Journal of biochemistry and molecular biology最新文献_第7页

Antioxidant and anti-hyperglycemic activity of polysaccharide isolated from Dendrobium chrysotoxum Lindl. 黄曲石斛多糖的抗氧化和抗高血糖活性研究。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.670

Yaping Zhao, Young-Ok Son, So-Soon Kim, Yong-Suk Jang, Jeong-Chae Lee

{"title":"Antioxidant and anti-hyperglycemic activity of polysaccharide isolated from Dendrobium chrysotoxum Lindl.","authors":"Yaping Zhao, Young-Ok Son, So-Soon Kim, Yong-Suk Jang, Jeong-Chae Lee","doi":"10.5483/bmbrep.2007.40.5.670","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.5.670","url":null,"abstract":"Although polysaccharide is believed to play an important role in the medicinal effect of Dendrobium chrysotoxum Lindl (DCL), its role as an antioxidant and in anti-hyperglycemic induction was not reported. In this study, polysaccharide with molecular weight of approximately 150 kDa, herein named DCLP, was isolated from the stem of DCL, and its antioxidative, hypoglycemic and immune stimulating effects were evaluated using various in vitro and in vivo assay systems. DCLP inhibited hydroxyl radicals (*OH)-mediated deoxyribose degradation by scavenging hydroxyl radicals directly as well as by chelating iron ions. DCLP also showed dose-dependent scavenging activity on superoxide anions (O2*) and offered significant protection (p < 0.001) against glucose oxidasemediated cytotoxicity in Jurkat cells. DCLP had immune stimulating effects, as evidenced by the DCLP-mediated increases in the level of DNA synthesis, viability, and cytokine secretion in mouse lymphocytes. Moreover, oral administration of DCLP produced a significant reduction in blood glucose level in alloxan-induced diabetic mice. These findings suggest that DCLP has a potential utility in treating patients who require enhanced antioxidation, immune function and/or hypoglycemic activity.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 5","pages":"670-7"},"PeriodicalIF":0.0,"publicationDate":"2007-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27041451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 96

Identification of differentially expressed genes related to intramuscular fat development in the early and late fattening stages of hanwoo steers. 汉宇阉牛育肥早期和后期肌内脂肪发育相关差异表达基因的鉴定。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.757

Seung-Hwan Lee, Eung-Woo Park, Yong-Min Cho, Sung-Kon Kim, Jun-Heon Lee, Jin-Tae Jeon, Chang-Soo Lee, Seok-Ki Im, Sung-Jong Oh, J M Thompson, Duhak Yoon

{"title":"Identification of differentially expressed genes related to intramuscular fat development in the early and late fattening stages of hanwoo steers.","authors":"Seung-Hwan Lee, Eung-Woo Park, Yong-Min Cho, Sung-Kon Kim, Jun-Heon Lee, Jin-Tae Jeon, Chang-Soo Lee, Seok-Ki Im, Sung-Jong Oh, J M Thompson, Duhak Yoon","doi":"10.5483/bmbrep.2007.40.5.757","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.5.757","url":null,"abstract":"Marbling of cattle meat is dependent on the coordinated expression of multiple genes. Cattle dramatically increase their intramuscular fat content in the longissimus dorsi muscle between 12 and 27 months of age. We used the annealing control primer (ACP)-differential display RT-PCR method to identify differentially expressed genes (DEGs) that may participate in the development of intramuscular fat between early (12 months old) and late fattening stages (27 months old). Using 20 arbitrary ACP primers, we identified and sequenced 14 DEGs. BLAST searches revealed that expression of the MDH, PI4-K, ferritin, ICER, NID-2, WDNMI, telethonin, filamin, and desmin (DES) genes increased while that of GAPD, COP VII, ACTA1, CamK II, and nebulin decreased during the late fattening stage. The results of functional categorization using the Gene Ontology database for 14 known genes indicated that MDH, GAPD, and COP VII are involved in metabolic pathways such as glycolysis and the TCA cycle, whereas telethonin, filamin, nebulin, desmin, and ACTA1 contribute to the muscle contractile apparatus, and PI4-K, CamK II, and ICER have roles in signal transduction pathways regulated by growth factor or hormones. The final three genes, NID-2, WDNMI, and ferritin, are involved in iron transport and extracellular protein inhibition. The expression patterns were confirmed for seven genes (MDH, PI4-K, ferritin, ICER, nebulin, WDNMI, and telethonin) using real-time PCR. We found that the novel transcription repressor ICER gene was highly expressed in the late fattening stage and during bovine preadipocyte differentiation. This information may be helpful in selecting candidate genes that participate in intramuscular fat development in cattle.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 5","pages":"757-64"},"PeriodicalIF":0.0,"publicationDate":"2007-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27041841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 64

Binding characteristics to mosquito-larval midgut proteins of the cloned domain II-III fragment from the Bacillus thuringiensis Cry4Ba toxin. 苏云金芽孢杆菌Cry4Ba毒素克隆结构域II-III片段与蚊幼虫中肠蛋白的结合特性

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.783

Seangdeun Moonsom, Urai Chaisri, Watchara Kasinrerk, Chanan Angsuthanasombat

{"title":"Binding characteristics to mosquito-larval midgut proteins of the cloned domain II-III fragment from the Bacillus thuringiensis Cry4Ba toxin.","authors":"Seangdeun Moonsom, Urai Chaisri, Watchara Kasinrerk, Chanan Angsuthanasombat","doi":"10.5483/bmbrep.2007.40.5.783","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.5.783","url":null,"abstract":"Receptor binding plays an important role in determining host specificity of the Bacillus thuringiensis Cry delta-endotoxins. Mutations in domains II and III have suggested the participation of certain residues in receptor recognition and insect specificity. In the present study, we expressed the cloned domain II-III fragment of Cry4Ba and examined its binding characteristics to mosquito-larval midgut proteins. The 43-kDa Cry4Ba-domain II-III protein over-expressed in Escherichia coli as inclusion bodies was only soluble when carbonate buffer, pH 10.0 was supplemented with 4 M urea. After renaturation via stepwise dialysis and subsequent purification, the refolded domain II-III protein, which specifically reacts with anti Cry4Ba-domain III monoclonal antibody, predominantly exists as a beta-sheet structure determined by circular dichroism spectroscopy. In vitro binding analysis to both histological midgut tissue sections and brush border membrane proteins prepared from susceptible Aedes aegypti mosquito-larvae revealed that the isolated Cry4Ba-domain II-III protein showed binding functionality comparable to the 65-kDa full-length active toxin. Altogether, the data present the 43-kDa Cry4Ba fragment comprising domains II and III that was produced in isolation was able to retain its receptor-binding characteristics to the target larval midgut proteins.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 5","pages":"783-90"},"PeriodicalIF":0.0,"publicationDate":"2007-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27041844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Preparation and Characterization of Paclitaxel-loaded PLGA nanoparticles coated with cationic SM5-1 single-chain antibody. 阳离子SM5-1单链抗体包被紫杉醇负载PLGA纳米颗粒的制备与表征。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.731

Geng Kou, Jie Gao, Hao Wang, Huaiwen Chen, Bohua Li, Dapeng Zhang, Shuhui Wang, Sheng Hou, Weizhu Qian, Jianxin Dai, Yanqiang Zhong, Yajun Guo

{"title":"Preparation and Characterization of Paclitaxel-loaded PLGA nanoparticles coated with cationic SM5-1 single-chain antibody.","authors":"Geng Kou, Jie Gao, Hao Wang, Huaiwen Chen, Bohua Li, Dapeng Zhang, Shuhui Wang, Sheng Hou, Weizhu Qian, Jianxin Dai, Yanqiang Zhong, Yajun Guo","doi":"10.5483/bmbrep.2007.40.5.731","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.5.731","url":null,"abstract":"The purpose of this study was to develop paclitaxel-loaded poly(lactide-co-glycolide) (PLGA) nanoparticles coated with cationic SM5-1 single-chain antibody (scFv) containing a polylysine (SMFv-polylys). SM5-1 scFv (SMFv) is derived from SM5-1 monoclonal antibody, which binds to a 230 kDa membrane protein specifically expressed on melanoma, hepatocellular carcinoma and breast cancer cells. SMFv-polylys was expressed in Escherichia coli and purified by cation-exchange chromatography. Purified SMFv-polylys was fixed to paclitaxel-loaded PLGA nanoparticles to form paclitaxel-loaded PLGA nanoparticles coated with SMFv-polylys (Ptx-NP-S). Ptx-NP-S was shown to retain the specific antigen-binding affinity of SMFv-polylys to SM5-1 binding protein-positive Ch-hep-3 cells. Finally, the cytotoxicity of Ptx-NP-S was evaluated by a non-radioactive cell proliferation assay. It was demonstrated that Ptx-NP-S had significantly enhanced in vitro cytotoxicity against Ch-hep-3 cells as compared with non-targeted paclitaxel-loaded PLGA nanoparticles. In conclusion, our results suggest that cationic SMFv-polylys has been successfully generated and may be used as targeted ligand for preparing cancer-targeted nanoparticles.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 5","pages":"731-9"},"PeriodicalIF":0.0,"publicationDate":"2007-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27040302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 47

Cotton GhKCH2, a plant-specific kinesin, is low-affinitive and nucleotide-independent as binding to microtubule. 棉花GhKCH2是一种植物特异性的激酶，与微管的结合是低亲和的，不依赖于核苷酸。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.723

Tao Xu, Xuewei Sun, Shiling Jiang, Dongtao Ren, Guoqin Liu

{"title":"Cotton GhKCH2, a plant-specific kinesin, is low-affinitive and nucleotide-independent as binding to microtubule.","authors":"Tao Xu, Xuewei Sun, Shiling Jiang, Dongtao Ren, Guoqin Liu","doi":"10.5483/bmbrep.2007.40.5.723","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.5.723","url":null,"abstract":"Kinesin is an ATP-driven microtubule motor protein that plays important roles in control of microtubule dynamics, intracellular transport, cell division and signal transduction. The kinesin superfamily is composed of numerous members that are classified into 14 subfamilies. Animal kinesins have been well characterized. In contrast, plant kinesins have not yet to be characterized adequately. Here, a novel plant-specific kinesin gene, GhKCH2, has been cloned from cotton (Gossypium hirsutum) fibers and biochemically identified by prokaryotic expression, affinity purification, ATPase activity assay and microtubule-binding analysis. The putative motor domain of GhKCH2, M396-734 corresponding to amino acids Q396-N734 was fused with 6xHis-tag, soluble-expressed in E. coli and affinity-purified in a large amount. The biochemical analysis demonstrated that the basal ATPase activity of M396-734 is not activated by Ca2+, but stimulated 30-fold max by microtubules. The enzymatic activation is microtubule-concentration-dependent, and the concentration of microtubules that corresponds to half-maximum activation was about 11 microM, much higher than that of other kinesins reported. The cosedimentation assay indicated that M396-734 could bind to microtubules in vitro whenever the nucleotide AMP-PNP is present or absent. As a plant-specific microtubule-dependent kinesin with a lower microtubule-affinity and a nucleotide-independent microtubule-binding ability, cotton GhKCH2 might be involved in the function of microtubules during the deposition of cellulose microfibrils in fibers or the formation of cell wall.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 5","pages":"723-30"},"PeriodicalIF":0.0,"publicationDate":"2007-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27040301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Phosphoproteomic analysis of AML14.3D10 cell line as a model system of eosinophilia. AML14.3D10细胞系嗜酸性粒细胞模型系统的磷酸化蛋白质组学分析。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.765

Su In Ryu, Won Kon Kim, Hyun Ju Cho, Phil Young Lee, Hyeyun Jung, Tae-Sung Yoon, Jeong Hee Moon, Sunghyun Kang, Haryoung Poo, Kwang-Hee Bae, Sang Chul Lee

{"title":"Phosphoproteomic analysis of AML14.3D10 cell line as a model system of eosinophilia.","authors":"Su In Ryu, Won Kon Kim, Hyun Ju Cho, Phil Young Lee, Hyeyun Jung, Tae-Sung Yoon, Jeong Hee Moon, Sunghyun Kang, Haryoung Poo, Kwang-Hee Bae, Sang Chul Lee","doi":"10.5483/bmbrep.2007.40.5.765","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.5.765","url":null,"abstract":"Eosinophils act as effectors in the inflammatory reactions of allergic diseases including atopic dermatitis. Atopic dermatitis patients and others with allergic disorders suffer from eosinophilia, an accumulation of eosinophils due to increased survival or decreased apoptosis of eosinophils. In this study, a differential phosphoproteome analysis of AML14.3D10 eosinophil cell line after treatment with IL-5 or dexamethasone was conducted in an effort to identify the phosphoproteins involved in the proliferation or apoptosis of eosinophils. Proteins were separated by 2-DE and alterations in phosphoproteins were then detected by Pro-Q Diamond staining. The significant quantitative changes were shown in nineteen phosphoproteins including retinoblastoma binding protein 7, MTHSP75, and lymphocyte cytosolic protein 1. In addition, seven phosphoproteins including galactokinase I, and proapolipoprotein, were appeared after treatment with IL-5 or dexamethasone. Especially, the phospho-APOE protein was down-regulated in IL-5 treated AML14.3D10, while the more heavily phosphorylated APOE form was induced after dexamethasone treatment. These phosphoproteome data for the AML14.3D10 cell line may provide clues to understand the mechanism of eosinophilia as well as allergic disorders including atopic dermatitis.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 5","pages":"765-72"},"PeriodicalIF":0.0,"publicationDate":"2007-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27041842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

The replicon initiation burst released by reoxygenation of hypoxic T24 cells is accompanied by changes of MCM2 and Cdc7. 缺氧T24细胞再氧化释放的复制子起始爆发伴随着MCM2和Cdc7的改变。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.805

Leenus Martin

{"title":"The replicon initiation burst released by reoxygenation of hypoxic T24 cells is accompanied by changes of MCM2 and Cdc7.","authors":"Leenus Martin","doi":"10.5483/bmbrep.2007.40.5.805","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.5.805","url":null,"abstract":"Although MCM2 is obviously important for the initiation of eukaryotic DNA replication, its role in O2 dependent regulation of replicon initiation is poorly understood. In this report, I analysed the changes of MCM2 during the transition from hypoxically suppressed replicon initiation to the burst of initiation triggered by reoxygenation in T24 cells. A high level of chromatin bound and nucleosolic MCM2 was found under the hypoxic replicon arrest. In contrast low cytosolic MCM2 was noticed. Recovery of O2 induced phosphorylation and diminution of chromatin bound MCM2, whereas cytosolic MCM2 increased. The level of chromatin bound Cdc7 did not change significantly upon reoxygenation. However, after reoxygenation, significant phosphorylation of Cdc7 and an increase of coimmunoprecipitation with its substrate (MCM2) were observed. This provides a hint that reoxygenation may promote the kinase activity of Cdc7. These changes might be the critical factors in O2 dependent regulation of replicon initiation. Moreover, phosphorylation of Cdc7 by Cdk2 can be observed in vitro, but seems to fail to regulate the level of chromatin bound Cdc7 as well as the changes of MCM2 in response to reoxygenation of hypoxically suppressed cells.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 5","pages":"805-13"},"PeriodicalIF":0.0,"publicationDate":"2007-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27041847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Photoinhibition induced alterations in energy transfer process in phycobilisomes of PS II in the cyanobacterium, Spirulina platensis. 光抑制诱导蓝藻PS II藻胆体能量传递过程的改变。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.644

Duvvuri Prasanna Kumar, Sistla D S Murthy

{"title":"Photoinhibition induced alterations in energy transfer process in phycobilisomes of PS II in the cyanobacterium, Spirulina platensis.","authors":"Duvvuri Prasanna Kumar, Sistla D S Murthy","doi":"10.5483/bmbrep.2007.40.5.644","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.5.644","url":null,"abstract":"Exposure of algae or plants to irradiance from above the light saturation point of photosynthesis is known as high light stress. This high light stress induces various responses including photoinhibition of the photosynthetic apparatus. The degree of photoinhibition could be clearly determined by measuring the parameters such as absorption and fluorescence of chromoproteins. In cyanobacteria and red algae, most of the photosystem (PS) II associated light harvesting is performed by a membrane attached complex called the phycobilisome (PBS). The effects of high intensity light (1000-4000 micromol photons m(-2) s(-1)) on excitation energy transfer from PBSs to PS II in a cyanobacterium Spirulina platensis were studied by measuring room temperature PC fluorescence emission spectra. High light (3000 micromol photons m(-2) s(-1)) stress had a significant effect on PC fluorescence emission spectra. On the other hand, light stress induced an increase in the ratio of PC fluorescence intensity of PBS indicating that light stress inhibits excitation energy transfer from PBS to PS II. The high light treatment to 3000 micromol photons m(-2) s(-1) caused disappearance of 31.5 kDa linker polypeptide which is known to link PC discs together. In addition we observed the similar decrease in the other polypeptide contents. Our data concludes that the Spirulina cells upon light treatment causes alterations in the phycobiliproteins (PBPs) and affects the energy transfer process within the PBSs.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 5","pages":"644-8"},"PeriodicalIF":0.0,"publicationDate":"2007-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27041447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Gene regulations in HBV-related liver cirrhosis closely correlate with disease severity. hbv相关肝硬化的基因调控与疾病严重程度密切相关。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.814

Seram Lee, Soyoun Kim

{"title":"Gene regulations in HBV-related liver cirrhosis closely correlate with disease severity.","authors":"Seram Lee, Soyoun Kim","doi":"10.5483/bmbrep.2007.40.5.814","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.5.814","url":null,"abstract":"Liver cirrhosis (LC) is defined as comprising diffuse fibrosis and regenerating nodules of the liver. The biochemical and anatomical dysfunction in LC results from both reduced liver cell number and portal vascular derangement. Although several studies have investigated dysregulated genes in cirrhotic nodules, little is known about the genes implicated in the pathophysiologic change of LC or about their relationship with the degree of decompensation. Here, we applied cDNA microarray analysis using 38 HBsAg-positive LC specimens to identify the genes dysregulated in HBV-associated LC and to evaluate their relation to disease severity. Among 1063 known cancer- and apoptosis-related genes, we identified 104 genes that were significantly up- (44) or down- (60) regulated in LC. Interestingly, this subset of 104 genes was characteristically correlated with the degree of decompensation, called the Pugh-Child classification (20 Pugh-Child A, 10 Pugh-Child B, and 8 Pugh-Child C). Patient samples from Pugh-Child C exhibited a distinct pattern of gene expression relative to those of Pugh-Child A and B. Especially in Pugh-Child C, genes encoding hepatic proteins and metabolizing enzymes were significantly down-regulated, while genes encoding various molecules related to cell replication were up-regulated. Our results suggest that subsets of genes in liver cells correspond to the pathophysiologic change of LC according to disease severity and possibly to hepatocarcinogenesis.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 5","pages":"814-24"},"PeriodicalIF":0.0,"publicationDate":"2007-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27041848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

A novel anticoagulant protein with high affinity to blood coagulation factor Va from Tegillarca granosa. 一种与凝血因子Va高亲和力的新型抗凝蛋白。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.832

Won-Kyo Jung, Hee-Yeon Jo, Zhong-Ji Qian, Young-Ju Jeong, Sae-Gwang Park, Il-Whan Choi, Se-Kwon Kim

{"title":"A novel anticoagulant protein with high affinity to blood coagulation factor Va from Tegillarca granosa.","authors":"Won-Kyo Jung, Hee-Yeon Jo, Zhong-Ji Qian, Young-Ju Jeong, Sae-Gwang Park, Il-Whan Choi, Se-Kwon Kim","doi":"10.5483/bmbrep.2007.40.5.832","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.5.832","url":null,"abstract":"A novel inhibitory protein against blood coagulation factor Va (FVa) was purified from muscle protein of granulated ark (Tegillarca granosa, order Arcoida, marine bivalvia) by consecutive FPLC method using anion exchange and gel permeation chromatography. In the results of ESIQTOF tandem mass analysis and database research, it was revealed that the purified T. granosa anticoagulant protein (TGAP) has 7.7 kDa of molecular mass and its partial sequence, HTHLQRAPHPNALGYHGK, has a high identity (64%) with serine/threonine kinase derived from Rhodopirellula baltica (order Planctomycetales, marine bacteria). TGAP could potently prolong thrombin time (TT), corresponding to inhibition of thrombin (FIIa) formation. Specific factor inhibitory assay showed that TGAP inhibits FVa among the major components of prothrombinase complex. In vitro assay for direct-binding affinity using surface plasmon resonance (SPR) spectrometer indicated that TGAP could be directly bound with FVa. In addition, the binding affinity of FVa to FII was decreased by addition of TGAP in dose-dependant manner (IC50 value = 77.9 nM). These results illustrated that TGAP might interact with a heavy chain of FVa (FVa(H)) bound to FII in prothrombin complex. The present study elucidated that non-cytotoxic T. granosa anticoagulant protein (TGAP) bound to FVa can prolong blood coagulation time by inhibiting conversion of FII to FIIa in blood coagulation cascade. In addition, TGAP did not significantly (P < 0.05) show fibrinolytic activity and cytotoxicity on venous endothelial cell line (ECV 304).","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 5","pages":"832-8"},"PeriodicalIF":0.0,"publicationDate":"2007-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27038479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 41