Journal of Cell and Animal Biology最新文献_第2页

Effect of Different Levels of Satureja Hortensis Essential Oil on Performance, Carcass Characteristics, Acidity and Intestinal Microflora Population in Broilers 不同水平马齿苋精油对肉鸡生产性能、胴体特性、酸度和肠道菌群的影响

Journal of Cell and Animal Biology Pub Date : 2016-01-31 DOI: 10.5897/JCAB2015.0440

M. Yeganeparast, M. Khalili, J. Salari

{"title":"Effect of Different Levels of Satureja Hortensis Essential Oil on Performance, Carcass Characteristics, Acidity and Intestinal Microflora Population in Broilers","authors":"M. Yeganeparast, M. Khalili, J. Salari","doi":"10.5897/JCAB2015.0440","DOIUrl":"https://doi.org/10.5897/JCAB2015.0440","url":null,"abstract":"This experiment was carried out to study the effects of dietary supplementation of Satureja hortensis essential oil on growth, carcass parts, pH and luminal microbe in broiler chicken. A total of 400 one week old ROS 308 male chicks were used in a completely randomize design model with 5 treatments and 4 replicates, each composing of 20 chicks. Duration of experiment was 35 days until the age of 42 days. Treatments were a basal diet supplemented with 50, 100 and 150 mg/kg of S. hortensis essential oil with a negative (no supplementation) and a positive control (containing 300 g/1000 kg) of Orego-Stim (a commercial compound). Feed intake and body weight gain were measured weekly and feed conversion ratio was calculated accordingly. Chicks were reared on litter and were fed ad libitum with antibiotic and coccidiostat free diet. At 35th day, litter samples was collected and sent to laboratory for determining its moisture and bacterial total count. At the 42nd day, two chicks per cage were slaughtered for determining carcass parts ratio, ileum digesta pH and bacterial count by dilution method. Results showed that S. hortensis essential oil and Orego-Stim compound had no effects on broiler growth. Liver relative weight increased by 50 and 150 ppm S. hortensis essential oil as compared to the control group. No change of pH was observed due to treatments. Chicks that received 150 ppm S. hortensis essential oil showed higher litter bacterial count as compared to the control and other treatments. \u0000 \u0000 \u0000 \u0000 Key words: Broiler chicken, Satureja hortensis essential oil, growth performance, bacterial count.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"519 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2016-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77198231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Postnatal development of the vagina in West African dwarf goat (Capra hircus) 西非矮山羊(Capra hircus)阴道的产后发育

Journal of Cell and Animal Biology Pub Date : 2015-09-30 DOI: 10.5897/JCAB2015.0439

C. Abiaezute, I. Nwaogu

{"title":"Postnatal development of the vagina in West African dwarf goat (Capra hircus)","authors":"C. Abiaezute, I. Nwaogu","doi":"10.5897/JCAB2015.0439","DOIUrl":"https://doi.org/10.5897/JCAB2015.0439","url":null,"abstract":"The postnatal developmental pattern of the vagina of WAD goat from birth to week 28 was investigated by gross and light microscopy. Forty five (45) female WAD goat kids were used in this study and were divided into nine groups of five goat kids per group. Each goat was euthanized, the vagina dissected out and studied, the length and weight measured. Data obtained were analysed by analysis of variance and DuncanE¼s new multiple range test with values of p< 0.05 considered significant. The tissues were fixed in Bouin’s fluid and routinely processed for histology. At birth, the length and weight of the vagina of the female WAD goat were 1.85 ± 0.10 cm and 0.10 ± 0.01 g, respectively. These increased significantly (p< 0.05) to 5.07 ± 0.39 cm and 4.04 ± 0.33 g at 28 weeks of age, respectively. The longitudinal folds and hymen were ill defined at birth but became increasingly prominent as the goats aged with the longitudinal folds extending into the vulva. Histologically, the epithelium in all age group was a stratified squamous type. At birth, the lamina priopria-submucosa formed the cores of the longitudinal folds composed of loose connective tissue with numerous blood vessels. The tunica muscularis was ill defined. The tunica serosa/adventitia was a loose connective tissue containing blood vessels. The vagina showed increased development of its tissues with age. The histology of the vaginal tissues at week 16 was similar to older WAD goat. In all age groups, isolated lymphoid nodules were observed in the lamina priopria-submucosa. The result suggests that the WAD goat probably attained puberty earlier than previously reported. \u0000 \u0000 \u0000 \u0000 Key words: West Africa dwarf goat, vagina, Longitudinal folds, puberty, tunica muscularis.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"29 1","pages":"31-37"},"PeriodicalIF":0.0,"publicationDate":"2015-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80489504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Sperm characteristics and haemogram of male albino rats (wistar strain) treated with saponin extract from vernonia amygdalina del. asteraeceae 110192 苦杏仁皂苷提取物对雄性白化大鼠(wistar品系)精子特性和血象的影响。asteraeceae 110192

Journal of Cell and Animal Biology Pub Date : 2015-07-30 DOI: 10.5897/JCAB2015.0432

M. Oyeyemi, K. Soetan, O. Akinpelu

{"title":"Sperm characteristics and haemogram of male albino rats (wistar strain) treated with saponin extract from vernonia amygdalina del. asteraeceae 110192","authors":"M. Oyeyemi, K. Soetan, O. Akinpelu","doi":"10.5897/JCAB2015.0432","DOIUrl":"https://doi.org/10.5897/JCAB2015.0432","url":null,"abstract":"The reproductive effects of saponin extract from Vernonia amygdalina Del. Asteraeceae 110192 leaves on 14 adult male Wistar rats was studied. The rats were divided into four groups (A, B, C and D) treated with graded doses (100, 200, 400 and 0 mg/kg respectively) of saponin extract of V. amygdalina over a period of 14 days. After 14 days oral administration of the saponin extract, the rats were sacrificed and their testicles removed through scrotal incision. Blood samples were collected periocularly into ethylene diamenetetraacetic acid (EDTA) sample bottles to prevent blood clotting. The result shows an enhancing effect at a higher dose with respect to sperm cell motility and concentration. However, the number of morphologically abnormal sperm cells was within the normal range of 10%. The packed cell volume (PCV) was slightly reduced in group C that had the highest dose (42.67±0.68%) of the saponin than for group A that had the lowest dose (47.0 ±0.24%) and (47.33 ± 0.47%) for the control group. For the morphological characteristics, there were dose dependent decrease in rudimentary tail, bent tail, curved mid-piece and bent mid-piece. In conclusion, the saponin component of V. amygdalina did not produce adverse effects on the reproductive potentials of the rats and can therefore be used to boost reproduction in male wistar rats. \u0000 \u0000 \u0000 \u0000 Key words: Sperm characteristics, saponin, Vernonia amygdalina, rats, testicles, spermatozoon, Haematology.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"11 1","pages":"26-30"},"PeriodicalIF":0.0,"publicationDate":"2015-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88659360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Body Condition Scores as Putative Diagnostic tool for African Animal Trypanosomosis among Ruminants Slaughtered at Kano Central Abattoir 身体状况评分作为卡诺中央屠宰场屠宰的非洲动物锥虫病的推定诊断工具

Journal of Cell and Animal Biology Pub Date : 2015-07-20 DOI: 10.15580/GJCAB.2015.1.022315033

A. K. Maigari, Abubakar Inuwa Malami, Anas Gide, A. Bichi, H. Sani, Aminata Musa, S. Abubakar, Samira Bello Liman, A. Sani, K. Jarmai, Z. Jega

引用次数: 2

Light and scanning electron microscopy of the small intestine of goat (Capra hircus) 山羊(Capra hircus)小肠的光镜和扫描电镜

Journal of Cell and Animal Biology Pub Date : 2015-01-30 DOI: 10.5897/JCAB2015.0425

S. Hassan, E. Moussa

{"title":"Light and scanning electron microscopy of the small intestine of goat (Capra hircus)","authors":"S. Hassan, E. Moussa","doi":"10.5897/JCAB2015.0425","DOIUrl":"https://doi.org/10.5897/JCAB2015.0425","url":null,"abstract":"Sections from duodenum, jejunum and ileum of local breeds of goat (Capra hircus) were studied histologically using light and scanning electron microscopy. The wall of goat small intestine is composed of typical layers: lamina epithelialis mucosae, lamina propria, lamina muscularis mucosae, tunica submucosa, tunica muscularis and tunica serosa. Small intestine villi were covered by a simple columnar epithelium with goblet cells and simple tubular glands, the crypts of Lieberkuhn, containing paneth cells were observed between the villi. The presence of mucus was extensive in the duodenum and the number of goblet cells was highest in the duodenum. The lamina propria consisted of loose connective tissue rich in blood and lymphatic vessels. The lamina muscularis mucosa was presented as a thin layer of circular smooth muscle fibers at the base of the crypts. The submucosa consisted of conjunctive tissue containing blood and lymphatic vessels. The tunica muscularis consisted of the typical inner circular layer and outer longitudinal layer of smooth muscle. The tunica serosa was the thin, outermost small intestine layer that consisted of a very small amount of conjunctive tissue covered by mesothelium. The submucosa was devoid of glands in all three small intestine regions. Scanning electron microscopy showed finger shaped villi in the jujenum, tongue shaped in ileum, leave like in duodenum; the villus has corrugated surface. The corrugations are deep, irregular clefts cutting into the side of the villus. The corrugations are scarce in the duodenum, few in jujenum and numerous in ileum. The surface of villi presents the goblet cell orifices. \u0000 \u0000 \u0000 \u0000 Keywords: goat, intestine","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"140 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2015-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88073896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Development of new combined method based on reading of ovarian tracheoles and the observation of follicular dilatations for determining the physiological age of Anopheles gambiae s.s. 基于卵巢气管读数和卵泡扩张观察的冈比亚按蚊生理年龄测定新方法的建立。

Journal of Cell and Animal Biology Pub Date : 2015-01-30 DOI: 10.5897/JCAB2014.0424

Rodrigue Anagonou, Fiacre R Agossa, V. Gnanguénon, B. Akinro, G. Padonou, R. Govoétchan, R. Aïkpon, A. Sovi

{"title":"Development of new combined method based on reading of ovarian tracheoles and the observation of follicular dilatations for determining the physiological age of Anopheles gambiae s.s.","authors":"Rodrigue Anagonou, Fiacre R Agossa, V. Gnanguénon, B. Akinro, G. Padonou, R. Govoétchan, R. Aïkpon, A. Sovi","doi":"10.5897/JCAB2014.0424","DOIUrl":"https://doi.org/10.5897/JCAB2014.0424","url":null,"abstract":"The conventional dilaceration of ovaries is binding at the beginning of ovary development. However, reading ovarian tracheoles was very easy in stage I-II mean. The combination of both methods can really reduce the number of age indeterminations recorded with the two methods separately. The present study aimed to identify a method capable of determining physiological age of mosquitoes regardless of their ovarian development stage. In the present study, mosquitoes were caught in houses using window traps. After identification of Anopheles gambiae s.l species, their ovaries were dissected in distilled water. An ovary was left in distilled water for tracheoles reading and the other in a physiological liquid to search for follicular dilatation after dilaceration of ovary. The other body parts of mosquitoes were used to identify the species of the A. gambiae complex by polymerase chain reaction. The ovarian tracheoles reading method was unable to determine physiological age of 25% (n=28) of 112 A. gambiae s.s. analyzed. With follicular dilations observation method, the physiological age of 16.96% (n=19) mosquitoes was not determined; but, the age indetermination rates were reduced to 0.89% by combining the two methods. The combination of ovarian tracheoles reading method and follicular dilatation observation method significantly reduced (almost null) the number of physiological age indeterminations recorded using the two methods separately. \u0000 \u0000 \u0000 \u0000 Key words: Anopheles gambiae s.s, ovary, physiological age, ovarian tracheoles, classical dilacerations, follicular dilatation.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"14 1","pages":"9-15"},"PeriodicalIF":0.0,"publicationDate":"2015-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85293424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Cd44 gene expression in mature and immature oocytes and fetus of Kermani and Baluchi sheep, and Rayeni and Tali goats Cd44基因在克尔马尼绵羊、俾路支绵羊和拉耶尼山羊、塔利山羊成熟和未成熟卵母细胞及胎儿中的表达

Journal of Cell and Animal Biology Pub Date : 2014-08-31 DOI: 10.5897/JCAB2014.0418

Mehdi Aminafshar, V. Bahrampour, Amin Baghizadeh, N. E. Kashan, M. M. Abadi

{"title":"Cd44 gene expression in mature and immature oocytes and fetus of Kermani and Baluchi sheep, and Rayeni and Tali goats","authors":"Mehdi Aminafshar, V. Bahrampour, Amin Baghizadeh, N. E. Kashan, M. M. Abadi","doi":"10.5897/JCAB2014.0418","DOIUrl":"https://doi.org/10.5897/JCAB2014.0418","url":null,"abstract":"CD44 family belongs to a large group of proteins that bind to hyaluronic acid. It has important role in oocyte maturation, fertilization and embryo development. We analyzed CD44 in oocytes and embryos of goat and different breeds of sheep. We used both Kermani and baluchi breeds of sheep, two Rayeni and Tali goats. Domestic animals kept after feeding for 3 months exude embryos and oocyte. After this, the animals were cured by keeping full progesterone for 14 days in the vagina. Gonadotropin-releasing hormone (GnRH) hormone was injected from the animals’ vagina and made ready for sampling after 1 day. After that, mature oocyte was exuded from the animals’ uterus and they were taken for slaughter. To get the embryos, male animals were kept beside them. After 6 days these animals were taken for slaughter. In another way, ovary collected from the slaughter house exuded immature oocyte. In the laboratory, immature and mature oocyte was produced. So by keeping sperm under environmental condition embryo culture is produced. The samples exude RNA using kit; after that, cDNA was produced by special protocol. The products produced by conventional PCR and Real time PCR were studied. Result shows that gene expression does not exist in immature oocyte of sheep. In Tali goat, expression of this gene was more than in Rayeni goat. \u0000 \u0000 \u0000 \u0000 Key words: CD44 gene, gene expression, real time PCR, goat, sheep.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"1 1","pages":"156-160"},"PeriodicalIF":0.0,"publicationDate":"2014-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90392842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of Aflatoxin B1 on the skeletal system of rabbit (Oryctolagus cuniculus) fetuses 黄曲霉毒素B1对兔胎儿骨骼系统的影响

Journal of Cell and Animal Biology Pub Date : 2014-08-31 DOI: 10.5897/JCAB2014.0420

S. El-Nahla, H. Imam, E. Moussa, Amr R Ghanam

{"title":"Effects of Aflatoxin B1 on the skeletal system of rabbit (Oryctolagus cuniculus) fetuses","authors":"S. El-Nahla, H. Imam, E. Moussa, Amr R Ghanam","doi":"10.5897/JCAB2014.0420","DOIUrl":"https://doi.org/10.5897/JCAB2014.0420","url":null,"abstract":"The present study was carried out to determine the effects of aflatoxinB1 (AFB1) on the skeletal system of the fetuses of balady rabbits. The female animals were divided into three groups, one control and two treated, the control group contained four dams, the intoxicated group with 0.1 mg/kg contained six dams while the intoxicated with 0.05 mg/kg contained three dams. A dose of 0.1 and 0.05 mg/kg/day AFB1 was administered by gastric intubation to pregnant rabbits on the 6th-21st day of pregnancy. The fetuses were obtained through the uterine incisionat different stages of gestation according to the group. The lengths and weights of the fetuses as well as absolute organs weights were measured, revealing the statistically significant differences between the control and intoxicated with 0.05 mg/kg group at 29th day of gestation (p<0.001) while there were non-significant decrease of control and intoxicated with 0.1 mg/kg at 22nd day of gestation. The observed gross anomalies included wrinkled skin, enlarged eye socket and microphthalmic eyes in both groups. The heart of treated group showed reduction in size with wide ventricular lumen and shallow inter ventricular groove in intoxicated group with a dose of 0.05 mg/kg AFB1. Regarding the skeletal anomalies, there were incomplete ossification in some of the skull bones, the laminae of the vertebral arches throughout the vertebral column remain cartilaginous. The sternum was incompletely ossified. Most of the appendicular skeleton bones were grossly shorter and remained in cartilaginous state. \u0000 \u0000 \u0000 \u0000 Key words: Aflatoxin B1 (AFB1), rabbits, teratogenicity, ossification.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"12 1","pages":"136-155"},"PeriodicalIF":0.0,"publicationDate":"2014-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76699558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparison study of oral disc morphology of Saudi Bufo dhufarensis and Rana ridibunda tadpoles and their oral deformities 沙特蟾蜍与沙蛙蝌蚪口盘形态及口腔畸形的比较研究

Journal of Cell and Animal Biology Pub Date : 2014-07-31 DOI: 10.5897/JCAB2013.0414

G. Bekhet

{"title":"Comparison study of oral disc morphology of Saudi Bufo dhufarensis and Rana ridibunda tadpoles and their oral deformities","authors":"G. Bekhet","doi":"10.5897/JCAB2013.0414","DOIUrl":"https://doi.org/10.5897/JCAB2013.0414","url":null,"abstract":"The microanatomy of the oral discs of the tadpoles of Bufo dhafarensis and Rana ridibunda were described. Tadpoles of stage 49 and 41, respectively were examined and analyzed using scanning electron microscope. In Rana tadpoles, the mouth was ventral and the oral disk opening had the same vertical and horizontal opening size. While in Bufo tadpoles, the mouth was anterio-ventral and the oral disc had its horizontal opening larger than the vertical one. The border of oral disc of tadpoles of B. dhufarensis was surrounded with 22 marginal papillae and 8 submarginal papillae and a dorsal gap was observed. The oral disc of Bufo had less number of marginal papillae where they were short and had broad smooth ends with no apical parts. On the other hand, the border of the Rana’s oral disc was surrounded by 50 marginal papillae and 10 submarginal where the dorsal gap was also observed. Both marginal and the submarginal papillae were long, numerous, closely spaced and ending with 3-7 apical parts in each papilla. The labial tooth row formula (LTRF) of the tadpoles of B. dhufrenensis was 2/3, while that of the tadpoles of R. ridibunda pallas was 2(1)/3(1-2). In B. dhufarensis, the labial teeth were keratinized, short ending with 4-7 terminal cusps and arranged as a single row. On the contrary, the labial teeth of R. ridibunda pallas were elongated, closely spaced and numerous. These labial teeth were noticed to be either vertically positioned or curved downwards carrying 3-4 terminal cusps in their free ends. Herein, we described Saudi Arabian Amphibian B. dhufrenensis and R. ridibunda pallas by utilizing scanning electron micrographs. In the present study, we described deformities in the oral disc of B. dhufrenensis and R. ridibunda pallas tadpoles in their natural conditions. \u0000 \u0000 Key words: Oral disc, tadpoles, Rana ridibunda, Bufo dhufarensis, labial tooth row formula, deformities.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"195 1","pages":"124-135"},"PeriodicalIF":0.0,"publicationDate":"2014-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74882776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Cleft palate reconstruction by platelet-rich-plasma and stem cell injection: Histological evidences 富血小板血浆和干细胞注射重建腭裂:组织学证据

Journal of Cell and Animal Biology Pub Date : 2014-07-31 DOI: 10.5897/JCAB2014.0415

S. Tavakolinejad, D. H. Alamdari, S. Khajehahmadi, Elnaz Khordad, A. Ebrahimzadeh-Bideskan

{"title":"Cleft palate reconstruction by platelet-rich-plasma and stem cell injection: Histological evidences","authors":"S. Tavakolinejad, D. H. Alamdari, S. Khajehahmadi, Elnaz Khordad, A. Ebrahimzadeh-Bideskan","doi":"10.5897/JCAB2014.0415","DOIUrl":"https://doi.org/10.5897/JCAB2014.0415","url":null,"abstract":"Cleft palate (CP) is a common congenital defect. It causes serious problem to cleft-affected children. The gold standard of care is autogenous bone grafting which may cause additional problems together with long and extensive medical interventions. Tissue engineering is a promising solution for a widespread range of defects and disorders. It is reasonable to utilize this novel technology for CP management. Stem cells and growth factors play essential role in tissue engineering, so we evaluate effectiveness of adipose tissue and cord blood stem cells along with platelet rich plasma (PRP) on CP reconstruction. Human umbilical cord blood mononuclear cells (hUCB-MNCs) and human adipose-derived stem cells (hADSCs) were collected and incubated with Bromodeoxyuridine (BrdU) for labeling. The same was done to osteogenically differentiated hADSCs. Palatal bone defects were surgically made in rats. Afterwards, the labeled cells were mixed either with PRP or Aminoplasmal and injected to the defect borders. Immunohistochemistry and morphometry analysis were performed 4 weeks later. Data showed a significant difference in cleft size between cell-injected and control groups while the cleft site was filled with connective tissue rather than osseous tissue. Moreover, immunohistochemistry findings proved the presence of labeled cells in the surrounding tissue. These cells were detectable both in osseous and connective tissues. This study revealed the feasibility of stem cell and PRP application according to CP reconstruction. Hence, further investigation toward tissue engineering in CP may eliminate bone harvesting and its negative consequences. \u0000 \u0000 \u0000 \u0000 Key words: Cleft palate, platelet-rich-plasma, cell therapy, stem cells, tissue engineering.","PeriodicalId":15216,"journal":{"name":"Journal of Cell and Animal Biology","volume":"56 1","pages":"114-123"},"PeriodicalIF":0.0,"publicationDate":"2014-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74965584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5