Journal of Bioscience and Applied Research最新文献

{"title":"XANTHOGRANULOMATOUS PROSTATITIS","authors":"Farouk Hachem, I. Boualaoui, Ali Mikou, Zouaidia Fouad, A. Ibrahimi, H. Elsayegh, Y. Nouini","doi":"10.21608/jbaar.2022.246744","DOIUrl":"https://doi.org/10.21608/jbaar.2022.246744","url":null,"abstract":"Xanthogranulomatous prostatitis is a rare chronic inflammatory condition and may simulate prostatic carcinoma both clinically and microscopically. Conservative treatment is recommended for xanthogranulomatous prostatitis, including the use of alpha-blockers and corticosteroids. Surgical options, such as transurethral prostatic resection or open adenomectomy, are reserved for patients who are severely symptomatic or who have failed conservative treatment.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"119 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76712058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatic ameliorative role of Grape Seed Proanthocyanidin Extract against Ehrlich solid carcinoma-induced liver toxicity","authors":"A. Hasan, Haneen A. Hameed","doi":"10.21608/jbaar.2022.237709","DOIUrl":"https://doi.org/10.21608/jbaar.2022.237709","url":null,"abstract":"","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75487683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frequency and specificity of red blood cells alloantibodies among Sudanese multiparous women","authors":"N. Abdelateif, Abather Mohamed","doi":"10.21608/jbaar.2022.229094","DOIUrl":"https://doi.org/10.21608/jbaar.2022.229094","url":null,"abstract":": Background: Allo-immunization is a condition in which the body gains immunity, from another individual of the same species, against its cells. Blood group antibodies are called alloantibodies if they react with alloantigens on the red blood cells (RBCs) of other individuals. Immune antibodies are produced following exposure to foreign erythrocyte antigens through pregnancy or transfusion. Maternal alloimmunization, also known as iso-immunization, occurs when a woman's immune system is sensitized to foreign erythrocyte surface antigens, stimulating the production of immunoglobulin G (IgG) antibodies. Purpose: This study aimed to detect the frequency and specificity of alloantibodies against (RBCs) antigens among Sudanese multiparous women. Methods: This cross-sectional study was conducted on randomly selected 130 pregnant ladies, most of them were attending Soba teaching hospital and the remaining to Khartoum teaching hospital during the period from February-2008 to May-2008. Serum from each lady was collected. Each sample was grouped and screened for alloantibodies, then samples with positive antibody screening were tested for antibody identification using the gel agglutination method. Results: The overall frequency of alloantibodies detected among studied pregnant ladies was 10.8%. The results also revealed that an insignificant correlation was found between age, ethnic group, and presence of alloantibodies (( P-value : > 0.05); While a significant correlation was observed between the history of abortion, and the number of pregnancies and presence of alloantibodies ( P-value : < 0.05). Conclusion: In summary, we concluded that the alloantibodies were detected in one-tenth of the studied subjects with a significant association between detected antibodies and the number of pregnancies .","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86889664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of renal cell line apoptosis by hydrogen peroxide","authors":"Khalil Alhalfawy, Bahgat A. Elfiky, A. Zahran, M. Zayed","doi":"10.21608/jbaar.2022.228865","DOIUrl":"https://doi.org/10.21608/jbaar.2022.228865","url":null,"abstract":"Background: Hydrogen peroxide is currently the most widely used as an apoptosis inducer due to its broad cytotoxic efficacy against nearly all cell types. cytotoxicity is achieved over a wide range of doses Objective: To evaluate the cytotoxic effect of hydrogen peroxide on renal cell lines by detecting RIPK1. Methods: In this study, we used a Vero cell line treated with H 2 O 2 at concentrations 0.1 mM, 0.2 mM, 0.4 mM, 0.8 mM, and 1.6 mM and examined after 30 min,1hour,2hours,3hours, 4hours and 5 hours. by using MTT assay to detect cytotoxicity to cell line (by detecting cell viability). Spectrophotometrically measure the absorbance at a wavelength of 570 nm. Measure the background absorbance of multi-well plates at 690 nm and subtract from the 450 nm measurement. Sub-lethal dose to renal cell line is one treated with 1.6 mM for 5 hours. groups group 1: renal cell line as control which not treated by H2O2. group 2: Sub-lethal which renal cell line treated by concentration 1.6 mM of H2O2 for 5 hours. Results: H2O2 is cytotoxic to renal cell line by concentration from 0.1 mM to 1.6 mM. RIPK1 gene expressed in renal cell line treated by H2O2. The sublethal dose reached 1.6 mM for 5 hours. There is a significant difference between the 2 groups by detecting the expression of the RIPK1 gene.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"72 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91422601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association of the -158 XmnI γG globin polymorphism with HbF level in sickle cell anemia Sudanese Patients","authors":"R. Mohammed, N. Ali","doi":"10.21608/jbaar.2022.225891","DOIUrl":"https://doi.org/10.21608/jbaar.2022.225891","url":null,"abstract":"Background: Sickle cell hemoglobinopathy is a genetic disorder caused by the presence of hemoglobin S (HbS), γG-158 (C→T) polymorphism plays an important function in the disease severity of sickle cell anemia, The XmnI restriction site at -158 position of the γG-gene is associated with increased expression of the γG-globin gene and higher production of HbF, Previous studies have suggested that a variety of genetic determents influence different clinical phenotypes. The genetic variants that modulate HbF levels have a very strong impact on ameliorating the clinical phenotype. Aim: This study aims to associate between Xmn1 (...γG-158 C→T ...) polymorphism and fetal hemoglobin level among Sudanese patients with SCA.Materials and methods: In this descriptive crosssectional study 60 blood samples from diagnostic cases were analyzed using a Hematology analyzer (Sysmex KX21N), capillary electrophoresis (MINICAP), using “G-spinTM Total DNA Extraction Kit”, PCR-RFLP techniques. Results: Patients with SCA were analyzed for Xmn1 polymorphism and association between this polymorphism and severity of SCA was evaluated, the presence of one XmnI (+/-) site CT 2% in SS patients compared with XmnI-/site CC98% had shown difference regarding HbF level, thus the Polymorphic association was founded. Conclusion: In our descriptive cross-sectional study we concluded that the effect of the polymorphism on the Hb F level was established.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"270 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90957376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effects of Ambrosia maritima and Allium sativum Plant Extracts on Different Tissues of Envenomed mice with Leiurus quinquestriatus Scorpion Venom","authors":"N. Emam, Aljohara Al-Otabi","doi":"10.21608/jbaar.2022.224732","DOIUrl":"https://doi.org/10.21608/jbaar.2022.224732","url":null,"abstract":"","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81308053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"8-hydroxy-2'-deoxyguanosine and TP53 in Egyptian Patients with Hepatitis C Viral Chronic Liver Diseases: Insight into the Pathogenesis and Predictive Force","authors":"H. El-emshaty, Somaia Osman, F. El‐Taweel, M. El-Hemaly, H. Ismail","doi":"10.21608/jbaar.2022.223518","DOIUrl":"https://doi.org/10.21608/jbaar.2022.223518","url":null,"abstract":"Hoda M. El-Emshaty, Somaia M. Osman , Fathy M. El-Taweel, Mohamed M. El-Hemaly, Hisham Ismail 3 Gastrointestinal Surgery Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt Chemistry Dept., Faculty of Science, Damietta University, New Damietta, Egypt. Biochemistry Division, Chemistry Dept., Faculty of Science, Minia University, Minia, Egypt. Running title: Predictive force of serum 8-OHdG in HCV-chronic liver diseases *Corresponding Author: Dr. Hoda Mohamed El-Emshaty Professor of Biochemistry, Gastrointestinal Surgical Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt E-mail: elemshaty_h@yahoo.com DOI: 10.21608/jbaar.2022.223518 Abstract Reactive oxygen species (ROS) is excessively generated during tumor development yielding the oxidatively modified products of proteins and DNA. These DNA alterations could contribute to the development of cancer through the activation of oncogenes and inactivating tumor suppressor genes (TSGs). Therefore, 8-OHdG DNA oxidative damage and TP53 protein expression were evaluated amongst HCV-Chronic liver disease patients to explore their possible role in hepatocarcinogenesis and to predict HCC development at early stages. A total of 141 patients with HCV-related liver diseases; 69 with hepatocellular carcinoma and 72 with liver cirrhosis were enrolled in this study in addition to 56 healthy subjects. Serum 8-OHdG and TP53 expression by ELISA were markedly elevated in HCC patients compared to LC and healthy individuals (p<0.0001). A significant correlation was noted for 8-OHdG and TP53 with disease progression and tumor differentiation but not with tumor site. 8-OHdG and TP53 were highly (p<0.05) predicting for HCC at early stages and the diagnostic performance for discriminating HCC from LC by ROC curve showed the best AUC was recorded for 8-OHdG (0.745) followed by TP53 (0.667) with accuracy (87.2% and 82% respectively). Therefore, HCV-induced oxidative DNA damage could increase the carcinogenic potential of HCC development through the activation of TP53.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84242289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Folic Acid Conjugated Graphene Oxide Graviola Nanoparticle for Sono-Photodynamic Leukemia Treatment: Up-To-Date Cancer Treatment Modality","authors":"Mahmoud Matar Mohammad Abu Rakhey, Samir Ali Abd El-Kaream, D. Ma","doi":"10.21608/jbaar.2022.223360","DOIUrl":"https://doi.org/10.21608/jbaar.2022.223360","url":null,"abstract":"Sono-Photodynamic Leukemia Treatment: Up-To-Date Cancer Treatment Modality Mahmoud Matar Mohammad Abu Rakhey*, Samir Ali Abd El-Kaream, Daoxin Ma Qilu Hospital, China niversity Department of Hematology, Shandong U 1 Department of Applied Medical Chemistry, Alexandria University-Medical Research Institute, Egypt , * 1 akhey Mahmoud Matar Mohammad Abu R : nding author Correspo * Department of Hematology, Shandong University-Qilu Hospital, China Mahmoud@mail.sdu.edu.cn : mail institutional e ORCID iD: https://orcid.org/0000-0001-5318-5051","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83909685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In utero exposure of green coffee extract alters rat fetal neurodevelopment in a dose dependent manner","authors":"Marwa N. Atallah, Amira S. Abd El-Gaber","doi":"10.21608/jbaar.2022.222520","DOIUrl":"https://doi.org/10.21608/jbaar.2022.222520","url":null,"abstract":"a Vertebrates, Comparative Anatomy, and Embryology, Zoology Department, Faculty of Science-Menoufia University, Egypt. b Chemistry Department, Faculty of Science, Menoufia University, Egypt Correspondence: Dr. Marwa Atallah, Lecturer of Vertebrates Embryology and Comparative Anatomy – Zoology Department – Faculty of Science – Menoufia University – Egypt. Email: Marwa.atallah@science.menofia.edu.eg Tel: +201022664113","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"592 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78514928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The prognostic value of elevated creatine kinase as an Independent Prognostic Factor for COVID-19 mild infection","authors":"S. Alabd, Ahmed Mahmoud","doi":"10.21608/jbaar.2021.237395","DOIUrl":"https://doi.org/10.21608/jbaar.2021.237395","url":null,"abstract":": Background: Creatine kinase (CK), which is a marker of muscle damage, was also often found to be elevated in deceased COVID-19 patients and those with severe disease, Therefore, higher CK could be used as a prognostic marker to indicate a more severe clinical picture of COVID-19. Patients and methods: This study included 225 out-hospitalized patients with confirmed COVID-19 infection, these study subjects were randomly selected irrespective of the age group and both genders were included, Assay procedure as manufactory instructions and Reagents of Spectrum diagnostics, optimized DGKC/IFCC liquid reagent, Egypt are used. Results: This study showed male gender was more frequent (n=135, 60%) than the female gender (n=90, 40%). This study reveals high serum CK levels in 45 patients (20%), which has a sensitivity of 20% as a biomarker for COVID-19 diagnosis in out-hospitalized patients (Outpatients and patients under home observation), with a p-value is <0.001 which is less than 0.05. Conclusion: CK has a sensitivity of 20% in out-hospitalized patients (Outpatients and patients under home observation), thus elevated CK is best used as a rule-in test rather than a rule-out test.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"448 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82908789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}