Medical Parasitology and Parasitic Diseases最新文献_第2页

STUDIES ON TICKS OF THE GENUS DERMACENTOR (ACARI; IXODIDAE) TO NATURAL OCCURENCE CAUSE OF Q FEVER UNDER CONDITIONS CENTRAL CAUCASIAN REGION 革蜱属蜱类的研究依蚊科)到自然发生的q热的原因在高加索中部地区的条件

Medical Parasitology and Parasitic Diseases Pub Date : 2022-12-01 DOI: 10.33092/0025-8326mp2022.4.3-8

E. Lazarenko, N. V. Ermolova, A. Zhiltsova, L. Shaposhnikova

{"title":"STUDIES ON TICKS OF THE GENUS DERMACENTOR (ACARI; IXODIDAE) TO NATURAL OCCURENCE CAUSE OF Q FEVER UNDER CONDITIONS CENTRAL CAUCASIAN REGION","authors":"E. Lazarenko, N. V. Ermolova, A. Zhiltsova, L. Shaposhnikova","doi":"10.33092/0025-8326mp2022.4.3-8","DOIUrl":"https://doi.org/10.33092/0025-8326mp2022.4.3-8","url":null,"abstract":"The purpose of the research: to assess the natural occurrence of Coxiella burnetii in ticks of the genus Dermacentor; clarify of the relationship between the physiological age of ticks and their infection with the Q fever patogen. Materials and methods: In the period from 2017 to 2019, we examined 4908 specimens of Dermacentor marginatus (616 pools) 4208 specimens of Dermacentor reticulatus (566 pools) and 43 specimens of Dermacentor niveus (18 pools) were examined for infection with the Q fever patogen. To assess the dependence of the detection of Coxiella burnetii in ticks depending on the physiological age, we examined 3048 specimens D. reticulatus (381 pools) and 2319 specimens D. marginatus (366 pools). The study of ixodid ticks for the presence of Coxiella burnetii DNA of the causative agent of Q fever was carried out by the molecular genetic method, namely the polymerase chain reaction (PCR), in accordance with the regulatory guidelines. Results and discussion: The results of the research showed that the infection of ixodid ticks with Coxiella in the Central Ciscaucasian natural populations on D. reticulatus and D. marginatus fluctuates over the years and does not exceed one percent. When considering the dependence of the detection of Coxiella burnetii in ticks on their physiological age, statistically significant results were obtained only for D. reticulatus. There is a significant correlation between the more frequent detection of infected ticks of physiological age II compared with ticks of physiological age III and IV. For D. marginatus ticks, there was no statistically significant relationship between the detection of Q fever pathogen and physiological age. Key words: ixodid ticks, Dermacentor reticulatus, Dermacentor marginatus, Dermacentor niveus, Q fever, physiological age.","PeriodicalId":152020,"journal":{"name":"Medical Parasitology and Parasitic Diseases","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127878166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE RELEVANCE OF USING THE SOFTWARE PACKAGE R-MALDIQUANT FOR THE CONVERSION OF RAW MAA-SPECTRA OF DIROFILARIA 使用r-maldiquant软件包进行魔丝虫原始maa光谱转换的相关性

Medical Parasitology and Parasitic Diseases Pub Date : 2022-12-01 DOI: 10.33092/0025-8326mp2022.4.16-22

S. Nagorny

{"title":"THE RELEVANCE OF USING THE SOFTWARE PACKAGE R-MALDIQUANT FOR THE CONVERSION OF RAW MAA-SPECTRA OF DIROFILARIA","authors":"S. Nagorny","doi":"10.33092/0025-8326mp2022.4.16-22","DOIUrl":"https://doi.org/10.33092/0025-8326mp2022.4.16-22","url":null,"abstract":"Diagnosis of human tissue helminthiasis remains a complex problem of practical medicine. Therefore, the search for alternative direct methods of their verification is an important area of laboratory diagnostics. Proteomic research methods are one of the most modern areas of laboratory practice. Updating the database of reference protein profiles of parasitic pathogens is one of the main priorities of mass spectrometry. To convert digital data of protein extract spectra, the R-MALDIquant software package for dirofilaria differentiation (Dirofilaria immitis, Dirofilaria repens) was used. For the study, two D. repens females were taken, removed from the subcutaneous fat of two patients (one from each patient), two D. immitis females obtained from the heart after autopsy of two fallen dogs. In the study, only the head ends were taken (where the stichocytes are concentrated), without reproductive organs (where the main excretory-secretory antigens of the parasite are located). The profiles of the mass spectra of the homogenate proteins were obtained using the standard Sepsityper Kit 50 and the “Formic Acid Extraction” for Microflex LT MALDI–TOF MS (Bruker Daltonics) with flexControl software (Bruker Daltonics). Visualization was performed using Flexanalysis 3.3 software (Bruker Daltonics). Preliminary processing of mass spectra was carried out using the R software environment in the MALDIquant package. The coincidence rate in the dendrogram sections for D. immitis individuals was 96-99 %, and for D. repens – 73-100 %, which is probably due to phenotypic differences in helminths isolated from different patients. The results obtained by us of processing two types of dirofilaria using the R-MALDIquant software environment are the first step in creating a library of reference protein profiles of nematodes of the genus Dirofilaria. Key words: Dirofilaria repens, Dirofilaria immitis, MALDI-TOF MS, R package, MALDIquant. erroneous identification of the causative agent of the disease D. latum, which did not affect the results of treatment","PeriodicalId":152020,"journal":{"name":"Medical Parasitology and Parasitic Diseases","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115069761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LABORATORY INVESTIGASTIONS OF MALARIA AND DIROFILARIASES FOR EPIDEMIOLOGICAL MONITORING IN TULA REGION, RUSSIA, IN 2012-2021 2012-2021年俄罗斯图拉地区疟疾和双丝虫病流行病学监测的实验室调查

Medical Parasitology and Parasitic Diseases Pub Date : 2022-12-01 DOI: 10.33092/0025-8326mp2022.4.9-15

T.Yu. Derzhavina, T.Yu. Ignatova, A. V. Kolganova, I.A. Khokhlachkina

引用次数: 0

STUDY OF FLEA SENSITIVITY TO DRUGS BASED ON CHLORPYRIFOS AND ZETACYPERMETHRIN 基于毒死蜱和高效氯氰菊酯的跳蚤药物敏感性研究

Medical Parasitology and Parasitic Diseases Pub Date : 2022-12-01 DOI: 10.33092/0025-8326mp2022.4.57-61

Yu. M. Tokhov, L. Shaposhnikova, A. Zhiltsova

引用次数: 0

NEW DATA ABOUT MULTIRESISTANT GERMAN COCHROACHES IN RUSSIA 关于多重耐药德国cochachache在俄罗斯的新数据

Medical Parasitology and Parasitic Diseases Pub Date : 2022-12-01 DOI: 10.33092/0025-8326mp2022.4.23-30

O. Eremina, O.Yu. Olifer, T. A. Davlianidze

{"title":"NEW DATA ABOUT MULTIRESISTANT GERMAN COCHROACHES IN RUSSIA","authors":"O. Eremina, O.Yu. Olifer, T. A. Davlianidze","doi":"10.33092/0025-8326mp2022.4.23-30","DOIUrl":"https://doi.org/10.33092/0025-8326mp2022.4.23-30","url":null,"abstract":"Resistance to insecticides from various chemical groups was assessed under laboratory conditions, on several strains of German cockroach, Blattella germanica (L.), obtained from populations collected from food facilities in Moscow, Moscow Region (Krasnogorsk, Dmitrov), and Magnitogorsk.. It was established that German cockroaches of all the studied strains had the highest resistance to cypermethrin (92-> 4000×). Resistance to chlorpyrifos was also high (30-50×), only cockroaches from Magnitogorsk were tolerant to this active ingredient (5×). For the first time in Russia, extremely high cockroach resistance to fipronil (23-192×) has been established. In the studied strains of German cockroach, susceptibility or tolerance to neonicotinoids was recorded. Resistance ratios for thiamethoxam were 2.2-10× and for its active metabolite clothianidin were (1.3-9.6×). Mosaic resistance to indoxacarb (3.2-31×) and chlorfenapyr (2.5-60×) was found in different strains of German cockroach. Possible mechanisms of insect multiresistance to insecticides are discussed. Key words: German cockroach, Blattella germanica, resistance, insecticides.","PeriodicalId":152020,"journal":{"name":"Medical Parasitology and Parasitic Diseases","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123943402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SPATIAL PATTERNS OF TRICHINOSIS DISTRIBUTION IN THE SOUTH OF THE RUSSIAN FAR EAST 俄罗斯远东南部旋毛虫病分布的空间格局

Medical Parasitology and Parasitic Diseases Pub Date : 2022-12-01 DOI: 10.33092/0025-8326mp2022.4.31-37

V. Mironova, E. A. Karabanova, N. Shartova

{"title":"SPATIAL PATTERNS OF TRICHINOSIS DISTRIBUTION IN THE SOUTH OF THE RUSSIAN FAR EAST","authors":"V. Mironova, E. A. Karabanova, N. Shartova","doi":"10.33092/0025-8326mp2022.4.31-37","DOIUrl":"https://doi.org/10.33092/0025-8326mp2022.4.31-37","url":null,"abstract":"The registered cases of trichinosis among the population of four federal entities of the south of the Far East (Amur Region, Jewish Autonomous Region, Transbaikal Territory and Khabarovsk Territory) in 2000-2020 were considered. A spatial analysis, mapping and visualization was carried out on the basis of data derived from state reports of the Rospotrebnadzor on the number of cases and the frequency of their registration in the context of municipalities. A typological classification of territories has been built using the developed scoring and the identification of statistically significant “hot” and “cold” spots has been provided. It has been established that the incidence of trichinosis in humans is associated with both the consumption of meat of wild animals and domestic pigs, which, apparently, is associated with the Trichinella species prevailing in each specific area. Regions with stably recorded incidence have been identified, however, the establishment of more obvious spatial patterns of the spread of trichinosis is hindered by the insufficiency and incompleteness of published data, in particular, the lack or inaccessibility of information on the Trichinella species composition and the fragmentation of data on the results of epidemiological investigations. The tested method gives an idea of the levels of risk of infection with trichinosis for the population of the region and can be used for more in-depth studies in the future. Key words: trichinosis, spatial statistics, risk assessment, hot and cold spots.","PeriodicalId":152020,"journal":{"name":"Medical Parasitology and Parasitic Diseases","volume":"122 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122851287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

INTERACTION OF PARASITES AND BACTERIA IN THE HOST 寄主体内寄生虫与细菌的相互作用

Medical Parasitology and Parasitic Diseases Pub Date : 2022-03-01 DOI: 10.33092/0025-8326mp2022.2.40-48

E. Stepanova, A. Aleshko, D.A. Medonchak, A.V. Sysolyatina, D.A. Baykov, N. Turbabina, M. Maksimova, A. Kondrashin, L. Morozova, V. Sergiev, E. Morozov, V. Rakova

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TYPING OF VISCERAL LEISHMANIASIS FOCUSES IN THE WHO EUROPEAN REGION 内脏利什曼病的分型重点在世卫组织欧洲区域

Medical Parasitology and Parasitic Diseases Pub Date : 2022-03-01 DOI: 10.33092/0025-8326mp2022.1.49-54

E. Ponirovsky, M. Baranets, D. Kadamov

{"title":"TYPING OF VISCERAL LEISHMANIASIS FOCUSES IN THE WHO EUROPEAN REGION","authors":"E. Ponirovsky, M. Baranets, D. Kadamov","doi":"10.33092/0025-8326mp2022.1.49-54","DOIUrl":"https://doi.org/10.33092/0025-8326mp2022.1.49-54","url":null,"abstract":"Based on the analysis of various variants of the composition of the parasitic system of visceral leishmaniasis in the who European region, the typification of foci is proposed depending on the nature of the relationship of vertebrate hosts of Leishmania with humans, the number of vectors, the intensity of the epidemic manifestation and its relevance to certain landscapes. The composition of the vectors of VL foci are divided into monofactorial and polypectomy; participation as a reservoir of different mammals to natural and synanthropic, on monogotari and oligosaline. According to the intensity of the epidemic manifestation, foci are ranked into hyperendemic, mesoendemic and hypoendemic. The most favorable landscapes for the existence of the parasitic system of visceral leishmaniasis are the territories occupied by savannas, woodlands, Mediterranean dry forests and shrubs. Preventive measures in the foci of visceral leishmaniasis should be carried out differentially, depending on the belonging of foci to a certain rank. Key words: leishmaniasis, visceral, hearth, natural, synanthropic, typification, carrier, reservoir, landscape","PeriodicalId":152020,"journal":{"name":"Medical Parasitology and Parasitic Diseases","volume":"130 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122839344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE ROLE OF PROTOZOA IN TUMORIGENESIS 原生动物在肿瘤发生中的作用

Medical Parasitology and Parasitic Diseases Pub Date : 2022-03-01 DOI: 10.33092/0025-8326mp2022.1.55-59

V. Ivanov, T. Sivkova

引用次数: 0

CLINICAL AND EPIDEMIOLOGICAL ASPECTS OF HUMAN TOXOCARIASIS IN THE ASTRAKHAN REGION 阿斯特拉罕地区人类弓形虫病的临床和流行病学方面

Medical Parasitology and Parasitic Diseases Pub Date : 2022-03-01 DOI: 10.33092/0025-8326mp2022.1.31-45

T. V. Nikeshina, R. S. Arakelyan, E. I. Okunskaya, G. L. Shendo, L.H. Ishmuhambetova

{"title":"CLINICAL AND EPIDEMIOLOGICAL ASPECTS OF HUMAN TOXOCARIASIS IN THE ASTRAKHAN REGION","authors":"T. V. Nikeshina, R. S. Arakelyan, E. I. Okunskaya, G. L. Shendo, L.H. Ishmuhambetova","doi":"10.33092/0025-8326mp2022.1.31-45","DOIUrl":"https://doi.org/10.33092/0025-8326mp2022.1.31-45","url":null,"abstract":"In this work the authors analyzed clinical and epidemiological situation of toxocariasis in man in the Astrakhan region from 2012 to 2019. So, toxocariasis we have analyzed 48 epidemiological records of persons infested Toxocara. The highest number of cases in humans was in 2013 and 2017, accounting for 20.8% and 25.0%. The diagnosis of “toxocariasis” was confirmed by enzyme immunoassay (ELISA) using test systems for the detection of antibodies to antigens Toxocara class G. In most of the infected individuals, toxocarosis occurred in the form of various clinical manifestations and symptoms-60.4%: in most cases, patients complained of abdominal pain-37.9%, including pain in the epigastric region-27.6% and pain in the right hypochondrium-10.3%, general weakness-34.5% and cough-31.0%. Also, a small number of infected individuals had complaints of pain in the joints of the upper extremities-10.3%, frequent fatigue-13.8%, allergic rhinitis, nausea and a slight increase in temperature-3.4%, lack of appetite-6.9%. In rare cases, patients complained of bloating, hives, feeling suffocated at night, slight weight loss, and nail dissection – 3.4% each. Key words: toxocariasis, Astrakhan region, epidemiology","PeriodicalId":152020,"journal":{"name":"Medical Parasitology and Parasitic Diseases","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121154884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0