Journal of Ayurveda and Integrative Medicine最新文献

{"title":"Cardiac rehabilitation through conventional care with add on Ayurveda intervention following acute myocardial infarction help improving the cardiac function - A case report","authors":"Sanjeev Rastogi,&nbsp;Shashwat Rastogi,&nbsp;Tarun Mittal","doi":"10.1016/j.jaim.2026.101353","DOIUrl":"10.1016/j.jaim.2026.101353","url":null,"abstract":"<div><div>Acute Myocardial Infarction (AMI) is a life threatening event and leads to lifelong consequences if the patient survives the event. In view of global hypokinesia and reduced ventricular ejection fractions, subsequent management requires pharmacotherapy and cardiac rehabilitation to improve outcomes. Cardiac rehabilitation is a multidisciplinary program involving exercise training, risk factor reduction, education and psychological support, besides standard pharmacotherapy. We present here a case of 46 year old male having AMI, survived with severe debility due to low ventricular ejection fraction. Ayurveda intervention besides conventional cardiac rehabilitation was added here for any possibility to improvise the results. Such an add-on and its impact in cardiac outcomes have not been reported in the literature so far.</div><div>The case was diagnosed as of Antero-septal MI (ASMI). Conventional interventions were done at a tertiary care hospital and upon stabilization patient was discharge with recommendations. After 3 months of initial event, in view of slow recovery, patient asked for Ayurveda interventions as add-on to the conventional care.</div><div>Conventional Cardiac rehabilitation with add on Ayurveda intervention for 8 months resulted in good recovery of the patient with an improved ventricular ejection fraction up to 50%. His debility was also much reduced and he was able to do his routine chorus with ease. No adversities were reported following the conventional cardiac post infarction care along with Ayurveda interventions.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"17 4","pages":"Article 101353"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148463141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interstitial cystitis with Hunner's ulcers managed with Jatyadi Taila Uttarabasti - A case series","authors":"Kaushal Patel,&nbsp;Manish Patel,&nbsp;Chintan Bhatt","doi":"10.1016/j.jaim.2026.101358","DOIUrl":"10.1016/j.jaim.2026.101358","url":null,"abstract":"<div><div>Interstitial cystitis (IC) with Hunner's ulcers is a chronic inflammatory condition often resistant to conventional therapies, which frequently offer only temporary relief or carry risks of bladder scarring. This report describes the clinical outcomes of <em>Jatyadi Taila Uttarabasti</em> (intravesical instillation) combined with <em>Virechana Karma</em> (purgation) and oral formulations—in managing severe IC.</div><div>Two patients with chronic IC and Hunner's ulcers, non-responsive to conventional treatments, received individualized Ayurvedic treatment at the hospital. Clinical progress was documented using the Visual Analogue Scale (VAS) for pain, the King's Health Questionnaire (KHQ), and the IC-Quality of Life (IC-QoL) score, with a follow-up period of two years.</div><div>Both patients demonstrated significant clinical improvement. Pain scores reduced from severe to near-zero, and urinary frequency and nocturia were normalized. Quality-of-life metrics showed substantial enhancement, with IC-QoL scores reflecting near-complete symptom resolution. Notably, these results remained stable throughout the two-year follow-up period. Patient 2 opted for a planned maintenance course in late 2024 to ensure clinical stability, despite the resolution of primary symptoms.</div><div>This case series suggests that Ayurvedic management, particularly the use of <em>Jatyadi Taila Uttarabasti</em> for its wound-healing and anti-inflammatory properties, provides a cost-effective, non-invasive, and long-lasting alternative for severe IC and Hunner's ulcers.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"17 4","pages":"Article 101358"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148584405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ayurvedic management of Irritable Bowel Syndrome (Grahani Roga): A case report","authors":"Mayuri M. Mungale,&nbsp;Ashish A. Thatere","doi":"10.1016/j.jaim.2026.101377","DOIUrl":"10.1016/j.jaim.2026.101377","url":null,"abstract":"<div><div>Irritable Bowel Syndrome (IBS) is a functional gastrointestinal disorder characterised by irregular bowel movements and abdominal discomfort in the absence of detectable structural abnormalities. This condition significantly affects both physical and mental health. Although the precise etiology remains unclear, factors such as abnormal gut motility and sensory function, central nervous system disturbances, psychological stress, and luminal changes are implicated. IBS affects approximately 10–15% of the population, yet only 10% of those affected seek medical care. The prevalence is highest between ages 15 and 50, ranging from 3% to 22%, with young women more frequently affected than men. Conventional management primarily involves dietary modification, pharmacotherapy, and stress reduction, but these approaches rarely provide complete relief. Evidence indicates increased mortality risks associated with antibiotics and antispasmodics, highlighting the need for adjunctive therapeutic strategies. In Ayurveda, IBS is analogous to <em>Grahani Roga</em>, which is characterised by <em>Muhurbaddha-muhurdrava Mala Pravritti</em> (alternate formed and loose stools), <em>Udara Shoola</em> (colicky abdominal pain), <em>Adhmana</em> (abdominal distention), and <em>Dourbalya</em> (weakness). This report presents a 71-year-old female with frequent evacuation of stools of varying consistency (4–5 times daily), abdominal pain, bloating, generalised weakness, and reduced sleep for three years. The diagnosis of <em>Grahani Roga</em> was established based on clinical features, and the Rome IV criteria confirmed IBS with predominant diarrhea. The patient experienced minimal relief from three years of conventional therapy. She subsequently received <em>Piccha Basti</em> (enema) for 16 days and <em>Shamana</em> (pacifying) medicine for four months. Improvements were observed on the IBS-GAI, IBS-SSS, IBS-AR, and IBS-QoL scales, suggesting the potential benefit of Ayurvedic interventions in the management of IBS.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"17 4","pages":"Article 101377"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148578659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Commiphora wightii and its formulations extenuate macrophage-mediated inflammatory pathology in osteoarthritis","authors":"Vanshika Srivastava,&nbsp;Ritik Raj,&nbsp;Ankit Ganeshpurkar,&nbsp;Priya Kulkarni,&nbsp;Abhay Harsulkar","doi":"10.1016/j.jaim.2026.101370","DOIUrl":"10.1016/j.jaim.2026.101370","url":null,"abstract":"<div><h3>Background</h3><div>Osteoarthritis (OA) treatment often focuses on symptom management rather than addressing underlying inflammation and cartilage degeneration. In search of safer, long-term options, many patients turn to Ayurvedic remedies like Guggul (<em>Commiphora wightii</em>) and its formulations—Amritadi Guggul (AG) and Rasnadi Guggul (RG)—though biochemical validation remains limited.</div></div><div><h3>Objective</h3><div>This study evaluates the immunomodulatory effects of standard guggulsterone extract (SGE), AG, and RG on synovial inflammation, mitochondrial stress, and complement activation, using the U937 monocyte cell line.</div></div><div><h3>Materials and Methods</h3><div>Cells were stimulated with Phorbol 12-myristate 13 acetate (PMA) and treated with varying concentrations of the extracts. Anti-inflammatory effects were measured via mRNA expression of iNOS, MMP-1, MMP-13, and VEGF-1. Macrophage polarization markers (CD68, CD86, CD163), mitochondrial membrane potential (JC-1 assay), collagenase activity (gelatinase spot assay), and molecular docking with complement factor B (CFB) were also assessed.</div></div><div><h3>Results</h3><div>Results showed that SGE, AG, and RG significantly reduced nitric oxide and pro-inflammatory gene expression. Treatments suppressed M1 macrophage markers without promoting M2 differentiation. 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimi- dazolylcarbocyanine iodide (JC) - 1 assays indicated improved mitochondrial stability, while all formulations inhibited collagenase activity. Docking studies revealed strong interactions between guggulsterone and CFB, suggesting complement inhibition.</div></div><div><h3>Conclusions</h3><div>These findings highlight the potential of Guggul and its formulations to modulate macrophage activity, reduce inflammation, and support joint preservation in OA. Though limited by the use of a monocyte-derived cell line, the study lays the groundwork for future validation in primary cells and in vivo models.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"17 4","pages":"Article 101370"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing Latent Dirichlet Allocation-based topic modeling to unveil the focus areas in Ayush healthcare in India","authors":"Tina Dutta,&nbsp;Abhinav Kumar","doi":"10.1016/j.jaim.2026.101356","DOIUrl":"10.1016/j.jaim.2026.101356","url":null,"abstract":"<div><div>The exponential growth of unstructured data has presented new opportunities for leveraging computational techniques to uncover meaningful insights in diverse domains, including healthcare. In the context of India's pluralistic medical ecosystem, the Ministry of Ayush plays a central role in shaping policies related to traditional systems such as Ayurveda, Yoga, Naturopathy, Unani, Siddha, and Homeopathy. Despite the increasing prominence of AYUSH, especially post-COVID-19, there has been limited scholarly engagement with its policy evolution through a data-driven lens. This study applies Latent Dirichlet Allocation (LDA), a topic modeling technique, to analyze annual reports published by the Ministry of Ayush from 2012 to 2023. The research aims to (i) identify recent focus areas in alternate healthcare policy, (ii) track temporal shifts in strategic priorities, and (iii) uncover underrepresented themes in institutional discourse. The findings reveal evolving trends such as increased emphasis on research, integration with mainstream health systems, digital initiatives, and global promotion. The study identifies four critical dimensions that remain underrepresented within Ayush policy discourse: the positioning of Ayush philosophy as a frontline public health framework, the articulation of an Ayush-centric health system paradigm, the programmatic integration of mental health, and the systematic incorporation of artificial intelligence beyond foundational digital health initiatives, thereby identifying areas requiring greater strategic attention and reform. By introducing topic modeling into Indian healthcare policy research, this paper fills a key methodological gap and offers empirical insights into the policy trajectory of Ayush. To the best of the authors' knowledge, this is the first application of LDA to Ayush's policy literature.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"17 4","pages":"Article 101356"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148591651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of Nadamay meditation: A sound-based protocol for psychosocial well-being","authors":"Km Megha,&nbsp;Arjun Ram Roj,&nbsp;Sanjib Patra","doi":"10.1016/j.jaim.2026.101375","DOIUrl":"10.1016/j.jaim.2026.101375","url":null,"abstract":"<div><h3>Background</h3><div>Psychosocial well-being is increasingly recognized as a crucial aspect of overall health, with stress, anxiety, and emotional dysregulation affecting individuals across various demographics. Meditation has been widely studied for its potential to enhance mental well-being. This study developed and validated the Nadamay meditation technique, a structured practice based on ancient <em>Nada</em> yoga principles from the Nada Bindu Upanishad.</div></div><div><h3>Objective</h3><div>To develop and validate the <em>Nadamay</em> meditation protocol, ensuring its comprehensiveness and content validity for promoting psychosocial well-being, including aspects of mental, emotional, and social health, across diverse populations.</div></div><div><h3>Methods</h3><div>The <em>Nadamay</em> meditation protocol was developed by incorporating the principles of the <em>Nada Bindu</em> Upanishad. 21 subject matter experts from various fields reviewed the seven meditation steps using the Content Validity Ratio (CVR) and Item-level Content Validity Index (I-CVI) methods.</div></div><div><h3>Results</h3><div>All seven steps demonstrated acceptable to excellent content validity, with CVR scores ranging from 0.52 to 1.0 and I-CVI scores from 0.76 to 1.0, exceeding critical thresholds. The Scale-level Content Validity Index Average (S-CVI/Ave) was 0.92, confirming excellent overall content validity and the protocol's comprehensiveness.</div></div><div><h3>Conclusion</h3><div>The Nadamay meditation protocol is a structured, content-validated intervention for psychosocial well-being. While preliminary validation is promising, its inclusion of energizing practices like <em>Kapalbhati</em> and its broad design necessitate further feasibility and safety testing before widespread implementation.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"17 4","pages":"Article 101375"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148591712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systemic Ama assessment and Clinical Disease Activity in patients with Amavata (Rheumatoid arthritis)","authors":"Shrikant Wagh,&nbsp;Vinayak Joshi,&nbsp;Ashwinikumar A. Raut","doi":"10.1016/j.jaim.2026.101317","DOIUrl":"10.1016/j.jaim.2026.101317","url":null,"abstract":"<div><div>Objective assessment of disease severity is necessary during management and clinical studies of <em>Amavata</em>. Various validated instruments are available for measuring disease activity in Rheumatoid arthritis (RA). A validated instrument for systemic <em>Ama</em> assessment in <em>Amavata</em> (RA) measures <em>Ama,</em> but does not determine the disease activity in these cases. The <em>Ama</em>-score did not correlate well with the disease activity score (DAS) or erythrocyte sedimentation rate (ESR). We, therefore, used another composite score, viz. Clinical Disease Activity Index (CDAI) to assess its correlation with the <em>Ama</em>-score in patients with <em>Amavata</em> (RA).</div><div>To evaluate the correlation between the <em>Ama</em>-score and CDAI in patients with <em>Amavata</em> (RA).</div><div>CDAI and <em>Ama</em>-score were evaluated in two groups of patients with <em>amavata</em> (RA), Group A: treatment-naive patients (n = 13) and Group B: patients on treatment (n = 25) who are in remission. <em>Ama</em>-score was also assessed in apparently healthy volunteers without any overt symptoms, particularly gastrointestinal (Group C; n = 20). Spearman Rank Correlation was used for correlation between variables. Mann-Whitney <em>U</em> Test and Kruskal-Wallis Test were used for inter-group comparison. <em>P</em> value &lt; 0.05 was considered significant.</div><div>The median <em>Ama</em>-score and CDAI in Group A were 44 (12–76.5) and 32 (24–43), and those in Group B were 2 (0–22) and 0 (0–2), respectively. The difference in the <em>Ama</em>-score (<em>P</em> = &lt; 0.0001) and CDAI (<em>P</em> = &lt; 0.0001) between Groups A and B is significant. There was no significant difference between Group B and Group C (P &gt; 0.05). There is a significant correlation between <em>Ama</em>-score and CDAI in these subjects (Spearman r 0.6447, <em>P</em> &lt; 0.0001; 95 % CI 0.4012–0.8031).</div><div>There is a good correlation between <em>ama</em>-score and CDAI. <em>Ama</em>-score and CDAI with additional joint assessment can be combined to develop an objective Ayurvedic tool that can be used for patient management and reporting clinical studies in <em>amavata</em> (RA).</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"17 4","pages":"Article 101317"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148584434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Management of De-Quervain's tenosynovitis by Agnikarma with Suvarana Shalaka: A case report","authors":"Poonam Garg,&nbsp;Manorma Singh,&nbsp;Suman Sharma,&nbsp;Rahul Sharma,&nbsp;Bharati Samota","doi":"10.1016/j.jaim.2026.101387","DOIUrl":"10.1016/j.jaim.2026.101387","url":null,"abstract":"<div><div>De-Quervain's tenosynovitis is a painful condition characterized by wrist pain and swelling at the radial styloid process due to inflammation and thickening of the tendon sheath of the abductor pollicis longus and extensor pollicis brevis. Conventional treatment includes rest, analgesics, corticosteroid injections, and surgical release. In Ayurveda, this condition is correlated with <em>Snayugata Vata</em>, where <em>Agnikarma</em> (therapeutic heat) is recommended. A 55-year-old female presented with severe left wrist pain, swelling and difficulty in performing household work for 20 days. Finkelstein's test result was positive, with tenderness of grade-3 (withdrawal sign) and visible swelling. She underwent three sessions of <em>Agnikarma</em> using <em>Suvarana Shalaka</em> (gold rod) in a linear <em>Bindu</em> (point) pattern at weekly intervals. Outcomes were assessed using the visual analogue scale (VAS), Quick disability of the Arm, Shoulder and Hand (Quick DASH) score, and soft tissue tenderness scale. Complete pain relief and functional recovery of the wrist were achieved and sustained over an eight-month follow up period. <em>Agnikarma</em> alleviates <em>Sroto-Avrodha</em>(obstruction) enhancing <em>Rasa-Raktadi Dhatu Samvahana</em> (nutrient flow) and tissue extensibility. <em>Suvarana Shalaka</em> delivers continuous therapeutic heat through the conduction method without causing <em>Dagdha Vrana</em> (burn or blisters). This case report serves as a lead for future researches on the efficacy of <em>Agnikarma</em> with <em>Suvarana Shalaka</em> on <em>Snayugata Vikara</em>. It also suggests <em>Agnikarma</em> is a safe, cost-effective, day-care, drug-less treatment that obviates reliance on long-term use of analgesics and anti-inflammatory drugs.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"17 4","pages":"Article 101387"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13393633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148548993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The systemic anti-diabetic effect of polyherbal formulation Varanadi Kashayam is mediated through GLP-1 secretion and DPP4 inhibition","authors":"Anjana Thottappillil,&nbsp;Sania Kouser,&nbsp;Abhi V. Badiger,&nbsp;Priyanka Gladys Pinto,&nbsp;Srimathy Ramachandran,&nbsp;Suresh Janadri,&nbsp;Manjunatha P. Mudagal,&nbsp;Subrahmanya Kumar Kukkupuni,&nbsp;S. Suma Mohan,&nbsp;Chethala N. Vishnuprasad","doi":"10.1016/j.jaim.2026.101359","DOIUrl":"10.1016/j.jaim.2026.101359","url":null,"abstract":"<div><h3>Background</h3><div>Frontiers of health science increasingly emphasize systems and network medicine approaches for managing complex lifestyle diseases such as diabetes. In this context, systemic targets like incretin hormones and their modulators, particularly DPP4 inhibitors have gained prominence. Ayurveda<em>,</em> the Indian System of Medicine, with its clinically validated multicomponent formulations, offers a valuable resource for integrative therapeutic strategies with systemic mode of action.</div></div><div><h3>Objectives</h3><div>The study investigates the incretin modulatory effect of <em>Varanadi Kashayam</em> (VA), an Ayurveda polyherbal formulation used in the clinical management of diabetes and its comorbidities.</div></div><div><h3>Methods</h3><div>A high-fat diet and low dose STZ-induced Sprague-Dawley (SD) rat model was used to study anti-diabetic, GLP-1 secretory, and anti-obesity effects of VA. <em>In vitro</em> studies using GLUTag cells assessed GLP-1 secretion and DPP4 gene expression modulation; and studies using 3T3-L1 fibroblasts examined anti-adipogenic effects. Computational methods including molecular docking and molecular dynamic simulations identified phytochemicals potentially contributing to DPP4 inhibition.</div></div><div><h3>Results</h3><div>VA administration significantly improved fasting blood glucose and oral glucose tolerance in experimental animals, along with enhanced GLP-1 secretion. <em>In vitro</em> results showed significant inhibition of DPP4 enzyme activity, increased GLP-1 secretion, and downregulation of DPP4 gene expression in GLUTag cells, and suppression of adipogenesis in 3T3-L1 fibroblasts. Computational analyses ascertained Chebulinic acid, Chebulagic acid, and Terchebin as top-ranked phytochemicals that could potentially inhibit DPP4.</div></div><div><h3>Conclusion</h3><div>Combining <em>in vitro</em>, <em>in vivo</em>, and <em>in silico</em> findings, this study provides valuable insight into the incretin modulatory effect of VA in treating diabetes and associated metabolic diseases.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"17 4","pages":"Article 101359"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148421128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multitarget anticancer profiling of Withania somnifera - Dual herbal combination with P. emblica, B. monnieri, and O. basilicum through integrated In silico docking and In vitro Jurkat cell line analysis","authors":"Bhargavi Srinivasan,&nbsp;S.R. Madhan Shankar","doi":"10.1016/j.jaim.2026.101373","DOIUrl":"10.1016/j.jaim.2026.101373","url":null,"abstract":"<div><h3>Background</h3><div>Combinatorial Ayurvedic Rasayana extracts represent a rich source of bioactive compounds with anticancer potential. <em>Withania somnifera</em> (W) and its dual herbal Rasayana partners—<em>Phyllanthus emblica</em> (WP), <em>Bacopa monnieri</em> (WB), and <em>Ocimum sanctum</em> (WO)—are traditionally used to restore immune function, enhance resilience, and improve systemic health. Their multi-targeted phytoconstituents provide a promising approach for cancer therapy.</div></div><div><h3>Objective</h3><div>This study aimed to identify bioactive compounds from <em>W. somnifera</em> and its dual <em>Rasayana</em> extracts, evaluate their <em>in silico</em> binding affinity with cancer-related targets, and assess their <em>in vitro</em> antiproliferative potential compared with cladribine<strong>.</strong></div></div><div><h3>Materials and methods</h3><div>Phytocompounds were identified by GCMS and pharmacological activities were predicted using PASS–BAS. Molecular docking (Schrodinger- Glide) against 23 cancer-relevant proteins curated from TCGA and COSMIC datasets. Drug-likeness and ADME were evaluated with QikProp 3.0 and SwissADME. Molecular Dynamics (MD) simulations (100 ns, Desmond) were conducted for selected protein-ligand complexes to validate binding stability through RMSD, RMSF, SSE, and protein-ligand interaction analysis. Jurkat E6-1 leukemia cells were treated with W, WP3, WB2, and WO3 extracts for 24 h, and antiproliferative activity assessed via MTT assay.</div></div><div><h3>Results</h3><div>Of 62 screened phytocompounds, four key ligands —Campesterol (W), WP ligand, WB ligand, and Retinol (WO)—based on drug-likeness, pharmacokinetics, and compared with Cladribine. Docking revealed high affinities: WP3 with FAAH (−10), WB2 with PPARD (−9.08) and RBP4 (−9.5), WO3 with PPARA (−9.85) and RBP4 (−10.23), and W with CYP17A1 (−8.74). Enrichment analysis linked these targets to MAPK, JAK–STAT, and PPAR pathways. The MD results of both the complexes (5NUJ-445354 and 4D03-9592405) showed acceptable stability with dynamic adaptability, supporting the robustness of docking predictions. WP3 and WO3 had the highest antiproliferative activity (IC₅₀ 20.27 and 20.40 μg/mL) against Jurkat cells at 24hr and at 25 μg/mL.</div></div><div><h3>Conclusion</h3><div>This first integrative <em>in silico-invitro</em> analysis of dual herbal <em>Rasayana</em> extracts reveals multi-target anticancer activity and validates the stability of the key protein-ligand interactions suggesting synergistic therapeutic potential of <em>W. somnifera</em> with <em>P. emblica</em> as promising lead formulations for further drug development.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"17 4","pages":"Article 101373"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148578817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}