Byoung Seok Hong, Jeong Ho Kim, Na Yeon Kim, Bong-Gyu Kim, Youhoon Chong, Joong-Hoon Ahn
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引用次数: 16
摘要
从水稻中克隆了尿苷-二磷酸依赖性糖基转移酶RUGT-10。重组酶通过谷胱甘肽- s转移酶基因融合系统在大肠杆菌中表达。根据底物结构(如黄酮、黄酮醇和黄酮)的不同，RUGT10表现出不同的区域选择性。显然，黄酮如柚皮素和戊二醇得到1个7- o -葡萄糖苷，而黄酮和黄酮醇得到2个以上的产物，其C-3位羟基的糖基化位置优先。
Characterization of uridine-diphosphate dependent flavonoid glucosyltransferase from Oryza sativa.
We cloned a uridine-diphosphate dependent glycosyltransferase RUGT-10 from Oryza sativa. The recombinant enzyme was expressed by glutathione-S transferase gene fusion system in Escherichia coli. RUGT10 showed different regioselectivity depending on the structures of substrates (e.g. flavanone, flavonol, and flavone). Apparently, flavanone such as naringenin and eriodictyol gave one 7-O-glucoside while flavone and flavonol gave more than two products with preferential glucosylation position of hydroxyl group at C-3 position.
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