Journal of Bacteriology最新文献

{"title":"Biological puzzles solved by using <i>Streptococcus pneumoniae</i>: a historical review of the pneumococcal studies that have impacted medicine and shaped molecular bacteriology.","authors":"N Luisa Hiller, Carlos J Orihuela","doi":"10.1128/jb.00059-24","DOIUrl":"10.1128/jb.00059-24","url":null,"abstract":"<p><p>The major human pathogen <i>Streptococcus pneumoniae</i> has been the subject of intensive clinical and basic scientific study for over 140 years. In multiple instances, these efforts have resulted in major breakthroughs in our understanding of basic biological principles as well as fundamental tenets of bacterial pathogenesis, immunology, vaccinology, and genetics. Discoveries made with <i>S. pneumoniae</i> have led to multiple major public health victories that have saved the lives of millions. Studies on <i>S. pneumoniae</i> continue today, where this bacterium is being used to dissect the impact of the host on disease processes, as a powerful cell biology model, and to better understand the consequence of human actions on commensal bacteria at the population level. Herein we review the major findings, i.e., puzzle pieces, made with <i>S. pneumoniae</i> and how, over the years, they have come together to shape our understanding of this bacterium's biology and the practice of medicine and modern molecular biology.</p>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11332154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repression of <i>Acinetobacter baumannii</i> DNA damage response requires DdrR-assisted binding of UmuDAb dimers to atypical SOS box.","authors":"Belinda Candra, Deborah Cook, Janelle Hare","doi":"10.1128/jb.00432-23","DOIUrl":"10.1128/jb.00432-23","url":null,"abstract":"<p><p>The DNA damage response of the multi-drug-resistant nosocomial pathogen <i>Acinetobacter baumannii</i> possesses multiple features that distinguish it from the commonly used LexA repression system. These include the absence of LexA in this genus, the evolution of a UmuD polymerase manager into the UmuDAb repressor of error-prone polymerases, the use of a corepressor unique to <i>Acinetobacter</i> (DdrR), and an unusually large UmuDAb binding site. We defined cis- and trans-acting factors required for UmuDAb DNA binding and gene repression, and tested whether DdrR directly enhances its DNA binding. We used DNA binding assays to characterize UmuDAb's binding to its proposed operator present upstream of the six co-repressed <i>umuDC</i> or <i>umuC</i> genes. UmuDAb bound tightly and cooperatively to this site with ~10-fold less affinity than LexA. DdrR enhanced the binding of both native and dimerization-deficient UmuDAb forms, but only in greater than equimolar ratios relative to UmuDAb. UmuDAb mutants unable to dimerize or effect gene repression showed impaired DNA binding, and a strain expressing the G124D dimerization mutant could not repress transcription of the UmuDAb-DdrR regulon. Competition electrophoretic mobility shift assays conducted with mutated operator probes showed that, unlike typical SOS boxes, the UmuDAb operator possessed a five-base pair central core whose sequence was more crucial for binding than the flanking palindrome. The presence of only one of the two flanking arms of the palindrome was necessary for UmuDAb binding. Overall, the data supported a model of an operator with two UmuDAb binding sites. The distinct characteristics of UmuDAb and its regulated promoters differ from the typical LexA repression model, demonstrating a novel method of repression.IMPORTANCE<i>Acinetobacter baumannii</i> is a gram-negative bacterium responsible for hospital-acquired infections. Its unique DNA damage response can activate multiple error-prone polymerase genes, allowing it to gain mutations that can increase its virulence and antibiotic resistance. The emergence of infectious strains carrying multiple antibiotic resistance genes, including carbapenem resistance, lends urgency to discovering and developing ways to combat infections resistant to treatment with known antibiotics. Deciphering how the regulators UmuDAb and DdrR repress the error-prone polymerases could lead to developing complementary treatments to halt this mechanism of generating resistance.</p>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11332147/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140898230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asking a question.","authors":"Roberto Kolter","doi":"10.1128/jb.00050-24","DOIUrl":"10.1128/jb.00050-24","url":null,"abstract":"<p><p>While scientific research should be carried out objectively, the choices of questions asked and approaches taken are deeply personal and subjective. I urge individuals to pursue questions they love and to periodically scrutinize the reasons (the philosophies) that drive that love. As a case study, I scrutinize the \"whys\" behind some of the scientific questions I pursued during my career.</p>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11332143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141179670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In memoriam: Pierre Cornelis (1949-2023).","authors":"Jean-Paul Pirnay, Françoise Van Bambeke, Christine Baysse, Miguel Cámara, Sylvie Chevalier, Jean François Collet, Aurélie Crabbé, Jozef Dingemans, Alain Dufour, Linda Eeckhaudt, Alain Filloux, Olivier Lesouhaitier, Qing Wei, Daniel De Vos","doi":"10.1128/jb.00179-24","DOIUrl":"https://doi.org/10.1128/jb.00179-24","url":null,"abstract":"","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lamotrigine-mediated rescue of RsgA-deficient Escherichia coli reveals another role of IF2 in ribosome biogenesis","authors":"Sudhir Singh, Jitendra Singh, Umesh Varshney","doi":"10.1128/jb.00119-24","DOIUrl":"https://doi.org/10.1128/jb.00119-24","url":null,"abstract":"RsgA is a late-stage ribosome biogenesis factor. Earlier, infB (IF2) was isolated as a multicopy suppressor of the Escherichia coli ΔrsgA strain. How IF2 rescued the strain growth remained unclear. This study reveals that\u0000(i) the multicopy infB-mediated ...","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141259663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Ssc, an <i>N</i>-acetylgalactosamine-containing <i>Staphylococcus aureus</i> surface polysaccharide.","authors":"Mei G Lei, Matthew A Jorgenson, Emily J Robbs, Ian M Black, Stephanie Archer-Hartmann, Sergei Shalygin, Parastoo Azadi, Chia Y Lee","doi":"10.1128/jb.00048-24","DOIUrl":"10.1128/jb.00048-24","url":null,"abstract":"<p><p>Whole genome sequencing has revealed that the genome of <i>Staphylococcus aureus</i> possesses an uncharacterized 5-gene operon (SAOUHSC_00088-00092 in strain 8325 genome) that encodes factors with functions related to polysaccharide biosynthesis and export, indicating the existence of a new extracellular polysaccharide species. We designate this locus as <i>ssc</i> for staphylococcal surface carbohydrate. We found that the <i>ssc</i> genes were weakly expressed and highly repressed by the global regulator MgrA. To characterize Ssc, Ssc was heterologously expressed in <i>Escherichia coli</i> and extracted by heat treatment. Ssc was also conjugated to AcrA from <i>Campylobacter jejuni</i> in <i>E. coli</i> using protein glycan coupling technology (PGCT). Analysis of the heat-extracted Ssc and the purified Ssc-AcrA glycoconjugate by tandem mass spectrometry revealed that Ssc is likely a polymer consisting of <i>N</i>-acetylgalactosamine. We further demonstrated that the expression of the <i>ssc</i> genes in <i>S. aureus</i> affected phage adsorption and susceptibility, suggesting that Ssc is surface-exposed.</p><p><strong>Importance: </strong>Surface polysaccharides play crucial roles in the biology and virulence of bacterial pathogens. <i>Staphylococcus aureus</i> produces four major types of polysaccharides that have been well-characterized. In this study, we identified a new surface polysaccharide containing N-acetylgalactosamine (GalNAc). This marks the first report of GalNAc-containing polysaccharide in <i>S. aureus</i>. Our discovery lays the groundwork for further investigations into the chemical structure, surface location, and role in pathogenesis of this new polysaccharide.</p>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11112989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140850748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In a state of flux: new insight into the transport processes that maintain bacterial metal homeostasis.","authors":"Natalia Kwiatos, Kevin J Waldron","doi":"10.1128/jb.00146-24","DOIUrl":"10.1128/jb.00146-24","url":null,"abstract":"<p><p>A new study by Nies et al. (J Bacteriol 206:e00080-24, 2024, https://doi.org/10.1128/jb.00080-24) provides a rich, quantitative data set of zinc accumulation by cells of <i>Cupriavidus metallidurans</i>, including of mutant bacterial strains lacking import or efflux genes, and comparison of zinc accumulation by cells previously starved of metal with those of zinc-replete cells. The data surprisingly demonstrate the concomitant activity of both active metal import and metal efflux systems. They present a flow equilibrium model to describe zinc homeostasis in bacteria.</p>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11112988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140854895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional consequences of modification of the photosystem I/photosystem II ratio in the cyanobacterium Synechocystis sp. PCC 6803","authors":"Vicki MooreWim Vermaas1School of Life Sciences and Center for Bioenergy and Photosynthesis, Arizona State University, Tempe, Arizona, USA, Conrad W. Mullineaux","doi":"10.1128/jb.00454-23","DOIUrl":"https://doi.org/10.1128/jb.00454-23","url":null,"abstract":"Journal of Bacteriology, Ahead of Print. <br/>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140827448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lighting the way: how the Vibrio fischeri model microbe reveals the complexity of Earth’s “simplest” life forms","authors":"Alecia N. Septer, Karen L. Visick","doi":"10.1128/jb.00035-24","DOIUrl":"https://doi.org/10.1128/jb.00035-24","url":null,"abstract":"Life is thought to have evolved in the ocean before expanding to terrestrial habitats.\u0000It is no wonder then that marine bacteria share genes, physiology, functional pathways,\u0000and regulatory mechanisms with their terrestrial cousins. These pathways and processes\u0000represent deeply rooted, conserved strategies that have evolved over billions of years\u0000and adapted to new habitats as the Earth and its increasingly diverse host species\u0000changed over time.","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140827451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted deletion of Pf prophages from diverse Pseudomonas aeruginosa isolates has differential impacts on quorum sensing and virulence traits","authors":"Amelia K. SchmidtCaleb M. SchwartzkopfJulie D. PourtoisElizabeth B. BurgenerDominick R. FaithAlex JoyceTyrza LammaGeetha KumarPaul L. BollykyPatrick R. Secor1Division of Biological Sciences, University of Montana, Missoula, Montana, USA2Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California, USA3Division of Pediatric Pulmonology and Sleep Medicine, Children’s Hospital of Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, California, USA4Center for Excellence in Pulmonary Biology, Department of Pediatrics, Stanford University, Stanford, California, USA5School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Kerala, India, Joseph Bondy-Denomy","doi":"10.1128/jb.00402-23","DOIUrl":"https://doi.org/10.1128/jb.00402-23","url":null,"abstract":"Journal of Bacteriology, Ahead of Print. <br/>","PeriodicalId":15107,"journal":{"name":"Journal of Bacteriology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140827626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}