Journal of Asia-pacific Entomology最新文献_第4页

The mechanism of high-concentration florfenicol exposure inducing apoptosis in the midgut of Bombyx mori (Lepidoptera: Bombycidae) 高浓度氟苯尼考诱导家蚕中肠细胞凋亡的机制（鳞翅目：家蚕科）

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-03-20 DOI: 10.1016/j.aspen.2025.102399

Chao Tian , Jialu Cheng , Haoyi Gu , Hongbin Zou , Peilin Peng , Bing Li

{"title":"The mechanism of high-concentration florfenicol exposure inducing apoptosis in the midgut of Bombyx mori (Lepidoptera: Bombycidae)","authors":"Chao Tian , Jialu Cheng , Haoyi Gu , Hongbin Zou , Peilin Peng , Bing Li","doi":"10.1016/j.aspen.2025.102399","DOIUrl":"10.1016/j.aspen.2025.102399","url":null,"abstract":"<div><div>The silkworm (<em>Bombyx mori</em>) is a Lepidoptera model insect, high concentrations of FF can impair the midgut physiological function, but the mechanism of action of which is still unclear. In this study, silkworms were continuously exposed to high concentrations (1.2 g/L) of FF for 72 h to investigate its mechanism of action. The results showed that FF exposure significantly inhibited the activity of key antioxidant enzymes, SOD, CAT, and TPX, in the midgut. Transmission electron microscopy revealed notable cellular damage, including microvilli shedding, chromatin aggregation, nuclear membrane depression, double membrane-bound vesicle autophagosomes, mitochondrial membrane rupture and swelling in the midgut after FF exposure. Furthermore, the oxidative phosphorylation pathway in the midgut was markedly suppressed, and the release of mitochondrial BmCytochrome c was significantly increased in the treatment group. The protein expression levels of BmCaspase-3 and cleaved BmCaspase-3 significantly were upregulated by 2.35-fold and 1.33-fold respectively, indicating the activation of the apoptotic signaling pathway. Additionally, TUNEL staining confirmed increased apoptotic signals in the midgut induced by FF. These findings suggest that high-concentration FF exposure triggers midgut apoptosis by reactive oxygen species (ROS) accumulation, which is the mechanism of physiological dysfunction in the midgut of silkworms. The research results provide a reference for evaluating the safety of high-concentration FF exposure in non-target organisms.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 2","pages":"Article 102399"},"PeriodicalIF":1.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143688015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systematic position of marine littoral Pseudothinusa Tasaku and Maruyama (Coleoptera: Staphylinidae: Aleocharinae) based on morphological and molecular evidence 基于形态学和分子证据的海洋滨海拟藻的系统位置（鞘翅目：拟藻科：拟藻科）

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-03-20 DOI: 10.1016/j.aspen.2025.102401

Jong-Keun Jung , Yuto Tasaku , Kee-Jeong Ahn

{"title":"Systematic position of marine littoral Pseudothinusa Tasaku and Maruyama (Coleoptera: Staphylinidae: Aleocharinae) based on morphological and molecular evidence","authors":"Jong-Keun Jung , Yuto Tasaku , Kee-Jeong Ahn","doi":"10.1016/j.aspen.2025.102401","DOIUrl":"10.1016/j.aspen.2025.102401","url":null,"abstract":"<div><div>A systematic study of the coastal Athetini monotypic genus <em>Pseudothinusa</em> Tasaku and Maruyama is presented using morphological and molecular characters. Detailed examination of the voucher specimens of <em>P. sunahama</em> Tasaku and Maruyama revealed that they represent a species of <em>Adota</em> Casey based on the following combination of characters: body surface with isodiametric microsculpture; antennomere 2 longer than 3; medial lamellae of internal sac of median lobe of aedeagus reduced; spermathecal duct coiled once distally. The proposed synonymy of <em>Pseudothinusa</em> under <em>Adota</em> was also supported by gene tree criterion (PA, BA, and ML trees) using partial <em>COI</em> and <em>28S</em> gene sequences. This new synonymy (<em>Adota</em> <span><span>Casey, 1910</span></span> = <em>Pseudothinusa</em> <span><span>Tasaku and Maruyama, 2023</span></span>, <strong>new syn.</strong>) necessitates the following new combination: <em>Adota sunahama</em> (Tasaku and Maruyama), <strong>new comb.</strong> We also provide scanning electron microscopy (SEM) micrographs for their diagnostic characters. A checklist of <em>Adota</em> from the world and a key to species of <em>Adota</em> are presented.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 2","pages":"Article 102401"},"PeriodicalIF":1.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143688014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Definition of the Ichthyurus feae species group (Cantharidae, Chauliognathinae, Ichthyurini), with descriptions of three new species from China 标题鱼龙科（斑马鱼科）种群定义及中国三新种描述

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-03-19 DOI: 10.1016/j.aspen.2025.102400

Hanqing Lin , Haoyu Liu , Xingke Yang , Yuxia Yang

{"title":"Definition of the Ichthyurus feae species group (Cantharidae, Chauliognathinae, Ichthyurini), with descriptions of three new species from China","authors":"Hanqing Lin , Haoyu Liu , Xingke Yang , Yuxia Yang","doi":"10.1016/j.aspen.2025.102400","DOIUrl":"10.1016/j.aspen.2025.102400","url":null,"abstract":"<div><div>A cantharid species group consisting of the species morphologically similar to <em>Ichthyurus feae</em> <span><span>Gestro, 1891</span></span> is defined. The <em>I. feae</em> species-group is characterized by the middle-sized and relatively stout body, mesofemora moderately or strongly swollen and with several denticles in male, while normal in female; terminal abdominal tergite strongly ridged along ventro-lateral margins in male, with slender and horn-shaped lateral projections in both sexes; terminal abdominal ventrite greatly reduced in male, with proctiger and paraproct completely exposed, while well developed and rounded, dentated at posterior margin in female. It contains a total of nine species, including <em>I. feae</em> <span><span>Gestro, 1891</span></span> (=<em>I. lii</em> <strong>nom. nov.</strong> for <em>I. laosensis</em> <span><span>Li et al., 2014</span></span> <strong>syn. nov.</strong>), <em>I. longicauda</em> <span><span>Gestro, 1891</span></span>, <em>I. nigromaculatus</em> <span><span>Gestro, 1891</span></span>, <em>I. quadrimaculatus</em> <span><span>Gestro, 1888</span></span>, <em>I. melanospilus</em> <span><span>Gestro, 1892</span></span>, <em>I. salvazai</em> <span><span>Pic, 1943</span></span> (=<em>I. bernardi</em> Li et Gao, 2015 <strong>syn. nov.</strong>), <em>I. gracilicauda</em> <strong>sp. nov.</strong>, <em>I. multidentatus</em> <strong>sp. nov.</strong> and <em>I. hainanensis</em> <strong>sp. nov.</strong> The new species are all discovered from China, and <em>I. feae</em> and <em>I. salvazai</em> are firstly recorded to China and redescribed. These species are illustrated with habitus, genital segments of both sexes, aedeagi and internal genitalia of females. In addition, a distribution map and an identification key to all species of the <em>I. feae</em> species-group are provided.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 2","pages":"Article 102400"},"PeriodicalIF":1.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143688013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metagenomic approaches reveal the variations of microbiota structures and functions with the growth of Melanaphis sacchari 宏基因组学方法揭示了糖黑蚜生长过程中微生物群结构和功能的变化

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-03-18 DOI: 10.1016/j.aspen.2025.102402

Yunchao Wang , Jingjing Li , Tinghui Wu , Yinwei Ruan , Zhiyuan Ning , Yanhui Lu , Jiawen Guo , Zhongjiu Xiao , Kun Yang

{"title":"Metagenomic approaches reveal the variations of microbiota structures and functions with the growth of Melanaphis sacchari","authors":"Yunchao Wang , Jingjing Li , Tinghui Wu , Yinwei Ruan , Zhiyuan Ning , Yanhui Lu , Jiawen Guo , Zhongjiu Xiao , Kun Yang","doi":"10.1016/j.aspen.2025.102402","DOIUrl":"10.1016/j.aspen.2025.102402","url":null,"abstract":"<div><div>Melanaphis sacchari, commonly known as the sugarcane aphid, is a small sap-sucking insect that transmits several plant viruses, including Sugarcane mosaic virus and Sugarcane yellow leaf virus, both of which result in reduced crop yield and quality. This study explores the microbiota structure and function in different developmental stages of M. sacchari by using full-length 16S rRNA gene sequencing. Alpha diversity indices were used to compare microbiota diversity across these stages. Key bacteria, such as Buchnera aphidicola, Burkholderia stabilis, and Paenibacillus pasadenensis, were identified and quantified, showing that both diversity and abundance of bacterial communities vary across aphid stages. Buchnera aphidicola is most abundant in egg period compared to other stages. Functional predictions based on the KEGG database indicated that most bacterial genes are associated with metabolism. This study provides a foundational understanding of the bacterial microbiota in M. sacchari, enhancing insights into the complex interactions between microbiota and host insects and highlighting the essential role of microbiota in M. sacchari development.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 2","pages":"Article 102402"},"PeriodicalIF":1.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel ciprofloxacin-degrading bacteria Phytobacter diazotrophicus SMX isolated from silkworm faeces: characteristics, degradation metabolites, and toxicity assessment 从家蚕粪便中分离的新型环丙沙星降解细菌重氮营养菌SMX：特性、降解代谢物和毒性评估

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-03-13 DOI: 10.1016/j.aspen.2025.102392

Jia Kong , Xueping Jiang , Yuqi Li , Jielin Zhou , Ran Zhang , Hao Li , Zhongzheng Gui

{"title":"A novel ciprofloxacin-degrading bacteria Phytobacter diazotrophicus SMX isolated from silkworm faeces: characteristics, degradation metabolites, and toxicity assessment","authors":"Jia Kong , Xueping Jiang , Yuqi Li , Jielin Zhou , Ran Zhang , Hao Li , Zhongzheng Gui","doi":"10.1016/j.aspen.2025.102392","DOIUrl":"10.1016/j.aspen.2025.102392","url":null,"abstract":"<div><div>Ciprofloxacin (CIP), a feed additive commonly used in the aquaculture industry, tends to persist in the environment, potentially causing pollution. In this study, a novel CIP-degrading bacterium, designated SMX, was isolated from fermented silkworm excrement and identified by whole-genome sequencing. The antibiotic degradation efficiency was assessed using high-performance liquid chromatography (HPLC), and the degradation conditions were optimized. The degradation products were identified using ultra-high-performance liquid chromatography-mass spectrometry (UPLC-QTOF-MS/MS), and their toxicity was evaluated using the ecological structure–activity relationship (ECOSAR) program. The findings revealed that the CIP-degrading bacterium SMX belongs to <em>Phytobacter diazotrophicus</em>. The optimal conditions for CIP degradation by strain SMX were as follows: initial CIP concentration, 2.5 mg/L; temperature, 30 °C; and pH 8.0, with glucose as the primary external carbon source. The highest degradation efficiency of CIP by <em>Phytobacter diazotrophicus</em> SMX was 87.37% after 72 h. Four intermediate metabolites (IP1, IP2, IP3, and IP4) were identified. These metabolites had no toxic effect on the environment. In summary, SMX might be an efficient and safe CIP-degrading bacterium, poised to play a significant role in preventing and mitigating antibiotic cross-contamination.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 2","pages":"Article 102392"},"PeriodicalIF":1.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143807408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential electrophysiological responses of wingless and winged forms of three non-host-alternating aphids to plant volatile compounds 无翅和有翅三种非寄主交替蚜虫对植物挥发性化合物的不同电生理反应

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-03-07 DOI: 10.1016/j.aspen.2025.102395

Ya-Ya Li , Jinyue Qiao , Jicheng Wang , Xiujie Jia , Yuting Tang , Kairu Hou , Deguang Liu , Li Chen

{"title":"Differential electrophysiological responses of wingless and winged forms of three non-host-alternating aphids to plant volatile compounds","authors":"Ya-Ya Li , Jinyue Qiao , Jicheng Wang , Xiujie Jia , Yuting Tang , Kairu Hou , Deguang Liu , Li Chen","doi":"10.1016/j.aspen.2025.102395","DOIUrl":"10.1016/j.aspen.2025.102395","url":null,"abstract":"<div><div>From spring to autumn, aphids produce two types of asexual morphs: wingless and winged. Olfactory cues are often utilized by the winged morph in long-distance host plant selection, and by the wingless morph in short-range host plant location. Electroantennogram (EAG) responses to a range of plant volatiles were recorded from wingless and winged forms of the English grain aphid <em>Sitobion avenae</em>, greenbug <em>Schizaphis graminum</em>, and pea aphid <em>Acyrthosiphon pisum</em>. The response patterns of the three aphid species were similar, although some variations did occur. (<em>E</em>)-2-Hexenal elicited the largest EAG responses in both asexual forms (i.e., wingless and winged) of the three species. In general, green leaf volatiles and benzaldehyde produced relatively large EAGs. The winged form was more responsive than the wingless form to all the plant volatile compounds tested but 4-ethylbenzaldehyde for <em>S. graminum</em>, and hexanal and benzyl acetate for <em>A. pisum</em>. This study indicates that both forms of <em>S. avenae</em>, <em>S. graminum</em>, and <em>A. pisum</em> are able to detect various volatile compounds, and generally higher EAG response in winged forms suggest that they have a more responsive olfactory system than wingless forms, and are better-adapted for switching host plants under unfavorable environmental conditions.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 2","pages":"Article 102395"},"PeriodicalIF":1.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new species and new records with a key to South Korean moth flies (Diptera: Psychodidae: Psychodinae) 韩国飞蛾一新种及新记录（双翅目：飞蛾科：飞蛾科）

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-03-06 DOI: 10.1016/j.aspen.2025.102394

Sang-Woo Kim , Sam-Kyu Kim

引用次数: 0

Transcriptomic analysis of Drosophila melanogaster larvae exposed to dimethyl phthalate reveals molecular insights into developmental retardation 接触邻苯二甲酸二甲酯的黑腹果蝇幼虫转录组学分析揭示了发育迟缓的分子机制

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-03-05 DOI: 10.1016/j.aspen.2025.102391

Dan He , Jinxu Liu , Weihui Xu , Yunlong Hu , Wenjing Chen , Zhenyu Fan , Zhigang Wang

{"title":"Transcriptomic analysis of Drosophila melanogaster larvae exposed to dimethyl phthalate reveals molecular insights into developmental retardation","authors":"Dan He , Jinxu Liu , Weihui Xu , Yunlong Hu , Wenjing Chen , Zhenyu Fan , Zhigang Wang","doi":"10.1016/j.aspen.2025.102391","DOIUrl":"10.1016/j.aspen.2025.102391","url":null,"abstract":"<div><div>Dimethyl phthalate (DMP), a ubiquitous environmental pollutant, has been recognized for its capacity to impede biological development. Therefore, it is crucial to understand the mechanism by which DMP inhibits biological processes. This study employed the <em>Drosophila melanogaster</em> larvae as a model organism to explore the mechanisms of developmental retardation induced by DMP exposure via a transcriptomic approach. The results showed that 1.2 g/L DMP exposure induced developmental retardation in larvae, as evidenced by a significant increase in the larval-to-pupal transition time compared to the standard diet. Furthermore, transcriptome sequencing revealed 4451 differentially expressed genes (DEGs) in DMP-exposed larvae, comprising 2430 up-regulated and 394 down-regulated genes. These DEGs were predominantly involved in pathways such as carbohydrate metabolism, signal transduction, transporter metabolism, endocrine system, and tumorigenesis. This indicates that developmental toxicity associated with DMP exposure is intricately linked to these pathways, underscoring the need to address the detrimental effects of DMP exposure. To summarize, exposure to DMP at a specific concentration has a deleterious effect on organismal development.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 2","pages":"Article 102391"},"PeriodicalIF":1.1,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infection of tomato yellow leaf curl virus changes metabolites of the whitefly, Bemisia tabaci 侵染番茄黄卷叶病毒可改变粉虱的代谢物

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-03-05 DOI: 10.1016/j.aspen.2025.102393

Junbeom Lee , Dae-Weon Lee

引用次数: 0

Molecular identification and functional analysis of the putative ecdysone receptor in Spodoptera litura 斜纹夜蛾蜕皮激素受体的分子鉴定及功能分析

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-03-01 DOI: 10.1016/j.aspen.2025.102390

Ziqian Tong , Huixuan Shi , Zhuo Liu , Donghai Zhang , Dandan Li , Yunchao Kan , Huili Qiao

{"title":"Molecular identification and functional analysis of the putative ecdysone receptor in Spodoptera litura","authors":"Ziqian Tong , Huixuan Shi , Zhuo Liu , Donghai Zhang , Dandan Li , Yunchao Kan , Huili Qiao","doi":"10.1016/j.aspen.2025.102390","DOIUrl":"10.1016/j.aspen.2025.102390","url":null,"abstract":"<div><div>20-hydroxyedysone (20E) is an important hormone that regulates the process of molting and metamorphosis in insects. Ecdysone receptor (EcR) is one of the most important primary nuclear receptor for 20E. To clarify the essential function of EcR during the development of <em>Spodoptera litura</em>, the gene encoding the putative ecdysone receptor isoform A (<em>SlEcRA</em>) was cloned and verified. Multiple sequence comparisons and the subsequent phylogenetic study indicated that SlEcRA protein exhibited a high degree of conservation and significant homology with EcRA proteins in other insects. Spatiotemporal expression analysis showed that <em>SlEcRs</em> were predominantly expressed during the larval molting and prepupa stages, with the highest expression in fat body. Moreover, the expression of <em>SlEcRs</em> were induced by 20E in fat body, midgut and integument. Silencing of <em>SlEcRs</em> led to increased mortality and decreased larval weight during larval development. A combination treatment of dsSlEcRs and low-concentration emamectin benzoate effectively increased larvae mortality at 48 h. Furthermore, the larval mortality of Chi-dsSlEcRs nanoparticles by epidermal infiltration was much higher than the naked dsSlEcRs by injection. Co-delivery of Chi-dsSlEcRs and emamectin benzoate exhibited shorter action time and higher mortality rate on larvae. These findings not only highlight the potential of the putative <em>SlEcR</em> as an RNA interference target for <em>S. litura</em> control, but also provide a new idea for reducing the reliance on chemical insecticides in agricultural pest control.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 1","pages":"Article 102390"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0