O3/1000 L, 0.1 mg O3/1000 L and 0.2 mg O3/1000 L). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra-zoliumbromide (MTT) cytotoxicity test was performed in triplicate after 24 h of treatment.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84260165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Application of premetamorphic oral cavity electron micrographs for Egyptian toads' taxonomy","authors":"G. Bekhet","doi":"10.5897/JCAB11.095","DOIUrl":"https://doi.org/10.5897/JCAB11.095","url":null,"abstract":"In the present study, the microanatomy of both the oral disc and buccal cavity of the tadpole of Bufo regularis was described. Tadpoles of 32, 38 and 40 stages were dissected and analyzed using scanning electron microscope. In all the stages, the mouth was ventral and the oral disk width was large, that is, equal to about 44% of the greatest width of the body. The disk was provided with a broad gap on the lower lip; the rest of the mouth was bordered by a large number of papillae. The papillae were arranged in a single row on the dorsolateral part of the mouth; the ventrolateral and ventral lip was surrounded by a double row of papillae. The number of papillae increased with larvae growth, from zero in stage 32 to about 150 in stage 40. The tooth row formula is 2(1)/3(2). The upper and lower beaks were pigmented and serrated. While the upper beak was broadly arched and formed a smooth arc, the lower beak had Vshape. Premetamorphic papillae were observed during the early metamorphic stages, and these degenerated rapidly at about late metamorphic stage. Metamorphic atrophy of the oral structures occurred roughly in the reverse order of development, although the procedure was rapid and more haphazard than the development. We suggested that the oral flaps and the roof papillae play a significant role in the capture of food particles by establishing the inflow of \"alimentary water\", and aggregating food particles and mucus inside the buccopharyngeal cavity, which may reflect ecological and functional constraints that are relative to the morphology of other suspension feeding anuran larvae. Herein, we described the oral features of the tadpoles of B. regularis.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"92 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90394210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of feed and water deprivation on diurnal variations in rectal temperature, respiration and heart rate of Yankasa sheep during the rainy season","authors":"J. Ayo, L. Yaqub, T. Dzenda","doi":"10.5897/JCAB.9000060","DOIUrl":"https://doi.org/10.5897/JCAB.9000060","url":null,"abstract":"Experiments were performed with the aim of determining diurnal fluctuations in rectal temperature (RT),respiratory rate (RR) and heart rate (HR) of Yankasa sheep, and the effect of feed and water deprivation on the physiological parameters. On the experimental day, seven experimental sheep were deprived of feed and water for 12 h, while four control sheep were given access to feed and water ad libitum. The RT, RR and HR of all the sheep were measured using standard procedures. The RT rose gradually from its minimum values of 39.0 ± 0.3 and 39.1 ± 0.2 0C at 07:00 h in experimental and control sheep, respectively, and attained peak values of 39.5 ± 0.3 and 39.7 ± 0.2 0C at 18:00 hours in the experimental and control animals, respectively. There was a significant (P < 0.05) and positive correlation between hour of the day and RT values in experimental and control sheep (r = 0.640 and 0.769, respectively). The RR and HR values of experimental sheep (30.1 ± 0.5 breaths/min and 88.6 ± 1.3 beats/min, respectively) were not significantly different from those of control sheep; 30.5 ± 0.8 breaths/min and 89.2 ± 1.6 beats/min, respectively. In conclusion, meteorological parameters, feed and water deprivation exerted significant influence on RT, RR and HR in Yankasa sheep, and they should be considered whenever the physiological parameters are measured in the breed, even during the rainy season. \u0000 \u0000 \u0000 \u0000 Key words: Diurnal fluctuations, rectal temperature, heart rate and respiratory rate.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"19 1","pages":"316-319"},"PeriodicalIF":0.0,"publicationDate":"2011-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82501628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Factors affecting conception rate in artificially inseminated cattle under farmers condition in Ethiopia","authors":"T. Woldu, Y. T. Giorgis, A. Haile","doi":"10.5897/JCAB11.067","DOIUrl":"https://doi.org/10.5897/JCAB11.067","url":null,"abstract":"This study was conducted in urban and rural areas of Adami Tullu district with objectives of evaluating factors affecting the efficiency of AI under farmers' situation and suggests options to be considered for improving the efficiency of AI delivery system. The overall conception rate attained in the study was 48.30% which is better than the national average conception rate reported so far. It was found out that the proportion of cows that successfully conceived significantly differed (P 0.05) and the pregnancy rate was higher in supplemented than in control cows (42.9 vs 25%; P>0.05). \u0000 \u0000 \u0000 \u0000 Key words: Artificial insemination, parity, breed, conception rate, heat detection.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"341 1","pages":"334-338"},"PeriodicalIF":0.0,"publicationDate":"2011-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76388584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Papatsiros, E. Tzika, P. Tassis, D. Kantas, L. Filippopoulos, D. S. Papaioannou
{"title":"Greek experience of the use of phytogenic feed additives in organic pig farming","authors":"V. Papatsiros, E. Tzika, P. Tassis, D. Kantas, L. Filippopoulos, D. S. Papaioannou","doi":"10.5897/JCAB11.070","DOIUrl":"https://doi.org/10.5897/JCAB11.070","url":null,"abstract":"1 Clinic of Medicine, Faculty of Veterinary Medicine, University of Thessaly, 43100, Karditsa, Greece. 2 Farm Animal Clinic, School of Veterinary Medicine, Aristotle University of Thessaloniki, Greece. 3 Department of Animal Production, Technological Educational Institute of Larissa, Greece. 4 Research Associate Technical Manager, PROVET, Vrago, Aspropyrgos, Attica Greece. 5 Department of Obstetrics and Reproduction, Faculty of Veterinary Science, University of Thessaly, Greece.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":" 944","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91409384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of supplemental chromium-methionine on reproductive performance of dairy cows in transition period","authors":"M. Khalili","doi":"10.5897/JCAB11.052","DOIUrl":"https://doi.org/10.5897/JCAB11.052","url":null,"abstract":"This experiment was conducted to study the effects of supplemental chromium on the reproductive performance of dairy cows. Thus, twenty multiparous Holstein cows (parity 3) were allocated to two treatments groups with ten replicates in a completely randomized design. In this study, the treatment consists of control group which received no chromium supplementation and treatment group which received 5 g/day of chromium from chromium methionine. The cows used for this experiment were within the range of 5 weeks prior to parturition to 12 weeks after parturition. Reproduction parameters consisting of insemination index and numbers of open days, clinical metabolic disorders which included acidosis, milk fever, retained placenta and displaced abomasums, and also clinical puerperal complications consisting of mastitis, endometritis and ovarian cysts, were determined. The results indicated that chromium (Cr) supplementation significantly caused a decrease in the numbers of open days (p < 0.05). Clinical metabolic disorders and clinical puerperal complications were not affected by chromium methionine supplementation. The results of this experiment also showed that chromium methionine supplementation in multiparous dairy cows diet may improve their milk yield and reproductive performance in transition period. \u0000 \u0000 \u0000 \u0000 Key words: Dairy cow, transition period, chromium methionine, reproduction.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"1 1","pages":"339-343"},"PeriodicalIF":0.0,"publicationDate":"2011-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88687276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Assessment of productive and reproductive performance of dairy cattle nexus with feed availability in selected peri-urban areas of Ethiopia","authors":"Z. Wondatir, Y. Mekasha, B. Wouters","doi":"10.5897/JCAB11.037","DOIUrl":"https://doi.org/10.5897/JCAB11.037","url":null,"abstract":"The study was conducted to assess the performance of dairy cattle in relation with feed availability and quality in selected peri-urban of Debre Birhan, Jimma and Sebeta areas of Ethiopia. Structured questionnaire, secondary data sources, field observations and laboratory analysis were employed to generate data. A total of 60 farmers (Debre Birhan=20, Jimma=20 and Sebeta=20) were randomly selected for the study. The overall estimated mean lactation length of cows was 296.5±8.7 days and was not different (P>0.05) among sites. The overall estimated mean age of heifers at first service was 27.5±1.0 months and age at first calving was 36.8±1.0 months and differed (P<0.001) considerably among the study sites. The result of the study indicated that grass hay was the main basal diet in all study areas. Laboratory analysis of major feed resources indicated that hay had Crude Protein (CP) content of 6.1% and crop residues varied from 3.1 to 6.7%. In addition, crop residues had lower digestibility (48%), its energy value ranged from 6.5 to 7.9 MJ/kg dry matter (DM). Wheat bran, and molasses had Metabolizable Energy (ME) content of 13.2 and 12.5 MJ/kg DM, respectively. Brewery wet grains had lower CP (27%) than cottonseed cake (42%) and enough seedcake (35%). Annual feed balance estimation revealed that the total estimated available feed supply met 83% of the maintenance DM requirement of livestock per farm per year while, the total estimated CP and ME were in accordance with the livestock requirement merely for maintenance. Therefore, from the current study it was concluded that the quality of available basal roughage feeds is generally low and strategic supplementation of protein and energy rich feeds should be required. Furthermore, optional feeds like brewery wet grains and other non-conventional feed resources should be further considered.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85941486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Elysée Nchoutpouen, Guy Benoicirc, T. L. Folefack, A. Fomena
{"title":"Structure and population dynamics of myxobolus infections in wild and cultured Oreochromis niloticus Linnaeus, 1758 in the Noun division (West-Cameroon)","authors":"Elysée Nchoutpouen, Guy Benoicirc, T. L. Folefack, A. Fomena","doi":"10.5897/JCAB.9000138","DOIUrl":"https://doi.org/10.5897/JCAB.9000138","url":null,"abstract":"Myxosporidian parasites of Oreochromis niloticus Linnaeus, 1758 from the Noun River at Kouoptamo and the Foumban fish ponds in west Cameroon, were investigated from May 2008 to June 2009. Out of 537 Tilapia (267 cultivated and 270 wild) examined, 64.8% (n=173) specimens from the fish farming and 61.1% (n=165) from the Noun River harbored Myxosporean parasites. A total of ten parasite species were found. Myxobolus kainjiae, Myxobolus sarigi were scarce in both study sites; Myxobolus Tilapiae, Myxobolus equatorialis scarce in Foumban and Kouoptamo, respectively. M. agolus, M. brachysporus, M. camerounensis, M. equatorialis, Myxobolus Heterosporus, Myxobolus israelensiswere secondary in the two sites. M. Tilapiae, M. equatorialis appeared secondary in the Noun River and the fish ponds respectively. Myxosporean spores were most encountered in the kidney (61.3 and 49.0%, respectively in cultured and wild fish) and the spleen (50.5% in Foumban and 47.5% in Kouoptamo) but no host sex preference was found. In the Foumban fish farm site, high significant infection rate was observed for M. tilapiae, M. camerounensis and M. israelensis during the rainy season, while in the Noun River, no significant seasonal effect was found. Older hosts were significantly most infected at the fish ponds while youngs Tilapia were most commonly infected in the River. \u0000 \u0000 \u0000 \u0000 Key words: Myxosporean, Oreochromis niloticus, prevalence, fish-farm, Noun River, Cameroon.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"27 1","pages":"254-264"},"PeriodicalIF":0.0,"publicationDate":"2011-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78405365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Somatic cell count, total bacterial count and acidity properties of milk in Khartoum State, Sudan","authors":"Adil M. A. Salman, H. A. Elnasri","doi":"10.5897/JCAB.9000136","DOIUrl":"https://doi.org/10.5897/JCAB.9000136","url":null,"abstract":"This work was conducted in Khartoum State to study the limits of the somatic cell count (SCC), the total bacteria count (TBC) and the acidity of the raw cow milk produced in the three geographical areas of the Khartoum State. A total of 644 stratified random raw milk samples were collected during summer and winter. The different counts and acidity were evaluated in the farm milk and compared to that sold in the market. Total bacterial count was carried out using the pour plate count. The bacterial count of equal or less than 9 × 104 cfu/ml in the state was 23.9% with a higher percentage in winter (35.4%) compared to 19.4% in summer. The majority of the samples (55.3%) had a count of less than or equal 9 × 105 cfu/ml. The percentage in winter was (71.9%) while in the summer it was (48.1%). Regarding the SCC, it was done using new man stain. The percentage of samples of less than 5 × 105 were (27%) in the state. The percentage was higher in winter (43.3%) than summer (20.8%).The majority of the samples (83.4%) were equal to or less than 7.5 × 105 (93.8%) were in winter and (81.5%) in summer. Regarding acidity which was carried out using the titration method, the percentage of samples of 0.2 titratable acidity were 64.3% in the state. The percentage in winter was 73.6% while in summer it was 60.7%. Statistically significant correlations at 0.01 levels between SCC, acidity and TBC were determined. The differences between the counts of SSC and TBC in winter and summer were statistically significant while the difference between the regions was insignificant. \u0000 \u0000 Key words: Sudan, milk hygiene, somatic cell count (SCC), total bacteria count (TBC), acidity.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"12 1","pages":"223-230"},"PeriodicalIF":0.0,"publicationDate":"2011-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83433383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The prevalence of SEN virus infection in blood donors and chronic hepatitis B and C patients in Chaharmahal Va Bakhtiari province","authors":"P. G. Dehkordi, A. Doosti","doi":"10.5897/JCAB.9000157","DOIUrl":"https://doi.org/10.5897/JCAB.9000157","url":null,"abstract":"SEN virus (SEN-V) is a blood-borne, circular, nonenveloped and single-stranded DNA virus. Phylogenetic analysis demonstrated 9 different genotypes for this virus. SEN-V could be related to post-transfusion hepatitis and infections with this virus in blood donors and hepatitis patients differ markedly by geographic region. The purpose of present study was to determine the prevalence of H and D genotypes of SEN-V (SENV-H and SENV-D) infection in blood donors and patients with chronic hepatitis B virus (HBV) and hepatitis C virus (HCV) for the first time in Chaharmahal Va Bakhtiari province located in southwest Iran. SEN-V DNA was analyzed in 240 serum samples of the patients with chronic HBV and HCV (172 HBV and 68 HCV) and 60 non-professional blood donors from the blood transfusion organization, hospital and clinical and pathological laboratories in Chaharmahal Va Bakhtiari province. SEN-V DNA was amplified by specific primers for SENV-H and SENV-D genotypes using polymerase chain reaction (PCR) method after extraction of DNA from sera and PCR products were visualized in a 1% agarose gel electrophoresis. SENV-H genotype was found to be positive in 54/172 (31.39%), 23/68 (33.82%), and 8/60 (13.33%) and SENV-D genotype was detected in 48/172 (27.91%), 27/68 (39.7%), and 6/60 (10%) of patients with chronic HBV, HCV and healthy blood donors, respectively. These results showed that high prevalence of SEN-V infection in patients with chronic HBV and HCV compared healthy blood donors in Chaharmahal Va Bakhtiari province using T test statistical analysis (P<0.05). According to these findings examination of serum samples for control and prevention of SEN-V infection in hepatitis patients and healthy blood donors seems to be necessary .","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"27 1","pages":"182-186"},"PeriodicalIF":0.0,"publicationDate":"2011-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73522662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}