Journal of Biomedicine and Biotechnology最新文献_第8页

Factors of the lectin pathway of complement activation and their clinical associations in neonates. 新生儿补体激活凝集素途径的影响因素及其临床相关性。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-03-22 DOI: 10.1155/2012/363246

Maciej Cedzynski, Anna St Swierzko, David C Kilpatrick

{"title":"Factors of the lectin pathway of complement activation and their clinical associations in neonates.","authors":"Maciej Cedzynski, Anna St Swierzko, David C Kilpatrick","doi":"10.1155/2012/363246","DOIUrl":"https://doi.org/10.1155/2012/363246","url":null,"abstract":"This paper summarizes the data concerning soluble defense lectins (mannan-binding lectin, M-ficolin, L-ficolin, and H-ficolin) with the unique ability to activate complement and their associated serine proteases (MASPs) in neonates. The clinical importance of deficiencies of these immune factors is presented in aspects of perinatal mortality, premature births, and low birthweight. Prenatal serum concentrations of L-ficolin, H-ficolin, and MASP-2 (and probably M-ficolin) correlate with gestational age and birthweight. The relationship of serum MBL to gestational age is controversial. The MBL2 genotypes XA/O and O/O (associated with low-serum MBL) are associated with perinatal infections, whereas the high serum MBL-conferring A/A genotypes may be associated with prematurity. Low-serum L-ficolin concentrations, but not low-serum H-ficolin concentrations, are also associated with perinatal infections. Much of the literature is inconsistent, and the relationships reported so far require independent confirmation at both gene and protein levels. Our preliminary conclusion is that these soluble defense lectins play a protective role in the neonate, and that insufficiency of such factors contributes to the adverse consequences of prematurity and low birthweight.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"363246"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/363246","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30637576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Protection of SH-SY5Y neuronal cells from glutamate-induced apoptosis by 3,6'-disinapoyl sucrose, a bioactive compound isolated from Radix Polygala. 3,6′-二氨基糖对谷氨酸诱导的SH-SY5Y神经元细胞凋亡的保护作用

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2011-06-30 DOI: 10.1155/2012/728342

Yuan Hu, Jie Li, Ping Liu, Xu Chen, Dai-Hong Guo, Qing-Shan Li, Khalid Rahman

{"title":"Protection of SH-SY5Y neuronal cells from glutamate-induced apoptosis by 3,6'-disinapoyl sucrose, a bioactive compound isolated from Radix Polygala.","authors":"Yuan Hu, Jie Li, Ping Liu, Xu Chen, Dai-Hong Guo, Qing-Shan Li, Khalid Rahman","doi":"10.1155/2012/728342","DOIUrl":"https://doi.org/10.1155/2012/728342","url":null,"abstract":"The neuroprotective effects of 3,6'-disinapoyl sucrose (DISS) from Radix Polygala against glutamate-induced SH-SY5Y neuronal cells injury were evaluated in the present study. SH-SY5Y neuronal cells were pretreated with glutamate (8 mM) for 30 min followed by cotreatment with DISS for 12 h. Cell viability was determined by (3,4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazolium bromide (MTT) assay, and apoptosis was confirmed by cell morphology and flow cytometry assay, evaluated with propidium iodide dye. Treatment with DISS (0.6, 6, and 60 μmol/L) increased cell viability dose dependently, inhibited LDH release, and attenuated apoptosis. The mechanisms by which DISS protected neuron cells from glutamate-induced excitotoxicity included the downregulation of proapoptotic gene Bax and the upregulation of antiapoptotic gene Bcl-2. The present findings indicated that DISS exerts neuroprotective effects against glutamate toxicity, which might be of importance and contribute to its clinical efficacy for the treatment of neurodegenerative diseases.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/728342","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29928938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 285

Comment on "Emerging functions of transcription factors in malaria parasite". 关于“转录因子在疟原虫中的新功能”的评论。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-05-20 DOI: 10.1155/2012/754054

Heather J Painter, Manuel Llinás

{"title":"Comment on \"Emerging functions of transcription factors in malaria parasite\".","authors":"Heather J Painter, Manuel Llinás","doi":"10.1155/2012/754054","DOIUrl":"https://doi.org/10.1155/2012/754054","url":null,"abstract":"In a recent Journal of Biomedicine and Biotechnology special issue on Immunology and Cell Biology of Parasitic Disease (2011), Tuteja et al. [1] authored a review summarizing transcription factors in the malaria parasite, Plasmodium. It is well known that there are very few characterized transcriptional regulators in the malaria parasite [2–4]. To date, the sole family of transcriptional regulators in Plasmodium consists of a conserved group of proteins containing a DNA-interaction domain with high homology to the Arabidopsis APETELA2 (AP2) DNA-binding domain [5]. Related AP2-integrase DNA-binding domains are also present in various Tetrahymena species, a few viruses, and cyanobacteria (reviewed in [6]). However, there have been no reports of an AP2 expansion in any other eukaryote other than the Apicomplexans. This lineage-specific expansion is now known as the Apicomplexan AP2 (ApiAP2) protein family [5], and since these proteins represent the first family of putative specific transcriptional regulators in the malaria parasite, their characterization has generated a flurry of recent reports [7–10]. The authors of this review, unfortunately, report an incorrect association between the ApiAP2 proteins (pfam PF00847) and the Activator Protein-2 (AP-2) (pfam PF03299) found in higher eukaryotes (reviewed in [11]). Despite the similarity in nomenclature, there is absolutely no evolutionary conservation (homology) or functional relationship between the mammalian AP-2 and the malarial ApiAP2 proteins as the authors suggest. The authors also incorrectly cite a recent in-depth review of the ApiAP2 protein family as a source for this information [12]. As an international journal with a diverse readership, it is pertinent that this misleading information is corrected so as to prevent further confusion.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"754054"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/754054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30672024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Multiple signaling pathways are involved in the interleukine-4 regulated expression of DC-SIGN in THP-1 cell line. 在THP-1细胞系中，多种信号通路参与白介素-4调控DC-SIGN的表达。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-05-17 DOI: 10.1155/2012/357060

Changzhong Jin, Lijuan Wu, Jie Li, Meixin Fang, Linfang Cheng, Nanping Wu

{"title":"Multiple signaling pathways are involved in the interleukine-4 regulated expression of DC-SIGN in THP-1 cell line.","authors":"Changzhong Jin, Lijuan Wu, Jie Li, Meixin Fang, Linfang Cheng, Nanping Wu","doi":"10.1155/2012/357060","DOIUrl":"https://doi.org/10.1155/2012/357060","url":null,"abstract":"Dendritic cell-specific intercellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN) is an important pattern recognition receptor on dendritic cells (DCs), and its expression shows significant cytological and histological specificity, being interleukine-4 (IL-4) dependent. The signaling pathways through which IL-4 regulates expression of DC-SIGN are still unclear. We used phorbol 12-myristate 13-acetate- (PMA-) differentiated THP-1 cells as the in vitro model of monocyte/macrophage cells to study the signaling pathways involved in IL-4-regulated expression of DC-SIGN. We found that a high expression of DC-SIGN could be induced by IL-4 at the levels of mRNA and cell surface protein. Upregulated expression of DC-SIGN was almost completely blocked by the specific inhibitor of ERK pathway, and partly reduced by the specific inhibitors of JAK-STAT and NF-κB pathways. The activation of the three signaling pathways was directly confirmed by testing the phosphorylation of protein kinase within the cytoplasm and nucleus over time. The analysis of cis-acting elements of DC-SIGN promoter showed that the activity of DC-SIGN promoter without Ets-1 transcription factors binding site almost completely disappeared. Our results demonstrated that multiple signaling pathways are involved in IL-4 induced high expression of DC-SIGN on THP-1 cells, in which ERK pathway is the main signaling pathway and mediated by the Ets-1 transcription factors binding site.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"357060"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/357060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30672648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Cell and molecular biology underpinning the effects of PEDF on cancers in general and osteosarcoma in particular. 细胞和分子生物学支持PEDF对癌症的影响，特别是骨肉瘤。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-05-28 DOI: 10.1155/2012/740295

Vijay Chandolu, Crispin R Dass

引用次数: 23

Generation of biotechnology-derived Flavobacterium columnare ghosts by PhiX174 gene E-mediated inactivation and the potential as vaccine candidates against infection in grass carp. PhiX174基因e介导的生物技术衍生柱状黄杆菌幽灵的产生及其作为草鱼感染候选疫苗的潜力

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-06-10 DOI: 10.1155/2012/760730

Wenxing Zhu, Guiwen Yang, Yuyu Zhang, Jinduo Yuan, Liguo An

{"title":"Generation of biotechnology-derived Flavobacterium columnare ghosts by PhiX174 gene E-mediated inactivation and the potential as vaccine candidates against infection in grass carp.","authors":"Wenxing Zhu, Guiwen Yang, Yuyu Zhang, Jinduo Yuan, Liguo An","doi":"10.1155/2012/760730","DOIUrl":"https://doi.org/10.1155/2012/760730","url":null,"abstract":"Flavobacterium columnare is a bacterial pathogen causing high mortality rates for many freshwater fish species. Fish vaccination with a safe and effective vaccine is a potential approach for prevention and control of fish disease. Here, in order to produce bacterial ghost vaccine, a specific Flavobacterium lysis plasmid pBV-E-cat was constructed by cloning PhiX174 lysis gene E and the cat gene with the promoter of F. columnare into the prokaryotic expression vector pBV220. The plasmid was successfully electroporated into the strain F. columnare G4cpN22 after curing of its endogenous plasmid. F. columnare G4cpN22 ghosts (FCGs) were generated for the first time by gene E-mediated lysis, and the vaccine potential of FCG was investigated in grass carp (Ctenopharyngodon idellus) by intraperitoneal route. Fish immunized with FCG showed significantly higher serum agglutination titers and bactericidal activity than fish immunized with FKC or PBS. Most importantly, after challenge with the parent strain G4, the relative percent survival (RPS) of fish in FCG group (70.9%) was significantly higher than FKC group (41.9%). These results showed that FCG could confer immune protection against F. columnare infection. As a nonliving whole cell envelope preparation, FCG may provide an ideal alternative to pathogen-based vaccines against columnaris in aquaculture.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"760730"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/760730","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30706287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 37

TP53 mutation, epithelial-mesenchymal transition, and stemlike features in breast cancer subtypes. 乳腺癌亚型的TP53突变、上皮-间质转化和茎样特征

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-07-30 DOI: 10.1155/2012/254085

Danila Coradini, Marco Fornili, Federico Ambrogi, Patrizia Boracchi, Elia Biganzoli

{"title":"TP53 mutation, epithelial-mesenchymal transition, and stemlike features in breast cancer subtypes.","authors":"Danila Coradini, Marco Fornili, Federico Ambrogi, Patrizia Boracchi, Elia Biganzoli","doi":"10.1155/2012/254085","DOIUrl":"https://doi.org/10.1155/2012/254085","url":null,"abstract":"Altered p53 protein is prevalently associated with the pathologic class of triple-negative breast cancers and loss of p53 function has recently been linked to the induction of an epithelial-mesenchymal transition (EMT) and acquisition of stemness properties. We explored the association between TP53 mutational status and expression of some genes involved in the canonical TGF-β signaling pathway (the most potent EMT inducer) and in two early EMT associated events: loss of cell polarity and acquisition of stemness-associated features. We used a publicly accessible microarray dataset consisting of 251 p53-sequenced primary breast cancers. Statistical analysis indicated that mutant p53 tumors (especially those harboring a severe mutation) were consistent with the aggressive class of triple-negative cancers and that, differently from cell cultures, surgical tumors underexpressed some TGF-β related transcription factors known as involved in EMT (ID1, ID4, SMAD3, SMAD4, SMAD5, ZEB1). These unexpected findings suggest an interesting relationship between p53 mutation, mammary cell dedifferentiation, and the concomitant acquisition of stemlike properties (as indicated by the overexpression of PROM1 and NOTCH1 genes), which improve tumor cells aggressiveness as indicated by the overexpression of genes associated with cell proliferation (CDK4, CDK6, MKI67) and migration (CXCR4, MMP1).","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"254085"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/254085","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30838976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

A limited role of p53 on the ability of a Hexane fraction of American ginseng to suppress mouse colitis. p53对西洋参己烷部分抑制小鼠结肠炎能力的有限作用。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-07-30 DOI: 10.1155/2012/785739

Deepak Poudyal, Xiangli Cui, Phuong Mai Le, Tia Davis, Anne B Hofseth, Yu Jin, Alexander A Chumanevich, Michael J Wargovich, Mitzi Nagarkatti, Prakash S Nagarkatti, Anthony Windust, Lorne J Hofseth

{"title":"A limited role of p53 on the ability of a Hexane fraction of American ginseng to suppress mouse colitis.","authors":"Deepak Poudyal, Xiangli Cui, Phuong Mai Le, Tia Davis, Anne B Hofseth, Yu Jin, Alexander A Chumanevich, Michael J Wargovich, Mitzi Nagarkatti, Prakash S Nagarkatti, Anthony Windust, Lorne J Hofseth","doi":"10.1155/2012/785739","DOIUrl":"https://doi.org/10.1155/2012/785739","url":null,"abstract":"Ulcerative colitis (UC) is debilitating and carries a high colon cancer risk. Apoptosis of inflammatory cells is a key mechanism regulating UC. We have recently shown that American ginseng (AG), and to a greater extent, a Hexane fraction of AG (HAG) can cause apoptosis and suppress mouse colitis through a p53-mediated mechanism. Here, we tested the hypothesis that HAG suppresses colitis through a p53 mechanism. We found only a limited impact of p53 in the ability of HAG to induce inflammatory cell apoptosis and suppress mouse colitis in vitro and in vivo. Finally, we asked whether HAG could cause cell cycle arrest of HCT116 colon cancer cells in vitro. Interestingly, HAG caused a G1 arrest of such cells independent of p53 status. Findings are significant because HAG suppresses colitis and associated colon cancer, and mutation in p53 is observed in most colitis-driven colon cancers. Therefore, HAG might be very effective in targeting the inflammatory cells and cancer cells since it induces apoptosis of inflammatory cells and cell cycle arrest in both p53-/- and WT p53 colon cancer cells.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"785739"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/785739","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30840346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Attenuating effect of Ginkgo biloba leaves extract on liver fibrosis induced by thioacetamide in mice. 银杏叶提取物对硫乙酰胺致小鼠肝纤维化的减弱作用。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-10-02 DOI: 10.1155/2012/761450

Atef M Al-Attar

{"title":"Attenuating effect of Ginkgo biloba leaves extract on liver fibrosis induced by thioacetamide in mice.","authors":"Atef M Al-Attar","doi":"10.1155/2012/761450","DOIUrl":"https://doi.org/10.1155/2012/761450","url":null,"abstract":"The purpose of this study is to investigate the effect of Ginkgo biloba leaves extract on experimental liver fibrosis induced by thioacetamide (TAA) in male albino mice. The experimental mice were divided into four groups. The mice of the first group were served as control. The experimental animals of the second group were given 150 mg/kg body weight of TAA by intraperitoneal injection, twice weekly, for 9 weeks. The mice of the third group were exposed to TAA and supplemented with G. biloba leaves extract. The animals of the fourth group were supplemented with G. biloba leaves extract. The levels of plasma alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transferase, alkaline phosphatase, triglycerides, cholesterol, and low-density lipoprotein cholesterol were statistically increased while the levels of plasma total protein, albumin, glucose, and high-density lipoprotein cholesterol were significantly decreased. The levels of liver superoxide dismutase, glutathione, glycogen and total protein were notably declined, whereas the level of total lipid was increased in mice of the second group. Furthermore, microscopic examination of liver sections from mice treated with TAA showed an abnormal morphology characterized by nodular transformations in liver parenchyma which surrounded by fibrous septa. Administration of G. biloba leaves extract reduced extent and development of fibrous septa, liver cells change, and biochemical alterations in mice exposed to TAA. This study showed that G. biloba leaves extract has a potential activity against TAA-induced liver fibrosis and suggested that the chemical constituents of G. biloba are effective in modulation of oxidative stress induced by TAA.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"761450"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/761450","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30995259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 50

Controlling self-renewal and differentiation of stem cells via mechanical cues. 通过机械信号控制干细胞的自我更新和分化。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-10-02 DOI: 10.1155/2012/797410

Michele M Nava, Manuela T Raimondi, Riccardo Pietrabissa

引用次数: 201