身体状况评分作为卡诺中央屠宰场屠宰的非洲动物锥虫病的推定诊断工具

A. K. Maigari, Abubakar Inuwa Malami, Anas Gide, A. Bichi, H. Sani, Aminata Musa, S. Abubakar, Samira Bello Liman, A. Sani, K. Jarmai, Z. Jega
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引用次数: 2

摘要

研究了抗体介导的山羊关节炎脑炎(CAE)病毒在山羊滑膜(GSM)细胞中的增强作用与发病时间和病变严重程度的关系。同源抗体被连续稀释以增强病毒的生长,因为这提供了最佳和亚正常浓度的抗体。细胞孵育5 d后用吉氏染色法染色。在次优抗体浓度下,早期观察到明显的细胞病变效应(CPE),表明在这些浓度下病毒增强。讨论了这些可能的流行病学意义,并与人类和动物中逆转录病毒感染的发病机制有关。关键词:CAEV, GSM细胞,抗体介导增强,细胞病变效应
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Body Condition Scores as Putative Diagnostic tool for African Animal Trypanosomosis among Ruminants Slaughtered at Kano Central Abattoir
Antibody-mediated enhancement of caprine arthritis encephalitis (CAE) virus in goat synovial membrane (GSM) cells was investigated with regards to time of onset and severity of lesions. Homologous antibody was serially diluted to enhance the growth of the virus as this provided both optimal and subnormal concentrations of the antibody. The cells were incubated for five days and later stained with Giemsa stain. Marked cytopathic effects (CPE) were observed early at sub-optimal concentrations of the antibody indicating that the virus was enhanced at these concentrations. Possible epidemiological implications of these are discussed with relevance to the pathogenesis of retrovirus infections in man and animals.   Key words: CAEV, GSM cells, antibody-mediated enhancement, cytopathic effects.
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