Effect of aqueous ozone solution on pancreatic cells

The aim of this study was to evaluate the effects of ozone solutions on the viability of pancreatic cells. The ozone was generated by an electrical discharge on high-purity oxygen molecules. Ozonation of the water was performed by bubbling ozone through sterile distilled water (O3 concentration 4 g/1000 L) and saline solution (O3 concentration 5 g/1000 L). After initial dilution of test solutions, serial dilutions were made. In total, seven were tested for both types of solutions (0.00312 mg O3/1000 L, 0.00625 mg O3/1000 L, 0.0125 mg O3/1000 L, 0.025 mg O3/1000 L, 0.05 mg O3/1000 L, 0.1 mg O3/1000 L and 0.2 mg O3/1000 L). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra-zoliumbromide (MTT) cytotoxicity test was performed in triplicate after 24 h of treatment.