Journal of Asia-pacific Entomology最新文献_第4页

Novel splice variants of the cytochrome P450 9A19 gene in the domestic silkworm Bombyx mori 家蚕细胞色素P450 9A19基因的新剪接变异

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-02-10 DOI: 10.1016/j.aspen.2025.102382

Jin Ha Yun, Seung-Won Park

{"title":"Novel splice variants of the cytochrome P450 9A19 gene in the domestic silkworm Bombyx mori","authors":"Jin Ha Yun, Seung-Won Park","doi":"10.1016/j.aspen.2025.102382","DOIUrl":"10.1016/j.aspen.2025.102382","url":null,"abstract":"<div><div><em>CYP9</em> gene family members participate in neutralization pathways associated with pesticide resistance. Alternative splicing ultimately plays a role in increasing the diversity of the proteome through increasing transcriptome diversity. Here, we characterized various novel <em>CYP9A19</em> isoforms in <em>Bombyx mori</em>. We first identified an exon 4 deletion isoform that was 203 bp smaller than expected due to a deletion of exon 4 from the protein coding sequence (CDS) region of the <em>CYP9A19</em> cDNA. Within the <em>CYP9A19</em> cDNA, there is a 176 bp 5′-untranslated region (UTR); reverse transcription-polymerase chain reaction results showed 74 bp lower and 122 bp higher molecular-weight products compared with the expected size from the 5′-UTR of <em>CYP9A19</em> cDNA in <em>B. mori</em>. Additionally, we found a new sequence (5′-GTCTCAGGTTCAGGGCTCTAGCAATTTTTCACAG-3′) in the 5′-UTR of <em>CYP9A19</em>. Currently, our understanding of the various <em>CYP9A19</em> isoform functions in pesticide degradation and resistance and insect development and differentiation stages remains limited. Therefore, additional studies on the alternative splicing process are needed to verify the biological role of the novel splicing variant of <em>CYP9A19</em> in <em>B. mori</em>. Our findings may provide insight into the genetic background of the processes and mechanisms underlying the stages of insect development and differentiation in lepidoptera species.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 1","pages":"Article 102382"},"PeriodicalIF":1.1,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two new species of the planthopper genus Pitambara Distant, 1906 (Hemiptera, Fulgoromorpha, Lophopidae) from Yunnan Province of China 云南飞虱属二新种（半翅目，飞虱科，飞虱科）

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-02-06 DOI: 10.1016/j.aspen.2025.102380

Shasha Lv , Lin Yang , Yubo Zhang , Xiangsheng Chen

引用次数: 0

A remarkable new species of the genus Dichodontus Burmeister, 1847 (Coleoptera: Scarabaeidae: Dynastinae) from Thailand and Myanmar, with discussions on some Sundaic species 泰国、缅甸双翅金龟属一显著新种，1847（鞘翅目：金龟科：金龟科），并对若干巽他亚种作了讨论

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-02-04 DOI: 10.1016/j.aspen.2025.102381

Georgi A. Geshev , Wuttipon Pathomwattananurak , Kawin Jiaranaisakul

引用次数: 0

Stethynium empoascae Subba Rao (Hymenoptera: Mymaridae), a newly recorded egg parasitoid of Amrasca biguttula (Ishida) (Hemiptera: Cicadellidae), a pest of okra on Ogasawara (Bonin) Islands, Japan Stethynium empoascae Subba Rao(膜翅目:Mymaridae),一个新记录鸡蛋的拟寄生物Amrasca biguttula(石田)(半翅类:叶蝉科),秋葵的害虫小笠原小笠原群岛,日本

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-01-23 DOI: 10.1016/j.aspen.2025.102379

Tetsuya Adachi-Hagimori , Serguei V. Triapitsyn , Takuya Endou

引用次数: 0

New complete mitogenomes of four species in tribe Erythroneurini (Hemiptera: Cicadellidae: Typhlocybinae) from China and their phylogenetic analysis 中国赤蝽科4种新的全有丝分裂基因组及其系统发育分析

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-01-22 DOI: 10.1016/j.aspen.2025.102378

Weiwei Ran , Yuanqi Zhao , Jiajia Chen , Ni Zhang , Zhouwei Yuan , Guimei Luo , Yuehua Song

{"title":"New complete mitogenomes of four species in tribe Erythroneurini (Hemiptera: Cicadellidae: Typhlocybinae) from China and their phylogenetic analysis","authors":"Weiwei Ran , Yuanqi Zhao , Jiajia Chen , Ni Zhang , Zhouwei Yuan , Guimei Luo , Yuehua Song","doi":"10.1016/j.aspen.2025.102378","DOIUrl":"10.1016/j.aspen.2025.102378","url":null,"abstract":"<div><div>Erythroneurine leafhoppers are important insects in agriculture and forestry. The complete mitochondrial genomes of <em>Motaga mengyangensis</em>, <em>Motaga acicularis</em>, <em>Tautoneura albida</em>, and <em>Tautoneura unicolor</em>, which belong to the tribe Erythroneurini from China, were sequenced for the first time. The lengths of the complete mitogenomes of these four species ranged from 15,361 to 15,856 base pairs, and a typical set of 37 genes was identified in each. Among the 13 protein-coding genes (PCGs), except for the <em>atp8</em> gene in <em>T. albida and T. unicolor</em>, which started with the codon TTG, the remaining genes all began with typical ATN codons (ATA/ATT/ATC/ATG). The stop codons were TAA, TAG, or an incomplete stop codon T. Utilizing three datasets from the mitochondrial genomes (PCGs, PCGsrRNA, and PCGs12rRNA) of 36 species (including two outgroups), phylogenetic trees of Erythroneurini were constructed via maximum likelihood and Bayesian inference methods. The results revealed that all phylogenetic trees supported the clustering of <em>M. mengyangensis</em> and <em>M. acicularis</em> into one evolutionary branch, as well as the clustering of <em>T. albida</em> and <em>T. unicolor</em> into another. Furthermore, all the results consistently supported the monophyly of genera within Erythroneurini and uniformly demonstrated that the genus <em>Motaga</em> formed a sister group relationship with the genus <em>Empoascanara</em>. Our research fills a crucial gap in the available data on complete mitochondrial genomes within Erythroneurini, laying a solid foundation for future explorations into the molecular aspects of leafhopper evolution and diversity, which further provides reference value for their prevention and control.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 1","pages":"Article 102378"},"PeriodicalIF":1.1,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The genus Deutereulophus Schulz, 1906 (Hymenoptera: Eulophidae: Eulophinae) with description of three new species from Indonesia 印度尼西亚细叶蜂属，1906（膜翅目：细叶蜂科：细叶蜂科）及三新种记述

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-01-20 DOI: 10.1016/j.aspen.2025.102377

Rosichon Ubaidillah , Encilia Encilia , Darmawan Darmawan , Hari Nugroho , Hari Sutrisno , Jun-ichi Kojima

引用次数: 0

LC-MS profiling reveals metabolic dynamics in Apis mellifera worker bee larvae–pupae transition 液相色谱-质谱分析揭示了蜜蜂幼虫-蛹转化过程中的代谢动力学

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-01-16 DOI: 10.1016/j.aspen.2025.102376

Zhen Li , Shiqing Zhong , Bin Liu , Lizhi Li , Haibo Zhang , Weikun Guan , Dongsheng Guo

{"title":"LC-MS profiling reveals metabolic dynamics in Apis mellifera worker bee larvae–pupae transition","authors":"Zhen Li , Shiqing Zhong , Bin Liu , Lizhi Li , Haibo Zhang , Weikun Guan , Dongsheng Guo","doi":"10.1016/j.aspen.2025.102376","DOIUrl":"10.1016/j.aspen.2025.102376","url":null,"abstract":"<div><div>Metabolites present in the hemolymph of honey bees play a crucial role in modulating the metamorphic process within the species. However, the precise alterations in metabolite composition, along with the associated variances and regulatory pathways implicated during the larvae-to-pupae metamorphosis of honey bees, remain incompletely elucidated. In this investigation, we gathered hemolymph samples from honey bee larvae of <em>Apis mellifera</em> at three distinct physiological stages-feeding, prepupae, and pupae and subjected them to metabolite analysis utilizing the liquid chromatography-mass spectrometry (LC-MS) technique. Employing principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA), we identified significant differential metabolites and conducted a metabolic pathway analysis on those differentially up-regulated during the prepupae and pupae stages. Notably, metabolites up-regulated in the hemolymph of prepupae stage larvae primarily governed glucose metabolism and fat digestion and absorption, while those in pupae stage were involved in regulating chitin and lipopolysaccharide precursor formation, as well as the biosynthesis of phenylalanine, tyrosine, and tryptophan. These findings bear significant implications for advancing our comprehension of the metamorphic processes in honey bees.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 1","pages":"Article 102376"},"PeriodicalIF":1.1,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Description of three new species of Trigonidiidae Saussure, 1874 from China (Orthoptera: Grylloidea) 中国三角蝽科三新种记述（直翅目：灰蝇总科）

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-01-15 DOI: 10.1016/j.aspen.2025.102372

Tao Li-Long , Yang Rui-Ying , He Zhi-Xin , Ma Li-Bin

{"title":"Description of three new species of Trigonidiidae Saussure, 1874 from China (Orthoptera: Grylloidea)","authors":"Tao Li-Long , Yang Rui-Ying , He Zhi-Xin , Ma Li-Bin","doi":"10.1016/j.aspen.2025.102372","DOIUrl":"10.1016/j.aspen.2025.102372","url":null,"abstract":"<div><div>The article describes three new species of Nemobiinae Saussure, 1877 and Trigonidiinae Saussure, 1874 from Yunnan Province, namely <em>Pteronemobius (Stilbonemobius) alboguttatus</em> Ma & Tao <strong>sp. nov.</strong>, <em>Metiochodes wanzhii</em> Ma & Tao <strong>sp. nov.</strong> and <em>Metiochodes polycomus</em> Ma & Tao <strong>sp. nov.</strong>. Among them, <em>P. (S.) alboguttatus</em> <strong>sp. nov.</strong> has a short, rounded shield on the ectoparamere, four internal dorsal spurs on the hind tibia, and many white spots on the head, other morphological characteristics are similar to <em>Pteronemobius (Stilbonemobius) longispinus</em> Gorochov, 1984. Furturemore, <em>M. wanzhii</em> <strong>sp. nov.</strong> and <em>M. polycomus</em> <strong>sp. nov.</strong> can be distinguished by the number of main veins in the forewing and the shape of the epiphallus and ectoparamere. Here, we provide a detailed description and photos of the new species.</div><div>LSIDurn:lsid:<span><span>zoobank.org</span><svg><path></path></svg></span>:pub:099A8DC4-0D79-4390-AC28-44E14899EFD8.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 1","pages":"Article 102372"},"PeriodicalIF":1.1,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A deep learning-based method for silkworm egg counting 一种基于深度学习的蚕卵计数方法

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-01-11 DOI: 10.1016/j.aspen.2025.102375

Hongkang Shi, Xiao Chen, Minghui Zhu, Linbo Li, Jianmei Wu, Jianfei Zhang

{"title":"A deep learning-based method for silkworm egg counting","authors":"Hongkang Shi, Xiao Chen, Minghui Zhu, Linbo Li, Jianmei Wu, Jianfei Zhang","doi":"10.1016/j.aspen.2025.102375","DOIUrl":"10.1016/j.aspen.2025.102375","url":null,"abstract":"<div><div>The counting of silkworm eggs is an essential task in the selection and breeding of new silkworm species, as well as in silkworm egg production. Currently, this task mainly relies on manual counting, which poses many challenges such as high workload, low efficiency, and being error-prone. To alleviate these problems, this study proposes a deep learning-based method for silkworm egg counting. Images of silkworm eggs were captured from actual environments and annotated using a labeling tool, resulting in more than 300,000 labeled eggs. A counting network based on the You Only Look Once (YOLOv8n) object detection network is proposed, in which an efficient multi-scale attention (EMA) module is embedded in the extraction block of the original network to enhance feature representation capability and suppress interference. To further improve counting performance, a space-to-depth convolution (SPD-Conv) block is introduced to replace the down-sample layer implemented by convolutional layers with a stride of 2. The proposed network is termed YOLO for silkworm egg counting (YOLO-SEC). Experimental results demonstrate that our YOLO-SEC achieves a recall of 99.50 %, a precision of 98.29 %, an F1-score of 0.99, and an AP of 99.31 % for silkworm eggs on the test set. Meanwhile, YOLO-SEC shows significant performance advantages over the original YOLOv8 (n ∼ x), state-of-the-art networks (YOLOv7-tiny, YOLOv9s, YOLOv10n and YOLOv11n), and related networks (YOLOv8-QR, EDGC-YOLO, Faster-YOLO-AP, YOLOv8-ECFS). This research provides technical support for the breeding and egg production process of silkworms.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 1","pages":"Article 102375"},"PeriodicalIF":1.1,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143150174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host-associated genetic analysis of Thrips hawaiiensis (Thysanoptera: Thripidae), inferred from mitochondrial and microsatellite data 基于线粒体和微卫星数据的夏威夷蓟马（Thysanoptera: Thripidae）宿主相关遗传分析

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-01-11 DOI: 10.1016/j.aspen.2025.102374

Haixin Qin , Chennan Yu , Chaorong Meng , Xue Cen , Maofa Yang , Shimeng Zhang

{"title":"Host-associated genetic analysis of Thrips hawaiiensis (Thysanoptera: Thripidae), inferred from mitochondrial and microsatellite data","authors":"Haixin Qin , Chennan Yu , Chaorong Meng , Xue Cen , Maofa Yang , Shimeng Zhang","doi":"10.1016/j.aspen.2025.102374","DOIUrl":"10.1016/j.aspen.2025.102374","url":null,"abstract":"<div><div><em>Thrips hawaiiensis</em> (Morgan, 1913) (Thysanoptera, Thripidae), a frequent pest in crops and horticultural plants, is widely distributed in China. However, the population evolutionary history and genetic variability of <em>T. hawaiiensis</em> is unknown. In the current study, the genetic diversity and structure of <em>T. hawaiiensis</em> from thirteen host plants in two regions were detected using mitochondrial cytochrome oxidase subunit I (COI) gene and six microsatellite loci. From 123 individuals collected from thirteen flowering hosts, 23 haplotypes were identified, and the high genetic diversity of <em>T. hawaiiensis</em> was demonstrated by COI sequence and microsatellite data. Haplotype analysis indicated two clusters of <em>T. hawaiiensis</em> populations, Bayesian clustering analysis and PCoA analysis also supported this result. The genetic differentiation coefficient (F<sub>st</sub>) and gene flow (Nm) showed weak gene differentiation and relatively high gene flow in <em>T. hawaiiensis</em> from different hosts. This might be facilitated through human activities, especially CL and GYZYMRJ with the closest geographical distance. The results of demographic analysis suggested that the differences in genetic structure of <em>T. hawaiiensis</em> from different host plants are not obvious, and genetic changes may be related to pesticide stres. The possible influences of <em>T. hawaiiensis</em> invasion history and human activities on the current haplotype host distribution were interpreted.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 1","pages":"Article 102374"},"PeriodicalIF":1.1,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143150054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0