Journal of biochemistry and molecular biology最新文献_第3页

Construction and expression of mutant cDNAs responsible for genetic polymorphism in aldehyde oxidase in Donryu strain rats. 东流品系大鼠醛氧化酶基因多态性突变cdna的构建与表达。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.1021

Mayuko Adachi, Kunio Itoh, Akiko Masubuchi, Nobuaki Watanabe, Yorihisa Tanaka

{"title":"Construction and expression of mutant cDNAs responsible for genetic polymorphism in aldehyde oxidase in Donryu strain rats.","authors":"Mayuko Adachi, Kunio Itoh, Akiko Masubuchi, Nobuaki Watanabe, Yorihisa Tanaka","doi":"10.5483/bmbrep.2007.40.6.1021","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.6.1021","url":null,"abstract":"We demonstrated the genetic polymorphism of aldehyde oxidase (AO) in Donryu strain rats: the ultrarapid metabolizer (UM) with nucleotide mutation of (377G, 2604C) coding for amino acid substitution of (110Gly, 852Val), extensive metabolizer (EM) with (377G/A, 2604C/T) coding for (110Gly/Ser, 852Val/Ala), and poor metabolizer (PM) with (377A, 2604T) coding for (110Ser, 852Ala), respectively. The results suggested that 377G > A and/or 2604C > T should be responsible for the genetic polymorphism. In this study, we constructed an E. coli expression system of four types of AO cDNA including Mut-1 with (377G, 2604T) and Mut-2 with (377A, 2604C) as well as naturally existing nucleotide sequences of UM and PM in order to clarify which one is responsible for the polymorphism. Mut-1 and Mut-2 showed almost the same high and low activity as that of the UM and PM groups, respectively. Thus, the expression study of mutant AO cDNA directly revealed that the nucleotide substitution of 377G > A, but not that of 2604C > T, will play a critical role in the genetic polymorphism of AO in Donryu strain rats. The reason amino acid substitution will cause genetic polymorphism in AO activity was discussed.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 6","pages":"1021-7"},"PeriodicalIF":0.0,"publicationDate":"2007-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41045909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Evidence of tandem repeat and extra thiol-groups resulted in the polymeric formation of bovine haptoglobin: a unique structure of Hp 2-2 phenotype. 串联重复和额外的巯基导致牛接触珠蛋白聚合形成的证据:Hp 2-2表型的独特结构。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.1028

Yi An Lai, I Hsiang Lai, Chi Feng Tseng, James Lee, Simon J T Mao

{"title":"Evidence of tandem repeat and extra thiol-groups resulted in the polymeric formation of bovine haptoglobin: a unique structure of Hp 2-2 phenotype.","authors":"Yi An Lai, I Hsiang Lai, Chi Feng Tseng, James Lee, Simon J T Mao","doi":"10.5483/bmbrep.2007.40.6.1028","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.6.1028","url":null,"abstract":"Human plasma Hp is classified as 1-1, 2-1, and 2-2. They are inherited from two alleles Hp 1 and Hp 2, but there is only Hp 1 in almost all the animal species. Hp 2-2 molecule is extremely large and heterogeneous associated with the development of inflammatory-related diseases. In this study, we expressed entire bovine Hp in E. coli as a alphabeta linear form. Interestingly, the antibodies prepared against this form could recognize the subunit of native Hp. In stead of a complicated column method, the antibody was able to isolate bovine Hp via immunoaffinity and gel-filtration columns. The isolated Hp is polymeric containing two major molecular forms (660 and 730 kDa). Their size and hemoglobin binding complex are significantly larger than that of human Hp 2-2. The amino-acid sequence deducted from the nucleotide sequence is similar to human Hp 2 containing a tandem repeat over the alpha chain. Thus, the Hp 2 allele is not unique in human. We also found that there is one additional -SH group (Cys-97) in bovine alpha chain with a total of 8 -SH groups, which may be responsible for the overall polymeric structure that is markedly different from human Hp 2-2. The significance of the finding and its relationship to structural evolution are also discussed.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 6","pages":"1028-38"},"PeriodicalIF":0.0,"publicationDate":"2007-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41045910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Lipase inactive mutant of PLC-gamma1 regulates NGF-induced neurite outgrowth via enzymatic activity and regulation of cell cycle regulatory proteins. 脂肪酶失活突变体PLC-gamma1通过酶活性和细胞周期调节蛋白调控ngf诱导的神经突生长。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.888

Truong Le Xuan Nguyen, Jee-Yin Ahn

{"title":"Lipase inactive mutant of PLC-gamma1 regulates NGF-induced neurite outgrowth via enzymatic activity and regulation of cell cycle regulatory proteins.","authors":"Truong Le Xuan Nguyen, Jee-Yin Ahn","doi":"10.5483/bmbrep.2007.40.6.888","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.6.888","url":null,"abstract":"Src homology (SH) domains of phospholipase C-gamma1 (PLC-gamma1) impair NGF-mediated PC12 cells differentiation. However, whether the enzymatic activity is also implicated in this process remains elusive. Here, we report that the enzymatic activity of phospholipase C-gamma1 (PLC-gamma1) is at least partially involved to the blockage of neuronal differentiation via an abrogation of MAPK activation, as well as sustained Akt activation. By contrast, Overexpression of WT-PLC-gamma1 exhibited sustained NGF-induced MAPK activation, and triggered transient Akt activation resulting in profound inhibition of neurite outgrowth. However, lipase-inactive mutant (LIM) PLC-gamma1 cells fail to suppress neurite outgrowth, although it contains intact SH domains, specifically enhancing the expression of cyclin D1 and p21 proteins, which regulate the function of retinoblastoma Rb protein. These observations show that the lipase inactive mutant of PLC-gamma1 does not alter NGF-induced neuronal differentiation via enzymatic inability and the odulation of cell cycle regulatory proteins independent on SH3 domain.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 6","pages":"888-94"},"PeriodicalIF":0.0,"publicationDate":"2007-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41044905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Isolation and characterization of two Korean mistletoe lectins. 两种朝鲜槲寄生凝集素的分离与鉴定。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.959

Tae Bong Kang, Seong Kyu Song, Taek Joon Yoon, Yung Choon Yoo, Kwan Hee Lee, Erk Her, Jong Bae Kim

引用次数: 16

Cell selectivity of an antimicrobial peptide melittin diastereomer with D-amino acid in the leucine zipper sequence. 抗菌肽蜂毒素与亮氨酸拉链序列中d -氨基酸非对映体的细胞选择性。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.1090

Wan Long Zhu, Yong Hai Nan, Kyung-Soo Hahm, Song Yub Shin

引用次数: 44

Cloning and characterization of a PI-like MADS-box gene in Phalaenopsis orchid. 蝴蝶兰中类似 PI 的 MADS-box 基因的克隆和特征描述。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.845

Bin Guo, Saiyin Hexige, Tian Zhang, Jon K Pittman, Donghong Chen, Feng Ming

引用次数: 15

Proteomic analysis of O-GlcNAc modifications derived from streptozotocin and glucosamine induced beta-cell apoptosis. 链脲佐菌素和葡萄糖胺诱导β细胞凋亡的O-GlcNAc修饰的蛋白质组学分析。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.1058

Jungeun Park, Hyejin Kwon, Yup Kang, Youngsoo Kim

{"title":"Proteomic analysis of O-GlcNAc modifications derived from streptozotocin and glucosamine induced beta-cell apoptosis.","authors":"Jungeun Park, Hyejin Kwon, Yup Kang, Youngsoo Kim","doi":"10.5483/bmbrep.2007.40.6.1058","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.6.1058","url":null,"abstract":"The post-translational modifications of Ser and Thr residues by O-linked beta-N-acetylglucosamine (O-GlcNAc), i.e., O-GlcNAcylation, is considered a key means of regulating signaling, in a manner analogous to protein phosphorylation. Furthermore, it has been suggested that the increased flux of glucose through the hexosamine biosynthetic pathway (HBP) stimulates O-GlcNAcylation, and that this may be responsible for many of the manifestations of type 2 diabetes mellitus. To determine whether excessive O-GlcNAcylation of target proteins results in pancreatic beta cell dysfunction, we increased nucleocytoplasmic protein O-GlcNAcylation levels in beta cells by exposing them to streptozotocin and/or glucosamine. Streptozotocin and glucosamine co-treatment increased OGlcNAcylated proteomic patterns as assessed by immunoblotting, and these increases in nuclear and cytoplasmic protein O-GlcNAcylations were accompanied by impaired insulin secretion and enhanced apoptosis in pancreatic beta cells. This observed beta cell dysfunction prompted us to examine Akt and Bcl-2 family member proteins to determine which proteins are O-GlcNAcylated under conditions of high HBP throughput, and how these proteins are associated with beta cell apoptosis. Eventually, we identified ten new O-GlcNAcylated proteins that were expressed during beta cell apoptosis, and analyzed the functional implications of these proteins in relation to pancreatic beta cell dysfunction.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 6","pages":"1058-68"},"PeriodicalIF":0.0,"publicationDate":"2007-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41045913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Induction of growth hormone release by glycyrrhizae radix on rat. 甘草对大鼠生长激素释放的诱导作用。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.979

Ho Young Lee, Dae Young Jung, Hyekyung Ha, Sam Sik Kang, Ju Sun Kim, Chungsook Kim

{"title":"Induction of growth hormone release by glycyrrhizae radix on rat.","authors":"Ho Young Lee, Dae Young Jung, Hyekyung Ha, Sam Sik Kang, Ju Sun Kim, Chungsook Kim","doi":"10.5483/bmbrep.2007.40.6.979","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.6.979","url":null,"abstract":"Induction of growth hormone (GH) by Glycyrrhizae Radix (GR), one of the most popular herbal medicine, and its major ingredients were studied in rat pituitary cells in vitro and in vivo assay. The MeOH extract and the n-hexane (HX) fraction of GR induced rat GH (rGH) release up to 1.89 times (0.34 +/- 0.04 nM) and 4.59 times (0.83 +/- 0.03 nM), compared to the basal level (p < 0.05). Among many ingredients isolated and purified from GR both glycyrrhetinic acid and glycyrrhizin induced significantly rGH release compared to the control (p < 0.05). After an intravenous injection of rat growth hormone releasing hormone (rGHRH) (10 microg/kg) as positive control, in SD rats, Tmax of plasma rGH level was 10 min, C(max) was 3.84 +/- 0.01 nM (n = 3), and enhanced plasma rGH level returned to the baseline in 90 min. Both AUC(0-90) (area under the curve) of plasma rGH level after HX fraction and that after rGHRH administration were increased significantly from the basal level, respectively (p < 0.01). In conclusions, HX fraction is the most active fraction of MeOH extract of GR in rGH induction.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 6","pages":"979-85"},"PeriodicalIF":0.0,"publicationDate":"2007-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41046535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Overexpression and purification of PreS region of hepatitis B virus antigenic surface protein adr subtype in Escherichia coli. 乙型肝炎病毒抗原表面蛋白adr亚型PreS区在大肠杆菌中的过表达和纯化。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.1002

Naaz Abbas, Aftab Ahmad, Abdul Rauf Shakoori

引用次数: 7

PD184352 releases the regular hypoxic reversible DNA replication arrest in T24 cells. PD184352在T24细胞中释放常规的缺氧可逆DNA复制阻滞。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.895

Leenus Martin

引用次数: 1