Journal of Asia-pacific Entomology最新文献

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Tick repellent activities of supercritical fluid extract from Chamaecyparis obtusa against Haemaphysalis longicornis 从 Chamaecyparis obtusa 中提取的超临界流体提取物对长角蜱的驱蜱活性
IF 1.1 3区 农林科学
Journal of Asia-pacific Entomology Pub Date : 2024-07-28 DOI: 10.1016/j.aspen.2024.102300
Young Su Jang
{"title":"Tick repellent activities of supercritical fluid extract from Chamaecyparis obtusa against Haemaphysalis longicornis","authors":"Young Su Jang","doi":"10.1016/j.aspen.2024.102300","DOIUrl":"10.1016/j.aspen.2024.102300","url":null,"abstract":"<div><p>Supercritical fluid extraction (SFE) is a rapid, selective, and convenient method for sample preparation prior to the analysis of compounds in a volatile product of plant matrices. In this study, insecticidal activity and repellency were examined in a laboratory study to determine the active constituent of <em>Chamaeparis obtusa</em> and a supercritical fluid extract from <em>Chamaeparis obtusa</em> against hard tick <em>Haemaphysalis longicornis.</em></p><p>The repellency of <em>C. obtusa</em> oil was evaluated in comparison with two commercial repellents (Icaridin and DEET) against <em>H. longicornis</em>. Filter-paper diffusion assay revealed that <em>C. obtusa</em> oil (3.2 µg/cm<sup>2</sup>) had a 100 % repellency and icaridine (3.2 µg/cm<sup>2</sup>) had a 96.1 % repellency at 120 min. Repellencies of 31 compounds against <em>H. longicornis</em> were compared with the repellency of DEET at 3.2 µg/cm<sup>2</sup>. Responses varied according to the test compound and exposure time. Repellencies of α-terpinene, terpinene-4-ol, and α-terpineol were 100 % at 30 min post-treatment. They were 85.5 %, 56.4 %, and 68.9 %, respectively, at 120 min. <em>C. obtusa</em> oil, <em>α</em>-terpinene, terpinene-4-ol, and <em>α</em>-terpineol merit further study as potential fumigants or lead compounds for the control of <em>H. longicornis</em>.</p></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"27 3","pages":"Article 102300"},"PeriodicalIF":1.1,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1226861524001055/pdfft?md5=a11a07572f02285b795bf33c4b37f435&pid=1-s2.0-S1226861524001055-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141839200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bacterial communities of two populations of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) 两个种群的细菌群落
IF 1.1 3区 农林科学
Journal of Asia-pacific Entomology Pub Date : 2024-07-19 DOI: 10.1016/j.aspen.2024.102295
Shahnaz Zare Banadkuki , Shima Rahmani , Ali R. Bandani
{"title":"Bacterial communities of two populations of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)","authors":"Shahnaz Zare Banadkuki ,&nbsp;Shima Rahmani ,&nbsp;Ali R. Bandani","doi":"10.1016/j.aspen.2024.102295","DOIUrl":"10.1016/j.aspen.2024.102295","url":null,"abstract":"<div><p>The tomato leafminer, <em>Tuta absoluta</em> (Meyrick) (Lepidoptera: Gelechiidae), is a highly destructive insect pest in Solanaceae, particularly in tomato crops. In this study, bacteria symbionts of eggs, the whole body, and different tissues of the fourth instar larvae of two populations, Rasht and Karaj, were isolated and identified. Using the 16S rRNA procedure, all symbiont bacteria, whether cultured or non-cultured, were classified at the phylum level as Proteobacteria and Firmicutes. BLASTn analyses revealed an overlap of Wolbachia, Enterobacter, Pseudomonas, Staphylococcus, and Acinetobacter genera in both populations. However, <em>Bacillus subtilis</em>, and <em>Serratia marcescens</em> were found only in Rasht, while Paenibacillus sp. and P. pasadenensis were identified solely in the Karaj population. Phylogenetic analysis of all bacterial sequences, except <em>Wolbachia</em>, revealed a distant evolutionary relationship between the leafminer's symbionts and those of other lepidopterans. Thus, we could demonstrate the microbiota diversity of this insect pest across different locations. Furthermore, this research will provide insights for discovering new biorational control methods for T. absoluta based on the parasitic or mutualist symbionts.</p></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"27 3","pages":"Article 102295"},"PeriodicalIF":1.1,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141778510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Length-weight relationships of moths along the elevational gradient of three mountains across southern South Korea 南朝鲜南部三座山脉海拔梯度上蛾类的长重关系
IF 1.1 3区 农林科学
Journal of Asia-pacific Entomology Pub Date : 2024-07-18 DOI: 10.1016/j.aspen.2024.102294
Sei-Woong Choi
{"title":"Length-weight relationships of moths along the elevational gradient of three mountains across southern South Korea","authors":"Sei-Woong Choi","doi":"10.1016/j.aspen.2024.102294","DOIUrl":"10.1016/j.aspen.2024.102294","url":null,"abstract":"<div><p>Organism size significantly influences various aspects of physiology and ecology. In insects, body size can be assessed using parameters such as wing length, thorax width, abdomen length, and body weight. Among Lepidoptera, wing length is a commonly used metric for measuring body size, although obtaining data on body weight can be challenging. The relationship between wing length and body weight of moths was examined based on the samples collected from three mountain areas in southern Korea, Mt. Hallasan (HL), Mt. Jirisan (JR), and Mt. Seungdalsan (MN) from June to October 2020. The study aimed to investigate the relationship between wing length and body weight and whether this relationship varied at the moth families and study sites. In addition, we investigated whether the body size depended on the elevation. We collected a total of 366 species and 1,356 individual moths across 17 families. The regression power for the combined taxa across all areas was 3.05 (±0.05 S.E.) and the regression power was high on the island mountain and this increased as the elevation rose. No significant association across the three mountains between body size and elevation, while the species-rich families such as Geometridae, Noctuidae, and Notodondidae showed different relationships across mountains.</p></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"27 3","pages":"Article 102294"},"PeriodicalIF":1.1,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141732183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A conserved amino acid replacement (A2′S) in RDL1 and multiple mutations in AChE1 were detected in the cotton aphid Aphis gossypii in Xinjiang China 在中国新疆棉蚜中发现 RDL1 的一个保守氨基酸置换(A2′S)和 AChE1 的多个突变
IF 1.1 3区 农林科学
Journal of Asia-pacific Entomology Pub Date : 2024-07-14 DOI: 10.1016/j.aspen.2024.102292
Otgonzaya Munkhbayar , Mei Li , Xinghui Qiu
{"title":"A conserved amino acid replacement (A2′S) in RDL1 and multiple mutations in AChE1 were detected in the cotton aphid Aphis gossypii in Xinjiang China","authors":"Otgonzaya Munkhbayar ,&nbsp;Mei Li ,&nbsp;Xinghui Qiu","doi":"10.1016/j.aspen.2024.102292","DOIUrl":"10.1016/j.aspen.2024.102292","url":null,"abstract":"<div><p>The cotton or melon aphid (<em>Aphis gossypii</em> Glover) is one of the most important agricultural insect pests worldwide. Many populations of this pest have been documented to be resistant to multiple classes of insecticides in Xinjiang Uygur Autonomous Region of China. Understanding the molecular mechanisms behind resistance in field populations is crucial for making effective strategies for control of this pest. In this study, we investigated potential genetic mutations responsible for insecticide resistance in two insecticidal targets in four cotton aphid populations in Xinjiang. Our data showed that the classical A2′S mutation in the γ-aminobutyric acid (GABA) receptor RDL1 was present in the cotton aphid at frequencies ranged from 4.5 % to 50 %, indicating that the cotton aphids have evolved the Rdl-based mechanism of resistance to synthetic insecticides via a parallel mutation in Rdl1 even in the genetic background with the 2ʹS-carrying duplicated Rdl (Rdl2). In addition, three mutations (A302S, A332V and S431F) in the acetylcholinesterase AChE1 were found to be prevalently distributed in <em>A. gossypii</em> populations. Notably, the resistant 431F allele was fixed in the four examined populations, and all existing <em>ace-1</em> alleles were resistant, carrying either one or two of the three mutations. The occurrence of resistance-conferring mutations in multiple insecticidal targets warns a risk of failure in aphid control using chemical insecticides in Xinjiang, the major cotton planting region of China.</p></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"27 3","pages":"Article 102292"},"PeriodicalIF":1.1,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141622236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A new species of the genus Elasmus Westwood (Hymenoptera: Eulophidae) parasitizing bagworm moth (Lepidoptera: Psychidae) 寄生于袋蛾(鳞翅目:灵猫科)的 Elasmus Westwood 属(膜翅目:绒毛虫科)新种
IF 1.1 3区 农林科学
Journal of Asia-pacific Entomology Pub Date : 2024-07-14 DOI: 10.1016/j.aspen.2024.102293
Jongmin Bae , Changmin Lee , Sangeun Hyun , Sanghyeok Yoon , Sieun Choi , Taejun Jeon , Sunghoon Jung
{"title":"A new species of the genus Elasmus Westwood (Hymenoptera: Eulophidae) parasitizing bagworm moth (Lepidoptera: Psychidae)","authors":"Jongmin Bae ,&nbsp;Changmin Lee ,&nbsp;Sangeun Hyun ,&nbsp;Sanghyeok Yoon ,&nbsp;Sieun Choi ,&nbsp;Taejun Jeon ,&nbsp;Sunghoon Jung","doi":"10.1016/j.aspen.2024.102293","DOIUrl":"10.1016/j.aspen.2024.102293","url":null,"abstract":"<div><p>In this study, we report a new species of the genus <em>Elasmus</em> Westwood (Hymenoptera: Eulophidae). The new species parasitizes two different unidentified Oiketicinae species of bagworm moths (Lepidoptera: Psychidae) and shows both solitary and gregarious traits. A description of the new species <em>Elasmus psycheae</em> <strong>n.sp.</strong> is provided in addition to figures and information about the biology. In addition, a key to the species of this genus from Korea is also presented.</p><p><span><span>www.zoobank.org/urn:lsid:zoobank.org:pub:17C2AA8B-4017-44BC-A44D-BD45746EC387</span><svg><path></path></svg></span>.</p></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"27 3","pages":"Article 102293"},"PeriodicalIF":1.1,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141716951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The first nymph-imago association and observed biology of the wood-dwelling mayfly Povilla heardi (Hubbard, 1984) from China (Ephemeroptera: Polymitarcyidae: Asthenopodinae) 中国木栖蜉蝣 Povilla heardi(Hubbard,1984 年)的首次若虫-子虫结合及观察到的生物学特性(蜉蝣目:多刺蜉蝣科:Asthenopodinae)
IF 1.1 3区 农林科学
Journal of Asia-pacific Entomology Pub Date : 2024-07-13 DOI: 10.1016/j.aspen.2024.102290
Xuhongyi Zheng , Pengxu Mu , Changfa Zhou
{"title":"The first nymph-imago association and observed biology of the wood-dwelling mayfly Povilla heardi (Hubbard, 1984) from China (Ephemeroptera: Polymitarcyidae: Asthenopodinae)","authors":"Xuhongyi Zheng ,&nbsp;Pengxu Mu ,&nbsp;Changfa Zhou","doi":"10.1016/j.aspen.2024.102290","DOIUrl":"https://doi.org/10.1016/j.aspen.2024.102290","url":null,"abstract":"<div><p>Although the species <em>Povilla heardi</em>Hubbard, 1984 was described from both males and putative nymphs, no photographic structures were presented, and this species was not found in China before. In 2023, dozens of adults were trapped and reared from nymphs in southwestern Chinese Yunnan Province. Detailed descriptions and photographs of them confirmed the synonym <em>Povilla junki</em> to <em>P. heardi</em>, and represent the first nymph-adult association of Asian species in the genus. In addition, our findings represent the first record of Asthenopodinae in this country, widened the Northern distribution of <em>Povilla</em>, and provide some biological information for this poorly known species.</p></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"27 3","pages":"Article 102290"},"PeriodicalIF":1.1,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141605758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
New species of Sinodima Kundrata, Sormova & Qiu, 2019 reveal the first case of strong sexual dimorphism in Dimini (Coleoptera: Elateridae) Sinodima Kundrata, Sormova & Qiu, 2019年的新种首次揭示了Dimini(鞘翅目:Elateridae)中强烈的性二型现象
IF 1.1 3区 农林科学
Journal of Asia-pacific Entomology Pub Date : 2024-07-10 DOI: 10.1016/j.aspen.2024.102289
Lu Qiu , Robin Kundrata
{"title":"New species of Sinodima Kundrata, Sormova & Qiu, 2019 reveal the first case of strong sexual dimorphism in Dimini (Coleoptera: Elateridae)","authors":"Lu Qiu ,&nbsp;Robin Kundrata","doi":"10.1016/j.aspen.2024.102289","DOIUrl":"10.1016/j.aspen.2024.102289","url":null,"abstract":"<div><p>ZooBank LSID: <span><span>urn:lsid:zoobank.org:pub:392A0D37-258B-4662-A318-BA671654E093</span><svg><path></path></svg></span>.</p><p>Genus <em>Sinodima</em> Kundrata, Sormova &amp; Qiu, 2019 was originally described based on a single species represented by the female holotype. In this study, we examined more than 500 specimens of <em>Sinodima</em> collected using Malaise traps. As a result, we described two new species from China: <em>Sinodima liupanshuiensis</em> sp. nov. and <em>Sinodima nanlingensis</em> sp. nov. The males of this genus are firstly reported and distinctly differentiated from the females by the elongate body and fully developed hind wings. Two new combinations are proposed for species originally described in genus <em>Penia</em> Laporte, 1838: <em>Sinodima elongata</em> (Arimoto, 2023), comb. nov. and <em>Sinodima sucinea</em> (Schimmel, 2001), comb. nov. The newly discovered <em>Sinodima</em> species provide the first report on strong sexual dimorphism in Dimini and highlight an unusual season activity of adults during autumn to early winter.</p></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"27 3","pages":"Article 102289"},"PeriodicalIF":1.1,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141636519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification and expression analysis of four sHsp genes in Sogatella furcifera under abiotic stress 鉴定和分析非生物胁迫下 Sogatella furcifera 中的四个 sHsp 基因
IF 1.1 3区 农林科学
Journal of Asia-pacific Entomology Pub Date : 2024-07-09 DOI: 10.1016/j.aspen.2024.102291
Tielong Xu , Cao Zhou
{"title":"Identification and expression analysis of four sHsp genes in Sogatella furcifera under abiotic stress","authors":"Tielong Xu ,&nbsp;Cao Zhou","doi":"10.1016/j.aspen.2024.102291","DOIUrl":"https://doi.org/10.1016/j.aspen.2024.102291","url":null,"abstract":"<div><p><em>Sogatella furcifera</em> is a typical r-strategist insect with strong adaptability and one of the major pests in rice cultivation. Small heat shock proteins (sHsp) play a vital role in insects responding to external abiotic stress. However, little is known about the role of <em>sHsp</em> in the defense against abiotic stress in <em>S. furcifera</em>. In this study, four <em>sHsp</em> genes (<em>SofHsp20.7</em>, <em>SofHsp21.6</em>, <em>SofHsp21.7</em>, and <em>SofHsp21.8</em>) were identified from <em>S. furcifera</em>, and their gene expression patterns were analyzed at different developmental stages, temperature stress, insecticide exposure, and UV-A irradiation. The results indicated that four <em>sHsp</em> were expressed at various developmental stages of the <em>S. furcifera</em>, with the highest transcription levels in the first-instar nymphs, fourth day fifth instar nymphs, third-day adult females, and eggs, respectively. High-temperature stress (30 °C and 40 °C) significantly induced the expression of all four <em>sHsp</em> genes, while low-temperature stress (10 °C) reduced their expression. Sublethal concentrations (LC<sub>10</sub> and LC<sub>25</sub>) of buprofezin increased the expression levels of <em>SofHsp20.7</em> and <em>SofHsp21.8</em>. Under UV-A irradiation, the <em>S. furcifera</em> also significantly induced the expression of all four <em>sHsp</em> genes, with the highest expression observed at 90 min after exposure to the stress. These results suggest that <em>sHsp</em> genes are essential for the resistance or tolerance of <em>S. furcifera</em> to high temperature, buprofezin, and UV-A irradiation. This research provides a foundation for understanding the molecular mechanisms underlying the <em>S. furcifera</em> adaptation to abiotic stress.</p></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"27 3","pages":"Article 102291"},"PeriodicalIF":1.1,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141606692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of two complete mitochondrial genomes of Aromia bungii Faldermann, 1835 (Coleoptera: Chrysomeloidea: Cerambycidae) Aromia bungii Faldermann, 1835 (Coleoptera: Chrysomeloidea: Cerambycidae) 两个完整线粒体基因组的特征分析
IF 1.1 3区 农林科学
Journal of Asia-pacific Entomology Pub Date : 2024-07-09 DOI: 10.1016/j.aspen.2024.102288
Hyobin Lee , Moo-Sung Kim , Jong-Kook Jung , Youngwoo Nam , Wonhoon Lee
{"title":"Characterization of two complete mitochondrial genomes of Aromia bungii Faldermann, 1835 (Coleoptera: Chrysomeloidea: Cerambycidae)","authors":"Hyobin Lee ,&nbsp;Moo-Sung Kim ,&nbsp;Jong-Kook Jung ,&nbsp;Youngwoo Nam ,&nbsp;Wonhoon Lee","doi":"10.1016/j.aspen.2024.102288","DOIUrl":"10.1016/j.aspen.2024.102288","url":null,"abstract":"<div><p>The red-necked longhorn beetle <em>Aromia bungii</em> (Coleoptera: Chrysomeloidea: Cerambycidae) is a wood-boring pest of <em>Prunus</em> spp. We analyzed two complete mitochondrial genomes of <em>A. bungii</em> collected in South Korea. The complete mitochondrial genomes were 15,759 bp and 15,760 bp long, including a typical set of genes (13 protein-coding, 22 transfer RNA, and two ribosomal RNA genes) and one non-coding region. Phylogenetic analysis revealed that the two mitochondrial genomes of <em>A. bungie</em> clustered with previously reported three mitochondrial genomes from China in one clade and was a sister group to <em>Closteromerus</em> belonging to the tribe Callichromatini.</p></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"27 3","pages":"Article 102288"},"PeriodicalIF":1.1,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141622235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Myocyte enhancer factor 2 exerts a pivotal role in larval development in Henosepilachna vigintioctopunctata 肌细胞增强因子 2 在雌梭鱼幼虫发育过程中发挥关键作用
IF 1.1 3区 农林科学
Journal of Asia-pacific Entomology Pub Date : 2024-07-07 DOI: 10.1016/j.aspen.2024.102287
Ahmad Ali Anjum, Meng-Jiao Lin, Lin Jin, Guo-Qing Li
{"title":"Myocyte enhancer factor 2 exerts a pivotal role in larval development in Henosepilachna vigintioctopunctata","authors":"Ahmad Ali Anjum,&nbsp;Meng-Jiao Lin,&nbsp;Lin Jin,&nbsp;Guo-Qing Li","doi":"10.1016/j.aspen.2024.102287","DOIUrl":"10.1016/j.aspen.2024.102287","url":null,"abstract":"<div><p>Myocyte enhancer factor-2 (MEF2) plays essential roles in a variety of developmental processes. Prominent amongst multiple MEF2 functions is the regulation of muscle differentiation in <em>Drosophila melanogaster</em>. Nevertheless, the physiological importance of MEF2 has not been explored in non-Drosophilid insect species. Here we found <em>HvMef2</em> was highly expressed at the first-instar larvae, 0-day-old second- and fourth-instar larvae, pupae and adults, especially in adult muscle in a Coleopteran potato defoliator <em>Henosepilachna vigintioctopunctata</em>. Injection of 200, 500 or 1000 ng of ds<em>Mef2</em> into the third instar larvae significantly reduced the expression of <em>HvMef2</em> and impaired larval-pupal-adult transition, in a dose-dependent manner. About 68 %, 77 % and 87 % of the resulting larvae arrested as stunted prepupae, which were gradually blackened and eventually died. Around 32 %, 23 % and 13 % of the <em>HvMef2</em> depleted beetles formed pupae. Among these <em>HvMef2</em> depleted pupae, 22 %, 16 % and 7 % became shriveled pupae until death; roughly 10 %, 7 % and 6 % emerged as malformed adults. The growth of the adult thorax muscles was repressed. The length and width of dorsal–ventral muscles were significantly reduced. Knockdown of <em>HvMef2</em> at the fourth larval instar stage caused similar defects. Likewise, introduction of ds<em>Mef2</em> at a dose of 200, 500 or 1000 ng into pupae led to 27 %, 20 % and 11 % emergence percentages respectively, with deformed elytra and hindwings. Therefore, MEF2 plays vital roles during metamorphosis, especially in myogenic regulation network in <em>H. vigintioctopunctata</em>.</p></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"27 3","pages":"Article 102287"},"PeriodicalIF":1.1,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141778690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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