Journal of biochemistry and molecular biology最新文献_第6页

Molecular cloning and characterization of the yew gene encoding squalene synthase from Taxus cuspidata. 东北红豆杉角鲨烯合成酶基因的克隆与鉴定。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.625

Zhuosh Huang, Keji Jiang, Yan Pi, Rong Hou, Zhihua Liao, Ying Cao, Xu Han, Qian Wang, Xiaofen Sun, Kexuan Tang

{"title":"Molecular cloning and characterization of the yew gene encoding squalene synthase from Taxus cuspidata.","authors":"Zhuosh Huang, Keji Jiang, Yan Pi, Rong Hou, Zhihua Liao, Ying Cao, Xu Han, Qian Wang, Xiaofen Sun, Kexuan Tang","doi":"10.5483/bmbrep.2007.40.5.625","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.5.625","url":null,"abstract":"The enzyme squalene synthase (EC 2.5.1.21) catalyzes a reductive dimerization of two farnesyl diphosphate (FPP) molecules into squalene, a key precursor for the sterol and triterpene biosynthesis. A full-length cDNA encoding squalene synthase (designated as TcSqS) was isolated from Taxus cuspidata, a kind of important medicinal plants producing potent anti-cancer drug, taxol. The full-length cDNA of TcSqS was 1765 bp and contained a 1230 bp open reading frame (ORF) encoding a polypeptide of 409 amino acids. Bioinformatic analysis revealed that the deduced TcSqS protein had high similarity with other plant squalene synthases and a predicted crystal structure similar to other class I isoprenoid biosynthetic enzymes. Southern blot analysis revealed that there was one copy of TcSqS gene in the genome of T. cuspidata. Semiquantitative RT-PCR analysis and northern blotting analysis showed that TcSqS expressed constitutively in all tested tissues, with the highest expression in roots. The promoter region of TcSqS was also isolated by genomic walking and analysis showed that several cis-acting elements were present in the promoter region. The results of treatment experiments by different signaling components including methyl-jasmonate, salicylic acid and gibberellin revealed that the TcSqS expression level of treated cells had a prominent diversity to that of control, which was consistent with the prediction results of TcSqS promoter region in the PlantCARE database.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 5","pages":"625-35"},"PeriodicalIF":0.0,"publicationDate":"2007-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27040472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 40

Macrophage activation by an acidic polysaccharide isolated from Angelica sinensis (Oliv.) Diels. 当归酸性多糖对巨噬细胞的激活作用一昼夜的。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.636

Xingbin Yang, Yan Zhao, Haifang Wang, Qibing Mei

引用次数: 54

Inhibitory effect of ginkgolide B on platelet aggregation in a cAMP- and cGMP-dependent manner by activated MMP-9. 银杏内酯B通过激活MMP-9以cAMP-和cmpp依赖的方式抑制血小板聚集。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.678

Hyun-Jeong Cho, Kyung-Soo Nam

{"title":"Inhibitory effect of ginkgolide B on platelet aggregation in a cAMP- and cGMP-dependent manner by activated MMP-9.","authors":"Hyun-Jeong Cho, Kyung-Soo Nam","doi":"10.5483/bmbrep.2007.40.5.678","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.5.678","url":null,"abstract":"Extracts from the leaves of the Ginkgo biloba are becoming increasingly popular as a treatment that is claimed to reduce atherosclerosis, coronary artery disease, and thrombosis. In this study, the effect of ginkgolide B (GB) from Ginkgo biloba leaves in collagen (10 microg/ml)- stimulated platelet aggregation was investigated. It has been known that human platelets release matrix metalloproteinase- 9 (MMP-9), and that it significantly inhibited platelet aggregation stimulated by collagen. Zymographic analysis confirmed that pro-MMP-9 (92-kDa) was activated by GB to form an MMP-9 (86-kDa) on gelatinolytic activities. And then, activated MMP-9 by GB dose-dependently inhibited platelet aggregation, intracellular Ca2+ mobilization, and thromboxane A2 (TXA2) formation in collagen-stimulated platelets. Activated MMP-9 by GB directly affects down-regulations of cyclooxygenase-1 (COX-1) or TXA2 synthase in a cell free system. In addition, activated MMP-9 significantly increased the formation of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), which have the anti-platelet function in resting and collagen-stimulated platelets. Therefore, we suggest that activated MMP-9 by GB may increase the intracellular cAMP and cGMP production, inhibit the intracellular Ca2+ mobilization and TXA2 production, thereby leading to inhibition of platelet aggregation. These results strongly indicate that activated MMP-9 is a potent inhibitor of collagen-stimulated platelet aggregation. It may act a crucial role as a negative regulator during platelet activation.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 5","pages":"678-83"},"PeriodicalIF":0.0,"publicationDate":"2007-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27041452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Molecular characterization of a transient expression gene encoding for 1-aminocyclopropane-1-carboxylate synthase in cotton (Gossypium hirsutum L.). 棉花1-氨基环丙烷-1-羧酸合成酶瞬时表达基因的分子特征。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.791

Xia Wang, Ying Zhang, Jiedao Zhang, Cheng Cheng, Xingqi Guo

{"title":"Molecular characterization of a transient expression gene encoding for 1-aminocyclopropane-1-carboxylate synthase in cotton (Gossypium hirsutum L.).","authors":"Xia Wang, Ying Zhang, Jiedao Zhang, Cheng Cheng, Xingqi Guo","doi":"10.5483/bmbrep.2007.40.5.791","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.5.791","url":null,"abstract":"Ethylene performs an important function in plant growth and development. 1-aminocyclopropane-1-carboxylate (ACC) synthase (ACS), the key enzyme involved in ethylene biosynthesis, has been the focus of most ethylene studies. Here, a cotton ACS gene referred to as Gossypium hirsutum ACS1 (GhACS1), was isolated. The full-length cDNA of GhACS1 encodes for a 476-amino acid protein which harbors seven conserved regions, 11 invariant amino acid residues, and the PLP binding active site, all of which characterize ACC synthases. Alignment analysis showed that GhACS1 shared a high degree of identity with other known ACC synthases from different species. Two introns were detected in the genomic DNA sequence, and the results of Southern blot analysis suggested that there might be a multi-gene family encoding for ACC synthase in cotton. From the phylogenetic tree constructed with 24 different kinds of ACC synthases, we determined that GhACS1 falls into group II, and was closely associated with the wound-inducible ACS of citrus. The analysis of the 5' flanking region of GhACS1 revealed a group of putative cis-acting elements. The results of expression analysis showed that GhACS1 displayed its transient expression nature after wounding, abscisic acid (ABA), and CuCl(2) treatments. These results indicate that GhACS1, which was transiently expressed in response to certain stimuli, may be involved in the production of ethylene for the transmission of stress signals.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 5","pages":"791-800"},"PeriodicalIF":0.0,"publicationDate":"2007-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27041845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Type I collagen-induced pro-MMP-2 activation is differentially regulated by H-Ras and N-Ras in human breast epithelial cells. 在人乳腺上皮细胞中，I型胶原诱导的前mmp -2激活受H-Ras和N-Ras的差异调节。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.825

In Young Kim, Seo-Jin Jeong, Eun-Sook Kim, Seung Hee Kim, Aree Moon

{"title":"Type I collagen-induced pro-MMP-2 activation is differentially regulated by H-Ras and N-Ras in human breast epithelial cells.","authors":"In Young Kim, Seo-Jin Jeong, Eun-Sook Kim, Seung Hee Kim, Aree Moon","doi":"10.5483/bmbrep.2007.40.5.825","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.5.825","url":null,"abstract":"Tumor cell invasion and metastasis are often associated with matrix metalloproteinases (MMPs), among which MMP-2 and MMP-9 are of central importance. We previously showed that H-Ras, but not N-Ras, induced invasion of MCF10A human breast epithelial cells in which the enhanced expression of MMP-2 was involved. MMP-2 is produced as a latent pro-MMP-2 (72 kDa) to be activated resulting the 62 kDa active MMP-2. The present study investigated if H-Ras and/or N-Ras induces pro-MMP-2 activation of MCF10A cells when cultured in two-dimensional gel of type I collagen. Type I collagen induced activation of pro-MMP-2 only in H-Ras MCF10A cells but not in N-Ras MCF10A cells. Induction of active MMP-2 by type I collagen was suppressed by blocking integrin alpha2, indicating the involvement of integrin signaling in pro-MMP-2 activation. Membrane-type (MT)1-MMP and tissue inhibitor of metalloproteinase (TIMP)-2 were up-regulated by H-Ras but not by N-Ras in the type I collagen-coated gel, suggesting that H-Ras-specific up-regulation of MT1-MMP and TIMP-2 may lead to the activation of pro-MMP-2. Since acquisition of pro-MMP-2 activation can be associated with increased malignant progression, these results may help understanding the mechanisms for the cell surface matrix-degrading potential which will be crucial to the prognosis and therapy of breast cancer metastasis.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 5","pages":"825-31"},"PeriodicalIF":0.0,"publicationDate":"2007-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27038478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Alteration of the quaternary structure of human UDP-glucose dehydrogenase by a double mutation. 双突变对人udp -葡萄糖脱氢酶四级结构的影响。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.690

Jae-Wan Huh, Seung-Ju Yang, Eun Young Hwang, Myung-Min Choi, Hyun-Ju Lee, Eun-A Kim, Soo Young Choi, Jene Choi, Hea-Nam Hong, Sung-Woo Cho

引用次数: 8

Characterization of aldolase from Methanococcus jannaschii by gas chromatography. 气相色谱法表征jannaschii甲烷球菌醛缩酶。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.801

Jeong E Nam Shin, Mi-Jung Kim, Ji-ah Choi, Keun Ho Chun

引用次数: 0

Molecular cloning and characterization of a new cDNA encoding hyoscyamine 6beta-hydroxylase from roots of Anisodus acutangulus. 山莨菪根山莨菪胺6 -羟化酶cDNA的克隆与鉴定

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.715

Guoyin Kai, Junfeng Chen, Li Li, Genyu Zhou, Limin Zhou, Lei Zhang, Yuhui Chen, Linxia Zhao

{"title":"Molecular cloning and characterization of a new cDNA encoding hyoscyamine 6beta-hydroxylase from roots of Anisodus acutangulus.","authors":"Guoyin Kai, Junfeng Chen, Li Li, Genyu Zhou, Limin Zhou, Lei Zhang, Yuhui Chen, Linxia Zhao","doi":"10.5483/bmbrep.2007.40.5.715","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.5.715","url":null,"abstract":"A new full-length cDNA encoding hyoscyamine 6beta-hydroxylase (designated as aah6h, GenBank Accession No. EF187826), which catalyzes the last committed step in the scopolamine biosynthetic pathway, was isolated from young roots of Anisodus acutangulus by rapid amplification of cDNA ends (RACE) for the first time. The full-length cDNA of aah6h was 1380 bp and contained a 1035 bp open reading frame (ORF) encoding a deduced protein of 344 amino acid residues. The deduced protein had an isoelectric point (pI) of 5.09 and a calculated molecular mass of about 38.7 kDa. Sequence analyses showed that AaH6H had high homology with other H6Hs isolated from some scopolamine-producing plants such as Hyoscyamus niger, Datura metel and Atropa belladonna etc. Bioinformatics analyses results indicated AaH6H belongs to 2-oxoglutarate-dependent dioxygenase superfamily. Phylogenetic tree analysis showed that AaH6H had closest relationship with H6H from A. tanguticus. Southern hybridization analysis of the genomic DNA revealed that aah6h belonged to a multi-copy gene family. Tissue expression pattern analysis firstly founded that aah6h expressed in all the tested tissues including roots, stems and leaves and indicated that aah6h was a constitutive-expression gene, which was the first reported tissue-independent h6h gene compared to other known h6h genes.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 5","pages":"715-22"},"PeriodicalIF":0.0,"publicationDate":"2007-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27040300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 37

Isolation and characterization of mouse testis specific serine/threonine kinase 5 possessing four alternatively spliced variants. 小鼠睾丸特异性丝氨酸/苏氨酸激酶5的分离与表征，该激酶具有四种选择性剪接变体。

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.749

Youheng Wei, Guolong Fu, Hairong Hu, Gang Lin, Jingchun Yang, Jinhu Guo, Qiquan Zhu, Long Yu

{"title":"Isolation and characterization of mouse testis specific serine/threonine kinase 5 possessing four alternatively spliced variants.","authors":"Youheng Wei, Guolong Fu, Hairong Hu, Gang Lin, Jingchun Yang, Jinhu Guo, Qiquan Zhu, Long Yu","doi":"10.5483/bmbrep.2007.40.5.749","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.5.749","url":null,"abstract":"Phosphorylation on serine/threonine or tyrosine residues of target proteins is an essential and significant regulatory mechanism in signal transduction during many cellular and life processes, including spermatogenesis, oogenesis and fertilization. In the present work, we reported the isolation and characterization of mouse testis-specific serine/threonine kinase 5 (Tssk5), which contains four alternatively spliced variants including, Tssk5alpha, Tssk5beta, Tssk5gamma and Tssk5delta. Moreover, the locus of Tssk5 is on chromosome 14qC3 and the four variants had a similar high expression in the testis and the heart; however, had a low expression in other tissues, except for Tssk5alpha which also had comparably high expression in the spleen. Each variant of Tssk5 expression began in the testis 16 days after birth. Aside from TSSK5alpha, the other isoforms have an insertion of ten amino acid residues (RLTPSLSAAG) in region VIb (HRD domain) (His-Arg-Asp). Moreover, only TSSK5alpha exhibited kinase activity and consistently, a further Luciferase Reporter Assay demonstrated that TSSK5beta, TSSK5gamma and TSSK5delta cannot be stimulated at the CREB/CRE responsive pathway in cmparison to TSSK5alpha. These findings suggest that TSSK5beta, TSSK5gamma, TSSK5delta may be pseudokinases due to the insertion, which may damage the structure responsible for active kinase activity. Pull-down assay experiments indicated that TSSK5beta, TSSK5gamma and TSSK5delta can directly interact with TSSK5alpha. In summary, these four isoforms with similar expression patterns may be involved in spermatogenesis through a coordinative way in testis.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 5","pages":"749-56"},"PeriodicalIF":0.0,"publicationDate":"2007-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27040304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

A novel role of classical swine fever virus E(rns) glycoprotein in counteracting the newcastle disease virus (NDV)-mediated IFN-beta Induction. 经典猪瘟病毒E(rns)糖蛋白在对抗新城疫病毒(NDV)介导的ifn - β诱导中的新作用

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI: 10.5483/bmbrep.2007.40.5.611

Yan-hua Xia, Liu Chen, Zi-shu Pan, Chu-yu Zhang

{"title":"A novel role of classical swine fever virus E(rns) glycoprotein in counteracting the newcastle disease virus (NDV)-mediated IFN-beta Induction.","authors":"Yan-hua Xia, Liu Chen, Zi-shu Pan, Chu-yu Zhang","doi":"10.5483/bmbrep.2007.40.5.611","DOIUrl":"https://doi.org/10.5483/bmbrep.2007.40.5.611","url":null,"abstract":"E(rns) is an envelope glycoprotein of classical swine fever virus (CSFV) and has an unusual feature of RNase activity. In the present study, we demonstrate that E(rns) counteracts Newcastle disease virus (NDV)-mediated induction of IFN-beta. For this purpose, E(rns) fused to the enhanced green fluorescent protein (EGFP) was transiently expressed in porcine kidney 15 (PK15) cells. In luciferase activity assay, E(rns)-EGFP was found to prevent IFN-beta promoter-driven luciferase expression and block the induction of IFN-beta promoter mediated by NDV in a dosedependent manner. Through IFN-specific semi-quantitative RT-PCR detection, obvious decrease of IFN-beta mRNA in NDV-infected PK15 cells was observed in the presence of E(rns)-EGFP. In contrast, EGFP alone showed none of this block capacity. In addition, E(rns)-EGFP mutations with RNase inactivation were also found to block NDV-mediated induction of IFN-beta. These evidences establish a novel function for CSFV E(rns) glycoprotein in counteraction of the IFN-beta induction pathway.","PeriodicalId":15113,"journal":{"name":"Journal of biochemistry and molecular biology","volume":"40 5","pages":"611-6"},"PeriodicalIF":0.0,"publicationDate":"2007-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27040470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15