Journal of biochemistry and molecular biology最新文献

The Role of Cellular Prion Protein in Immune System 细胞朊蛋白在免疫系统中的作用

Journal of biochemistry and molecular biology Pub Date : 2023-11-14 DOI: 10.5483/bmbrep.2023-0151

Seunghwa Cha, Mi-Yeon Kim

引用次数: 0

SARS-CoV-2 infection induces expression and secretion of lipocalin-2 and regulates iron in a human lung cancer xenograft model 在人肺癌异种移植模型中，SARS-CoV-2感染诱导脂钙素-2的表达和分泌并调节铁

Journal of biochemistry and molecular biology Pub Date : 2023-11-07 DOI: 10.5483/bmbrep.2023-0177

Sangkyu Park, Dongbum Kim, Jinsoo Kim, Hyung-Joo Kwon, Younghee Lee

{"title":"SARS-CoV-2 infection induces expression and secretion of lipocalin-2 and regulates iron in a human lung cancer xenograft model","authors":"Sangkyu Park, Dongbum Kim, Jinsoo Kim, Hyung-Joo Kwon, Younghee Lee","doi":"10.5483/bmbrep.2023-0177","DOIUrl":"https://doi.org/10.5483/bmbrep.2023-0177","url":null,"abstract":"Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection leads to various clinical symptoms including anemia. Lipocalin-2 has various biological functions, including defense against bacterial infections through iron sequestration, and it serves as a biomarker for kidney injury. In a human protein array, we observed increased lipocalin-2 expression due to parental SARS-CoV-2 infection in the Calu-3 human lung cancer cell line. The secretion of lipocalin-2 was also elevated in response to parental SARS-CoV-2 infection, and the SARS-CoV-2 Alpha, Beta, and Delta variants similarly induced this phenomenon. In a Calu-3 implanted mouse xenograft model, parental SARSCoV- 2 and Delta variant induced lipocalin-2 expression and secretion. Additionally, the iron concentration increased in the Calu-3 tumor tissues and decreased in the serum due to infection. In conclusion, SARS-CoV-2 infection induces the production and secretion of lipocalin-2, potentially resulting in a decrease in iron concentration in serum. Because the concentration of iron ions in the blood is associated with anemia, this phenomenon could contribute to developing anemia in COVID-19 patients.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"11 s1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135431347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MMPP is a novel VEGFR2 inhibitor that suppresses angiogenesis via VEGFR2/AKT/ERK/NF-κB pathway MMPP是一种新型的VEGFR2抑制剂，通过VEGFR2/AKT/ERK/NF-κB途径抑制血管生成

Journal of biochemistry and molecular biology Pub Date : 2023-11-03 DOI: 10.5483/bmbrep.2023-0150

Na-Yeon Kim, Hyo-Min Park, Jae-Young Park, Uijin Kim, Ha Youn Shin, Hee Pom Lee, Jin Tae Hong, Do-Young Yoon

{"title":"MMPP is a novel VEGFR2 inhibitor that suppresses angiogenesis via VEGFR2/AKT/ERK/NF-κB pathway","authors":"Na-Yeon Kim, Hyo-Min Park, Jae-Young Park, Uijin Kim, Ha Youn Shin, Hee Pom Lee, Jin Tae Hong, Do-Young Yoon","doi":"10.5483/bmbrep.2023-0150","DOIUrl":"https://doi.org/10.5483/bmbrep.2023-0150","url":null,"abstract":"Many types of cancer are associated with excessive angiogenesis. Anti-angiogenic treatment is an effective strategy for treating solid cancers. This study aimed to demonstrate the inhibitory effects of (E)-2-methoxy-4-(3-(4-methoxyphenyl) prop-1-en-1-yl) phenol (MMPP) in VEGFA-induced angiogenesis. The results indicated that MMPP effectively suppressed various angiogenic processes, such as cell migration, invasion, tube formation, and sprouting of new vessels in human umbilical vein endothelial cells (HUVECs) and mouse aortic ring. The inhibitory mechanism of MMPP on angiogenesis involves targeting VEGFR2. MMPP showed high binding affinity for the VEGFR2 ATP-binding domain. Additionally, MMPP improved VEGFR2 thermal stability and inhibited VEGFR2 kinase activity, suppressing the downstream VEGFR2/AKT/ERK pathway. MMPP attenuated the activation and nuclear translocation of NF-κB, and it downregulated NF-κB target genes such as VEGFA, VEGFR2, MMP2, and MMP9. Furthermore, conditioned medium from MMPP-treated breast cancer cells effectively inhibited angiogenesis in endothelial cells. These results suggested that MMPP had great promise as a novel VEGFR2 inhibitor with potent anti-angiogenic properties for cancer treatment via VEGFR2/AKT/ERK/NF-κB signaling pathway.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"15 9","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135874113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coordination chemistry of mitochondrial copper metalloenzymes: exploring implications for copper dyshomeostasis in cell death 线粒体铜金属酶的配位化学:探索细胞死亡中铜稳态失调的意义

Journal of biochemistry and molecular biology Pub Date : 2023-10-30 DOI: 10.5483/bmbrep.2023-0172

Daeun Shim, Jiyeon Han

{"title":"Coordination chemistry of mitochondrial copper metalloenzymes: exploring implications for copper dyshomeostasis in cell death","authors":"Daeun Shim, Jiyeon Han","doi":"10.5483/bmbrep.2023-0172","DOIUrl":"https://doi.org/10.5483/bmbrep.2023-0172","url":null,"abstract":"Mitochondria, fundamental cellular organelles that govern energy metabolism, hold a pivotal role in cellular vitality. While consuming dioxygen to produce adenosine triphosphate (ATP), the electron transfer process within mitochondria can engender the formation of reactive oxygen species that exert dual roles in endothelial homeostatic signaling and oxidative stress. In the context of the intricate electron transfer process, several metal ions that include copper, iron, zinc, and manganese serve as crucial cofactors in mitochondrial metalloenzymes to mediate the synthesis of ATP and antioxidant defense. In this mini review, we provide a comprehensive understanding of the coordination chemistry of mitochondrial cuproenzymes. In detail, superoxide dismutase 1 (SOD1) functions in detoxifying superoxide into hydrogen peroxide, while cytochrome c oxidase (CcO) reduces dioxygen to water coupled with proton pumping to generate an electrochemical gradient. With an emphasis on the catalytic reactions of the copper metalloenzymes and insights into their ligand environment, we also outline the metalation process of these enzymes throughout the copper trafficking system. The impairment of copper homeostasis can trigger mitochondrial dysfunction, and potentially lead to the development of copper-related disorders. We describe the current knowledge regarding copper-mediated toxicity mechanisms, thereby shedding light on prospective therapeutic strategies for pathologies intertwined with copper dyshomeostasis.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"34 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136105770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The N-terminal peptide of the main protease of SARS-CoV-2, targeting dimer interface, inhibits its proteolytic activity SARS-CoV-2主要蛋白酶的n端肽靶向二聚体界面，抑制其蛋白水解活性

Journal of biochemistry and molecular biology Pub Date : 2023-10-19 DOI: 10.5483/bmbrep.2023-0153

Sunyu song, Yeseul kim, Kiwoong kwak, Hyeonmin Lee, Hyunjae Park, Young Bong Kim, Hee Jung Lee, lin-woo kang

{"title":"The N-terminal peptide of the main protease of SARS-CoV-2, targeting dimer interface, inhibits its proteolytic activity","authors":"Sunyu song, Yeseul kim, Kiwoong kwak, Hyeonmin Lee, Hyunjae Park, Young Bong Kim, Hee Jung Lee, lin-woo kang","doi":"10.5483/bmbrep.2023-0153","DOIUrl":"https://doi.org/10.5483/bmbrep.2023-0153","url":null,"abstract":"The main protease (Mpro) of SARS-CoV-2 cleaves 11 sites of viral polypeptide chains and generates essential non-structural proteins for viral replication. Mpro is an important drug target against COVID-19. In this study, we developed a real-time fluorometric turn-on assay system to evaluate Mpro proteolytic activity for a substrate peptide between NSP4 and NSP5. It produced reproducible and reliable results suitable for HTS inhibitor assays. Thus far, most inhibitors against Mpro target the active site for substrate binding. Mpro exists as a dimer, which is essential for its activity. We investigated the potential of the Mpro dimer interface to act as a drug target. The dimer interface is formed of domain II and domain III of each protomer, in which N-terminal ten amino acids of the domain I are bound in the middle as a sandwich. The N-terminal part provides approximately 39% of the dimer interface between two protomers. In the real-time fluorometric turn-on assay system, peptides of the N-terminal ten amino acids, N10, can inhibit the Mpro activity. The dimer interface could be a prospective drug target against Mpro. The N-terminal sequence can help develop a potential inhibitor.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135667253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elevated level of PLRG1 is critical for the proliferation and maintenance of genome stability of tumor cells PLRG1水平的升高对肿瘤细胞的增殖和基因组稳定性的维持至关重要

Journal of biochemistry and molecular biology Pub Date : 2023-10-18 DOI: 10.5483/bmbrep.2023-0162

Hyunji Choi, Moonkyung Kang, Kee-Ho Lee, Yeon-Soo Kim

{"title":"Elevated level of PLRG1 is critical for the proliferation and maintenance of genome stability of tumor cells","authors":"Hyunji Choi, Moonkyung Kang, Kee-Ho Lee, Yeon-Soo Kim","doi":"10.5483/bmbrep.2023-0162","DOIUrl":"https://doi.org/10.5483/bmbrep.2023-0162","url":null,"abstract":"Pleiotropic Regulator 1 (PLRG1), a highly conserved element in the spliceosome, associates with Prp19, SPF27, and CDC5L to form a Ninety complex (NTC). This complex plays crucial roles in both pre-mRNA splicing and DNA repair processes. Here, we provide evidence that PLRG1 has a multifaceted impact on cancer cell proliferation. Comparing its expression in cancer and normal, we observed that PLRG1 is upregulated in various tumor tissues and cell lines. Knockdown of PLRG1 resulted in tumor-specific cell death. Depletion of PLRG1 leads to notable effects, including mitotic arrest, microtubule instability, endoplasmic reticulum (ER) stress, and accumulation of autophagy, ultimately culminating in apoptosis. Our findings also demonstrate that PLRG1 downregulation contributes to DNA damage in cancer cells, which we confirm through experimental validation as impairing DNA repair. Interestingly, when PLRG1 is decreased in normal cells, it induces G1 arrest as a self-protective mechanism, distinguishing it from the effects observed in cancer cells. These results highlight the multifaceted impacts of PLRG1 in cancer and underscore its potential as a novel anti-cancer strategy by selectively targeting cancer cells.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135824318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genistein alleviates pulmonary fibrosis by inactivating lung fibroblasts 染料木素通过灭活肺成纤维细胞减轻肺纤维化

Journal of biochemistry and molecular biology Pub Date : 2023-10-13 DOI: 10.5483/bmbrep.2023-0099

Kim Hak Su, Chung Hyunju, Seo Jung-Woo, Kwon Seung-hyun

{"title":"Genistein alleviates pulmonary fibrosis by inactivating lung fibroblasts","authors":"Kim Hak Su, Chung Hyunju, Seo Jung-Woo, Kwon Seung-hyun","doi":"10.5483/bmbrep.2023-0099","DOIUrl":"https://doi.org/10.5483/bmbrep.2023-0099","url":null,"abstract":"Pulmonary fibrosis is a serious lung disease that occurs predominantly in men. Genistein is an important natural soybeanderived phytoestrogen that affects various biological functions, such as cell migration and fibrosis. However, the antifibrotic effects of genistein on pulmonary fibrosis are largely unknown. The antifibrotic effects of genistein were evaluated using in vitro and in vivo models of lung fibrosis. Proteomic data were analyzed using nano-LC-ESI-MS/MS. Genistein significantly reduced transforming growth factor (TGF)-β1-induced expression of collagen type I and α-smooth muscle actin (SMA) in MRC-5 cells and primary fibroblasts from patients with idiopathic pulmonary fibrosis (IPF). Genistein also reduced TGF-β1-induced expression of p-Smad2/3 and p-p38 MAPK in fibroblast models. Comprehensive protein analysis confirmed that genistein exerted an anti-fibrotic effect by regulating various molecular mechanisms, such as unfolded protein response, epithelial mesenchymal transition (EMT), mTORC1 signaling, cell death, and several metabolic pathways. Genistein was also found to decrease hydroxyproline levels in the lungs of BLM-treated mice. Genistein exerted an anti-fibrotic effect by preventing fibroblast activation, suggesting that genistein could be developed as a pharmacological agent for the prevention and treatment of pulmonary fibrosis.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"118 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135804567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell RNA sequencing reveals heterogeneity of adipose tissue-derived mesenchymal stem cells under chondrogenic induction 单细胞RNA测序揭示了软骨诱导下脂肪组织来源的间充质干细胞的异质性

Journal of biochemistry and molecular biology Pub Date : 2023-10-12 DOI: 10.5483/bmbrep.2023-0161

Jeewan Chun, Ji-Hoi Moon, Kyu Hwan Kwack, Eun-Young Jang, Saebyeol Lee, Hak Kyun Kim, Jae-Hyung Lee

{"title":"Single-cell RNA sequencing reveals heterogeneity of adipose tissue-derived mesenchymal stem cells under chondrogenic induction","authors":"Jeewan Chun, Ji-Hoi Moon, Kyu Hwan Kwack, Eun-Young Jang, Saebyeol Lee, Hak Kyun Kim, Jae-Hyung Lee","doi":"10.5483/bmbrep.2023-0161","DOIUrl":"https://doi.org/10.5483/bmbrep.2023-0161","url":null,"abstract":"This study investigated how adipose tissue-derived mesenchymal stem cells (AT-MSCs) respond to chondrogenic induction using droplet-based single-cell RNA sequencing (scRNA-seq). We analyzed 37,219 high-quality transcripts from control cells and cells induced for 1 week (1W) and 2 weeks (2W). Four distinct cell clusters (0-3), undetectable by bulk analysis, exhibited varying proportions. Cluster 1 dominated in control and 1W cells, whereas cluster 3, 2, and 0 exclusively dominated in control, 1W, and 2W cells, respectively. Furthermore, heterogeneous chondrogenic markers expression within clusters emerged. Gene ontology (GO) enrichment analysis of differentially expressed genes unveiled cluster-specific variations in key biological processes (BP): (1) Cluster 1 exhibited upregulation of GO-BP terms related to ribosome biogenesis and translational control, crucial for maintaining stem cell properties and homeostasis; (2) Additionally, cluster 1 showed upregulation of GO-BP terms associated with mitochondrial oxidative metabolism; (3) Cluster 3 displayed upregulation of GO-BP terms related to cell proliferation; (4) Clusters 0 and 2 demonstrated similar upregulation of GO-BP terms linked to collagen fibril organization and supramolecular fiber organization. However, only cluster 0 showed a significant decrease in GO-BP terms related to ribosome production, implying a potential correlation between ribosome regulation and the differentiation stages of AT-MSCs. Overall, our findings highlight heterogeneous cell clusters with varying balances between proliferation and differentiation before and after chondrogenic stimulation. This provides enhanced insights into the single-cell dynamics of AT-MCSs during chondrogenic differentiation.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"162 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135968052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of CMGC kinases by hypoxia 缺氧对CMGC激酶的调节

Journal of biochemistry and molecular biology Pub Date : 2023-10-12 DOI: 10.5483/bmbrep.2023-0165

Sang Bae Lee, KyeongJin Kim

{"title":"Regulation of CMGC kinases by hypoxia","authors":"Sang Bae Lee, KyeongJin Kim","doi":"10.5483/bmbrep.2023-0165","DOIUrl":"https://doi.org/10.5483/bmbrep.2023-0165","url":null,"abstract":"Hypoxia, a widespread occurrence observed in various malignant tumors, results from rapid tumor growth that outpaces the oxygen supply. Tumor hypoxia precipitates several effects on tumor biology; these include activating angiogenesis, intensifying invasiveness, enhancing the survival of tumor cells, suppressing anti-tumor immunity, and fostering resistance to therapy. Aligned with the findings that correlate CMGC kinases with the regulation of Hypoxia-Inducible Factor (HIF), a pivotal modulator, reports also indicate that hypoxia governs the activity of CMGC kinases, including DYRK1 kinases. Prolyl hydroxylation of DYRK1 kinases by PHD1 constitutes a novel mechanism of kinase maturation and activation. This modification \"primes\" DYRK1 kinases for subsequent tyrosine autophosphorylation, a vital step in their activation cascade. This mechanism adds a layer of intricacy to comprehending the regulation of CMGC kinases, and underscores the complex interplay between distinct post-translational modifications in harmonizing precise kinase activity. Overall, hypoxia assumes a substantial role in cancer progression, influencing diverse aspects of tumor biology that include angiogenesis, invasiveness, cell survival, and resistance to treatment. CMGC kinases are deeply entwined in its regulation. To fathom the molecular mechanisms underpinning hypoxia's impact on cancer cells, comprehending how hypoxia and prolyl hydroxylation govern the activity of CMGC kinases, including DYRK1 kinases, becomes imperative. This insight may pave the way for pioneering therapeutic approaches that target the hypoxic tumor microenvironment and its associated challenges.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"136 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135963290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural studies of serotonin receptor family 血清素受体家族的结构研究

Journal of biochemistry and molecular biology Pub Date : 2023-10-11 DOI: 10.5483/bmbrep.2023-0147

Apeksha Parajulee, Kuglae Kim

{"title":"Structural studies of serotonin receptor family","authors":"Apeksha Parajulee, Kuglae Kim","doi":"10.5483/bmbrep.2023-0147","DOIUrl":"https://doi.org/10.5483/bmbrep.2023-0147","url":null,"abstract":"Serotonin receptors, also known as 5-HT receptors, belong to the G protein-coupled receptors (GPCRs) superfamily. They mediate the effects of serotonin, a neurotransmitter that plays a key role in a wide range of functions including mood regulation, cognition and appetite. The functions of serotonin are mediated by a family of 5-HT receptors including 12 GPCRs belonging to six major families: 5-HT1, 5-HT2, 5-HT4, 5-HT5, 5-HT6 and 5-HT7. Despite their distinct characteristics and functions, these receptors' subtypes share common structural features and signaling mechanisms. Understanding the structure, functions and pharmacology of the serotonin receptor family is essential for unraveling the complexities of serotonin signaling and developing targeted therapeutics for neuropsychiatric disorders. However, developing drugs that selectively target specific receptor subtypes is challenging due to the structural similarities in their orthosteric binding sites. This review focuses on the recent advancements in the structural studies of 5-HT receptors, highlighting the key structural features of each subtype and shedding light on their potential as targets for mental health and neurological disorders (such as depression, anxiety, schizophrenia, and migraine) drugs. [BMB Reports 2023; 56(10): 527-536].","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136059814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0