Journal of biomechanics最新文献_第10页

The effects of different shoe stack heights and running speeds on full-body running coordination: An uncontrolled manifold analysis 不同鞋堆高度和跑步速度对全身跑步协调性的影响：一个非控制流形分析

IF 2.4 3区医学

Journal of biomechanics Pub Date : 2025-03-04 DOI: 10.1016/j.jbiomech.2025.112615

Cagla Kettner , Bernd J Stetter , Thorsten Stein

{"title":"The effects of different shoe stack heights and running speeds on full-body running coordination: An uncontrolled manifold analysis","authors":"Cagla Kettner , Bernd J Stetter , Thorsten Stein","doi":"10.1016/j.jbiomech.2025.112615","DOIUrl":"10.1016/j.jbiomech.2025.112615","url":null,"abstract":"<div><div>Stack height is a highly discussed key design feature of running shoes but its effects are not well understood. This study analyzed how shoe stack height and running speed influence full-body running coordination and motor variability structure using an uncontrolled manifold (UCM) analysis. The joint angle variability (i.e. elementary variables) was analyzed in terms of its effects on a synergy stabilizing the center of mass (CoM, i.e. performance variable). A total of 17 healthy experienced runners participated and ran at 10 and 15 km/h on a treadmill with three running shoes differing in stack height (H: 50 mm, M: 35 mm, L: 27 mm). The UCM components (UCM<sub>||</sub>, UCM <sub>Ʇ</sub> & UCM<sub>r</sub><sub>atio</sub>) were compared with statistical parametric mapping rmANOVAs for different shoes and speeds. The shoes did not show significant effects for the three UCM components. With increasing speed from 10 to 15 km/h, the joint angle coordination variability affecting the CoM (UCM<sub>Ʇ</sub>) increased and UCM<sub>r</sub><sub>atio</sub> decreased independent of the shoe condition. This indicated that stack height did not influence the motor variability structure. However, independent of the shoes, the variability affecting CoM increased which led to a weakened synergy stabilizing CoM (UCM<sub>r</sub><sub>atio</sub>). It can be suggested that the variations in the tested running speeds had a greater impact on the running coordination than those of the tested shoes within the UCM framework.</div></div>","PeriodicalId":15168,"journal":{"name":"Journal of biomechanics","volume":"183 ","pages":"Article 112615"},"PeriodicalIF":2.4,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of arm swings on lower limb kinetics during single-leg forward, vertical, and backward hopping 在单腿向前、垂直和向后跳跃中，手臂摆动对下肢动力学的影响

IF 2.4 3区医学

Journal of biomechanics Pub Date : 2025-03-02 DOI: 10.1016/j.jbiomech.2025.112605

Yu Song, Thanh Nguyen, Yu Gu, Wanyan Su, Nawfal Malik

{"title":"The effect of arm swings on lower limb kinetics during single-leg forward, vertical, and backward hopping","authors":"Yu Song, Thanh Nguyen, Yu Gu, Wanyan Su, Nawfal Malik","doi":"10.1016/j.jbiomech.2025.112605","DOIUrl":"10.1016/j.jbiomech.2025.112605","url":null,"abstract":"<div><div>Single-leg forward hopping was commonly used to evaluate knee function and quadricep strength deficits following anterior cruciate ligament (ACL) injuries. Achieving similar hopping performance between legs was insufficient to support symmetrical knee function and quadricep strength in patients following ACL injuries, likely due to movement compensation. This study aimed to quantify the effects of arm swings on lower limb kinetics during single-leg forward, vertical, and backward hopping with a focus on knee assessment. Thirty-eight injury-free participants performed single-leg forward, vertical, and backward hopping with and without arm swings on one leg. Hopping performance, duration of the jumping phase, as well as peak power and work of hip, knee, and ankle joints were calculated while jumping. Two-by-three repeated-measures analyses of variance were applied to determine the effects of arm swings and tasks (<span><math><mrow><mi>α</mi></mrow></math></span> = 0.05). Single-leg backward hopping demonstrated the greatest knee demands by the greatest peak knee power and knee work contribution and smallest peak hip and ankle power, hip and ankle work, compared to forward and vertical hopping, regardless of arm swings. Arm swings resulted in greater hopping performance and smaller knee work for all tasks. The results indicate the possibility of using single-leg backward hopping as a clinical-friendly task to evaluate knee function and quadriceps strength following ACL injuries. Standardized instructions on controlling arms are recommended when using performances from hopping tasks to assess knee function. The findings help us better understand lower limb kinetics during single-leg hopping tasks and compensatory strategies when arm swings are involved.</div></div>","PeriodicalId":15168,"journal":{"name":"Journal of biomechanics","volume":"183 ","pages":"Article 112605"},"PeriodicalIF":2.4,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimation of inlet flow rate in simulations of left atrial flows: A proposed optimized and reference-based algorithm with application to sinus rhythm and atrial fibrillation 左房血流模拟中入口流速的估计：一种基于参考的优化算法，应用于窦性心律和心房颤动

IF 2.4 3区医学

Journal of biomechanics Pub Date : 2025-03-01 DOI: 10.1016/j.jbiomech.2025.112594

Henrik Aasen Kjeldsberg , Renate B. Schnabel , Joakim Sundnes , Kristian Valen-Sendstad

{"title":"Estimation of inlet flow rate in simulations of left atrial flows: A proposed optimized and reference-based algorithm with application to sinus rhythm and atrial fibrillation","authors":"Henrik Aasen Kjeldsberg , Renate B. Schnabel , Joakim Sundnes , Kristian Valen-Sendstad","doi":"10.1016/j.jbiomech.2025.112594","DOIUrl":"10.1016/j.jbiomech.2025.112594","url":null,"abstract":"<div><div>Medical image-based computational fluid dynamics (CFD) is a valuable tool for studying cardiovascular hemodynamics and its role in vascular pathologies. However, patient-specific flow rate measurements are rare. As a remedy, individual flow rates are typically estimated using anatomical features. In this study, we considered left atrial (LA) flow and compared three commonly used models to two proposed models based on mitral valve orifice area or atrial volume. We optimized our two models against the mean and standard deviation (SD) for heart rate (HR), cardiac output (CO), and peak velocities (E-wave, S-wave) using reference values from healthy individuals. All five models were evaluated on a cohort (<span><math><mrow><mi>N</mi><mo>=</mo><mn>39</mn></mrow></math></span>), and the total error, calculated as the sum of the mean and SD, ranged from 37% to 71% for the commonly used models, while our proposed models achieved errors of 21.7% and 16.1%. For patients with atrial fibrillation (AF), we adapted our two models by removing the A-wave and minimizing relative differences in CO, E-wave velocity, and HR compared to normal sinus rhythm. The adapted models for AF showed errors of 1.3% and 1.1% when compared to the relative changes observed between AF and sinus rhythm patients. In conclusion, flow rates are one of the most sensitive yet influential parameters in cardiovascular simulations and can be standardized to improve model robustness. While fully patient-specific flow rate models are challenging to achieve, reducing variability against clinical measurements is a practical approach towards a plausible LA flow model.</div></div>","PeriodicalId":15168,"journal":{"name":"Journal of biomechanics","volume":"183 ","pages":"Article 112594"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute effects of isometric plantarflexion exercise on Achilles tendon non-uniform displacement 等距跖屈运动对跟腱不均匀位移的急性影响

IF 2.4 3区医学

Journal of biomechanics Pub Date : 2025-03-01 DOI: 10.1016/j.jbiomech.2025.112614

Roosa Parkkola , Maria Sukanen , Ra’ad M. Khair , Karin Grävare Silbernagel , Taija Finni

{"title":"Acute effects of isometric plantarflexion exercise on Achilles tendon non-uniform displacement","authors":"Roosa Parkkola , Maria Sukanen , Ra’ad M. Khair , Karin Grävare Silbernagel , Taija Finni","doi":"10.1016/j.jbiomech.2025.112614","DOIUrl":"10.1016/j.jbiomech.2025.112614","url":null,"abstract":"<div><div>Non-uniform displacement is a well-documented phenomenon of healthy tendons that has shown to be reduced among injured and aging populations. Non-uniformity is considered a biomarker of tendon health, yet immediate response to physical exercise is unknown. This study examined acute changes in Achilles tendon (AT) non-uniform displacement in response to high strain magnitude isometric plantarflexion exercise. The reliability of the method was also examined. Fourteen healthy participants (7 men, 7 women, mean ± SD age: 26.4 ± 4.8 years) performed unilateral isometric plantarflexion exercise at 90 % of maximal voluntary isometric contractions (MVIC) with 5 sets of 4 repetitions, each lasting 3 s. The contralateral leg served as control. AT displacement was measured during ramp contractions to a constant torque level (30 % of MVIC) before the exercise, between the loading sets, and six times during 72-h recovery period. AT nonuniformity (difference between maximum and minimum displacement) was analyzed from sagittal B-mode ultrasound videos using speckle tracking. Two-way repeated measures ANOVA was used to compare the values across different timepoints. Non-uniformity did not change in response to exercise and was 2.99 ± 1.52 mm before and 3.19 ± 1.42 mm immediately after exercise. The reliability of non-uniformity between trials within a single measurement session varied from moderate to excellent (ICC: 0.680–0.920). While the isometric high strain plantarflexion exercise did not acutely alter the non-uniform displacement of the AT in young healthy adults, strenuous exercises containing knee and ankle joint angle changes should be investigated to confirm adaptability of AT non-uniform displacement.</div></div>","PeriodicalId":15168,"journal":{"name":"Journal of biomechanics","volume":"183 ","pages":"Article 112614"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validation of OpenCap on lower extremity kinematics during functional tasks 功能性任务中下肢运动学的OpenCap验证

IF 2.4 3区医学

Journal of biomechanics Pub Date : 2025-02-28 DOI: 10.1016/j.jbiomech.2025.112602

Ainsley Svetek , Kristin Morgan , Julie Burland , Neal R. Glaviano

{"title":"Validation of OpenCap on lower extremity kinematics during functional tasks","authors":"Ainsley Svetek , Kristin Morgan , Julie Burland , Neal R. Glaviano","doi":"10.1016/j.jbiomech.2025.112602","DOIUrl":"10.1016/j.jbiomech.2025.112602","url":null,"abstract":"<div><div>Marker-based motion capture is a fundamental tool in biomechanical analysis, yet comes with major constraints such as time, cost and accessibility. This study aimed to validate the use of OpenCap, a free, markerless motion capture system compared to a marker-based motion capture system to measure lower extremity kinematics during functional tasks. 20 individuals from an athletic population (18 females, 2 males) performed two gait trials (walking, running) and three functional tasks (double leg squat, countermovement jump, jump-landing). Lower extremity peak joint kinematics were collected simultaneously using Vicon and OpenCap to assess the validity of markerless motion capture. Strong agreements were observed in the frontal hip plane joint kinematics across all tasks with root mean squared errors below 6°. Moderate agreements were observed in the sagittal knee plane joint kinematics (4–10°) and there was a weak agreement in the gait trials of the sagittal hip measures (>10°). The results from the study indicate the need for further research on the use of OpenCap in clinical settings. The findings align with previous studies with similar agreements observed in the frontal hip and sagittal knee measures. Validating the use of an open-source motion capture software could provide clinicians and researchers an accessible tool for in depth biomechanical assessments.</div></div>","PeriodicalId":15168,"journal":{"name":"Journal of biomechanics","volume":"183 ","pages":"Article 112602"},"PeriodicalIF":2.4,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tradeoffs in Modeling Context Dependency in Complex Trait Genetics. 复杂性状遗传学中情境依赖建模的权衡。

IF 2.4 3区医学

Journal of biomechanics Pub Date : 2025-02-28 DOI: 10.1101/2023.06.21.545998

Eric Weine, Samuel Pattillo Smith, Rebecca Kathryn Knowlton, Arbel Harpak

{"title":"Tradeoffs in Modeling Context Dependency in Complex Trait Genetics.","authors":"Eric Weine, Samuel Pattillo Smith, Rebecca Kathryn Knowlton, Arbel Harpak","doi":"10.1101/2023.06.21.545998","DOIUrl":"10.1101/2023.06.21.545998","url":null,"abstract":"<p><p>Genetic effects on complex traits may depend on context, such as age, sex, environmental exposures or social settings. However, it is often unclear if the extent of context dependency, or Gene-by-Environment interaction (GxE), merits more involved models than the additive model typically used to analyze data from genome-wide association studies (GWAS). Here, we suggest considering the utility of GxE models in GWAS as a tradeoff between bias and variance parameters. In particular, We derive a decision rule for choosing between competing models for the estimation of allelic effects. The rule weighs the increased estimation noise when context is considered against the potential bias when context dependency is ignored. In the empirical example of GxSex in human physiology, the increased noise of context-specific estimation often outweighs the bias reduction, rendering GxE models less useful when variants are considered independently. However, we argue that for complex traits, the joint consideration of context dependency across many variants mitigates both noise and bias. As a result, polygenic GxE models can improve both estimation and trait prediction. Finally, we exemplify (using GxDiet effects on longevity in fruit flies) how analyses based on independently ascertained \"top hits\" alone can be misleading, and that considering polygenic patterns of GxE can improve interpretation.</p>","PeriodicalId":15168,"journal":{"name":"Journal of biomechanics","volume":"35 4 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10871201/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77919891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Load transfer between active and passive lumbar tissues and its implications in time-dependent EMG-assisted biomechanical modeling 主动和被动腰椎组织之间的负荷转移及其在时间依赖性肌电辅助生物力学建模中的意义

IF 2.4 3区医学

Journal of biomechanics Pub Date : 2025-02-27 DOI: 10.1016/j.jbiomech.2025.112600

Sang Hyeon Kang , Gary A. Mirka

{"title":"Load transfer between active and passive lumbar tissues and its implications in time-dependent EMG-assisted biomechanical modeling","authors":"Sang Hyeon Kang , Gary A. Mirka","doi":"10.1016/j.jbiomech.2025.112600","DOIUrl":"10.1016/j.jbiomech.2025.112600","url":null,"abstract":"<div><div>Electromyography (EMG)-assisted biomechanical models of the lumbar spine have been developed to estimate spinal loading, but these models often have limited representation of passive tissue contributions to the trunk extension moment. Recent evidence suggests that sustained near full trunk flexion can lead to increased contribution of the passive tissues to resist the external moment due to increased lumbar flexion as the extensor muscle fatigue. This leads to our hypothesis that spinal loading might be increased due to load transfers between active and passive tissues. Sixteen participants maintained a trunk flexion posture that was ten degrees less than the trunk flexion angle inducing flexion-relaxation of erector spinae muscles for 12 min with breaks every three minutes. Trunk kinematic and EMG measures were collected. A muscle fatigue-modified EMG-assisted model with passive tissue components was employed to estimate the time-dependent force and moment profiles at the L4/L5 level. Results revealed that these postures led to a time-dependent increase in the proportion of passive tissues to resist the external moment (39.9 % to 49.5 %) during each 3-minute time block, thereby resulting in the time-dependent increase in the compression and anterior-posterior shear forces of the L4/L5 disc by 181.7 N and 125.2 N, respectively (all p-value < 0.001). These results indicate that the load transfer from active to passive tissues can lead to increased compression and anterior-posterior shear forces of the L4/L5 disc at a constant external moment. This study suggests that a time-dependent approach to an EMG-assisted model with passive tissue components can provide more accurate estimates of tissue stresses.</div></div>","PeriodicalId":15168,"journal":{"name":"Journal of biomechanics","volume":"183 ","pages":"Article 112600"},"PeriodicalIF":2.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143529201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proactive modifications to walking stability under the threat of large, anterior or posterior perturbations 在大的前或后摄动的威胁下，主动修改行走稳定性

IF 2.4 3区医学

Journal of biomechanics Pub Date : 2025-02-26 DOI: 10.1016/j.jbiomech.2025.112599

James B. Tracy , Jocelyn F. Hafer , J. Hendrik Reimann , Thomas A. Buckley , Jessica L. Allen , Jeremy R. Crenshaw

{"title":"Proactive modifications to walking stability under the threat of large, anterior or posterior perturbations","authors":"James B. Tracy , Jocelyn F. Hafer , J. Hendrik Reimann , Thomas A. Buckley , Jessica L. Allen , Jeremy R. Crenshaw","doi":"10.1016/j.jbiomech.2025.112599","DOIUrl":"10.1016/j.jbiomech.2025.112599","url":null,"abstract":"<div><div>Biomechanically, falling after a walking perturbation may be influenced by: (1) the pre-perturbation state of mechanical stability (e.g., stability margins) and (2) the response to a perturbation (i.e., recovery skill). Walking stability margins must be modifiable to serve as a target for fall-prevention interventions. We investigated if neurotypical adults could proactively modulate pre-perturbation anteroposterior stability margins while walking. Eleven adults walked on a treadmill at three speeds with and without anterior and posterior perturbations. We measured stability margins anteriorly at mid-swing and posteriorly at foot strike for pre-perturbation steps. A repeated-measures factorial ANOVA evaluated main effects and interactions of walking speed (0.6, 0.8, 1.0 stats/s) and perturbation type (anterior, none, posterior). With posterior perturbation threats, the posterior stability margins were more positive at foot strike (p < 0.01) compared to trials without perturbations. With anterior perturbation threats, the anterior stability margins were not different at mid-swing compared to trials without perturbations (p > 0.05). With any perturbation threat, step lengths shortened (p < 0.01) and step rates increased (p < 0.01). Step width was not different (p > 0.11). At slow speeds with posterior perturbation threats, double-support time decreased (p = 0.04). Proactive modifications to stability margins are indeed possible in a neurotypical population. Consequently, anteroposterior stability margins may be a feasible target for fall-prevention interventions by targeting decreased step lengths or increased step rates within a given walking speed. We do not know the extent to which the observed effects have a meaningful effect on perturbation recovery.</div></div>","PeriodicalId":15168,"journal":{"name":"Journal of biomechanics","volume":"183 ","pages":"Article 112599"},"PeriodicalIF":2.4,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological analysis of the median nerve in the carpal tunnel during wrist movements, finger pinching and palm loading 手腕运动、手指捏紧和手掌加载时腕管内正中神经的形态分析

IF 2.4 3区医学

Journal of biomechanics Pub Date : 2025-02-26 DOI: 10.1016/j.jbiomech.2025.112601

Long Yin , Yuan Guo , Xushu Zhang , Haibo Ke , Fengyi Zhou , Yiting Du , Kai Zhang , Binping Ji , Yunpeng Wen

{"title":"Morphological analysis of the median nerve in the carpal tunnel during wrist movements, finger pinching and palm loading","authors":"Long Yin , Yuan Guo , Xushu Zhang , Haibo Ke , Fengyi Zhou , Yiting Du , Kai Zhang , Binping Ji , Yunpeng Wen","doi":"10.1016/j.jbiomech.2025.112601","DOIUrl":"10.1016/j.jbiomech.2025.112601","url":null,"abstract":"<div><div>The aim of this study was to analyze the morphology of the median nerve (MN) in the carpal tunnel during hand motion and palmar load in healthy participants using ultrasound. Twenty healthy participants (10 men and 10 women) were enrolled in the study. Wrist flexion angle is negatively correlated with cross-sectional area (CSA) and perimeter, and positively correlated with circularity, whereas wrist extension angle is negatively correlated with MNCSA and circularity. At 15°, 45°, and 60° wrist flexion and extension, both MNCSA and perimeter were significantly smaller than at neutral (all <em>P</em> < 0.05). MN circularity was significantly greater at 30°, 45°, and 60° wrist flexion than at neutral (all <em>P</em> < 0.05). At 30° ulnar deviation of the wrist, MNCSA and perimeter were significantly smaller than the neutral position, while circularity was significantly larger (all <em>P</em> < 0.05). At 15° of wrist radial deviation, MNCSA and perimeter were significantly smaller than at the neutral position (all <em>P</em> < 0.05). Compared to the 40 % maximum voluntary effort (MVE), thumb-ring finger pinch (10 %MVE) resulted in significantly higher MNCSA and perimeter, while circularity was noticeably smaller (all <em>P</em> < 0.05). In the neutral position, no differences were observed in MNCSA, perimeter and circularity unloaded at 100 g, 200 g, 300 g, 400 g and 500 g palmar loads (all <em>P</em> > 0.05). The results indicate that wrist positions involving flexion, extension, and deviations, as well as finger pinch, can significantly impact the morphology of the MN. This is an important step in understanding the biomechanics of MN compression within the carpal tunnel.</div></div>","PeriodicalId":15168,"journal":{"name":"Journal of biomechanics","volume":"183 ","pages":"Article 112601"},"PeriodicalIF":2.4,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic compression modulates anabolic and catabolic activity in chondrocyte seeded agarose constructs 动态压缩调节软骨细胞种子琼脂糖结构中的合成代谢和分解代谢活性

IF 2.4 3区医学

Journal of biomechanics Pub Date : 2025-02-22 DOI: 10.1016/j.jbiomech.2025.112598

Lea Zila , Roberto Tarantino , Peter Zastawny , Stephen D Waldman

{"title":"Dynamic compression modulates anabolic and catabolic activity in chondrocyte seeded agarose constructs","authors":"Lea Zila , Roberto Tarantino , Peter Zastawny , Stephen D Waldman","doi":"10.1016/j.jbiomech.2025.112598","DOIUrl":"10.1016/j.jbiomech.2025.112598","url":null,"abstract":"<div><div>Mechanical stimulation is a widely used technique in the development of tissue engineered cartilage. While various regimes can enhance tissue growth and improve construct mechanical properties, existing outcome measures predominantly assess the anabolic effect of mechanical stimuli. Catabolic responses are generally overlooked, and a critical gap remains in how mechanical loading simultaneously affects both anabolic and catabolic processes. In this study, full-thickness articular cartilage was aseptically harvested from the metacarpal-phalangeal joints of skeletally mature bovine. Isolated chondrocytes were encapsulated in agarose gels and subjected to dynamic compressive strains from 0 % to 15 % for either 20 or 60 min using a custom-built mechanical stimulation device. Anabolism was assessed by [<sup>3</sup>H]-proline and [<sup>35</sup>S]-sulfate incorporation, while catabolism was evaluated by MMP-13 enzymatic activity. Long-term effects of dynamic loading were assessed through biochemical analyses and histological evaluation. Results showed that low-to-moderate strains (2.5 % and 5 %) induced high anabolic activity relative to control with minimal catabolic response. In contrast, high strains (15 %) resulted in elevated catabolic and reduced anabolic activity relative to control. The application of mechanical stimuli over the long-term elicited comparable responses with lower compressive stains leading to improved cartilaginous extracellular matrix accumulation. This study provides valuable insights into the complex interplay between anabolic and catabolic metabolism in chondrocyte-seeded agarose constructs subjected to dynamic compression. This research underscores the necessity of evaluating both responses to optimize the growth and properties of tissue-engineered cartilage.</div></div>","PeriodicalId":15168,"journal":{"name":"Journal of biomechanics","volume":"183 ","pages":"Article 112598"},"PeriodicalIF":2.4,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0