Journal of Bioscience and Applied Research最新文献_第6页

Detection of DNA damage by SCD and Rate of Apoptosis DNA by Gel Electrophoresis among infertile males SCD检测不育男性DNA损伤及凝胶电泳检测DNA凋亡率

Journal of Bioscience and Applied Research Pub Date : 2022-08-25 DOI: 10.21608/jbaar.2022.256197

N. Eskarous, Sobhy Hassab El-Nabi, M. A. Abd El Salam, K. Geba, S. G. GamalEl Din

{"title":"Detection of DNA damage by SCD and Rate of Apoptosis DNA by Gel Electrophoresis among infertile males","authors":"N. Eskarous, Sobhy Hassab El-Nabi, M. A. Abd El Salam, K. Geba, S. G. GamalEl Din","doi":"10.21608/jbaar.2022.256197","DOIUrl":"https://doi.org/10.21608/jbaar.2022.256197","url":null,"abstract":": Background: DNA damage as Fragmentation has adverse effects on fertilization and embryo development, so it is one of the main causes of a male factor for infertility. Several techniques have been mentioned to elevation this damage. In our study, we determine DNA damage in human spermatozoa by sperm chromatin dispersion (SCD) method and Apoptosis of DNA in human spermatozoa by Optical density in gel electrophoresis in male infertility. Objects and Methods: Semen samples were collected from 100 men and were analyzed by standard light microscopic according to the World Organization (5 th edition) for diagnostic fertility. Furthermore, Sperm DNA damage was determined by using Halosperm Kit, then assessment apoptosis by optical density in Gel Electrophoresis. Results : The mean value of DNA by SCD method in infertile males increased with a value of 47.95±10.96 % when compared with the control value of 21.2 ±2.64 % with (p< 0.00001). On the other hand, the mean value of DNA by measurement of Optical density in Gel Electrophoresis in infertile males decreased with a value of 120.27±18.73 when compare with the control value of 144.4±45 with (p =0.833). Conclusion: by SCD method and other methods for detection of DNA apoptosis by gel electrophoresis addition to routine semen analysis play important role in the diagnosis and management of male infertility.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89909102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial activity of Lactobacillus acidophilus against pathogenic Escherichia coli isolated from diarrhea patients 嗜酸乳杆菌对腹泻致病性大肠杆菌的抑菌活性研究

Journal of Bioscience and Applied Research Pub Date : 2022-08-22 DOI: 10.21608/jbaar.2022.255697

F. Kadhum

引用次数: 0

Genetic study of I kappa B alpha gene promoter polymorphism associated with hepatitis C virus in Egyptian patients 埃及丙型肝炎病毒患者I κ B α基因启动子多态性的遗传学研究

Journal of Bioscience and Applied Research Pub Date : 2022-08-20 DOI: 10.21608/jbaar.2022.255496

Mamoun Ghazalah, Sameer El-Masry, I. Helmy, Ehab Abd-Elkhalek

{"title":"Genetic study of I kappa B alpha gene promoter polymorphism associated with hepatitis C virus in Egyptian patients","authors":"Mamoun Ghazalah, Sameer El-Masry, I. Helmy, Ehab Abd-Elkhalek","doi":"10.21608/jbaar.2022.255496","DOIUrl":"https://doi.org/10.21608/jbaar.2022.255496","url":null,"abstract":"Background: Host genetic polymorphism is one of the major unalterable major factors for HCV infection, NF-κB proteins play multiple roles in immune response and involve in HCV infection and progression. Aim of the study: To investigate the associations between single nucleotide polymorphism (SNPs) in NF-Kb and the susceptibility as well as resolution of HCV infection. Patients and Methods: This prospective case-control study was conducted at the physical examination center on 150 Egyptian population, including 50 uninfected control cases, 50 cases with spontaneous viral clearance, and 50 cases with persistent HCV infection, they are genotyped for four SNPs (rs11820062, rs230530, rs1056890 and rs3774963) using a Taq Man assay. Results: The current study revealed that the mutation in rs_11820062 of the I kappa B alpha gene significantly increased the risk for HCV infection with a p-value <0.05. Conclusion: This study revealed that genetic variants of the NF-κB pathway genes (rs11820062 T allele) are associated with an increased risk of HCV susceptibility. light polypeptide gene enhancer","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83196130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stem cell therapy in renal diseases 肾脏疾病的干细胞治疗

Journal of Bioscience and Applied Research Pub Date : 2022-08-20 DOI: 10.21608/jbaar.2022.255500

Khalil Alhalfawy, Bahgat A. Elfiky, A. Zahran, Z. Kasemy, Mahmoud Zayed

引用次数: 0

Evaluation of Adiponectin hormone and some biochemical parameters with unstable angina and acute myocardial infarction patients 不稳定型心绞痛及急性心肌梗死患者脂联素激素及相关生化指标的评价

Journal of Bioscience and Applied Research Pub Date : 2022-07-28 DOI: 10.21608/jbaar.2022.251726

Alyaa Majid, Ş. Adem, Anwer Kadhim

引用次数: 0

Biochemical changes in Egyptian patients infected with COVID-19 埃及新型冠状病毒感染患者的生化变化

Journal of Bioscience and Applied Research Pub Date : 2022-07-28 DOI: 10.21608/jbaar.2022.251729

A. El-Adly, A. Wardany, Mohey Shikhoun

{"title":"Biochemical changes in Egyptian patients infected with COVID-19","authors":"A. El-Adly, A. Wardany, Mohey Shikhoun","doi":"10.21608/jbaar.2022.251729","DOIUrl":"https://doi.org/10.21608/jbaar.2022.251729","url":null,"abstract":"A pandemic-scale outbreak of the newly discovered coronavirus disease 2019 (COVID-19), fast-spreading viral pneumonia, is currently occurring. Due to the disease's overall vulnerability, different age groups have different clinical characteristics and test findings. The purpose of this study was to describe the COVID-19 laboratory results in various age and sex groups. Reverse transcriptase polymerase chain reaction (RT-PCR) for SARS-2 RNA was used in the study, which had 1100 individuals with typical cold symptoms. It was reported that 660 of these cases tested positive for the test, while 440 tested negatives, therefore all cases underwent laboratory testing. Our research revealed that males had higher COVID-19 positivity than females (215/660; 67.4%), with males scoring 445/660; 32.6%). Age does not statistically differ between COVID-19 positive and negative cases. Hematological parameters in blood cells revealed that Lymphocytes differ significantly between COVID-19-infected and uninfected patients as these cells decline in the presence of COVID-19 infection. There are no significant differences in hemoglobin (Hgb percent), red blood cells (RBCs), total white blood cells (WBCS), basophils, neutrophils, monocytes, and eosinophils, as well as blood platelets (PLTS). Erythrocyte sedimentation rate (ESR) is unimportant, whereas COVID-19 infection increases ferritin and C-reactive proteins.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80109140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Serum Ferritin in Type-2 Diabetes Mellitus Sudanese Patients 苏丹2型糖尿病患者血清铁蛋白的评价

Journal of Bioscience and Applied Research Pub Date : 2022-07-04 DOI: 10.21608/jbaar.2022.247840

Hayat Ahmed, N. Ali

{"title":"Evaluation of Serum Ferritin in Type-2 Diabetes Mellitus Sudanese Patients","authors":"Hayat Ahmed, N. Ali","doi":"10.21608/jbaar.2022.247840","DOIUrl":"https://doi.org/10.21608/jbaar.2022.247840","url":null,"abstract":": Background: Type 2 diabetes mellitus (DM2) is an important health problem worldwide affecting about 8 percent of the population. Diabetes Mellitus is one of the most common chronic diseases in Sudan. These patients have many changes in their blood, and many factors affect the glucose tolerance that is mediated by insulin, one of these changes is S.ferritin level, a marker of iron storage. Increased serum ferritin, reflecting body iron overload, is often associated with insulin resistance. The role of iron in the pathogenesis of diabetes is suggested by an increased incidence of type2 diabetes mellitus in diverse causes of iron overload, and reversal or improvement in glycemic control with a reduction in iron load achieved using either phlebotomy or iron-chelating therapy. Aim: To evaluate serum ferritin level in type-2 Diabetes Mellitus Sudanese Patients. Materials and Method: A case-control study was done in 45diabetic patients and 45normal healthy individuals as a control group, including measurement of serum ferritin by an electrochemiluminescence immunoassay (ECLIA) method full automated chemical analyzer. COBAS e411 machine used Roche HITACHI Kit and quantification of HbA1c level by i-chroma instrument. Results: The study showed that serum ferritin levels were significantly increased in diagnosed cases of type 2 diabetes mellitus in comparison with the age and gender-matched healthy individuals (P. value= 0.008). There was an insignificant correlation between serum ferritin and HbA1c(P. value=0.431). Conclusion: Therefore, the findings of the present study indicate that serum ferritin was increased in diabetes and this increase may contribute to the pathogenesis of this disease as well as to the development of complications. Thus, routine screening for serum ferritin concentration in pre-diabetes and diabetic patients should be done to assess the body's iron stores.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83167980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Case Report of Lymphoepithelioma-like, a Variant of Urothelial Carcinoma of the Urinary Bladder 淋巴上皮瘤样膀胱尿路上皮癌1例报告

Journal of Bioscience and Applied Research Pub Date : 2022-06-17 DOI: 10.21608/jbaar.2022.244515

Farouk Hachem, H. Abdallah, A. Ibrahimi, H. Elsayegh, Y. Nouini

引用次数: 0

Evaluation of the diagnostic performances of tissue inhibitors of metalloproteinase-1 and fibronectin for heart failure 金属蛋白酶-1和纤维连接蛋白组织抑制剂对心力衰竭诊断价值的评价

Journal of Bioscience and Applied Research Pub Date : 2022-06-01 DOI: 10.21608/jbaar.2022.246476

M. Fouda, B. Zarif, Victoria Samir, Sara A Mekkawy, Mohamed Omran

{"title":"Evaluation of the diagnostic performances of tissue inhibitors of metalloproteinase-1 and fibronectin for heart failure","authors":"M. Fouda, B. Zarif, Victoria Samir, Sara A Mekkawy, Mohamed Omran","doi":"10.21608/jbaar.2022.246476","DOIUrl":"https://doi.org/10.21608/jbaar.2022.246476","url":null,"abstract":"Previous research has linked an imbalance of the tissue inhibitors of metalloproteinase-1 (TIMP-1) and fibronectin (FN) to heart failure as a part of the extracellular matrix network (ECM) biochemistry profile, which is vital for cardiac homeostasis. This study aimed to assess the diagnostic performance of FN, TIMP-1, and CK-MB in heart failure (HF). Sixty patients (45 with acute and 15 with chronic HF) were recruited. Thirty individuals (20 with ischemic heart diseases, as other cardiac diseases, and 10 healthy individuals) were recruited as a control group. The biotin double antibody sandwich technology determined levels of human fibronectin and tissue inhibitors of metalloproteinase-1. FN was the most effective biomarker in differentiating HF patients from healthy individuals (AUC = 0.850) ( P < 0.001), followed by TIMP (AUC = 0.74) and CK MB (AUC = 0.660). The sensitivity and specificity of FN were 82% and 70%, respectively, at a cutoff of 80 ng/ml. In addition, FN and TIMP had the same AUC (0.71) and efficiency (65%) in distinguishing HF patients from controls, followed by CK-MB (AUC = 0.70). We developed a novel model for HF diagnosis named the HFD model based on three biomarkers (FN, TIMP, and CK MB). The HFD model had an AUC of 0.77 in distinguishing HF patients from healthy individuals, with a sensitivity, specificity, and accuracy reaching 80%. For differentiating HF patients from controls, the HFD model had 0.8 AUC, 76% sensitivity, 75% specificity, and 76% accuracy.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88819593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Role of CD3 and CD8 in Preterm Preeclamptic women by using Immunohistochemical technique 免疫组化技术研究CD3和CD8在早产子痫前期妇女中的作用

Journal of Bioscience and Applied Research Pub Date : 2022-06-01 DOI: 10.21608/jbaar.2022.246929

A. Abbood

引用次数: 0