Journal of Cell and Animal Biology最新文献

Histomorphology and sperm profile of animal models administered with aqueous seed extract of Parkia biglobosa 给药后动物模型的组织形态学和精子特征

Journal of Cell and Animal Biology Pub Date : 2022-05-31 DOI: 10.5897/jcab2022.0471

K. Obeten, U. Isaac, V. Archibong, Peter Adie Oshie, Ekom Etukudo, I. O. Onanuga, J. Ochieng, Ann Monima Lemuel

{"title":"Histomorphology and sperm profile of animal models administered with aqueous seed extract of Parkia biglobosa","authors":"K. Obeten, U. Isaac, V. Archibong, Peter Adie Oshie, Ekom Etukudo, I. O. Onanuga, J. Ochieng, Ann Monima Lemuel","doi":"10.5897/jcab2022.0471","DOIUrl":"https://doi.org/10.5897/jcab2022.0471","url":null,"abstract":"This study evaluated the effects of aqueous seed extract of Parkia biglobosa on the micro-anatomy of the testis and sperm profile of adult Wistar rats. Twenty-one male Wistar rats weighing between 96 and 120 g were assigned into three groups A, B, and C (n=7). Group A was the control and received normal saline, while the animals in groups B and C were treated with 300 and 500 mg/kg body weight (Bwt) aqueous seed extract of P. biglobosa orally and daily for 30 days. The animals were sacrificed via cervical dislocation at the end of the administration, and the testes were extracted for micro anatomical and histochemical studies. The Makler counting chamber was used for semen analysis while the Hematoxylin and Eosin (H and E) stain was used to assess the microstructure of the testicular tissues. The result from semen analysis showed a significant increase (p<0.05) in sperm motility, vitality, and morphology in the group treated with 300 mg/kg Bwt when compared with the control. The group treated with 500 mg/kg Bwt, showed a significant decrease in sperm motility, vitality, and morphology when compared with the control. H and E studies revealed the increased thickness of the germ cell layers of the seminiferous tubules (ST), with an increased number of spermatozoa in the lumen of ST in the group treated with 300 mg/kg bwt compared to the control group. This is a biomarker for improved spermatogenic activity in testicular tissues. Ingesting of P. biglobosa in the conditions used in this study seems to be safe and improves sperm parameters at a low dose (300 mg/kg Bwt).","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"93 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85697670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

GFSKLYFamide-like neuropeptide is expressed in the female gonad of the sea cucumber, Holothuria scabra gfsklyfamilide样神经肽在海参(Holothuria scabra)雌性性腺中表达

Journal of Cell and Animal Biology Pub Date : 2020-02-29 DOI: 10.5897/JCAB2017.0454

A. Abayomi, O. A. Nathaniel

{"title":"GFSKLYFamide-like neuropeptide is expressed in the female gonad of the sea cucumber, Holothuria scabra","authors":"A. Abayomi, O. A. Nathaniel","doi":"10.5897/JCAB2017.0454","DOIUrl":"https://doi.org/10.5897/JCAB2017.0454","url":null,"abstract":"Neuropeptides are mediators of neuronal signalling, controlling a wide range of physiological processes that cut across many organisms. GFSKLYFamide is a neuropeptide that belongs to the Echinoderm SALMFamide group of peptides. This study was aimed at determining the expression of GFSKLYFamide-like neuropeptide in the female gonad of the sea cucumber Holothuria scabra. Ten female H. scabra weighing between 62 and 175 g were collected at different times between May, 2009 and April, 2010 from the Andaman Sea at Koh Jum Island in Krabi province of Thailand for the study. Using GFSKLYFamide polyclonal antibody and Alexa 488-conjugated goat anti-rabbit IgG as primary and secondary antibodies, respectively, indirect immunofluorescence method and confocal microscope was used to localise GFSKLYFamide-like immunoreactivity in the female gonad of H. scabra. The results show GFSKLYFamide-like immunoreactivity being expressed in the sub-epithelial fibres of the coelomic epithelium. This is the first report providing evidence of GFSKLYFamide neuropeptide existence in the female gonad of H. scabra. It was suggested that further studies be carried out to investigate if there is similar existence of GFSKLYFamide neuropeptide in male gonad of H. scabra and also to know the structure or activity of the peptide(s) producing GFSKLYFamide-like immunoreactivity in H. scabra gonads. \u0000 \u0000 \u0000 \u0000 Key words: GFSKLYFamide, SALMFamides, immunoreactivity, sea cucumber, echinoderm, gonad.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"3 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2020-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90081383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic variations of indigenous sheep breed ecotypes of Ethiopia: A review 埃塞俄比亚地方羊品种生态型的表型变异研究进展

Journal of Cell and Animal Biology Pub Date : 2020-02-29 DOI: 10.5897/JCAB2019.0467

Weldeyesus Gebreyowhens Berhe

{"title":"Phenotypic variations of indigenous sheep breed ecotypes of Ethiopia: A review","authors":"Weldeyesus Gebreyowhens Berhe","doi":"10.5897/JCAB2019.0467","DOIUrl":"https://doi.org/10.5897/JCAB2019.0467","url":null,"abstract":"Updated result of the phenotypic characterization and description of the indigenous sheep population was reviewed. The reviewed paper used reporting and tabulating published descriptive phenotypic information on Ethiopian breeds with the aim of dissemination of relevant information for the beneficiaries. The original sheep breed types in Ethiopia that migrated from South West Asia to Africa and then to Ethiopia were the thin-tailed, fat-tailed and fat-rump. Through time, they evolved into 14 traditionally recognized sheep breeds which are identified and described through phenotypic characterization of the representative sample flocks. The breeds adapted to different agro ecological zones varied in topographical features and humidity. The overall reviews of the adult average live body weight variation of the local sheep population of Ethiopia are classified into 20 to 25 kg (Elle, Abergelle and Menz); 26 to 30 kg (Afar, Tikur, Highland sheep, Washera and Black Head Somali [BHS]); 31 to 35 kg (Bonga, Begait, Horro, Arusi-Bale and Farta) and 36 to 40 kg (Gumuz). The large sheep breeds of Ethiopia are Begait, Washera, Gumuz, Horro and Bonga. There is a variation on phenotypic performance among the sheep breeds of the Ethiopia in terms of body shape and size, ear profile and size, head profile, tail size and shape, coat color and on the adaptive behaviors. Molecular characterization should be done for comprehensive genetic evaluation and classification of the indigenous sheep population at national level. \u0000 \u0000 \u0000 \u0000 Key words: Sheep breed, phenotypic variation, sheep classification, tail type and tail shape.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"32 1","pages":"6-20"},"PeriodicalIF":0.0,"publicationDate":"2020-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76089125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Review on breeding objectives and practices of dairy cattle production in Ethiopia 埃塞俄比亚奶牛养殖目标与实践综述

Journal of Cell and Animal Biology Pub Date : 2019-01-31 DOI: 10.5897/JCAB2018.0457

A. Alilo

引用次数: 9

Effects of Kelulut honey from Trigona sp. on zebrafish (Danio rerio) embryo that mimics human embryonic development Trigona . Kelulut蜂蜜对模拟人类胚胎发育的斑马鱼胚胎的影响

Journal of Cell and Animal Biology Pub Date : 2018-08-31 DOI: 10.5897/jcab2018.0458

Noor Hidayat Adenan Mohd, S. Y. Latifah, Christianus Annie, Fariesha Md Hashim Nur, Rahim Harun Abdul, Hafizati Abdul Halim Nur, Zulmadi Sani Mohd, Abdul Rahim Khairuddin

引用次数: 3

Camel milk improvement of antioxidant and lipid profile in hypercholesterolemic rabbits 骆驼奶对高胆固醇血症家兔抗氧化及血脂的改善作用

Journal of Cell and Animal Biology Pub Date : 2018-03-31 DOI: 10.5897/JCAB2017.0455

Magbola S. H. Elzhrani

{"title":"Camel milk improvement of antioxidant and lipid profile in hypercholesterolemic rabbits","authors":"Magbola S. H. Elzhrani","doi":"10.5897/JCAB2017.0455","DOIUrl":"https://doi.org/10.5897/JCAB2017.0455","url":null,"abstract":"Dietary intervention can improve lipid profile and therefore impede the progress of atherosclerosis. The primary aim of this study is to assess the possible role of camel milk in enhancing the antioxidant capacity in rats fed a high fat diet. Thirty rabbits were individually divided into three groups, the control, HFD and HFD with camel milk. The control group fed on 100 g/day of NOR diet rabbit chow (USA) for three months; whereas the HFD group will be fed rabbit diet supplemented with 1.0% cholesterol plus 1.0% olive oil for same period as the control group. Hyperlipidemia significantly reduced the degree of lipid peroxidation (increase in MDA), and a decrease in individual antioxidant. Owing to its high antioxidant composition, we hypothesized that camel milk could be effective in laboratory animal model subjected to high fat diet. In this regard, camel milk showed a hypolipidemic effects in rats. The exact mechanism behind these effects may be well-related to the antioxidant status of camel milk. The high fat diet supplemented rabbits showed elevated concentrations of TC (p < 0.001), TG (p < 0.001) and lower HDL-C (p < 0.001), compared to the control group. Dietary camel milk counteracted HFD-induced hyperlipidemia. The application of an efficient nutraceuticals reducing the risk of hyperlipidemia attracts the attention of researcher in recent years. Thus, the exact cause of these effects, in particular genomic studies on milk fat should be further investigated. \u0000 \u0000 \u0000 \u0000 Key words: Hypolipidemic, antioxidant, atherosclerosis.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"116 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2018-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84638887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Specific sperm abnormalities observed in rams (Ovis aries) following cypermethrin treatment 氯氰菊酯治疗后公羊的精子异常

Journal of Cell and Animal Biology Pub Date : 2017-04-30 DOI: 10.5897/JCAB2017.0450

U. Azubuike, O. David, Rekwot Peter Ibrahim, R. J. Sankey

{"title":"Specific sperm abnormalities observed in rams (Ovis aries) following cypermethrin treatment","authors":"U. Azubuike, O. David, Rekwot Peter Ibrahim, R. J. Sankey","doi":"10.5897/JCAB2017.0450","DOIUrl":"https://doi.org/10.5897/JCAB2017.0450","url":null,"abstract":"This work was designed to make further observations on the specific types of sperm abnormalities present in Cypermethrin treated rams and contribute information towards understanding how Cypermethrin affects male’s reproduction. Sixteen sexually-mature, healthy Yankasa rams aged 18 to 30 months and weighing between 21.5 to 46.5 kg were used. The 16 rams were divided equally into two groups (A and B). Group A served as the treatment group, while B served as the control. The rams in Group A were given Cypermethrin (3%) at the dose rate of 3 mg/kg (0.1 ml/kg) body weight, topically as pour-on. The control rams were given distilled water at the same dose rate and route. These treatments were repeated every two weeks for a period of 12 weeks. Semen was collected on weekly basis from each ram during the experimental period by means of a hand held electro-ejaculator (EE). The morphological sperm abnormalities/defects were determined by examining semen smears stained with eosin-nigrosin on a glass slide. Results showed that sperm abnormalities recorded were detached head, mid piece droplet, free tail, coiled tail and bent tail. There was no significant difference in the percentage of these abnormalities recorded between the two groups (p>0.05) from week 1 to 12. It is concluded that Cypermethrin did not induce any specific sperm abnormalities in rams treated with 3 mg/kg body weight as pour-on. Sperm death in Cypermethrin label dose treated rams may be mediated through oxidative stress. It was recommended that more studies be directed towards stress biomarkers in Cypermethrin treated rams. \u0000 \u0000 Key words: Sperm, abnormalities, cypermethrin, rams, oxidative stress.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"5 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2017-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78009084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sheep and goats pasteurellosis: Isolation, identification, biochemical characterization and prevalence determination in Fogera Woreda, Ethiopia 绵羊和山羊巴氏杆菌病:埃塞俄比亚Fogera wooreda的分离、鉴定、生化特性和流行率测定

Journal of Cell and Animal Biology Pub Date : 2016-12-31 DOI: 10.5897/JCAB2016.0449

T. Alemneh, Annania Tewodros

{"title":"Sheep and goats pasteurellosis: Isolation, identification, biochemical characterization and prevalence determination in Fogera Woreda, Ethiopia","authors":"T. Alemneh, Annania Tewodros","doi":"10.5897/JCAB2016.0449","DOIUrl":"https://doi.org/10.5897/JCAB2016.0449","url":null,"abstract":"A cross sectional study was carried out on pasteurellosis of small ruminants in Fogera Woreda, Ethiopia. The objectives of this research were isolation and characterization of the bacterial species by culture and biochemical testes, and determining the prevalence in apparently pneumonic small ruminants. Out of the total 988 samples examined, 322 were detected positive for pasteurella with an overall infection rate of 32.6%. Of which, 180 (55.9%) were from nasal swabs and 142 (44.1%) were from blood samples. Accordingly, 79.5% of the isolates were Mannheimia haemolytica and 20.5% were Pasteurella multocida. Significantly (p<0.05) higher prevalence was detected in sheep (37.1%), females (36.42%), young (52.97%), and extensively managed ruminants (38.15%) than those in goats (21.9%), males (25.29%), adults (21.26%) and semi-intensive production systems (17.18%), respectively. Similarly, the frequency of infection was significantly (p<0.05) higher in winter (48.6%) and spring (32.85%) as compared to autumn (23.79%) and summer (19.67%). In conclusion, this finding showed that the disease is highly prevalent in the study area. Thus, an integrated application of vaccination and overall management measures should be implemented to prevent and control the disease in animals. \u0000 \u0000 Key words: Pasteurellosis, Isolation, Mannheimia haemolytica, Pasteurella multocida, Sheep and Goats, Identification, Fogera, Ethiopia.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"77 1","pages":"22-29"},"PeriodicalIF":0.0,"publicationDate":"2016-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90441928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

The occurrence of Cysticercus bovis at Gondar ELFORA Abattoir, Northwest of Ethiopia 埃塞俄比亚西北部Gondar ELFORA屠宰场牛囊虫的发生

Journal of Cell and Animal Biology Pub Date : 2016-09-30 DOI: 10.5897/JCAB2016.0448

Ezeddin Adem, T. Alemneh

{"title":"The occurrence of Cysticercus bovis at Gondar ELFORA Abattoir, Northwest of Ethiopia","authors":"Ezeddin Adem, T. Alemneh","doi":"10.5897/JCAB2016.0448","DOIUrl":"https://doi.org/10.5897/JCAB2016.0448","url":null,"abstract":"A cross-sectional survey was undertaken in the abattoir to study the prevalence of Cysticercus bovis in cattle originated from different localities and to determine the cyst prevalence as well as distribution in different organs within infected animals in Gondar ELFORA abattoir. Out of the total 450 cattle slaughtered and examined at Gondar ELFORA abattoir, 9 animals (2.0%) were identified positive for C. bovis infection. Predilection sites for C. bovis were observed and their relative infestation rates were recorded. As a result of this study, predominantly cysts were found on shoulder muscle (55.56%) followed by masseter muscle (33.33%) and tongue (11.11%). The prevalence and occurrence of C. bovis was also studied based on the geographical locations of slaughtered cattle. Accordingly, cattle from low lands and high lands had showed a prevalence of 6.45 and 0.307%, respectively. In conclusion, C. bovis is prevalent and is one of the major parasitic diseases that causes huge carcass condemnation of slaughtered animals and poses serious financial lose in the socio-economy of the study area. Therefore, public health awareness should be created on improving personal and environmental hygiene for breaking the life cycle of the disease. \u0000 \u0000 Key words: Cysticercus bovis, Gondar ELFORA, prevalence, Ethiopia.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"98 1","pages":"16-21"},"PeriodicalIF":0.0,"publicationDate":"2016-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77560410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Characterization and anti-tumor effect of shark liver extraction 鲨鱼肝提取物的特性及抗肿瘤作用

Journal of Cell and Animal Biology Pub Date : 2016-05-31 DOI: 10.5897/JCAB2016.0444

Y. Xi, Yunwang Zhao, C. Ren

{"title":"Characterization and anti-tumor effect of shark liver extraction","authors":"Y. Xi, Yunwang Zhao, C. Ren","doi":"10.5897/JCAB2016.0444","DOIUrl":"https://doi.org/10.5897/JCAB2016.0444","url":null,"abstract":"Shark liver oil (SLO) has been widely used as a food supplement to boost human immunity. The extraction method of shark liver oil is immature. In this study, four different extraction methods of shark liver oil were tried, the effect of the solid-liquid ratio, quality of the enzyme, reaction pH and temperature on SLO extraction rate have been discussed. Infrared spectroscopy, gas chromatography and high performance liquid chromatography was used to detect the main composition of SLO, and U14 tumor bearing mice were used to explore anti-tumor effect of SLO. Our results show that alkaline protease extraction method was the best with an extraction rate of 30.15%, and the solid-liquid ratio, quality of the enzyme, reaction pH and temperature was 1:2, 2% of the liver quality, 9, 3.5 h, 48°C respectively. Characteristic peak of alkoxy glycerol, squalene and vitamin A was presented in the IR spectra of SLO, the proportion of alkoxy glycerol, squalene content and vitamin was 35.3, 40.4 and 7.8% determined by gas chromatography (GC) and high performance liquid chromatography (HPLC). The inhibition rate was 54.56. \u0000 \u0000 Key words: Shark liver oil, extraction, characterization, gas chromatography, high performance liquid chromatography (HPLC).","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"28 1","pages":"9-15"},"PeriodicalIF":0.0,"publicationDate":"2016-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90646552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2