Journal of Avian Medicine and Surgery最新文献

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Journal of Avian Medicine and Surgery Masthead 禽医学与外科杂志报头
4区 农林科学
Journal of Avian Medicine and Surgery Pub Date : 2023-11-10 DOI: 10.1647/1082-6742-37.3.fmi
{"title":"Journal of Avian Medicine and Surgery Masthead","authors":"","doi":"10.1647/1082-6742-37.3.fmi","DOIUrl":"https://doi.org/10.1647/1082-6742-37.3.fmi","url":null,"abstract":"","PeriodicalId":15102,"journal":{"name":"Journal of Avian Medicine and Surgery","volume":"110 36","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135137515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of Atorvastatin and Rosuvastatin on Blood Lipids in Quaker Parrots (Myiopsitta monachus). 阿托伐他汀和瑞舒伐他汀对桂格鹦鹉血脂的影响。
IF 0.3 4区 农林科学
Journal of Avian Medicine and Surgery Pub Date : 2023-11-01 DOI: 10.1647/22-00014
Hugues Beaufrère, Trinita Barboza, Alysha Burnett, Ken D Stark, R Darren Wood
{"title":"Effects of Atorvastatin and Rosuvastatin on Blood Lipids in Quaker Parrots (<i>Myiopsitta monachus</i>).","authors":"Hugues Beaufrère, Trinita Barboza, Alysha Burnett, Ken D Stark, R Darren Wood","doi":"10.1647/22-00014","DOIUrl":"10.1647/22-00014","url":null,"abstract":"<p><p>Statin drugs are the most effective class of hypolipidemic and antiatherosclerotic drugs, with atorvastatin and rosuvastatin being the most effective. While the use of statins would be a tremendous asset in the treatment of dyslipidemia and lipid-accumulation disorders in birds, there are only limited data available regarding their use and effectiveness in psittacine species. Two consecutive randomized crossover trials on Quaker parrots (<i>Myiopsitta monachus</i>) were performed to study the effect of atorvastatin and rosuvastatin. Ten birds were used in an initial balanced crossover experiment with 5 oral treatments (control; atorvastatin 10 mg/kg q12h and q24h; rosuvastatin 10 mg/kg q12h and q24h) for 2 weeks each. Plasma lipidomics and lipoprotein profiling were performed after each treatment. Twelve birds were used in a second experiment consisting of 2 parallel crossover studies, each with 6 birds either fed their regular diet or a 0.3% cholesterol diet. In the 2 parallel crossover studies, the treatment group was administered atorvastatin 20 mg/kg orally q12h and the control group a placebo suspension orally q12h. Plasma lipidomics, lipoprotein profiles, and 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase activity were subsequently measured. Results were analyzed with serial linear mixed models and trends were assessed graphically. No statistically significant effect of any statin treatment was detected on plasma lipids, lipoproteins, creatinine kinase, or HMG-CoA reductase activity. In the first trial, all the rosuvastatin treatments led to some nonsignificant decreases in several triacylglycerol species, while in the second trial this was only observed in the birds on atorvastatin 20 mg/kg q12h being fed their regular diet. Quaker parrots may require much higher doses of statin drugs to show significant and clinically useful lipid-lowering effects.</p>","PeriodicalId":15102,"journal":{"name":"Journal of Avian Medicine and Surgery","volume":"37 3","pages":"199-208"},"PeriodicalIF":0.3,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92154415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Survey of the Clinical, Cytological, and Histopathological Features Associated with Neoplasms in Captive Avian Species in Melbourne, Australia. 澳大利亚墨尔本圈养鸟类中与肿瘤相关的临床、细胞学和组织病理学特征调查。
IF 0.3 4区 农林科学
Journal of Avian Medicine and Surgery Pub Date : 2023-11-01 DOI: 10.1647/20-00056
Michelle Sutherland, Kathy Luk, Natalie Courtman, Richard Ploeg
{"title":"Survey of the Clinical, Cytological, and Histopathological Features Associated with Neoplasms in Captive Avian Species in Melbourne, Australia.","authors":"Michelle Sutherland, Kathy Luk, Natalie Courtman, Richard Ploeg","doi":"10.1647/20-00056","DOIUrl":"10.1647/20-00056","url":null,"abstract":"<p><p>Over a 3.5-year period, tissue samples from 141 companion and aviary birds with clinically suspected, naturally occurring solid neoplasms were collected via surgical biopsy (n = 53) or at necropsy examination (n = 88) from a population of birds presenting to an avian veterinary clinic in Melbourne, Australia. Neoplastic processes were identified in 73.7% (n = 104) of samples, with 83.7% (n = 87) being interpreted as malignant neoplasia and 16.3% (n = 17) being interpreted as benign neoplasia. The overall estimated prevalence of neoplasia in the study population (n = 5125) was 2.45% (95% confidence interval, 2-2.97%). The gastrointestinal and reproductive tracts were the most commonly affected systems. The most common presenting signs were nonspecific and included lethargy, coelomic distension, and inappetence. In 59 cases, fine-needle aspirates (FNAs) and impression smears were also obtained and evaluated cytologically. The accuracy of cytology for detecting neoplasia in birds and for determining whether a neoplastic process was benign or malignant was assessed by using histopathology as the \"gold standard.\" There was complete agreement between the cytological and histopathological diagnoses in 72.8% (43/59) of cases. Cytology correctly identified 87.5% of these cases (35/40) as malignant neoplasms and 55.6% (5/9) as benign processes. There was no significant difference between the use of cytology and histopathology for the detection of malignant neoplasia in birds (<i>P</i> = 0.185). The accuracies of FNAs and impression smears for examining avian tumors were also compared. Overall, the best cell preservation was obtained by performing impression smears from tissues, with 62.2% (n = 28) returning high cellularity for cytological examination, compared to 53.8% (n = 14) when samples were obtained by FNA. This study provides an overview of the types and prevalence of neoplasms in a captive bird population from Australia, correlates physical examination findings with tumor types, and provides evidence that cytology is a reliable preliminary diagnostic tool for detecting neoplasia in birds.</p>","PeriodicalId":15102,"journal":{"name":"Journal of Avian Medicine and Surgery","volume":"37 3","pages":"243-265"},"PeriodicalIF":0.3,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92154420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction of a Hyperflexed Hallux in a Saker Falcon (Falco cherrug) by Hemisectioning the Deep Digital Flexor Tendon. 半切指深屈肌腱矫治Saker Falcon (Falco cherrug)过度屈曲拇趾。
IF 0.3 4区 农林科学
Journal of Avian Medicine and Surgery Pub Date : 2023-11-01 DOI: 10.1647/22-00062
Raj Raghav, Vishnunarayanan Narayanannair, Jaime Samour
{"title":"Correction of a Hyperflexed Hallux in a Saker Falcon (<i>Falco cherrug</i>) by Hemisectioning the Deep Digital Flexor Tendon.","authors":"Raj Raghav, Vishnunarayanan Narayanannair, Jaime Samour","doi":"10.1647/22-00062","DOIUrl":"10.1647/22-00062","url":null,"abstract":"<p><p>A 3-year-old male saker falcon (<i>Falco cherrug</i>) was presented with a history of hyperflexion of the first digit of the left pelvic limb with what appeared to be a hypercontracted distal end of the deep digital flexor tendon (<i>m. flexor hallucis longus</i>). Conservative treatment, bandaging, and splinting techniques failed to resolve the hypercontraction of the deep digital flexor tendon and improve digit function. Intraoperatively, the deep digital flexor tendon was found to have formed adhesions with the tendon sheath as well as the bone and soft tissue structures underneath it, impeding its ability to move freely within the tendon sheath. The adhesions were surgically resolved, and the tendon was lengthened by hemisectioning the deep digital flexor tendon at its distal end. Postsurgically, the digit was maintained in an extended position by bandaging. Mild physiotherapy was provided at 5-day intervals to retain function and prevent adhesions. Digit extensibility and function were restored to nearly normal levels after 4 weeks.</p>","PeriodicalId":15102,"journal":{"name":"Journal of Avian Medicine and Surgery","volume":"37 3","pages":"275-281"},"PeriodicalIF":0.3,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92154404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diagnosis and Long-term Treatment of Suspected Congenital Hypothyroidism in a Pigeon (Columba livia domestica). 家鸽疑似先天性甲状腺功能减退症的诊断与长期治疗。
IF 0.3 4区 农林科学
Journal of Avian Medicine and Surgery Pub Date : 2023-11-01 DOI: 10.1647/AVMS-D-22-00064
Nicola Di Girolamo, Kelsea Studer, João Brandão, Ian Kanda, Robert A Wagner, Federico Fracassi, Kellie Fecteau
{"title":"Diagnosis and Long-term Treatment of Suspected Congenital Hypothyroidism in a Pigeon (<i>Columba livia domestica</i>).","authors":"Nicola Di Girolamo, Kelsea Studer, João Brandão, Ian Kanda, Robert A Wagner, Federico Fracassi, Kellie Fecteau","doi":"10.1647/AVMS-D-22-00064","DOIUrl":"10.1647/AVMS-D-22-00064","url":null,"abstract":"<p><p>An approximately 6-month-old domestic pigeon (<i>Columba livia domestica</i>) was presented for lethargy and an inability to perform its first molt. The pigeon was obese, had anatomical characteristics of a chick, including cere and plumage, and had a ventral coelomic soft tissue mass. Initial blood work was unremarkable. A computed tomographic scan confirmed excessive fat deposition in the coelom and a mass adherent to the liver. A fine-needle aspirate of the mass indicated fat accumulation. A thyroid-stimulating hormone (TSH) test was planned for this pigeon and 3 presumed euthyroid pigeons. Each pigeon was administered 80 µg (∼230 µg/kg) of recombinant human TSH. Blood was drawn at time 0 and 3 and 6 hours after administration of recombinant human TSH. Plasma total thyroxine (TT<sub>4</sub>) was measured in duplicate with an in-house analyzer and a reference laboratory. After recombinant human TSH administration, healthy pigeons showed a 4- to 21-fold increase in TT<sub>4</sub>, whereas the hypothyroid pigeon had all values <0.12 µg/dL. The pigeon was prescribed 20 µg of compounded levothyroxine twice daily. In the following months, the pigeon molted and developed adult features. The ventral coelomic soft tissue mass disappeared and repeated computed tomography scans showed a decreased amount of body fat and a reduction in the size of the coelomic mass. Levothyroxine was further adjusted multiple times according to additional TT<sub>4</sub> testing to a dose of 2.5 µg once daily. The pigeon has been under treatment with levothyroxine for more than 2 years. Here we present the first reported case of confirmed hypothyroidism in a pigeon. Diagnosis with a TSH stimulation test was unequivocal, even when only considering the results of the in-house analyzer. Levothyroxine treatment resolved clinical signs and could be titrated to an appropriate dose.</p>","PeriodicalId":15102,"journal":{"name":"Journal of Avian Medicine and Surgery","volume":"37 3","pages":"282-287"},"PeriodicalIF":0.3,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92154414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Thermal Antinociceptive Effects of Intramuscular Hydromorphone Hydrochloride in Great Horned Owls (Bubo virginianus). 肌肉注射盐酸氢吗啡酮对大角猫头鹰热抗伤感受作用的评价。
IF 0.3 4区 农林科学
Journal of Avian Medicine and Surgery Pub Date : 2023-11-01 DOI: 10.1647/JAMS-D-22-00063
Marissa Rae Monopoli, David Sanchez-Migallon Guzman, Joanne Paul-Murphy, Hugues Beaufrère, Michelle G Hawkins
{"title":"Evaluation of Thermal Antinociceptive Effects of Intramuscular Hydromorphone Hydrochloride in Great Horned Owls (<i>Bubo virginianus</i>).","authors":"Marissa Rae Monopoli, David Sanchez-Migallon Guzman, Joanne Paul-Murphy, Hugues Beaufrère, Michelle G Hawkins","doi":"10.1647/JAMS-D-22-00063","DOIUrl":"10.1647/JAMS-D-22-00063","url":null,"abstract":"<p><p>Across the Americas, great horned owls (<i>Bubo virginianus</i>) are often presented to veterinarians for conditions requiring pain management. Although recent studies have evaluated opioid drugs in raptor species, information in Strigiformes is lacking. The objective of this study was to evaluate the analgesic effect and duration of action of hydromorphone hydrochloride, a full µ-opioid receptor agonist, in great horned owls. In a randomized, blinded, balanced crossover study, 6 adult birds (5 females and 1 male) received hydromorphone (0.3 and 0.6 mg/kg) or saline (0.9% NaCl) solution (0.03 mL/kg; control) in the left pectoral muscle, with a 7-day washout interval between treatments. Each bird was assigned an agitation-sedation score, and the thermal foot withdrawal threshold (TFWT) was measured at predetermined times before (<i>t</i> = 0 hours) and after treatment administration (<i>t</i> = 0.5, 1.5, 3, and 6 hours). Measurements of the TFWT were obtained with a test box equipped with a thermal perch, which delivered a gradually increasing temperature 40-62°C (104-143.6°F) to the right plantar surface of the owl's foot. Compared with controls, hydromorphone at 0.3 mg/kg dose resulted in significantly higher mean TFWT at 0.5 hours (<i>P</i> < 0.001), 1.5 hours (<i>P</i> = 0.003), and 3 hours (<i>P</i> = 0.005), whereas the 0.6 mg/kg dose resulted in significantly higher mean TFWT from 0.5 hours (<i>P</i> = 0.035) to 1.5 hours (<i>P</i> = 0.001). Both hydromorphone doses were associated with a significant change in the agitation-sedation score (<i>P</i> = 0.001), consistent with mild to moderate sedation. Two owls were observed tremoring after administration of the 0.6 mg/kg dose, which was not noted after the 0.5-hour timepoint; no other adverse effects were identified. This study offers scientific evidence to support the use of a µ-opioid agonist in great horned owls for pain management. Pharmacokinetics and other pharmacodynamic studies of other pain models evaluating hydromorphone and other opioid drugs in this species are still needed.</p>","PeriodicalId":15102,"journal":{"name":"Journal of Avian Medicine and Surgery","volume":"37 3","pages":"209-216"},"PeriodicalIF":0.3,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92154416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Four Different Surgical Approaches to Vasectomies in Male Texas Bobwhite Quail (Colinus virginianus texanus) and Northern Bobwhite Quail (Colinus virginianus). 四种不同手术方法在雄性德州山鹑和北方山鹑输精管切除术中的应用。
IF 0.3 4区 农林科学
Journal of Avian Medicine and Surgery Pub Date : 2023-11-01 DOI: 10.1647/2021-0132
Stephanie K Lamb, Glenn H Olsen, Anthony Pilny
{"title":"Four Different Surgical Approaches to Vasectomies in Male Texas Bobwhite Quail (<i>Colinus virginianus texanus</i>) and Northern Bobwhite Quail (<i>Colinus virginianus</i>).","authors":"Stephanie K Lamb, Glenn H Olsen, Anthony Pilny","doi":"10.1647/2021-0132","DOIUrl":"10.1647/2021-0132","url":null,"abstract":"<p><p>Vasectomies render a male sterile and have been used for various management purposes, including conservation efforts. This report evaluated 4 different surgical approaches (external approach, internal approach with dissection, internal approach with cautery, and internal approach caudally) to perform 177 vasectomies in Texas bobwhite (<i>Colinus virginianus texanus</i>; n = 171) and northern bobwhite quail (<i>Colinus virginianus</i>; n = 6) in a field setting. Birds were not randomized into groups for the different approaches. Survival was recorded in 83% (147/177) of the birds. The most common cause of death was hemorrhage from the common iliac vein due to damage during the surgical procedure. Other causes for death included transection of the ureter, parasitism, euthanasia, and undetermined causes. The approach that had the highest survival rate (89.8%, 132/147) was the internal approach with cautery, and based on these results the authors recommend this approach for vasectomies in Texas and northern bobwhite quail.</p>","PeriodicalId":15102,"journal":{"name":"Journal of Avian Medicine and Surgery","volume":"37 3","pages":"226-234"},"PeriodicalIF":0.3,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92154417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Retrospective Study of Intertarsal Joint Inflammation in Avian Species From a Zoological Institution. 某动物研究所鸟类跗关节间炎症的回顾性研究。
IF 0.3 4区 农林科学
Journal of Avian Medicine and Surgery Pub Date : 2023-11-01 DOI: 10.1647/D-22-00054
Antoine Leclerc, Nicolas Goddard, Océane Graillot, Alice Brunet, Baptiste Mulot
{"title":"Retrospective Study of Intertarsal Joint Inflammation in Avian Species From a Zoological Institution.","authors":"Antoine Leclerc, Nicolas Goddard, Océane Graillot, Alice Brunet, Baptiste Mulot","doi":"10.1647/D-22-00054","DOIUrl":"10.1647/D-22-00054","url":null,"abstract":"<p><p>A retrospective cross-sectional study was performed to measure the prevalence of avian intertarsal inflammation over a 5-year period, identify risk factors, and discuss treatment options. The authors hypothesized that: 1) long-legged birds would be more affected, 2) participation in a bird show would be a significant risk factor, and 3) young animals would be more frequently affected. Thirty-five clinical cases from 9 avian orders were included in the study. Statistical analysis indicated that the orders Ciconiiformes (9/150; 6%, 95% confidence interval [CI]: 3.2-11), Gruiformes (4/132; 3%, 95% CI: 1.2-7.5), and Pelecaniformes (7/152; 2.8%, 95% CI: 1.4-5.6) were significantly more affected than other orders (<i>P</i> < 0.01). Similarly, long-legged birds (21/35) had 9.8 times greater chance (<i>P</i> < 0.001, 95% CI: 4.7-21) of developing the condition compared with other birds. Participation in a free-flight show (22/35) was a significant risk factor (<i>P</i> < 0.001; odds ratio: 7.0, 95% CI: 3.3-15). Mean age at onset of clinical signs was 5.7 years, and being < 2 years-of-age during the study period was not a significant predictor of disease (<i>P</i> = 0.054). The tibial cartilage, a fragile fibrocartilaginous structure, was frequently affected (34%, 12/35). Treatment protocols included anti-inflammatory drugs, analgesic drugs, or both (94%, 33/35), low-level laser therapy (54%, 19/35), joint immobilization (34%, 12/35), intra-articular corticoid injections (20%, 7/35), surgical stabilization (17%, 6/35), physiotherapy (9%, 3/35), intra-articular hyaluronic acid (6%, 2/35) or platelet-rich plasma (3%, 1/35) injections, and chiropractic care (3%, 1/35). Overall recovery rate was 49% (17/35), and the condition was associated with a poor prognosis in chronic cases.</p>","PeriodicalId":15102,"journal":{"name":"Journal of Avian Medicine and Surgery","volume":"37 3","pages":"235-242"},"PeriodicalIF":0.3,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92154419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of Freezing Temperature, Freezing Duration, and Repeated Freeze/Thaw Cycles on Electrophoretic Profiles in the White Stork (Ciconia ciconia). 冻结温度、冻结时间和反复冻融循环对白鹳电泳谱的影响。
IF 0.3 4区 农林科学
Journal of Avian Medicine and Surgery Pub Date : 2023-11-01 DOI: 10.1647/22-00051
Milan Thorel, Yannick Roman, Antoine Leclerc
{"title":"Influence of Freezing Temperature, Freezing Duration, and Repeated Freeze/Thaw Cycles on Electrophoretic Profiles in the White Stork (<i>Ciconia ciconia</i>).","authors":"Milan Thorel, Yannick Roman, Antoine Leclerc","doi":"10.1647/22-00051","DOIUrl":"10.1647/22-00051","url":null,"abstract":"<p><p>Plasma electrophoresis is an ancillary diagnostic tool in avian medicine, with agarose gel electrophoresis (AGE) and capillary zone electrophoresis (CZE) being the most common techniques. Frozen samples can be used for quantitative studies or comparative diagnostic purposes, but stability of avian plasma proteins under freezing is poorly described. To evaluate the influence of plasma freezing on electrophoretograms in white storks (<i>Ciconia ciconia</i>), heparin blood was sampled from 30 individuals during annual health examinations. Plasma samples were obtained after centrifugation of fresh samples and divided into aliquots. Both AGE and CZE were performed on fresh aliquots. The remaining aliquots were frozen at -20°C (-4°F) or -180°C (-292°F) and thawed following different protocols: 1 freeze/thaw cycle after 6 months at -20°C; 1, 2, 4, and 7 cycles over 12 months at -20°C; and 1 cycle after 18 months at -180°C. For both techniques, electrophoretic profiles obtained from these thawed aliquots were compared to fresh electrophoretograms. Quantitatively, significant differences (<i>P</i> < 0.05) in most fractions were seen from 6 months postfreezing at -20°C for both techniques. Fewer statistically significant differences were observed after 18 months under cryogenic preservation (-180°C). Qualitatively, AGE provided more repeatable and stable results than CZE over time on samples stored at -20°C, and electrophoretograms were stable after 18 months of cryogenic storage. An electromigration distortion associated with freezing was seen with CZE only. Plasma samples stored in a conventional freezer (-20°C) should not be compared to fresh plasma. For quantitative studies, cryogenic storage should be privileged.</p>","PeriodicalId":15102,"journal":{"name":"Journal of Avian Medicine and Surgery","volume":"37 3","pages":"266-274"},"PeriodicalIF":0.3,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92154418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Thank You, Dr. Bob Groskin! 谢谢你,鲍勃·格罗斯金医生!
IF 0.3 4区 农林科学
Journal of Avian Medicine and Surgery Pub Date : 2023-11-01 DOI: 10.1647/1082-6742-37.3.197
Thomas N Tully, Michelle G Hawkins, Mark A Mitchell
{"title":"Thank You, Dr. Bob Groskin!","authors":"Thomas N Tully, Michelle G Hawkins, Mark A Mitchell","doi":"10.1647/1082-6742-37.3.197","DOIUrl":"10.1647/1082-6742-37.3.197","url":null,"abstract":"","PeriodicalId":15102,"journal":{"name":"Journal of Avian Medicine and Surgery","volume":"37 3","pages":"197-198"},"PeriodicalIF":0.3,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92154421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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