Journal of Bacteriology最新文献

{"title":"Unraveling the gut microbiota's role in obesity: key metabolites, microbial species, and therapeutic insights.","authors":"Majid Iqbal, Qian Yu, Jingqun Tang, Juanjuan Xiang","doi":"10.1128/jb.00479-24","DOIUrl":"https://doi.org/10.1128/jb.00479-24","url":null,"abstract":"<p><p>Obesity, characterized by excessive fat accumulation, stems from an imbalance between energy intake and expenditure, with the gut microbiota playing a crucial role. This review highlights how gut microbiota influences metabolic pathways, inflammation, and adipose tissue regulation in obesity. Specific bacteria and metabolites, such as lipopolysaccharides (LPS) and short-chain fatty acids (SCFAs), modulate gut permeability, inflammation, and energy harvest, impacting obesity development. Certain gut bacteria, including <i>Clostridium XIVb</i>, <i>Dorea</i> spp., <i>Enterobacter cloacae</i>, and <i>Collinsella aerofaciens</i>, promote obesity by increasing energy harvest, gut permeability, and inflammatory response through LPS translocation into the bloodstream. Conversely, beneficial bacteria like <i>Akkermansia muciniphila</i>, <i>Lactobacillus</i> spp., and <i>Bifidobacterium</i> spp. enhance gut barrier integrity, regulate SCFA production, and modulate fasting-induced adipose factor, which collectively support metabolic health by reducing fat storage and inflammation. Metabolites such as SCFAs (acetate, propionate, and butyrate) interact with G-protein coupled receptors to regulate lipid metabolism and promote the browning of white adipose tissue (WAT), thus enhancing thermogenesis and energy expenditure. However, LPS contributes to insulin resistance and fat accumulation, highlighting the dual roles of these microbial metabolites in both supporting and disrupting metabolic function. Therapeutic interventions targeting gut microbiota, such as promoting WAT browning and activating brown adipose tissue (BAT), hold promise for obesity management. However, personalized approaches are necessary due to individual microbiome variability. Further research is essential to translate these insights into microbiota-based clinical therapies.</p>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":" ","pages":"e0047924"},"PeriodicalIF":2.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid screening and identification of genes involved in bacterial extracellular membrane vesicle production using a curvature-sensing peptide.","authors":"Hiromu Inoue, Kenichi Kawano, Jun Kawamoto, Takuya Ogawa, Tatsuo Kurihara","doi":"10.1128/jb.00497-24","DOIUrl":"https://doi.org/10.1128/jb.00497-24","url":null,"abstract":"<p><p>Bacteria secrete extracellular membrane vesicles (EMVs). Physiological functions and biotechnological applications of these lipid nanoparticles have been attracting significant attention. However, the details of the molecular basis of EMV biogenesis have not yet been fully elucidated. In our previous work, an N-terminus-substituted FAAV peptide labeled with nitrobenzoxadiazole (NBD; nFAAV5-NBD) was developed. This peptide can sense the curvature of a lipid bilayer and selectively bind to EMVs even in the presence of cells. Here, we applied nFAAV5-NBD to a genome-wide screening of hyper- and hypo-vesiculation transposon mutants of a Gram-negative bacterium, <i>Shewanella vesiculosa</i> HM13, to identify the genes involved in EMV production. We analyzed the transposon insertion sites in hyper- and hypo-vesiculation mutants and identified 16 and six genes, respectively, with a transposon inserted within or near them. Targeted gene-disrupted mutants of the identified genes showed that the lack of putative dipeptidyl carboxypeptidase, glutamate synthase β-subunit, LapG protease, metallohydrolase, RNA polymerase sigma-54 factor, inactive transglutaminase, PepSY domain-containing protein, and Rhs-family protein caused EMV overproduction. On the other hand, disruption of the genes encoding putative phosphoenolpyruvate synthase, d-hexose-6-phosphate epimerase, NAD-specific glutamate dehydrogenase, and sensory box histidine kinase/response regulator decreased EMV production. This study demonstrates the utility of a novel screening method using a curvature-sensing peptide for mutants with altered EMV productivity and provides information on the genes related to EMV production.IMPORTANCEConventional methods for isolation and quantification of extracellular membrane vesicles (EMVs) are generally time-consuming. nFAAV5-NBD can detect EMVs in the culture without separating EMVs from cells. <i>In situ</i> detection of EMVs using this peptide facilitated screening of the genes related to EMV production. We succeeded in identifying various genes associated with EMV production of <i>Shewanella vesiculosa</i> HM13, which would contribute to the elucidation of bacterial EMV formation mechanisms. Additionally, the hyper-vesiculating mutants obtained in this study would be valuable for EMV applications, such as secreting useful substances as EMV cargoes and producing artificially functionalized EMVs.</p>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":" ","pages":"e0049724"},"PeriodicalIF":2.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Bacillus subtilis</i> MurJ and Amj Lipid II flippases are not essential for growth.","authors":"Kiera Englehart, Jonathan Dworkin","doi":"10.1128/jb.00078-25","DOIUrl":"https://doi.org/10.1128/jb.00078-25","url":null,"abstract":"<p><p>Identification of the protein that mediates transbilayer transport of the undecaprenyl-pyrophosphate (Und-PP) linked peptidoglycan precursor Lipid II has long been a subject of investigation. Candidates belonging to both the MOP (multidrug/oligosaccharidyl-lipid/polysaccharide) and SEDS (shape, elongation, division and sporulation) families of transmembrane proteins have been proposed, exhibiting characteristics consistent with mediating this process, including genetic essentiality and biochemical activity. While MOP family proteins including MurJ are widely considered to be the primary Lipid II transporter, questions still remain including a role for the SEDS proteins in this process. We and others previously showed that a <i>Bacillus subtilis</i> strain lacking all four MurJ homologs is viable, thereby implicating a separate mode of Lipid II transport across the membrane. However, a subsequent report of synthetic essentiality between <i>B. subtilis</i> MurJ and the flippase Amj suggested that they are necessary and sufficient. Here, we show that this effect is alleviated by excess synthesis of the enzyme responsible for Und-PP production. Thus, the inviability of a <i>murJ-amj</i> double mutant strain is not due to the essentiality of these enzymes for flipping Lipid II but is instead most likely a consequence of a reduction of free Und-PP levels. This result is consistent with a non-MOP-dependent pathway for Lipid II transport across the cytoplasmic membrane to enable cell wall peptidoglycan synthesis.IMPORTANCEThe assembly of peptidoglycan (PG), the typically essential polymer that provides structural integrity to bacterial cells, begins with the synthesis of the Lipid II monomer in the cytoplasm and along the cytoplasmic face of the inner membrane. Lipid II is then translocated across the membrane to the extracellular site of polymerization. The mechanistic basis for this process remains unclear, with genetic and/or biochemical evidence pointing to two different families of conserved membrane proteins. Here, we present genetic evidence that only one of these two families is essential in <i>Bacillus subtilis</i>.</p>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":" ","pages":"e0007825"},"PeriodicalIF":2.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial peptide plectasin recombinantly produced in <i>Escherichia coli</i> disintegrates cell walls of gram-positive bacteria, as proven by transmission electron and atomic force microscopy.","authors":"Matthias Müller, Sigrid Mayrhofer, Wisnu Arfian A Sudjarwo, Martin Gibisch, Christopher Tauer, Eva Berger, Cécile Brocard, José L Toca-Herrera, Gerald Striedner, Rainer Hahn, Monika Cserjan-Puschmann","doi":"10.1128/jb.00456-24","DOIUrl":"https://doi.org/10.1128/jb.00456-24","url":null,"abstract":"<p><p>Plectasin, an antimicrobial peptide, was initially isolated from the saprophytic fungus <i>Pseudoplectania nigrella</i>. This peptide, a member of the cysteine-stabilized α-helix and β-sheet family, has demonstrated potent antimicrobial activity against gram-positive pathogens, including strains resistant to conventional antibiotics. Our CASPON platform process enables the production of substantial quantities of plectasin, facilitating investigations on the activity and the mode of action of this recombinantly produced peptide. To this end, we developed an activity assay that reflects the growth inhibition of selected model bacteria, allowing for statistical analysis and evaluation of reproducibility. The mode of action was investigated using transmission electron microscopy and atomic force microscopy. The latter provided new insights into alterations in the cell surface of gram-positive bacteria treated with plectasin at the single-cell level. While the cell diameter remained unaltered, the roughness increased by up to twofold, and the cell stiffness decreased by approximately one-third in the four gram-positive bacterial strains tested. Statistical analysis of these morphological changes provides further insights into the effects and efficiency of antimicrobial peptides targeting pathogen cell walls.</p><p><strong>Importance: </strong>The rise of antibiotic-resistant bacteria is a major threat to global health. Antimicrobial peptides (AMPs) offer a promising way to combat this. With the CASPON technology, we produced the AMP plectasin comprising three disulfide bonds using <i>Escherichia coli</i>. The activity of purified plectasin with and without a CASPON fusion tag was determined for four gram-positive and four gram-negative bacteria. As anticipated, only gram-positive bacteria showed a growth inhibition response to un-tagged plectasin. Plectasin treatment on gram-positive bacteria was visualized via electron microscopy. Evaluation of atomic force microscopy indicated that plectasin treatment led to increased roughness but maintained thickness. Based on our study, we assume that the CASPON technology can be employed in the future for the production and characterization of medical-grade AMPs.</p>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":" ","pages":"e0045624"},"PeriodicalIF":2.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlled burn: interconnections between energy-spilling pathways and metabolic signaling in bacteria.","authors":"Nicolaus Jakowec, Steven E Finkel","doi":"10.1128/jb.00542-24","DOIUrl":"https://doi.org/10.1128/jb.00542-24","url":null,"abstract":"<p><p>Bacterial energy-spilling pathways-such as overflow metabolism and futile cycles-have been considered inefficient forms of metabolism that result from poor regulatory control or function as mechanisms to cope with excess energy. However, mounting evidence places these seemingly wasteful reactions at the fulcrum between metabolic signaling and stress adaptation in bacteria. Specifically, energy-spilling pathways may mediate the metabolic reprogramming observed when cells encounter growth-limiting constraints (i.e., nutrient limitation). Recent insights spotlight microbial metabolism as an intricate signaling network that coordinates physiological programming with energy and nutrient conditions. Such intracellular metabolic cross stalk is pivotal to survival in competitive, fluctuating environments that bacteria frequently encounter in nature. In light of this paradigm of metabolic signaling, energy-spilling pathways are increasingly recognized as regulatory strategies that enable metabolic rewiring in response to stress. Overflow metabolism or futile cycles may generate secondary metabolites with signaling properties, alter the flux of metabolic pathways and the rate of nutrient acquisition, or stimulate regulatory nodes to trigger specific metabolic programs in response to environmental challenges. Furthermore, the observation of such expensive pathways under laboratory conditions purported to be \"energy limiting\" may in fact suggest energy sufficiency, compelling us to rethink how we model energy limitation and starvation for bacteria.</p>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":" ","pages":"e0054224"},"PeriodicalIF":2.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond anaerobic respiration-new physiological roles for DmsABC and other S-/N-oxide reductases in <i>Escherichia coli</i>.","authors":"Qifeng Zhong, Marufa Nasreen, Ruizhe Yang, Michel Struwe, Bostjan Kobe, Ulrike Kappler","doi":"10.1128/jb.00463-24","DOIUrl":"https://doi.org/10.1128/jb.00463-24","url":null,"abstract":"<p><p>Sulfoxide reductases in pathogenic bacteria have recently received increasing attention for their association with virulence and survival within the host. Here, we have re-investigated the physiological role of the molybdenum-containing DmsABC dimethyl sulfoxide (DMSO) reductase from <i>Escherichia coli</i>, which has a proposed role in anaerobic respiration with DMSO. Our investigation into potential physiological substrates revealed that DmsABC efficiently reduces pyrimidine N-oxide, nicotinamide N-oxide, and methionine sulfoxide, and exposure to host cell-produced stressors such as hypochlorite or hydrogen peroxide specifically increased expression of the <i>E. coli dmsA</i> gene. <i>E. coli</i> strains lacking <i>dmsA</i> showed increased lag times in the presence of hypochlorite, and these strains also showed up to a 90% reduction in adherence to human bladder cells. Interestingly, in the presence of hypochlorite, expression of multiple alternative S-/N-oxide reductases present in <i>E. coli</i> was elevated by 2- to 4-fold in a ∆<i>dmsA</i> strain compared to the wild-type strain, suggesting functional redundancy. The phenotypes of the <i>E. coli</i> ∆<i>dmsA</i> strains were strikingly similar to ∆<i>dmsA</i> strains of the respiratory pathogen <i>Haemophilus influenzae</i>, which confirms the role of both enzymes in supporting host-pathogen interactions. We propose that this function is conserved in enzymes closely related to <i>E. coli</i> DmsABC. Our study also uncovered that the expression of many <i>E. coli</i> Mo enzymes was induced by oxidative stressors, including metals such as copper, and further work should be directed at determining the connection of these enzymes to host-pathogen interactions.IMPORTANCEBacterial urinary tract infections are debilitating and frequently recurring in human populations worldwide, and <i>Escherichia coli</i> strains are a major cause of these infections. In this study, we have uncovered a new mechanism by which <i>E. coli</i> can avoid being killed by the human immune system. The bacteria use a set of seven related enzymes that can reverse damage to essential cell components such as amino acids, vitamins, and DNA building blocks. Antibacterial compounds produced by the human immune system specifically induced the production of these enzymes, confirming that they play a role in helping <i>E. coli</i> survive during infection and making these enzymes potential future drug targets.</p>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":" ","pages":"e0046324"},"PeriodicalIF":2.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SloR-SRE binding to the <i>S. mutans mntH</i> promoter is cooperative.","authors":"Myrto Ziogas, India Drummond, Igor Todorovic, Katie Kraczkowsky, Yiran Han, Hua Zhang, Hui Wu, Grace Spatafora","doi":"10.1128/jb.00470-24","DOIUrl":"https://doi.org/10.1128/jb.00470-24","url":null,"abstract":"<p><p><i>Streptococcus mutans</i> is a commensal member of the plaque microbiome. It is especially prevalent when dietary sugars are available for <i>S. mutans</i> fermentation, generating acid byproducts that lower plaque pH and foster tooth decay. <i>S. mutans</i> can survive in the transient conditions of the mouth, in part because it can regulate the uptake of manganese and iron during periods of feast when metal ions are available, and famine when they are limited. <i>S. mutans</i> depends on a 25kDa metalloregulatory protein, called SloR, to modulate the uptake of these cations across the bacterial cell surface. When bound to manganese, SloR binds to palindromic recognition elements in the promoter of the sloABC genes that encode the major manganese transporter in <i>S. mutans</i>. Reports in the literature describe MntH, an ancillary manganese transporter in <i>S. mutans</i>, that is also subject to SloR control. In the present study, we performed expression profiling experiments that reveal coordinate regulation of the sloABC and mntH genes at the level of transcription. In addition, we describe a role for the mntH gene product that is redundant with that of the sloABC-encoded metal ion uptake machinery. The results of DNA-binding studies support direct SloR binding to the mntH promoter region which, like that at the sloABC promoter, harbors three palindromic recognition elements to which SloR binds cooperatively to repress downstream transcription. These findings expand our understanding of the SloR metalloregulome and elucidate SloR-DNA binding that is essential for <i>S. mutans</i> metal ion homeostasis and fitness in the oral cavity.</p><p><strong>Importance: </strong>Dental caries disproportionately impacts low-income socioeconomic groups in the United States and abroad. Research that is focused on <i>S. mutans</i>, the primary causative agent of dental caries in humans, is significant to mitigation efforts aimed at alleviating or preventing dental caries. The SloR protein is a major regulator of the <i>S. mutans</i> metal ion uptake machinery encoded by the sloABC- and mntH genes. This SloR-mediated gene control is essential for maintaining intracellular metal ion homeostasis, and hence <i>S. mutans</i> fitness in the plaque microbiome. An improved understanding of the sloABC and mntH metal ion transporters and their regulation by SloR can guide rational drug design that, by targeting the SloR-DNA-binding interface, can alleviate or prevent <i>S. mutans</i>-induced disease.</p>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":" ","pages":"e0047024"},"PeriodicalIF":2.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The periplasmic and cytoplasmic faces of septal protein SepJ from filamentous cyanobacteria.","authors":"Enrique Flores","doi":"10.1128/jb.00488-24","DOIUrl":"https://doi.org/10.1128/jb.00488-24","url":null,"abstract":"<p><p>Filamentous, N₂-fixing, heterocyst-forming cyanobacteria grow as chains of cells in which intercellular transfer of regulators and metabolites takes place, allowing them to behave as multicellular organisms. Intercellular transfer occurs by diffusion through septal junctions. In the model heterocyst-forming cyanobacterium <i>Anabaena</i> sp. strain PCC 7120, some identified septal proteins, including FraC and FraD, are directly involved in the formation of junctions that have been visualized by cryo-electron tomography, whereas the role of the key septal protein SepJ remains elusive. SepJ can form tetramers and contains coiled-coil, linker, and integral membrane (permease) domains. Using AlphaFold 3, a SepJ tetramer is predicted to have a quaternary structure in which the coiled-coil domain traverses the cytoplasmic membrane through a cavity formed between the four permease domains. Part of the coiled-coil domain is thus located in the septal periplasm, where it can interact with peptidoglycan. This possible SepJ structure can be widespread in filamentous cyanobacteria and explains known properties of SepJ. Structures of SepJ with other septal proteins including SjcF1, SepI, and SepT could also be predicted consistent with their previously described interactions. A possible interaction of the SepJ coiled-coil domain with the catalytic domain of cell wall amidase AmiC1, which would be relevant to prevent filament fragmentation in <i>Anabaena</i>, is also discussed. The renewed view of SepJ presented here offers a molecular basis for understanding the key role of this protein in filament formation and intercellular communication.</p>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":" ","pages":"e0048824"},"PeriodicalIF":2.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Burkholderia cenocepacia</i>-mediated inhibition of <i>Staphylococcus aureus</i> growth and biofilm formation.","authors":"Tiffany J Brandt, Hayden Skaggs, Thomas Hundley, Deborah R Yoder-Himes","doi":"10.1128/jb.00116-23","DOIUrl":"https://doi.org/10.1128/jb.00116-23","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> asymptomatically colonizes the nasal cavity and pharynx of up to 60% of the human population and, as an opportunistic pathogen, can breach its normal habitat, resulting in life-threatening infections. <i>S. aureus</i> infections are of additional concern for populations with impaired immune function such as those with cystic fibrosis (CF) or chronic granulomatous disease. Multi-drug resistance is increasingly common in <i>S. aureus</i> infections, creating an urgent need for new antimicrobials or compounds that improve efficacy of currently available antibiotics. <i>S. aureus</i> biofilms, such as those found in the lungs of people with CF and in soft tissue infections, are notoriously recalcitrant to antimicrobial treatment due to the characteristic metabolic differences associated with a sessile mode of growth. In this work, we show that another CF pathogen, <i>Burkholderia cenocepacia</i>, produces one or more secreted compounds that can prevent <i>S. aureus</i> biofilm formation and inhibit existing <i>S. aureus</i> biofilms. The <i>B. cenocepacia</i>-mediated antagonistic activity is restricted to <i>S. aureus</i> species and perhaps some other staphylococci; however, this inhibition does not necessarily extend to other Gram-positive species. This inhibitory activity is due to death of <i>S. aureus</i> through a contact-independent mechanism, potentially mediated through the siderophore pyochelin and perhaps additional compounds. This works paves the way to better understanding of interactions between these two bacterial pathogens.IMPORTANCE<i>Staphylococcus aureus</i> is a major nosocomial pathogen responsible for infecting thousands of people each year. Some strains are becoming increasingly resistant to antimicrobials, and consequently new treatments must be sought. This paper describes the characterization of one or more compounds capable of inhibiting <i>S. aureus</i> biofilm formation and may potentially lead to development of a new therapeutic.</p>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":" ","pages":"e0011623"},"PeriodicalIF":2.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration and analytical techniques for membrane curvature-sensing proteins in bacteria.","authors":"Takumi Komikawa, Mina Okochi, Masayoshi Tanaka","doi":"10.1128/jb.00482-24","DOIUrl":"https://doi.org/10.1128/jb.00482-24","url":null,"abstract":"<p><p>The mechanism by which cells regulate protein localization is an important topic in the field of bacterial biology. In certain instances, the morphology of the biological membrane has been demonstrated to function as a spatial cue for the subcellular localization of proteins. These proteins are capable of sensing membrane curvature and are involved in a number of physiological functions such as cytokinesis and the formation of membrane-bound organelles. This review presents recent advances in the <i>in vitro</i> evaluation of curvature-sensing properties using artificially controlled membranes and purified proteins, as well as microscopic live cell assays. However, these evaluation methodologies often require sophisticated experiments, and the number of identified curvature sensors remains limited. Thus, we present a comprehensive exploration of recently reported curvature-sensing proteins. Subsequently, we summarize the known curvature-sensing proteins in bacteria, in conjunction with the analytical methodologies employed in this field. Finally, future prospects and further requirements in the study of curvature-sensing proteins are discussed.</p>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":" ","pages":"e0048224"},"PeriodicalIF":2.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}