Identification of candidate genes engaged in the sex-determination cascade in Eupolyphaga sinensis walker

IF 1.1 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology Pub Date : 2025-06-24 DOI:10.1016/j.aspen.2025.102434

Rui Zhu, Fei Yang, Tielong Xu

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Abstract

Eupolyphaga sinensis is a sexually dimorphic and an important medicinal insect in the East Asian traditional medicine. To deeply comprehend the sex-determination process underlying Eupolyphaga sinensis, this study conducted a large-scale transcriptomic analysis of its sex-determination genes. In total, 79,705 unigenes were derived via transcriptome sequencing, assembly, and annotation, and they had 836.30 bp and an N50 of 1422 bp. Putative sex-determination genes in E. sinensis, including doublesex (dsx), transformer-2, intersex, and fruitless, which are key ones often engaged in arthropod sex determination, were identified and characterized. For E. sinensis, a comparative network of protein–protein interactions for the sex-determination cascade was developed, using the network from the model insect Drosophila melanogaster as a reference. This research results offer a framework for determining target genes and comprehending the regulation of the sex-determination cascade in E. sinensis.

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中华白桦性别决定级联候选基因的鉴定

中华巨噬虫是一种两性异形昆虫，是东亚传统医学中重要的药用昆虫。为了深入了解中华多食蝗的性别决定过程，本研究对其性别决定基因进行了大规模的转录组学分析。通过转录组测序、组装和注释，共获得79,705个单基因，长度为836.30 bp， N50为1422 bp。对中华按蚊性别决定基因进行了鉴定和表征，包括双性（dsx）、转换-2、双性（intersex）和无果性（fruitless）等，它们是节肢动物性别决定的关键基因。以中华按蚊为研究对象，以黑腹果蝇（Drosophila melanogaster）的网络为参照，建立了一个蛋白质-蛋白质相互作用的性别决定级联比较网络。本研究结果为确定中华按蚊性别决定级联的靶基因和理解其调控机制提供了一个框架。

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来源期刊

Journal of Asia-pacific Entomology Agricultural and Biological Sciences-Insect Science

CiteScore

2.70

自引率

6.70%

发文量

152

审稿时长

69 days

期刊介绍： The journal publishes original research papers, review articles and short communications in the basic and applied area concerning insects, mites or other arthropods and nematodes of economic importance in agriculture, forestry, industry, human and animal health, and natural resource and environment management, and is the official journal of the Korean Society of Applied Entomology and the Taiwan Entomological Society.