Journal of biochemistry and molecular biology最新文献_第4页

RGS protein specificity towards Gq- and Gi/o-mediated ERK 1/2 and Akt activation, in vitro. RGS蛋白对Gq-和Gi/o介导的ERK 1/2和Akt激活的特异性。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.899

Thomas Anger, Nils Klintworth, Christian Stumpf, Werner G Daniel, Ulrike Mende, Christoph D Garlichs

{"title":"RGS protein specificity towards Gq- and Gi/o-mediated ERK 1/2 and Akt activation, in vitro.","authors":"Thomas Anger, Nils Klintworth, Christian Stumpf, Werner G Daniel, Ulrike Mende, Christoph D Garlichs","doi":"10.5483/bmbrep.2007.40.6.899","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.6.899","url":null,"abstract":"Extracellular Regulated Kinases (ERK) and Protein Kinase B (Akt) are intermediaries in relaying extracellular growth signals to intracellular targets. Each pathway can become activated upon stimulation of G protein-coupled receptors mediated by G(q) and G(i/o) proteins subjected to regulation by RGS proteins. The goal of the study was to delineate the specificity in which cardiac RGS proteins modulate G(q)and G(i/o)-induced ERK and Akt phosphorylation. To isolate G(q)- and G(i/o)-mediated effects, we exclusively expressed muscarinic M(2) or M(3) receptors in COS-7 cells. Western blot analyses demonstrated increase of phosphorylation of ERK 1.7-/3.3-fold and Akt 2.4-/6-fold in M(2)-/M(3)- expressing cells through carbachol stimulation. In co-expressions, M(3)/G(q)-induced activation of Akt was exclusively blunted through RGS3s/RGS3, whereas activation of ERK was inhibited additionally through RGS2/RGS5. M(2)/G(i/o) induced Akt activation was inhibited by all RGS proteins tested. RGS2 had no effect on M(2)/G(i/o)-induced ERK activation. The high degree of specificity in RGS proteins-depending modulation of G(q)- and G(i/o)-mediated ERK and Akt activation in the muscarinic network cannot merely be attributed exclusively to RGS protein selectivity towards G(q) or G(i/o) proteins. Counter-regulatory mechanisms and inter-signaling cross-talk may alter the sensitivity of GPCR-induced ERK and Akt activation to RGS protein regulation.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 6","pages":"899-910"},"PeriodicalIF":0.0,"publicationDate":"2007-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41044907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 49

Differentially expressed genes under cold acclimation in Physcomitrella patens. 小壶菌冷驯化下的差异表达基因。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.986

Ming-Ming Sun, Lin-Hui Li, Hua Xie, Rong-Cai Ma, Yi-Kun He

引用次数: 23

The important anti-apoptotic role and its regulation mechanism of PTTG1 in UV-induced apoptosis. PTTG1在紫外线诱导的细胞凋亡中的重要抗凋亡作用及其调控机制。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.966

Yongqing Lai, Dianqi Xin, Junhai Bai, Zebin Mao, Yanqun Na

引用次数: 17

AtHAP3b plays a crucial role in the regulation of flowering time in Arabidopsis during osmotic stress. AtHAP3b在渗透胁迫下拟南芥开花时间的调控中起关键作用。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.1083

Nai-Zhi Chen, Xiu-Qing Zhang, Peng-Cheng Wei, Qi-Jun Chen, Fei Ren, Jia Chen, Xue-Chen Wang

{"title":"AtHAP3b plays a crucial role in the regulation of flowering time in Arabidopsis during osmotic stress.","authors":"Nai-Zhi Chen, Xiu-Qing Zhang, Peng-Cheng Wei, Qi-Jun Chen, Fei Ren, Jia Chen, Xue-Chen Wang","doi":"10.5483/bmbrep.2007.40.6.1083","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.6.1083","url":null,"abstract":"The HAP complex has been found in many eukaryotic organisms. HAP recognizes the CCAAT box present in the promoters of 30% of all eukaryotic genes. The HAP complex consists of three subunits--HAP2, HAP3 and HAP5. In this paper, we report the biological function of the AtHAP3b gene that encodes one of the HAP3 subunits in Arabidopsis. Compared with wild-type plants, hap3b-1 and hap3b-2 mutants exhibited a delayed flowering time under long-day photoperiod conditions. Moreover, the transcription levels of FT were substantially lower in the mutants than in the wild-type plants. These results imply that AtHAP3b may function in the control of flowering time by regulating the expression of FT in Arabidopsis. In a subsequent study, AtHAP3b was found to be induced by osmotic stress. Under osmotic stress conditions, the hap3b-1 and hap3b-2 mutants flowered considerably later than the wild-type plants. These results suggest that the AtHAP3b gene plays more important roles in the control of lowering under osmotic stress in Arabidopsis.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 6","pages":"1083-9"},"PeriodicalIF":0.0,"publicationDate":"2007-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41044676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 79

Induction of megakaryocytic differentiation in chronic myelogenous leukemia cell K562 by 3-hydrogenkwadaphnin. 3-氢芥黄素诱导慢性髓性白血病细胞K562的巨核细胞分化。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.944

Azadeh Meshkini, Razieh Yazdanparast

{"title":"Induction of megakaryocytic differentiation in chronic myelogenous leukemia cell K562 by 3-hydrogenkwadaphnin.","authors":"Azadeh Meshkini, Razieh Yazdanparast","doi":"10.5483/bmbrep.2007.40.6.944","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.6.944","url":null,"abstract":"3-Hydrogenkwadaphnin (3-HK) is a daphnane-type diterpene ester isolated from Dendrostellera lessertii (Thymelaeaceae) with high differentiation and apoptotic potency in leukemic cells without any measurable adverse effects on normal cells (Moosavi et al., 2005b). In this study, we report that 3-HK (12 nM) has the ability to cease proliferation, induce differentiation and apoptosis in chronic myelogenous leukemia (CML) K562 cell line. The treated cells lost erythroid properties and differentiated along the megakaryocytic lineage based on the morphological features apparent after Wright-Giemsa staining, DNA content analysis and the expression of cell surface marker glycoprotein IIb as analyzed by flow cytometry. Moreover, using Hoechst 33258 and Annexin V double staining indicated the occurrence of apoptosis among the treated cells. On the other hand, restoration of the depleted GTP pool size by exogenous addition of guanosine (50 microM) reduced the effect of the drug regarding the extent of differentiation while no further enhancement of 3-HK effect was obtained by addition of exogenous hypoxanthine (100 microM). These interesting results necessitate further investigation regarding the mechanism of action of this unique anti-leukemic agent.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 6","pages":"944-51"},"PeriodicalIF":0.0,"publicationDate":"2007-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41046530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Disulfiram suppresses invasive ability of osteosarcoma cells via the inhibition of MMP-2 and MMP-9 expression. 双硫仑通过抑制MMP-2和MMP-9的表达来抑制骨肉瘤细胞的侵袭能力。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.1069

Hyun-Ji Cho, Tae-Sung Lee, Jae-Bok Park, Kwan-Kyu Park, Jung-Yoon Choe, Doo-Il Sin, Yoon-Yub Park, Yong-Suk Moon, Kwang-Gill Lee, Joo-Hong Yeo, Sang-Mi Han, Young-Su Cho, Myeong-Rak Choi, Nam-Gyu Park, Yun-Sik Lee, Young-Chae Chang

{"title":"Disulfiram suppresses invasive ability of osteosarcoma cells via the inhibition of MMP-2 and MMP-9 expression.","authors":"Hyun-Ji Cho, Tae-Sung Lee, Jae-Bok Park, Kwan-Kyu Park, Jung-Yoon Choe, Doo-Il Sin, Yoon-Yub Park, Yong-Suk Moon, Kwang-Gill Lee, Joo-Hong Yeo, Sang-Mi Han, Young-Su Cho, Myeong-Rak Choi, Nam-Gyu Park, Yun-Sik Lee, Young-Chae Chang","doi":"10.5483/bmbrep.2007.40.6.1069","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.6.1069","url":null,"abstract":"Cancer cells, characterized by local invasion and distant metastasis, are very much dependent on the extracellular matrix. The expression of matrix metalloproteinases (MMPs) has been implicated in the invasion and metastasis of cancer cells. In this study, we reported the effects of disulfiram, a clinically used anti-alcoholism drug, on tumor invasion suppression, as well as its effects on the activity of MMP-2 and MMP-9 in human osteosarcoma cells (U2OS). Disulfiram has been used for alcohol aversion therapy. However, recent reports have shown that disulfiram may have potential in the treatment of human cancers. Herewith, we showed that the anti-tumor effects of disulfiram, in an invasion assay using U2OS cells and that disulfiram has a type IV collagenase inhibitory activity that inhibits expression of genes and proteins responsible for both cell and non-cell mediated invasion on pathways. In conclusion, disulfiram inhibited expression of MMP-2 and MMP-9 and it regulated the invasion of human osteosarcoma cells. These observations raise the possibility of disulfiram being used clinical for the inhibition of cancer invasion.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 6","pages":"1069-76"},"PeriodicalIF":0.0,"publicationDate":"2007-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41044248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 95

Induction of growth hormone release by dioscin from Dioscorea batatas DECNE. 薯蓣皂苷对生长激素释放的诱导作用。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.1016

Ho Young Lee, Dae Young Jung, Hyekyung Ha, Kun-Ho Son, Su-Jin Jeon, Chungsook Kim

引用次数: 18

Ectopic expression of mitochondria endonuclease Pnu1p from Schizosaccharomyces pombe induces cell death of the yeast. 裂糖酵母线粒体内切酶Pnu1p的异位表达可诱导酵母细胞死亡。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.1095

Kaoru Oda, Nami Kawasaki, Masashi Fukuyama, Shogo Ikeda

{"title":"Ectopic expression of mitochondria endonuclease Pnu1p from Schizosaccharomyces pombe induces cell death of the yeast.","authors":"Kaoru Oda, Nami Kawasaki, Masashi Fukuyama, Shogo Ikeda","doi":"10.5483/bmbrep.2007.40.6.1095","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.6.1095","url":null,"abstract":"Endonuclease G (EndoG) is a mitochondrial non-specific nuclease that is highly conserved among the eukaryotes. Although the precise role of EndoG in mitochondria is not yet known, the enzyme is released from the mitochondria and digests nuclear DNA during apoptosis in mammalian cells. Schizosaccharomyces pombe has an EndoG homolog Pnu1p (previously named SpNuc1) that is produced as a precursor protein with a mitochondrial targeting sequence. During the sorting into mitochondria the signal sequence is cleaved to yield the functionally active endonuclease. From the analogy to EndoG, active extramitochondrial Pnu1p may trigger cell killing by degrading nuclear DNA. Here, we tested this possibility by expressing a truncated Pnu1p lacking the signal sequence in the extramitochondrial region of pnu1-deleted cells. The truncated Pnu1p was localized in the cytosol and nuclei of yeast cells. And ectopic expression of active Pnu1p led to cell death with fragmentation of nuclear DNA. This suggests that the Pnu1p is possibly involved in a certain type of yeast cell death via DNA fragmentation. Although expression of human Bak in S. pombe was lethal, Pnu1p nuclease is not necessary for hBak-induced cell death.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 6","pages":"1095-9"},"PeriodicalIF":0.0,"publicationDate":"2007-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41044678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Biosynthesis of isoprenoids: characterization of a functionally active recombinant 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (IspD) from Mycobacterium tuberculosis H37Rv. 类异戊二烯的生物合成:结核分枝杆菌H37Rv中功能活性重组2- c -甲基- d -赤藓糖醇4-磷酸胞基转移酶(IspD)的表征

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.911

Wenjun Shi, Jianfang Feng, Min Zhang, Xuhui Lai, Shengfeng Xu, Xuelian Zhang, Honghai Wang

{"title":"Biosynthesis of isoprenoids: characterization of a functionally active recombinant 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (IspD) from Mycobacterium tuberculosis H37Rv.","authors":"Wenjun Shi, Jianfang Feng, Min Zhang, Xuhui Lai, Shengfeng Xu, Xuelian Zhang, Honghai Wang","doi":"10.5483/bmbrep.2007.40.6.911","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.6.911","url":null,"abstract":"Tuberculosis, caused by Mycobacterium tuberculosis, continues to be one of the leading infectious diseases to humans. It is urgent to discover novel drug targets for the development of antitubercular agents. The 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway for isoprenoid biosynthesis has been considered as an attractive target for the discovery of novel antibiotics for its essentiality in bacteria and absence in mammals. MEP cytidyltransferase (IspD), the third-step enzyme of the pathway, catalyzes MEP and CTP to form 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME) and PPi. In the work, ispD gene from M. tuberculosis H37Rv (MtIspD) was cloned and expressed. With N-terminal fusion of a histidine-tagged sequence, MtIspD could be purified to homogeneity by one-step nickel affinity chromatography. MtIspD exists as a homodimer with an apparent molecular mass of 52 kDa. Enzyme property analysis revealed that MtIspD has high specificity for pyrimidine bases and narrow divalent cation requirements, with maximal activity found in the presence of CTP and Mg(2+). The turnover number of MtIspD is 3.4 s(-1). The Km for MEP and CTP are 43 and 92 muM, respectively. Furthermore, MtIspD shows thermal instable above 50 degrees C. Circular dichroism spectra revealed that the alteration of tertiary conformation is closely related with sharp loss of enzyme activity at higher temperature. This study is expected to help better understand the features of IspD and provide useful information for the development of novel antibiotics to treat M. tuberculosis.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 6","pages":"911-20"},"PeriodicalIF":0.0,"publicationDate":"2007-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41044908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Characterization of uridine-diphosphate dependent flavonoid glucosyltransferase from Oryza sativa. 水稻尿苷-二磷酸依赖性黄酮类糖基转移酶的研究。

Journal of biochemistry and molecular biology Pub Date : 2007-11-30 DOI: 10.5483/bmbrep.2007.40.6.870

Byoung Seok Hong, Jeong Ho Kim, Na Yeon Kim, Bong-Gyu Kim, Youhoon Chong, Joong-Hoon Ahn

引用次数: 16