Journal of Ayurveda and Integrative Medicine最新文献

{"title":"Impact of one week of Ayurveda-based therapy, lifestyle and diet modification in heart failure patients with reduced ejection fraction: A retrospective study","authors":"Rohit Sane ,&nbsp;Rahul Mandole ,&nbsp;Gurudatta Amin ,&nbsp;Tejashree Jadhav ,&nbsp;Prafull Yashwantrao ,&nbsp;Snehal Dongre ,&nbsp;Swapnil Srivastav","doi":"10.1016/j.jaim.2024.101083","DOIUrl":"10.1016/j.jaim.2024.101083","url":null,"abstract":"<div><h3>Background</h3><div>Management of Chronic Heart Failure (CHF) is quite challenging, and its prognosis remains poor. Thus, alternative and conventional treatment approaches with lifestyle modifications are needed to enhance the quality of life. One such approach is heart failure reversal treatment (HFRT) that includes Snehana (massage), Swedana (fomentation therapy), Basti (per rectal drug administration), and Hridaydhara.</div></div><div><h3>Objective</h3><div>The present study evaluated the effect of HFRT in CHF patients with heart failure with reduced ejection fraction (HFrEF), and relate the clinical benefit and change in hemodynamic parameters intending to improve the quality of life.</div></div><div><h3>Methods</h3><div>This study evaluated data from known CHF patients who visited the Madhavbaug Hospital between May 2021 to October 2021. Patients of either gender with NYHA Class I, II, III and LVEF&lt;40 who completed 7-day (12 sessions, one each at day 1 and 7 and remaining days 2 sessions/day) HFRT therapy were included in the study. Data on blood pressure, abdominal girth (ABG) and cardiac parameters [End diastolic volume (EDV), Ejection factor (EF), Systemic vascular resistance (SVR), 6-min walk test (6 MW T), etc. was analysed pre and post-HFRT.</div></div><div><h3>Results</h3><div>85 CHF patients with a mean age of 57 years and 67 (78.8%) males were enrolled in the study. The calculated EF, 6 MW T, stroke volume, and SVR significantly changed (p &lt; 0.05) on Day 7 post-HFRT intervention. Though, BMI, ABG, EDV, SV and CO showed a positive change, it was not statistically significant.</div></div><div><h3>Conclusion</h3><div>HFRT demonstrated a significant change in the hemodynamics, which was also impacted positively by the improvement in 6 MW T, thereby enhancing the quality of life.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 2","pages":"Article 101083"},"PeriodicalIF":1.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Vineeta V. Deshmukh","doi":"10.1016/j.jaim.2024.101090","DOIUrl":"10.1016/j.jaim.2024.101090","url":null,"abstract":"","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 2","pages":"Article 101090"},"PeriodicalIF":1.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro anti-inflammatory and in silico anti-viral assessment of phytoconstituents in polyherbal Ayurvedic formulation ‘Arogyamrita Kwath’","authors":"Akash Dey ,&nbsp;Akash G. Kendre ,&nbsp;Madhu babu Dande ,&nbsp;Yogita B. Tandalekar ,&nbsp;Shreyanshi Kulshreshtha ,&nbsp;Amit Srivastava ,&nbsp;Alok Goyal ,&nbsp;Ramadas Maganti ,&nbsp;Sumit Srivastava ,&nbsp;Prasad V. Bharatam ,&nbsp;Sanjay M. Jachak","doi":"10.1016/j.jaim.2024.101076","DOIUrl":"10.1016/j.jaim.2024.101076","url":null,"abstract":"<div><h3>Background</h3><div>Arogyamrita Kwath (AMK) is a polyherbal decoction comprising ten medicinal plants, <em>viz</em>., <em>Albizia lebbeck</em>, <em>Andrographis paniculata</em>, <em>Tinospora cordifolia</em>, <em>Adhatoda vasica</em>, <em>Solanum xanthocarpum</em>, <em>Curcuma longa</em>, <em>Glycyrrhiza glabra</em>, <em>Terminalia bellirica</em>, <em>Withania somnifera</em> and <em>Trachyspermum ammi</em>. The plants of the AMK formulation are traditionally used for the treatment of inflammation and respiratory ailments, but no scientific evidence has been reported so far for this formulation.</div></div><div><h3>Objectives</h3><div>To evaluate anti-inflammatory activity of AMK formulation <em>in vitro</em> and its fractions and to predict <em>in silico</em> anti-viral activity of identified potential phytoconstituents.</div></div><div><h3>Materials and methods</h3><div>The MTT cell cytotoxicity assay, nitric oxide (NO) inhibition assay and cytokines assay were carried out at concentrations 100 and 200 μg/mL. The phytoconstituents were identified by UPLC-PDA and UPLC-HRMS analyses. For pharmacoinformatics study molecular docking and molecular dynamics methods were used.</div></div><div><h3>Results</h3><div>The study revealed that AMK significantly inhibited NO in comparison to dexamethasone (100 μg/mL) and pro-inflammatory cytokines in RAW264.7 cells. The three fractions, <em>n</em>-hexane, EtOAc and <em>n</em>-BuOH prepared from the AMK formulation were non-cytotoxic against RAW264.7 murine macrophage cells during MTT cytotoxicity assay and showed satisfactory results during cytokines assay. Ethyl acetate fraction contains active phytoconstituents in appreciable quantities. 16 phytoconstituents have been identified by UPLC-HRMS analysis in the formulation and four phytocompounds were quantified by UPLC-PDA. Molecular dynamics study helped in identifying two macromolecular targets (viral replicase and the membrane protein), which are relatively more important.</div></div><div><h3>Conclusion</h3><div>In the present study, anti-inflammatory activity of AMK was evaluated and the claimed anti-viral property was re-confirmed by molecular modelling in this work. The results clearly established that AMK showed remarkable anti-inflammatory and anti-viral activities.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 2","pages":"Article 101076"},"PeriodicalIF":1.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143621243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ayurveda management of post TURP urethral stricture: A case report","authors":"T. Sushendra ,&nbsp;Foram P. Joshi ,&nbsp;Hetal L. Nakrani ,&nbsp;Manisha Kapadiya ,&nbsp;Saurabh P. Patel","doi":"10.1016/j.jaim.2024.101084","DOIUrl":"10.1016/j.jaim.2024.101084","url":null,"abstract":"<div><div>Post TURP (Trans Urethral Resection of Prostate) Urethral stricture incident rates have been reported between 2.2 and 9.8%. Weak urine stream, recurrent urethral infections, dysuria, retention of urine, etc. are the chief presenting complaints of the clinical cases. Sushruta described 12 types of <em>Mootraghata</em> (∼clinical conditions stating obstructive uropathies). Lower Urinary Tract Symptoms (LUTS) have been provided with separate diagnostic notation with its description in <em>Mootraghata</em>. Sushruta narrated <em>Mootrotsanga</em>-a clinical condition confirming obstruction of urine in the lower urinary tract developing LUTS. Sushruta recommended <em>Uttarabasti</em> (intra-urethral douching) as a line of treatment to get rid of obstructive uropathies of various origins. This case is about a 70-year-old nondiabetic, nonhypertensive male who reported a 2-month history of weak urinary stream, burning micturition, and increased micturition frequency. The patient was diagnosed with stricture at the distal penile urethra near the external urethral meatus and stricture at the junction of the middle and posterior urethra. This case was managed by, <em>Sadhyo Virechana</em> (Therapeutic purgation)<em>,</em> followed by <em>Uttarbasti</em> (intra-urethral douche) procedure with <em>Jatyadi</em> oil and other adjuvent Ayurveda medicaments like <em>Eranda Bhrishta Haritaki, Gokshuradi Guggul</em> and <em>Varunadi Kwatha</em> for 21 days. Encouraging outcome was achieved in terms of complete reversal from Urethral stricture and evident with Ascending Urethrogram (AUG) report. Since the last 1 years' follow-up, the patient has been living symptom-free. LUTS and especially, stricture pathologies can be managed through safe and noninvasive procedures like <em>Uttarabasti</em>.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 2","pages":"Article 101084"},"PeriodicalIF":1.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143637662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroencephalogram analysis on alpha/beta and theta/beta ratios due to shirodhara","authors":"Yogeshwar Dasari ,&nbsp;Lakshmana Rao Chebolu ,&nbsp;Venkatesh Balasubramanian","doi":"10.1016/j.jaim.2024.101094","DOIUrl":"10.1016/j.jaim.2024.101094","url":null,"abstract":"<div><h3>Background</h3><div><em>Shirodhara</em> is a brain relaxation therapy. The treatment induces a relaxed state with awareness that results in treating neurological conditions like mental stress (depression/anxiety/hypertension) and some of its beneficial side effects are soothing the central nervous system.</div></div><div><h3>Objectives</h3><div>In the present study, the investigation aimed to analyze the ratios of brain waves, particularly focusing on the frontal region.</div></div><div><h3>Materials and Methods</h3><div>In this study, <em>Shirodhara</em> with “<em>Ksheerabala thailam</em>” medicinal oil was conducted on 16 participants and pre-post treatment brain signals were collected using an EEG acquisition tool and it was found that treatment induced relaxation aspects were observed among participants.</div></div><div><h3>Results and Conclusion</h3><div>Welch's Fast Fourier transform (FFT) was used to analyze the EEG signals in order to obtain power spectral density (PSD) features, which indicated a signal's power dispersed across a range of frequencies. The effectiveness of treatment has been evaluated by examining the spectral power ratios of alpha/beta, theta/beta in the frontal region of the brain and physiological and psychological parameters.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 2","pages":"Article 101094"},"PeriodicalIF":1.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143637663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Randomized double blind placebo controlled multicentric clinical trial of Ayush M-3 in the management of migraine- A study protocol","authors":"Shweta Chaudhary ,&nbsp;Shruti Khanduri ,&nbsp;Sunita Mata ,&nbsp;B.C.S. Rao ,&nbsp;Kuljeet Singh Anand ,&nbsp;R. Kishore Kumar ,&nbsp;K.G. Sudha Kumari ,&nbsp;Bidhan Mahajan ,&nbsp;Rakesh Kumar Rana ,&nbsp;Richa Singhal ,&nbsp;Shweta Mata ,&nbsp;Renu Makhija ,&nbsp;Bharti ,&nbsp;N. Srikanth ,&nbsp;K.S. Dhiman ,&nbsp;Rabinarayan Acharya","doi":"10.1016/j.jaim.2024.101054","DOIUrl":"10.1016/j.jaim.2024.101054","url":null,"abstract":"<div><h3>Background</h3><div>Migraine is acknowledged as an intricate and incapacitating neurological malady, profoundly exerting a potent influence on the health-related quality of life of patients causing disability, and augmented burden on families, and entire communities. Notwithstanding the strides made in the field of headache disorder management, there remains a cohort of migraine-afflicted patients who do not encounter satisfactory alleviation. This emphasizes the need for alternative systems in addressing the issue and there is a growing interest in exploring Indian system of medicine to address migraine symptoms. <strong>Objective:</strong> Due to the dearth of prospective, high-quality randomized controlled trials, this protocol aims to conduct a scientific study of the polyherbal formulation ‘Ayush M3’ to assess its safety and efficacy in patients suffering with migraine.</div></div><div><h3>Materials and Methods</h3><div>A prospective, randomized, double blind, parallel-group, placebo controlled, multi – centric, two-arm, exploratory study has been designed to enrol a total of 360 patients at three sites. Migraine patients with or without aura diagnosed as per International Headache Society-ICHD-III below Grade III of MIDAS will be randomized in a 1:1 ratio and administered the trial drug, Ayush M − 3 or placebo for twice a day after food for 16 weeks.</div></div><div><h3>Outcome measures</h3><div>The primary outcomes measures for the study will be changes in the character of disease in terms of frequency, intensity of headache as per VAS (0–10) and duration of episode of headache. Secondary outcome measure will be changes in MIDAS, MSQ2.1, changes in associated symptoms of migraine, change in the use of analgesics/symptom alleviating medication &amp; Change in the required dose/withdrawal of Anti-hypertensive drugs if any. Assessment will be conducted on an interval of 28 days till 16 weeks and an intervention free follow up at the end of week 20.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 2","pages":"Article 101054"},"PeriodicalIF":1.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A non-invasive Ayurveda management of venous leg ulccer- A case report","authors":"Archana Muraleedharan,&nbsp;Sree R. Nair,&nbsp;P.N. Rajeshwari","doi":"10.1016/j.jaim.2024.101073","DOIUrl":"10.1016/j.jaim.2024.101073","url":null,"abstract":"<div><div>Venous leg ulcer (VLU) is a severe complication of chronic venous insufficiency that commonly affects older individuals. It is a long-standing consequence of venous insufficiency and accounts for 70%–80% of lower limb ulcers seen in outpatient departments. These ulcers are often unresponsive to treatment and can significantly impact patients' quality of life, causing pain, sleep disturbances, and decreased mobility. Managing varicose leg ulcers is a significant challenge for physicians.A 55-year-old male presented with symptoms including pain, stiffness, discoloration, swelling in the left lower limb, and a history of non-healing ulcer. The patient was treated with <em>Patrad</em><em>a</em><em>na</em> using leaves of <em>Ricinus communis</em> (<em>Eranda patra</em>) and <em>Grihadhum</em><em>a</em><em>di lepa</em> along with oral medications such as <em>Triphal</em><em>a</em> <em>Guggulu</em> and <em>Gandhaka Ras</em><em>a</em><em>yana</em>. Each day, the change was carefully observed and documented, revealing a notable improvement by the end of the treatment (10 days). The pain, swelling, and stiffness exhibited significant reduction, accompanied by wound healing after the first follow-up of 14 days. The complete wound healing was achieved and quality of life showed improved. The patient has beenunder observation and has not reported any recurrence of ulcer in the 4 months of follow up.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 2","pages":"Article 101073"},"PeriodicalIF":1.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safe and effective management of psoriasis through Ayurveda: A case report","authors":"Guruprasad C. Nille ,&nbsp;Monisha Bhuyan ,&nbsp;Laxmi Narayan Gupta ,&nbsp;Anand Kumar Chaudhary","doi":"10.1016/j.jaim.2024.101091","DOIUrl":"10.1016/j.jaim.2024.101091","url":null,"abstract":"<div><div>Ayurveda, an ancient system, offers in-depth insights into various skin disorders. It provides detailed understanding of causative factors and pathomechanisms, along with time-tested traditional treatments based on its principles. We report a 16-year chronic case of psoriasis (36-year-old male patient) with plaques and peeling erythrodermic skin rashes over the back and front of the trunk area and both legs. The scalp area and nail beds were also affected. The early involvement of joints was also noted. A two-year multimodal Ayurveda treatment has shown notable efficacy in managing chronic plaque and erythrodermic psoriasis, with no reported adverse events or side effects. The progressive events were documented in the form of photographs with the proper consent of the patient. The Ayurveda medicines, strict dietary regimen, and regular follow-ups together resulted in promising outcomes, revalidating the potential of Ayurveda in controlling psoriasis and its complications.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 2","pages":"Article 101091"},"PeriodicalIF":1.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Yograj Guggulu, Ashwagandha Churna and Narayana Taila in management of Osteoarthritis Knee: A study in tribal dominant community","authors":"Kalpana Kachare ,&nbsp;Deepa Makhija ,&nbsp;Abha Sharma ,&nbsp;Sunita ,&nbsp;N. Srikanth ,&nbsp;B.S. Sharma ,&nbsp;Rakesh Rana ,&nbsp;Arunabh Tripathi ,&nbsp;B.C.S. Rao ,&nbsp;Purnendu Panda ,&nbsp;Ekta ,&nbsp;Raghavendra Naik ,&nbsp;K. Nishanth ,&nbsp;U.R. Shekhar Namburi ,&nbsp;Vimal Tiwari ,&nbsp;S.B. Singh ,&nbsp;Monika ,&nbsp;P. Srinivas ,&nbsp;Vipin Sharma ,&nbsp;Anil Avhad ,&nbsp;Abhaydev","doi":"10.1016/j.jaim.2024.101077","DOIUrl":"10.1016/j.jaim.2024.101077","url":null,"abstract":"<div><h3>Background</h3><div>Osteoarthritis (OA) is the most prevalent joint disease and a major cause of joint impairment and physical debility, common in elderly, women and laborious workers. The available non-steroidal anti-inflammatory drugs (NSAID) are being prescribed to manage the condition; however, newly discovered alternatives are looked upon by the practitioners. Thus, the study was aimed to provide an effective and safer alternative through Ayurveda for the management of OA.</div></div><div><h3>Objective</h3><div>To document the role of the selected Ayurveda formulations in the management of OA and to assess the tolerability of the formulations.</div></div><div><h3>Material and methods</h3><div>It was an open-label, multicentric, single-arm, prospective, study conducted at 14 peripheral institutes of the Central Council for Research in Ayurvedic Sciences, New Delhi. 483 participants of any gender between the age 40 to 65 years diagnosed with OA knee as per the ACR diagnostic criteria (2012) and willing to provide consent were enrolled in the study. Oral administration of Ayurvedic formulations Yograj Guggulu, Ashwagandha Churna and the local application of Narayana Taila was given for 12 weeks and assessment was done by means of Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) Modified-CRD, Pune version Score, Visual Analogue Scale score and disease-specific symptoms on 28th, 56th, 84th and 112th day.</div></div><div><h3>Results</h3><div>Significant change (P&lt;0.001) was observed in WOMAC score, VAS score and cardinal symptoms of OA knee. No adverse events reported in the study and the study drugs were well tolerated by the participants.</div></div><div><h3>Conclusion</h3><div>The study substantiates that administration of Yograj guggulu, Ashwagandha Churna and Narayana Taila, is well acceptable and tolerable. The interventions effectively alleviate the cardinal symptoms of OA knee.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 2","pages":"Article 101077"},"PeriodicalIF":1.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143815166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting cell cycle arrest in breast cancer by phytochemicals from Caryto urens L. fruit ethyl acetate fraction: in silico and in vitro validation","authors":"Ghanshyam Parmar,&nbsp;Jay Mukesh Chudasama,&nbsp;Ashish Shah,&nbsp;Chintan Aundhia,&nbsp;Sunil Kardani","doi":"10.1016/j.jaim.2024.101095","DOIUrl":"10.1016/j.jaim.2024.101095","url":null,"abstract":"<div><h3>Background</h3><div><em>Caryota urens</em>, also known as Shivjata, has been documented in ancient Indian texts for its therapeutic benefits, addressing conditions from seminal weakness to gastric ulcers. This study aims to investigate its contemporary medicinal potential in treating breast cancer.</div></div><div><h3>Objectives</h3><div>The study focuses on exploring the therapeutic potential of <em>Caryota urens</em> fruit against breast cancer, specifically targeting cell cycle genes CDK1, CDC25A, and PLK1 through bioinformatics, network pharmacology, and in vitro validation.</div></div><div><h3>Materials and methods</h3><div>Using mass spectrometry and nuclear magnetic resonance (NMR), 60 key phytoconstituents from <em>Caryota urens</em> fruit were identified. Bioinformatics analysis, integrating Gene Cards and GEO databases, 15,474 breast cancer-associated genes focusing on the HR+/HER2-subtype were identified. Molecular docking and qPCR validated the interactions of key phytoconstituents, particularly Episesamin, with CDK1, CDC25A, and PLK1. In vitro studies were conducted on the MCF7 cell line, supplemented by ROC and survival analyses to evaluate diagnostic and therapeutic potential.</div></div><div><h3>Results</h3><div>The bioinformatics analysis identified CDK1, CDC25A, and PLK1 as pivotal genes regulating cell cycle progression and breast cancer tumorigenesis. Network pharmacology and in vitro studies indicated that phytoconstituents, especially Episesamin, downregulated these genes in breast cancer cells. Molecular docking and qPCR confirmed these interactions, and ROC and survival analyses underscored their diagnostic and therapeutic significance.</div></div><div><h3>Conclusions</h3><div>This study suggests that <em>Caryota urens</em> fruit extract, particularly Episesamin, may inhibit breast cancer metastasis by downregulating CDK1, CDC25A, and PLK1, offering promising new strategies for targeting the cell cycle in breast cancer and emphasizing the value of integrating bioinformatics with experimental methods in cancer research.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 2","pages":"Article 101095"},"PeriodicalIF":1.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143600897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}