Journal of Ayurveda and Integrative Medicine最新文献

{"title":"Effect of Anjana Kriyakalpa in refractive errors: Evaluation through refraction and corneal topography indices - A case series","authors":"Deenadayal Devarajan,&nbsp;Manjusha Rajagopala","doi":"10.1016/j.jaim.2025.101128","DOIUrl":"10.1016/j.jaim.2025.101128","url":null,"abstract":"<div><div>Refractive errors, a significant cause of visual impairment, is becoming increasingly prevalent in India, particularly among school children. This case series describes the effects of <em>Pathyadi Varti Anjana</em>, an Ayurvedic herbo-mineral formulation, in refractive errors associated with distant vision. Three patients with varying degrees of myopia and myopic astigmatism were treated with <em>Pathyadi Varti</em> applied as <em>Anjana,</em> twice daily for a period of three to six weeks. Parameters including Visual Acuity (VA), Objective Refraction (OR), Clinical Refraction (CR), and corneal topography metrics such as Simulated Keratometry (Sim K's), Shape Factor (SF), Corneal Irregularity Measurement (CIM), Toric Keratometric Mean (TKM), Root Mean Square (RMS), and PathFinder II Normalcy (PathFII) were assessed before and after treatment. The results demonstrated improvements in unaided VA, reductions in spherical and cylindrical refractive errors, and enhancements in corneal surface regularity and wavefront aberrations across all cases. These findings suggest that <em>Pathyadi Varti Anjana</em> may offer a promising Ayurvedic approach to the management of refractive errors, supporting its potential role in contemporary ophthalmic practices.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 3","pages":"Article 101128"},"PeriodicalIF":1.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143740065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of one week of Ayurveda-based therapy, lifestyle and diet modification in heart failure patients with reduced ejection fraction: A retrospective study","authors":"Rohit Sane ,&nbsp;Rahul Mandole ,&nbsp;Gurudatta Amin ,&nbsp;Tejashree Jadhav ,&nbsp;Prafull Yashwantrao ,&nbsp;Snehal Dongre ,&nbsp;Swapnil Srivastav","doi":"10.1016/j.jaim.2024.101083","DOIUrl":"10.1016/j.jaim.2024.101083","url":null,"abstract":"<div><h3>Background</h3><div>Management of Chronic Heart Failure (CHF) is quite challenging, and its prognosis remains poor. Thus, alternative and conventional treatment approaches with lifestyle modifications are needed to enhance the quality of life. One such approach is heart failure reversal treatment (HFRT) that includes Snehana (massage), Swedana (fomentation therapy), Basti (per rectal drug administration), and Hridaydhara.</div></div><div><h3>Objective</h3><div>The present study evaluated the effect of HFRT in CHF patients with heart failure with reduced ejection fraction (HFrEF), and relate the clinical benefit and change in hemodynamic parameters intending to improve the quality of life.</div></div><div><h3>Methods</h3><div>This study evaluated data from known CHF patients who visited the Madhavbaug Hospital between May 2021 to October 2021. Patients of either gender with NYHA Class I, II, III and LVEF&lt;40 who completed 7-day (12 sessions, one each at day 1 and 7 and remaining days 2 sessions/day) HFRT therapy were included in the study. Data on blood pressure, abdominal girth (ABG) and cardiac parameters [End diastolic volume (EDV), Ejection factor (EF), Systemic vascular resistance (SVR), 6-min walk test (6 MW T), etc. was analysed pre and post-HFRT.</div></div><div><h3>Results</h3><div>85 CHF patients with a mean age of 57 years and 67 (78.8%) males were enrolled in the study. The calculated EF, 6 MW T, stroke volume, and SVR significantly changed (p &lt; 0.05) on Day 7 post-HFRT intervention. Though, BMI, ABG, EDV, SV and CO showed a positive change, it was not statistically significant.</div></div><div><h3>Conclusion</h3><div>HFRT demonstrated a significant change in the hemodynamics, which was also impacted positively by the improvement in 6 MW T, thereby enhancing the quality of life.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 2","pages":"Article 101083"},"PeriodicalIF":1.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of meditation on sustained attention in nonclinical population: An extensive review","authors":"Abinash Roy,&nbsp;Pailoor Subramanya","doi":"10.1016/j.jaim.2024.101057","DOIUrl":"10.1016/j.jaim.2024.101057","url":null,"abstract":"<div><h3>Background</h3><div>Meditation, encompassing focussed attention (FA) and open monitoring (OM) approaches, is recognised for its potential to enhance cognitive functions. Sustained attention, a critical component of attentional processes, influences cognitive capacity and is linked to meditation benefits. However, a robust and extensive review analysis needs to address the specific relationship between meditation and sustained attention in nonclinical populations.</div></div><div><h3>Methods</h3><div>Following PRISMA guidelines, the authors reviewed English articles published from 2013 to 2023 in Q1 SCOPUS-indexed journals. Inclusion criteria comprised original research studies exploring the impact of meditation on sustained attention in healthy individuals. The modified Jadad Scale assessed methodological quality.</div></div><div><h3>Results</h3><div>12 studies (four RCTs, eight non-RCTs) with 1447 participants were included. Concentrative or FA meditation demonstrated consistent positive effects on sustained attention, including reduced perceived stress and increased focussed attention. OM meditation significantly improved sustained attention, as evidenced by reduced mind wandering and enhanced N2 responses. Meditators consistently outperformed non-meditators in sustained attention tasks, demonstrating faster reactions and lower error rates.</div></div><div><h3>Discussion</h3><div>This review explored the impact of meditation on sustained attention across diverse non-clinical populations through 12 investigations involving 1447 subjects with meditation interventions spanning from 21 days to 3 months. The study revealed that both FA and OM meditation approaches positively impact sustained attention, highlighting their potential role in enhancing cognitive function. Meditators consistently exhibited superior sustained attention abilities, suggesting the cognitive benefits of regular meditation practice. The findings of this study are consistent with prior research, contributing to the growing body of knowledge on the advantageous impacts of meditation on sustained attention. However, caution is needed in generalizing findings due to study limitations. Future research should use standardized methodologies and conduct longer-term follow-ups to better elucidate the effects of meditation interventions on sustained attention across diverse populations.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 2","pages":"Article 101057"},"PeriodicalIF":1.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro anti-inflammatory and in silico anti-viral assessment of phytoconstituents in polyherbal Ayurvedic formulation ‘Arogyamrita Kwath’","authors":"Akash Dey ,&nbsp;Akash G. Kendre ,&nbsp;Madhu babu Dande ,&nbsp;Yogita B. Tandalekar ,&nbsp;Shreyanshi Kulshreshtha ,&nbsp;Amit Srivastava ,&nbsp;Alok Goyal ,&nbsp;Ramadas Maganti ,&nbsp;Sumit Srivastava ,&nbsp;Prasad V. Bharatam ,&nbsp;Sanjay M. Jachak","doi":"10.1016/j.jaim.2024.101076","DOIUrl":"10.1016/j.jaim.2024.101076","url":null,"abstract":"<div><h3>Background</h3><div>Arogyamrita Kwath (AMK) is a polyherbal decoction comprising ten medicinal plants, <em>viz</em>., <em>Albizia lebbeck</em>, <em>Andrographis paniculata</em>, <em>Tinospora cordifolia</em>, <em>Adhatoda vasica</em>, <em>Solanum xanthocarpum</em>, <em>Curcuma longa</em>, <em>Glycyrrhiza glabra</em>, <em>Terminalia bellirica</em>, <em>Withania somnifera</em> and <em>Trachyspermum ammi</em>. The plants of the AMK formulation are traditionally used for the treatment of inflammation and respiratory ailments, but no scientific evidence has been reported so far for this formulation.</div></div><div><h3>Objectives</h3><div>To evaluate anti-inflammatory activity of AMK formulation <em>in vitro</em> and its fractions and to predict <em>in silico</em> anti-viral activity of identified potential phytoconstituents.</div></div><div><h3>Materials and methods</h3><div>The MTT cell cytotoxicity assay, nitric oxide (NO) inhibition assay and cytokines assay were carried out at concentrations 100 and 200 μg/mL. The phytoconstituents were identified by UPLC-PDA and UPLC-HRMS analyses. For pharmacoinformatics study molecular docking and molecular dynamics methods were used.</div></div><div><h3>Results</h3><div>The study revealed that AMK significantly inhibited NO in comparison to dexamethasone (100 μg/mL) and pro-inflammatory cytokines in RAW264.7 cells. The three fractions, <em>n</em>-hexane, EtOAc and <em>n</em>-BuOH prepared from the AMK formulation were non-cytotoxic against RAW264.7 murine macrophage cells during MTT cytotoxicity assay and showed satisfactory results during cytokines assay. Ethyl acetate fraction contains active phytoconstituents in appreciable quantities. 16 phytoconstituents have been identified by UPLC-HRMS analysis in the formulation and four phytocompounds were quantified by UPLC-PDA. Molecular dynamics study helped in identifying two macromolecular targets (viral replicase and the membrane protein), which are relatively more important.</div></div><div><h3>Conclusion</h3><div>In the present study, anti-inflammatory activity of AMK was evaluated and the claimed anti-viral property was re-confirmed by molecular modelling in this work. The results clearly established that AMK showed remarkable anti-inflammatory and anti-viral activities.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 2","pages":"Article 101076"},"PeriodicalIF":1.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143621243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Vineeta V. Deshmukh","doi":"10.1016/j.jaim.2024.101090","DOIUrl":"10.1016/j.jaim.2024.101090","url":null,"abstract":"","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 2","pages":"Article 101090"},"PeriodicalIF":1.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Randomized double blind placebo controlled multicentric clinical trial of Ayush M-3 in the management of migraine- A study protocol","authors":"Shweta Chaudhary ,&nbsp;Shruti Khanduri ,&nbsp;Sunita Mata ,&nbsp;B.C.S. Rao ,&nbsp;Kuljeet Singh Anand ,&nbsp;R. Kishore Kumar ,&nbsp;K.G. Sudha Kumari ,&nbsp;Bidhan Mahajan ,&nbsp;Rakesh Kumar Rana ,&nbsp;Richa Singhal ,&nbsp;Shweta Mata ,&nbsp;Renu Makhija ,&nbsp;Bharti ,&nbsp;N. Srikanth ,&nbsp;K.S. Dhiman ,&nbsp;Rabinarayan Acharya","doi":"10.1016/j.jaim.2024.101054","DOIUrl":"10.1016/j.jaim.2024.101054","url":null,"abstract":"<div><h3>Background</h3><div>Migraine is acknowledged as an intricate and incapacitating neurological malady, profoundly exerting a potent influence on the health-related quality of life of patients causing disability, and augmented burden on families, and entire communities. Notwithstanding the strides made in the field of headache disorder management, there remains a cohort of migraine-afflicted patients who do not encounter satisfactory alleviation. This emphasizes the need for alternative systems in addressing the issue and there is a growing interest in exploring Indian system of medicine to address migraine symptoms. <strong>Objective:</strong> Due to the dearth of prospective, high-quality randomized controlled trials, this protocol aims to conduct a scientific study of the polyherbal formulation ‘Ayush M3’ to assess its safety and efficacy in patients suffering with migraine.</div></div><div><h3>Materials and Methods</h3><div>A prospective, randomized, double blind, parallel-group, placebo controlled, multi – centric, two-arm, exploratory study has been designed to enrol a total of 360 patients at three sites. Migraine patients with or without aura diagnosed as per International Headache Society-ICHD-III below Grade III of MIDAS will be randomized in a 1:1 ratio and administered the trial drug, Ayush M − 3 or placebo for twice a day after food for 16 weeks.</div></div><div><h3>Outcome measures</h3><div>The primary outcomes measures for the study will be changes in the character of disease in terms of frequency, intensity of headache as per VAS (0–10) and duration of episode of headache. Secondary outcome measure will be changes in MIDAS, MSQ2.1, changes in associated symptoms of migraine, change in the use of analgesics/symptom alleviating medication &amp; Change in the required dose/withdrawal of Anti-hypertensive drugs if any. Assessment will be conducted on an interval of 28 days till 16 weeks and an intervention free follow up at the end of week 20.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 2","pages":"Article 101054"},"PeriodicalIF":1.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroencephalogram analysis on alpha/beta and theta/beta ratios due to shirodhara","authors":"Yogeshwar Dasari ,&nbsp;Lakshmana Rao Chebolu ,&nbsp;Venkatesh Balasubramanian","doi":"10.1016/j.jaim.2024.101094","DOIUrl":"10.1016/j.jaim.2024.101094","url":null,"abstract":"<div><h3>Background</h3><div><em>Shirodhara</em> is a brain relaxation therapy. The treatment induces a relaxed state with awareness that results in treating neurological conditions like mental stress (depression/anxiety/hypertension) and some of its beneficial side effects are soothing the central nervous system.</div></div><div><h3>Objectives</h3><div>In the present study, the investigation aimed to analyze the ratios of brain waves, particularly focusing on the frontal region.</div></div><div><h3>Materials and Methods</h3><div>In this study, <em>Shirodhara</em> with “<em>Ksheerabala thailam</em>” medicinal oil was conducted on 16 participants and pre-post treatment brain signals were collected using an EEG acquisition tool and it was found that treatment induced relaxation aspects were observed among participants.</div></div><div><h3>Results and Conclusion</h3><div>Welch's Fast Fourier transform (FFT) was used to analyze the EEG signals in order to obtain power spectral density (PSD) features, which indicated a signal's power dispersed across a range of frequencies. The effectiveness of treatment has been evaluated by examining the spectral power ratios of alpha/beta, theta/beta in the frontal region of the brain and physiological and psychological parameters.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 2","pages":"Article 101094"},"PeriodicalIF":1.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143637663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ayurveda management of post TURP urethral stricture: A case report","authors":"T. Sushendra ,&nbsp;Foram P. Joshi ,&nbsp;Hetal L. Nakrani ,&nbsp;Manisha Kapadiya ,&nbsp;Saurabh P. Patel","doi":"10.1016/j.jaim.2024.101084","DOIUrl":"10.1016/j.jaim.2024.101084","url":null,"abstract":"<div><div>Post TURP (Trans Urethral Resection of Prostate) Urethral stricture incident rates have been reported between 2.2 and 9.8%. Weak urine stream, recurrent urethral infections, dysuria, retention of urine, etc. are the chief presenting complaints of the clinical cases. Sushruta described 12 types of <em>Mootraghata</em> (∼clinical conditions stating obstructive uropathies). Lower Urinary Tract Symptoms (LUTS) have been provided with separate diagnostic notation with its description in <em>Mootraghata</em>. Sushruta narrated <em>Mootrotsanga</em>-a clinical condition confirming obstruction of urine in the lower urinary tract developing LUTS. Sushruta recommended <em>Uttarabasti</em> (intra-urethral douching) as a line of treatment to get rid of obstructive uropathies of various origins. This case is about a 70-year-old nondiabetic, nonhypertensive male who reported a 2-month history of weak urinary stream, burning micturition, and increased micturition frequency. The patient was diagnosed with stricture at the distal penile urethra near the external urethral meatus and stricture at the junction of the middle and posterior urethra. This case was managed by, <em>Sadhyo Virechana</em> (Therapeutic purgation)<em>,</em> followed by <em>Uttarbasti</em> (intra-urethral douche) procedure with <em>Jatyadi</em> oil and other adjuvent Ayurveda medicaments like <em>Eranda Bhrishta Haritaki, Gokshuradi Guggul</em> and <em>Varunadi Kwatha</em> for 21 days. Encouraging outcome was achieved in terms of complete reversal from Urethral stricture and evident with Ascending Urethrogram (AUG) report. Since the last 1 years' follow-up, the patient has been living symptom-free. LUTS and especially, stricture pathologies can be managed through safe and noninvasive procedures like <em>Uttarabasti</em>.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 2","pages":"Article 101084"},"PeriodicalIF":1.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143637662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of self-acupressure on quality of life and abdominal pain severity among patients with inflammatory bowel disease: A randomized sham-controlled trial","authors":"Nahid Rajai ,&nbsp;Zahra Abbasi ,&nbsp;Amir Hosein Pishgooie ,&nbsp;Fatemeh Teymouri ,&nbsp;Mohammad Imanipour","doi":"10.1016/j.jaim.2024.101080","DOIUrl":"10.1016/j.jaim.2024.101080","url":null,"abstract":"<div><h3>Background</h3><div>Acupressure is being used by patients to relieve functional gastrointestinal disorders.</div></div><div><h3>Objective</h3><div>The impact of acupressure on quality of life (QoL) and pain has been shown in patients with both acute and chronic conditions. However, its effects on individuals with inflammatory bowel disease (IBD) remain uncertain. This trial aims to explore the potential effects of self-acupressure on QoL and abdominal pain severity in IBD patients.</div></div><div><h3>Methods</h3><div>This randomized sham-controlled trial involved 56 outpatients with IBD referred to the emergency department, who were randomly assigned to either an acupressure group or a sham group. All patients received standard care along with daily follow-up calls. Those in the acupressure group applied pressure at four specific points, while the sham group engaged in superficial self-touch at corresponding points. Both interventions occurred three times daily for four weeks. The quality of life was assessed using the IBDQ-9 questionnaire and a visual analog scale (VAS) at both baseline and endpoint.</div></div><div><h3>Results</h3><div>The mean IBDQ-9 score was significantly higher in the acupressure group compared to the sham group at the trial's end (P &lt; 0.001). Likewise, the mean VAS score was notably lower in the acupressure group at the endpoint (P &lt; 0.001). Additionally, the change in mean IBDQ-9 scores from baseline to endpoint favored the acupressure group significantly (P &lt; 0.001), and the same significant difference was observed for the mean VAS score change (P &lt; 0.001).</div></div><div><h3>Conclusion</h3><div>Daily follow-up-guided self-acupressure may improve quality of life and alleviate abdominal pain in IBD sufferers.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 2","pages":"Article 101080"},"PeriodicalIF":1.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143696789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting cell cycle arrest in breast cancer by phytochemicals from Caryto urens L. fruit ethyl acetate fraction: in silico and in vitro validation","authors":"Ghanshyam Parmar,&nbsp;Jay Mukesh Chudasama,&nbsp;Ashish Shah,&nbsp;Chintan Aundhia,&nbsp;Sunil Kardani","doi":"10.1016/j.jaim.2024.101095","DOIUrl":"10.1016/j.jaim.2024.101095","url":null,"abstract":"<div><h3>Background</h3><div><em>Caryota urens</em>, also known as Shivjata, has been documented in ancient Indian texts for its therapeutic benefits, addressing conditions from seminal weakness to gastric ulcers. This study aims to investigate its contemporary medicinal potential in treating breast cancer.</div></div><div><h3>Objectives</h3><div>The study focuses on exploring the therapeutic potential of <em>Caryota urens</em> fruit against breast cancer, specifically targeting cell cycle genes CDK1, CDC25A, and PLK1 through bioinformatics, network pharmacology, and in vitro validation.</div></div><div><h3>Materials and methods</h3><div>Using mass spectrometry and nuclear magnetic resonance (NMR), 60 key phytoconstituents from <em>Caryota urens</em> fruit were identified. Bioinformatics analysis, integrating Gene Cards and GEO databases, 15,474 breast cancer-associated genes focusing on the HR+/HER2-subtype were identified. Molecular docking and qPCR validated the interactions of key phytoconstituents, particularly Episesamin, with CDK1, CDC25A, and PLK1. In vitro studies were conducted on the MCF7 cell line, supplemented by ROC and survival analyses to evaluate diagnostic and therapeutic potential.</div></div><div><h3>Results</h3><div>The bioinformatics analysis identified CDK1, CDC25A, and PLK1 as pivotal genes regulating cell cycle progression and breast cancer tumorigenesis. Network pharmacology and in vitro studies indicated that phytoconstituents, especially Episesamin, downregulated these genes in breast cancer cells. Molecular docking and qPCR confirmed these interactions, and ROC and survival analyses underscored their diagnostic and therapeutic significance.</div></div><div><h3>Conclusions</h3><div>This study suggests that <em>Caryota urens</em> fruit extract, particularly Episesamin, may inhibit breast cancer metastasis by downregulating CDK1, CDC25A, and PLK1, offering promising new strategies for targeting the cell cycle in breast cancer and emphasizing the value of integrating bioinformatics with experimental methods in cancer research.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 2","pages":"Article 101095"},"PeriodicalIF":1.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143600897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}