Journal of biochemistry and molecular biology最新文献_第2页

C-reactive protein accelerates DRP1-mediated mitochondrial fission by modulating ERK1/2-YAP signaling in cardiomyocytes. c反应蛋白通过调节心肌细胞ERK1/2-YAP信号加速drp1介导的线粒体分裂。

Journal of biochemistry and molecular biology Pub Date : 2023-10-05 DOI: 10.5483/bmbrep.2023-0127

Suyeon Jin, Chan Joo Lee, Gibbeum Lim, Sungha Park, Sang-Hak Lee, Ji Hyung Chung, Jaewon Oh, Seok-Min Kang

{"title":"C-reactive protein accelerates DRP1-mediated mitochondrial fission by modulating ERK1/2-YAP signaling in cardiomyocytes.","authors":"Suyeon Jin, Chan Joo Lee, Gibbeum Lim, Sungha Park, Sang-Hak Lee, Ji Hyung Chung, Jaewon Oh, Seok-Min Kang","doi":"10.5483/bmbrep.2023-0127","DOIUrl":"https://doi.org/10.5483/bmbrep.2023-0127","url":null,"abstract":"C-reactive protein (CRP) is an inflammatory marker and risk factor for atherosclerosis and cardiovascular diseases. However, the mechanism through which CRP induces myocardial damage remains unclear. This study aimed to determine how CRP damages cardiomyocytes via the change of mitochondrial dynamics and whether survivin, an anti-apoptotic protein, exerts a cardioprotective effect in this process. We treated H9c2 cardiomyocytes with CRP and found increased intracellular ROS production and shortened mitochondrial length. CRP treatment phosphorylated ERK1/2 and promoted increased expression, phosphorylation, and translocation of DRP1, a mitochondrial fission-related protein, from the cytoplasm to the mitochondria. The expression of mitophagy proteins PINK1 and PARK2 was also increased by CRP. YAP, a transcriptional regulator of PINK1 and PARK2, was also increased by CRP. Knockdown of YAP prevented CRP-induced increases in DRP1, PINK1, and PARK2. Furthermore, CRP-induced changes in the expression of DRP1 and increases in YAP, PINK1, and PARK2 were inhibited by ERK1/2 inhibition, suggesting that ERK1/2 signaling is involved in CRP-induced mitochondrial fission. We treated H9c2 cardiomyocytes with a recombinant TAT-survivin protein before CRP treatment, which reduced CRP-induced ROS accumulation and reduced mitochondrial fission. CRP-induced activation of ERK1/2 and increases in the expression and activity of YAP and its downstream mitochondrial proteins were inhibited by TAT-survivin. This study shows that mitochondrial fission occurs during CRPinduced cardiomyocyte damage and that the ERK1/2-YAP axis is involved in this process, and identifies that survivin alters these mechanisms to prevent CRP-induced mitochondrial damage.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134948219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct sets of lysosomal genes define synucleinopathy and tauopathy 不同的溶酶体基因决定了突触核蛋白病和tau病

Journal of biochemistry and molecular biology Pub Date : 2023-09-26 DOI: 10.5483/bmbrep.2023-0109

Kyu Won Oh, Dong-Kyu Kim, Allen Hsu, Seung-Jae Lee

{"title":"Distinct sets of lysosomal genes define synucleinopathy and tauopathy","authors":"Kyu Won Oh, Dong-Kyu Kim, Allen Hsu, Seung-Jae Lee","doi":"10.5483/bmbrep.2023-0109","DOIUrl":"https://doi.org/10.5483/bmbrep.2023-0109","url":null,"abstract":"Neurodegenerative diseases are characterized by distinct protein aggregates, such as those of α-synuclein and tau. Lysosomal defect is a key contributor to the accumulation and propagation of aberrant protein aggregates in these diseases. The discoveries of common proteinopathies in multiple forms of lysosomal storage diseases (LSDs) and the identification of some LSD genes as susceptible genes for those proteinopathies suggest causative links between LSDs and the proteinopathies. The present study hypothesized that defects in lysosomal genes will differentially affect the propagation of α-synuclein and tau proteins, thereby determining the progression of a specific proteinopathy. We established an imaging-based high-contents screening (HCS) system in Caenorhabditis elegans (C. elegans) model, by which the propagation of α-synuclein or tau is measured by fluorescence intensity. Using this system, we performed RNA interference (RNAi) screening to induce a wide range of lysosomal malfunction through knock down of 79 LSD genes, and to obtain the candidate genes with significant change in protein propagation. While some LSD genes commonly affected both α-synuclein and tau propagation, our study identified the distinct sets of LSD genes that differentially regulate the propagation of either α-synuclein or tau. The specificity and efficacy of these LSD genes were retained in the disease-related phenotypes, such as pharyngeal pumping behavior and life span. This study suggests that distinct lysosomal genes differentially regulate the propagation of α-synuclein and tau, and offer a steppingstone to understanding disease specificity.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134904252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effect of polysaccharide fractions from Radix A. sinensis against tert-butylhydroperoxide induced oxidative injury in murine peritoneal macrophages. 中药多糖对过氧化叔丁基诱导的小鼠腹腔巨噬细胞氧化损伤的保护作用。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.928

Xingbin Yang, Yan Zhao, You Lv, Ying Yang, Yun Ruan

引用次数: 19

Detection of antistaphylococcal and toxic compounds by biological assay systems developed with a reporter Staphylococcus aureus strain harboring a heat inducible promoter - lacZ transcriptional fusion. 利用含有热诱导启动子- lacZ转录融合的金黄色葡萄球菌报告菌株开发的生物检测系统检测抗葡萄球菌和有毒化合物。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.936

Palas Kumar Chanda, Tridib Ganguly, Malabika Das, Chia Yen Lee, Thanh T Luong, Subrata Sau

{"title":"Detection of antistaphylococcal and toxic compounds by biological assay systems developed with a reporter Staphylococcus aureus strain harboring a heat inducible promoter - lacZ transcriptional fusion.","authors":"Palas Kumar Chanda, Tridib Ganguly, Malabika Das, Chia Yen Lee, Thanh T Luong, Subrata Sau","doi":"10.5483/bmbrep.2007.40.6.936","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.6.936","url":null,"abstract":"Previously it was reported that promoter of groES-groEL operon of Staphylococcus aureus is induced by various cell-wall active antibiotics. In order to exploit the above promoter for identifying novel antistaphylococcal drugs, we have cloned the promoter containing region (P(g)) of groES-groEL operon of S. aureus Newman and found that the above promoter is induced by sublethal concentrations of many antibiotics including cell-wall active antibiotics. A reporter S. aureus RN4220 strain (designated SAU006) was constructed by inserting the P(g)-lacZ transcriptional fusion into its chromosome. Agarose-based assay developed with SAU006 shows that P(g) in single-copy is also induced distinctly by different classes of antibiotics. Data indicate that ciprofloxacin, rifampicin, ampicillin, and cephalothin are strong inducers, whereas, tetracycline, streptomycin and vancomycin induce the above promoter weakly. Sublethal concentrations of ciprofloxacin and ampicilin even have induced P(g) efficiently in microtiter plate grown SAU006. Additional studies show for the first time that above promoter is also induced weakly by arsenate salt and hydrogen peroxide. Taken together, we suggest that our simple and sensitive assay systems with SAU006 could be utilized for screening and detecting not only novel antistaphylococcal compounds but also different toxic chemicals.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 6","pages":"936-43"},"PeriodicalIF":0.0,"publicationDate":"2007-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41046529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Characterization of a stress-responsive ankyrin repeat-containing zinc finger protein of Capsicum annuum (CaKR1). 辣椒胁迫响应锚蛋白重复序列锌指蛋白(CaKR1)的研究。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.952

Eun Soo Seong, Doil Choi, Hye Sun Cho, Chun Keum Lim, Hye Jeong Cho, Myeong-Hyeon Wang

{"title":"Characterization of a stress-responsive ankyrin repeat-containing zinc finger protein of Capsicum annuum (CaKR1).","authors":"Eun Soo Seong, Doil Choi, Hye Sun Cho, Chun Keum Lim, Hye Jeong Cho, Myeong-Hyeon Wang","doi":"10.5483/bmbrep.2007.40.6.952","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.6.952","url":null,"abstract":"We isolated many genes induced from pepper cDNA microarray data following their infection with the soybean pustule pathogen Xanthomonas axonopodis pv. glycines 8ra. A full-length cDNA clone of the Capsicum annuum ankyrin-repeat domain C(3)H(1) zinc finger protein (CaKR1) was identified in a chili pepper using the expressed sequence tag (EST) database. The deduced amino acid sequence of CaKR1 showed a significant sequence similarity (46%) to the ankyrin-repeat protein in very diverse family of proteins of Arabidopsis. The gene was induced in response to various biotic and abiotic stresses in the pepper leaves, as well as by an incompatible pathogen, such as salicylic acid (SA) and ethephon. CaKR1 expression was highest in the root and flower, and its expression was induced by treatment with agents such as NaCl and methyl viologen, as well as by cold stresses. These results showed that CaKR1 fusion with soluble, modified green fluorescent protein (smGFP) was localized to the cytosol in Arabidopsis protoplasts, suggesting that CaKR1 might be involved in responses to both biotic and abiotic stresses in pepper plants.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 6","pages":"952-8"},"PeriodicalIF":0.0,"publicationDate":"2007-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41046531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

Inhibitory properties of nerve-specific human glutamate dehydrogenase isozyme by chloroquine. 氯喹对神经特异性谷氨酸脱氢酶同工酶的抑制作用。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.1077

Myung-Min Choi, Eun-A Kim, Soo Young Choi, Tae Ue Kim, Sung-Woo Cho, Seung-Ju Yang

{"title":"Inhibitory properties of nerve-specific human glutamate dehydrogenase isozyme by chloroquine.","authors":"Myung-Min Choi, Eun-A Kim, Soo Young Choi, Tae Ue Kim, Sung-Woo Cho, Seung-Ju Yang","doi":"10.5483/bmbrep.2007.40.6.1077","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.6.1077","url":null,"abstract":"Human glutamate dehydrogenase exists in hGDH1 (housekeeping isozyme) and in hGDH2 (nerve-specific isozyme), which differ markedly in their allosteric regulation. In the nervous system, GDH is enriched in astrocytes and is important for recycling glutamate, a major excitatory neurotransmitter during neurotransmission. Chloroquine has been known to be a potent inhibitor of house-keeping GDH1 in permeabilized liver and kidney-cortex of rabbit. However, the effects of chloroquine on nerve-specific GDH2 have not been reported yet. In the present study, we have investigated the effects of chloroquine on hGDH2 at various conditions and showed that chloroquine could inhibit the activity of hGDH2 at dose-dependent manner. Studies of the chloroquine inhibition on enzyme activity revealed that hGDH2 was relatively less sensitive to chloroquine inhibition than house-keeping hGDH1. Incubation of hGDH2 was uncompetitive with respect of NADH and non-competitive with respect of 2-oxoglutarate. The inhibitory effect of chloroquine on hGDH2 was abolished, although in part, by the presence of ADP and L-leucine, whereas GTP did not change the sensitivity to chloroquine inhibition. Our results show a possibility that chloroquine may be used in regulating GDH activity and subsequently glutamate concentration in the central nervous system.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 6","pages":"1077-82"},"PeriodicalIF":0.0,"publicationDate":"2007-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41044675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Antimicrobial activity of mupirocin, daptomycin, linezolid, quinupristin/dalfopristin and tigecycline against vancomycin-resistant enterococci (VRE) from clinical isolates in Korea (1998 and 2005). 1998年和2005年韩国临床分离的莫匹霉素、达托霉素、利奈唑胺、奎努普汀/达福普汀和替加环素对万古霉素耐药肠球菌(VRE)的抗菌活性

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.881

Do Kyung Lee, Yuna Kim, Kun Sup Park, Jae Wook Yang, Kyungjae Kim, Nam Joo Ha

{"title":"Antimicrobial activity of mupirocin, daptomycin, linezolid, quinupristin/dalfopristin and tigecycline against vancomycin-resistant enterococci (VRE) from clinical isolates in Korea (1998 and 2005).","authors":"Do Kyung Lee, Yuna Kim, Kun Sup Park, Jae Wook Yang, Kyungjae Kim, Nam Joo Ha","doi":"10.5483/bmbrep.2007.40.6.881","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.6.881","url":null,"abstract":"It is a hot clinical issue whether newly approved antimicrobial agents such as daptomycin, linezolid, quinupristin/dalfopristin (synercid) and tigecycline are active enough to be used for infections caused by vancomycin resistant bacteria. We performed susceptibility tests for mupirocin, which is in widespread clinical use in Korea, and four new antimicrobials, daptomycin, linezolid, quinupristin/dalfopristin and tigecycline, against vancomycin-resistant Enterococcus faecalis and Enterococcus faecium isolated from Korean patients in 1998 and 2005 to evaluate and compare the in vitro activity of these antimicrobials. Among these agents, quinupristin/dalfopristin, which is rarely used in hospitals in Korea, showed relatively high resistance to several vancomycin-resistant enterococci (VRE) isolated in 2005. Likewise, daptomycin, linezolid and tigecycline have not yet been in clinical use in Korea. However, our results showed that most of the 2005 VRE isolates were already resistant to linezolid and daptomycin (highest minimum inhibitory concentration (MIC) value >100 microg/ml). Compared with the other four antimicrobial agents tested in this study, tigecycline generally showed the greatest activity against VRE. However, four strains of 2005 isolates exhibited resistance against tigecycline (MIC >12.5 microg/ml). Almost all VRE were resistant to mupirocin, whereas all E. faecium isolated in 1998 were inhibited at concentrations between 0.8 to approximately 1.6 microg/ml. In conclusion, resistances to these new antimicrobial agents were exhibited in most of VRE strains even though these new antibiotics have been rarely used in Korean hospitals.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 6","pages":"881-7"},"PeriodicalIF":0.0,"publicationDate":"2007-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41044904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Differential regulation of the promoter activity of the mouse UCP2 and UCP3 genes by MyoD and myogenin. MyoD和myogenin对小鼠UCP2和UCP3基因启动子活性的差异调控。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.921

Dongho Kim, Sarawut Jitrapakdee, Mary Thompson

{"title":"Differential regulation of the promoter activity of the mouse UCP2 and UCP3 genes by MyoD and myogenin.","authors":"Dongho Kim, Sarawut Jitrapakdee, Mary Thompson","doi":"10.5483/bmbrep.2007.40.6.921","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.6.921","url":null,"abstract":"UCP2 and UCP3 are members of the uncoupling protein family, which may play roles in energy homeostasis. In order to determine the regulation of the predominant expression of UCP3 in skeletal muscle, the effects of differentiation and myogenic regulatory factors on the promoter activities of the mouse UCP2 and UCP3 genes were studied. Reporter plasmids, containing approximately 3 kb of the 5'-upstream region of the mouse UCP2 and UCP3 genes, were transfected into C2C12 myoblasts, which were then induced to differentiate. Differentiation positively induced the reporter expression about 20-fold via the UCP3 promoter, but by only 2-fold via the UCP2 promoter. C2C12 myoblasts were cotransfected with expression vectors for myogenin and/or MyoD as well as reporter constructs. The simultaneous expression of myogenin and MyoD caused an additional 20-fold increase in the reporter expression via the UCP3 promoter, but only a weak effect via the UCP2 promoter. In L6 myoblasts, only MyoD activated the UCP3 promoter, but in 3T3-L1 cells neither factor activated the UCP3 promoter, indicating that additional cofactors are required, which are present only in C2C12 myoblasts. The expression of UCP2 and UCP3 is differentially regulated during muscle differentiation due to the different responsiveness of their promoter regions to myogenin and MyoD.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 6","pages":"921-7"},"PeriodicalIF":0.0,"publicationDate":"2007-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41044909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Molecular cloning and functional analysis of the gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase from hazel (Corylus avellana L. Gasaway). 榛子3-羟基-3-甲基戊二酰辅酶A还原酶基因的克隆及功能分析。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.861

Yechun Wang, Binhui Guo, Fei Zhang, Hongyan Yao, Zhiqi Miao, Kexuan Tang

{"title":"Molecular cloning and functional analysis of the gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase from hazel (Corylus avellana L. Gasaway).","authors":"Yechun Wang, Binhui Guo, Fei Zhang, Hongyan Yao, Zhiqi Miao, Kexuan Tang","doi":"10.5483/bmbrep.2007.40.6.861","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.6.861","url":null,"abstract":"The enzyme 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR; EC1.1.1.34) catalyzes the first committed step of isoprenoids biosynthesis in MVA pathway. Here we report for the first time the cloning and characterization of a full-length cDNA encoding HMGR (designated as CgHMGR, GenBank accession number EF206343) from hazel (Corylus avellana L. Gasaway), a taxol-producing plant species. The full-length cDNA of CgHMGR was 2064 bp containing a 1704-bp ORF encoding 567 amino acids. Bioinformatic analyses revealed that the deduced CgHMGR had extensive homology with other plant HMGRs and contained two transmembrane domains and a catalytic domain. The predicted 3-D model of CgHMGR had a typical spatial structure of HMGRs. Southern blot analysis indicated that CgHMGR belonged to a small gene family. Expression analysis revealed that CgHMGR expressed high in roots, and low in leaves and stems, and the expression of CgHMGR could be up-regulated by methyl jasmonate (MeJA). The functional color assay in Escherichia coli showed that CgHMGR could accelerate the biosynthesis of beta-carotene, indicating that CgHMGR encoded a functional protein. The cloning, characterization and functional analysis of CgHMGR gene will enable us to further understand the role of CgHMGR involved in taxol biosynthetic pathway in C. avellana at molecular level.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 6","pages":"861-9"},"PeriodicalIF":0.0,"publicationDate":"2007-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41045551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 41

Cell cycle regulation and induction of apoptosis by beta-carotene in U937 and HL-60 leukemia cells. β -胡萝卜素对U937和HL-60白血病细胞周期调控及诱导凋亡的作用。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.1009

K R Upadhyaya, K S Radha, H K Madhyastha

{"title":"Cell cycle regulation and induction of apoptosis by beta-carotene in U937 and HL-60 leukemia cells.","authors":"K R Upadhyaya, K S Radha, H K Madhyastha","doi":"10.5483/bmbrep.2007.40.6.1009","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.6.1009","url":null,"abstract":"In this communication, we report the efficacy of beta-carotene towards differentiation and apoptosis of leukemia cells. Dose (20 microM) and time dependence (12 h) tests of beta- carotene showed a higher magnitude of decrease (significance p < 0.05) in cell numbers and cell viability in HL-60 cells than U937 cells but not normal cell like Peripheral blood mononuclear cell (PBMC). Microscopical observation of beta-carotene treated cells showed a distinct pattern of morphological abnormalities with inclusion of apoptotic bodies in both leukemia cell lines. When cells were treated with 20 microM of beta-carotene, total genomic DNA showed a fragmentation pattern and this pattern was clear in HL-60 than U937 cells. Both the cell lines, on treatment with beta- carotene, showed a clear shift in G(1) phase of the cell cycle. In addition the study also revealed anti-oxidant properties of beta-carotene since there was reduction in relative fluorescent when treated than the control at lower concentration. Collectively this study shows the dual phenomenon of apoptosis and differentiation of leukemia cells on treatment with beta-carotene.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 6","pages":"1009-15"},"PeriodicalIF":0.0,"publicationDate":"2007-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41045907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36