Journal of biochemistry and molecular biology最新文献_第8页

Site-directed mutagenesis of five conserved residues of subunit i of the cytochrome cbb3 oxidase in Rhodobacter capsulatus. 胞色素cbb3氧化酶i亚基5个保守残基的定点诱变

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.697

Mehmet Ozturk, Ekrem Gurel, Nicholas J Watmough, Sevnur Mandaci

{"title":"Site-directed mutagenesis of five conserved residues of subunit i of the cytochrome cbb3 oxidase in Rhodobacter capsulatus.","authors":"Mehmet Ozturk, Ekrem Gurel, Nicholas J Watmough, Sevnur Mandaci","doi":"10.5483/bmbrep.2007.40.5.697","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.5.697","url":null,"abstract":"Cytochrome cbb(3) oxidase is a member of the heme-copper oxidase superfamily that catalyses the reduction of molecular oxygen to the water and conserves the liberated energy in the form of a proton gradient. Comparison of the amino acid sequences of subunit I from different classes of heme-copper oxidases showed that transmembrane helix VIII and the loop between transmembrane helices IX and X contain five highly conserved polar residues; Ser333, Ser340, Thr350, Asn390 and Thr394. To determine the relationship between these conserved amino acids and the activity and assembly of the cbb(3) oxidase in Rhodobacter capsulatus, each of these five conserved amino acids was substituted for alanine by site-directed mutagenesis. The effects of these mutations on catalytic activity were determined using a NADI plate assay and by measurements of the rate of oxygen consumption. The consequence of these mutations for the structural integrity of the cbb(3) oxidase was determined by SDS-PAGE analysis of chromatophore membranes followed by TMBZ staining. The results indicate that the Asn390Ala mutation led to a complete loss of enzyme activity and that the Ser333Ala mutation decreased the activity significantly. The remaining mutants cause a partial loss of catalytic activity. All of the mutant enzymes, except Asn390Ala, were apparently correctly assembled and stable in the membrane of the R. capsulatus.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 5","pages":"697-707"},"PeriodicalIF":0.0,"publicationDate":"2007-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27040298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Two-component signal transduction in Synechocystis sp. PCC 6803 under phosphate limitation: role of acetyl phosphate. 磷酸限制下聚囊藻(Synechocystis sp. PCC 6803)双组分信号转导:乙酰磷酸的作用。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.708

Waraporn Juntarajumnong, Julian J Eaton-Rye, Aran Incharoensakdi

{"title":"Two-component signal transduction in Synechocystis sp. PCC 6803 under phosphate limitation: role of acetyl phosphate.","authors":"Waraporn Juntarajumnong, Julian J Eaton-Rye, Aran Incharoensakdi","doi":"10.5483/bmbrep.2007.40.5.708","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.5.708","url":null,"abstract":"The two-component signal transduction, which typically consists of a histidine kinase and a response regulator, is used by bacterial cells to sense changes in their environment. Previously, the SphS-SphR histidine kinase and response regulator pair of phosphate sensing signal transduction has been identified in Synechocystis sp. PCC 6803. In addition, some response regulators in bacteria have been shown to be cross regulated by low molecular weight phosphorylated compounds in the absence of the cognate histidine kinase. The ability of an endogenous acetyl phosphate to phosphorylate the response regulator, SphR in the absence of the cognate histidine kinase, SphS was therefore tested in Synechocystis sp. PCC 6803. The mutant lacking functional SphS and acetate kinase showed no detectable alkaline phosphatase activity under phosphate-limiting growth conditions. The results suggested that the endogenous acetyl phosphate accumulated inside the mutants could not activate the SphR via phosphorylation. On the other hand, exogenous acetyl phosphate could allow the mutant lacking functional acetate kinase and phosphotransacetylase to grow under phosphate-limiting conditions suggesting the role of acetyl phosphate as an energy source. Reverse transcription PCR demonstrated that the transcripts of acetate kinase and phosphotransacetylase genes in Synechocystis sp. PCC 6803 is upregulated in response to phosphate limitation suggesting the importance of these two enzymes for energy metabolism in Synechocystis cells.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 5","pages":"708-14"},"PeriodicalIF":0.0,"publicationDate":"2007-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27040299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Removal of the glycosylation of prion protein provokes apoptosis in SF126. 朊蛋白糖基化的去除可引起SF126细胞的凋亡。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.662

Lan Chen, Yang Yang, Jun Han, Bao-Yun Zhang, Lin Zhao, Kai Nie, Xiao-Fan Wang, Feng Li, Chen Gao, Xiao-Ping Dong, Cai-Min Xu

引用次数: 18

Salsolinol, a tetrahydroisoquinoline catechol neurotoxin, induces human Cu,Zn-superoxidie dismutase modificaiton. Salsolinol是一种四氢异喹啉儿茶酚神经毒素，可诱导人Cu, zn -超氧化物歧化酶修饰。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.684

Jung Hoon Kang

引用次数: 10

Biochemical characterization of exoribonuclease encoded by SARS coronavirus. SARS 冠状病毒编码的外切核酸酶的生化特征。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.649

Ping Chen, Miao Jiang, Tao Hu, Qingzhen Liu, Xiaojiang S Chen, Deyin Guo

引用次数: 0

Gene expression profiling of rewarding effect in methamphetamine treated Bax-deficient mouse. 甲基苯丙胺对bax缺陷小鼠奖赏效应的基因表达谱分析。

Journal of biochemistry and molecular biology Pub Date : 2007-07-31 DOI: 10.5483/bmbrep.2007.40.4.475

Na Kyung Ryu, Moon Hee Yang, Min Seok Jung, Jeong Ok Jeon, Kee Won Kim, Jong Hoon Park

{"title":"Gene expression profiling of rewarding effect in methamphetamine treated Bax-deficient mouse.","authors":"Na Kyung Ryu, Moon Hee Yang, Min Seok Jung, Jeong Ok Jeon, Kee Won Kim, Jong Hoon Park","doi":"10.5483/bmbrep.2007.40.4.475","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.4.475","url":null,"abstract":"Methamphetamine is an illicit drug that is often abused and can cause neuropsychiatric and neurotoxic damage. Repeated administration of psychostimulants such as methamphetamine induces a behavioral sensitization. According to a previous study, Bax was involved in neurotoxicity by methamphetamine, but the function of Bax in rewarding effect has not yet been elucidated. Therefore, we have studied the function of Bax in a rewarding effect model. In the present study, we treated chronic methamphetamine exposure in a Bax-deficient mouse model and examined behavioral change using a conditioned place preference (CPP) test. The CPP score in Bax knockout mice was decreased compared to that of wild-type mice. Therefore, we screened for Bax-related genes that are involved in rewarding effect using microarray technology. In order to confirm microarray data, we applied the RT-PCR method to observe relative changes of Bcl2, a pro-apoptotic family gene. As a result, using our experiment microarray, we selected genes that were associated with Bax in microarray data, and eventually selected the Tgfbr2 gene. Expression of the Tgfbr2 gene was decreased by methamphetamine in Bax knockout mice, and the gene was overexpressed in Bax wild-type mice. Additionally, we confirmed that Creb, FosB, and c-Fos were related to rewarding effect and Bax using immunohistochemistry.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 4","pages":"475-85"},"PeriodicalIF":0.0,"publicationDate":"2007-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26863022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Identification of a novel human zinc finger gene, ZNF438, with transcription inhibition activity. 具有转录抑制活性的锌指基因ZNF438的鉴定。

Journal of biochemistry and molecular biology Pub Date : 2007-07-31 DOI: 10.5483/bmbrep.2007.40.4.517

Zhaomin Zhong, Bo Wan, Yun Qiu, Jun Ni, Wenwen Tang, Xinya Chen, Yun Yang, Suqin Shen, Ying Wang, Meirong Bai, Qingyu Lang, Long Yu

{"title":"Identification of a novel human zinc finger gene, ZNF438, with transcription inhibition activity.","authors":"Zhaomin Zhong, Bo Wan, Yun Qiu, Jun Ni, Wenwen Tang, Xinya Chen, Yun Yang, Suqin Shen, Ying Wang, Meirong Bai, Qingyu Lang, Long Yu","doi":"10.5483/bmbrep.2007.40.4.517","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.4.517","url":null,"abstract":"There were many different families of zinc finger proteins that contained multiple cysteine and/or histidine residues and used zinc to stabilize their folds. The classical C2H2 zinc finger proteins were the founding members of this superfamily and were among the most abundant proteins in eukaryotic genomes. C2H2 proteins typically contained several C2H2 fingers that made tandem contacts along the DNA. Here we reported a novel C2H2 type zinc finger gene, ZNF438, which encoded 828 amino acids that formed five zinc finger domains. Bioinformatics analysis revealed that the ZNF438 was mapped to human chromosome 10p11.2 and shared 62% identity with rat and mouse homologues. RT-PCR analysis indicated that it was ubiquitously expressed in 18 human adult tissues. With immunofluorescence assay, it was shown that the exogenous Flag-tagged ZNF438 was located in nucleus of COS-7 cells. To further explore the function of ZNF438, we examined the transcriptional activity of ZNF438 protein by transfecting recombinant pM-ZNF438 into mammalian cells. The subsequent analysis based on the duel luciferase assay system showed that ZNF438 was a transcriptional repressor.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 4","pages":"517-24"},"PeriodicalIF":0.0,"publicationDate":"2007-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26863027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Cloning of phospholipase D from grape berry and its expression under heat acclimation. 葡萄果实磷脂酶D的克隆及其热驯化条件下的表达。

Journal of biochemistry and molecular biology Pub Date : 2007-07-31 DOI: 10.5483/bmbrep.2007.40.4.595

Si-Bao Wan, Wei Wang, Peng-Fei Wen, Jian-Ye Chen, Wei-Fu Kong, Qiu-Hong Pan, Ji-Cheng Zhan, Li Tian, Hong-Tao Liu, Wei-Dong Huang

{"title":"Cloning of phospholipase D from grape berry and its expression under heat acclimation.","authors":"Si-Bao Wan, Wei Wang, Peng-Fei Wen, Jian-Ye Chen, Wei-Fu Kong, Qiu-Hong Pan, Ji-Cheng Zhan, Li Tian, Hong-Tao Liu, Wei-Dong Huang","doi":"10.5483/bmbrep.2007.40.4.595","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.4.595","url":null,"abstract":"To investigate whether phospholipase D (PLD, EC 3.1.4.4) plays a role in adaptive response of post-harvest fruit to environment, a PLD gene was firstly cloned from grape berry (Vitis Vinifera L. cv. Chardonnay) using RT-PCR and 3'- and 5'-RACE. The deduced amino acid sequence (809 residues) showed 84.7% identity with that of PLD from Ricinus communis. The secondary structures of this protein showed the characteristic C2 domain and two active sites of a phospholipid-metabolizing enzyme. The PLD activity and its expression in response to heat acclimation were then assayed. The results indicated PLD was significantly activated at enzyme activity, as well as accumulation of PLD mRNA and synthesis of new PLD protein during the early of heat acclimation, primary suggesting that the grape berry PLD may be involved in the heat response in post-harvest grape berry. This work offers an important basis for further investigating the mechanism of post-harvest fruit adaptation to environmental stresses.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 4","pages":"595-603"},"PeriodicalIF":0.0,"publicationDate":"2007-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26864174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Effect of dendritic cells treated with CpG ODN on atopic dermatitis of Nc/Nga mice. CpG ODN处理树突状细胞对Nc/Nga小鼠特应性皮炎的影响。

Journal of biochemistry and molecular biology Pub Date : 2007-07-31 DOI: 10.5483/bmbrep.2007.40.4.486

Sang-Tae Park, Kyoung-Eun Kim, Kwangmin Na, Younghwa Kim, Tae-Yoon Kim

{"title":"Effect of dendritic cells treated with CpG ODN on atopic dermatitis of Nc/Nga mice.","authors":"Sang-Tae Park, Kyoung-Eun Kim, Kwangmin Na, Younghwa Kim, Tae-Yoon Kim","doi":"10.5483/bmbrep.2007.40.4.486","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.4.486","url":null,"abstract":"Atopic dermatitis (AD) is a chronic inflammatory skin disease and the pathogenesis of AD is associated with the release of various cytokines/chemokines due to activated Th(2) immune responses. Synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG dinucleotide in the context of particular base sequence (CpG motifs) are known to have the immunostimulatory activities in mice and to convert from Th(2) to Th(1) immune responses in AD. We aimed to investigate that CpG ODN, especially phosphodiester form, can stimulate the protective immunity in NC/Nga mice with AD. We isolated BMDCs from NC/Nga mice and then, cultured with GM-CSF and IL-4 for 6 days, and treated for 2 days by either phosphorothioate ODN or phosphodiester ODN. CpG ODN-treated DCs resulted in more production of IL-12. When CpG ODN-treated DCs were intravenously injected into the NC/Nga mice, the NC/Nga mice with CpG ODN-treated DCs showed significant improvement of AD symptoms and decrease of IgE level. Histopathologically, the NC/Nga mice skin with CpG ODN-treated DCs showed the decreased IL-4 and TARC expression comparing with non-injected mice. These results may suggest that phosphodiester CpG ODN-treated DCs might function as a potent adjuvant for AD in a mouse model.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 4","pages":"486-93"},"PeriodicalIF":0.0,"publicationDate":"2007-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26863023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Expressed sequence tag analysis for identification and characterization of sex-related genes in the giant tiger shrimp Penaeus monodon. 单口对虾性别相关基因的表达序列标记分析。

Journal of biochemistry and molecular biology Pub Date : 2007-07-31 DOI: 10.5483/bmbrep.2007.40.4.501

Rachanimuk Preechaphol, Rungnapa Leelatanawit, Kanchana Sittikankeaw, Sirawut Klinbunga, Bavornlak Khamnamtong, Narongsak Puanglarp, Piamsak Menasveta

{"title":"Expressed sequence tag analysis for identification and characterization of sex-related genes in the giant tiger shrimp Penaeus monodon.","authors":"Rachanimuk Preechaphol, Rungnapa Leelatanawit, Kanchana Sittikankeaw, Sirawut Klinbunga, Bavornlak Khamnamtong, Narongsak Puanglarp, Piamsak Menasveta","doi":"10.5483/bmbrep.2007.40.4.501","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.4.501","url":null,"abstract":"Sex-related genes expressed in vitellogenic ovaries of the giant tiger shrimp, Penaeus monodon, were identified by an EST approach. A total of 1051 clones were unidirectionally sequenced from the 5 terminus. Nucleotide sequences of 743 EST (70.7%) significantly matched known genes previously deposited in the GenBank (E-value <10(-4)) whereas 308 ESTs (29.3%) were regarded as newly unidentified transcripts (E-value >10(-4)). A total of 559 transcripts (87 contigs and 472 singletons) were obtained. Thrombospondin (TSP) and peritrophin (79 and 87 clones accounting for 7.5 and 8.3% of clones sequenced, respectively) predominated among characterized transcripts. Several full length transcripts (e.g. cyclophilin, profillin and thioredoxin peroxidase) were also isolated. A gene homologue encoding chromobox protein (PMCBX, ORF of 567 nucleotides encoding a protein of 188 amino acids) which is recognized as a new member of the HP1 family was identified. Expression patterns of 14 of 25 sex-related gene homologues in ovaries and testes of P. monodon broodstock were examined by RT-PCR. Female sterile and ovarian lipoprotein receptor homologues were only expressed in ovaries whereas the remaining transcripts except disulfide isomerase related P5 precursor and adenine nucleotide translocator 2 were higher expressed in ovaries than testes of P. monodon broodstock. A homologue of ubiquitin specific proteinase 9, X chromosome (Usp9X) revealed a preferential expression level in ovaries than testes of broodstock-sized P. monodon (N = 13 and 11, P <0.05) but was only expressed in ovaries of 4-month-old shrimp (N = 5 for each sex).","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 4","pages":"501-10"},"PeriodicalIF":0.0,"publicationDate":"2007-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26863025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 79