Journal of Biomedicine and Biotechnology最新文献_第9页

The inhibitory effect of Prunella vulgaris L. on aldose reductase and protein glycation. 夏枯草对醛糖还原酶和蛋白糖基化的抑制作用。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-10-03 DOI: 10.1155/2012/928159

Hong Mei Li, Jin Kyu Kim, Jai Man Jang, Sang Oh Kwon, Cheng Bi Cui, Soon Sung Lim

引用次数: 16

Validation of a new animal model of vulnerable plaques by intravascular optical coherence tomography in vivo. 血管内光学相干断层扫描在体内验证一种新的易损斑块动物模型。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-10-03 DOI: 10.1155/2012/469726

Yan Fang, Sining Hu, Jingbo Hou, Lingbo Meng, Shaosong Zhang, Bo Yu

{"title":"Validation of a new animal model of vulnerable plaques by intravascular optical coherence tomography in vivo.","authors":"Yan Fang, Sining Hu, Jingbo Hou, Lingbo Meng, Shaosong Zhang, Bo Yu","doi":"10.1155/2012/469726","DOIUrl":"https://doi.org/10.1155/2012/469726","url":null,"abstract":"We aimed to establish a rabbit model of vulnerable plaques (VPs) with the morphology and component characteristics of human VPs and to evaluate the microstructural features of VPs in vivo using intravascular optical coherence tomography (OCT). Twelve rabbits underwent endothelial denudation of the carotid artery and consumed a 1% high-cholesterol diet (HCD). They were equally divided into two groups: group A (modified needle injury) and group B (balloon injury). OCT was undertaken thrice before injury as well as 1 h and 12 weeks after injury. The degree of acute artery injury after endothelial denudation was detected by OCT. Twelve weeks after injury, OCT showed that both groups generated VPs which had thin fibrous caps and a large lipid core, whereas plaques in group A had smaller lipid arcs (P < 0.0001). Histological findings demonstrated that a larger eccentricity index (EI) (P < 0.05) and greater infiltration of macrophages (P < 0.05) in group A than in group B. Qualitative and morphometric analyses of plaques showed a significant correlation between histological and OCT measurements. A combination of modified endothelial denudation and an HCD in rabbits produced more eccentric lesions similar to those seen in humans. These data suggest that OCT could be a useful tool for evaluation of the degree of injury and VPs in vivo.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"469726"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/469726","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30998860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Optimization of fermentation medium for the production of atrazine degrading strain Acinetobacter sp. DNS(32) by statistical analysis system. 统计分析系统优化阿特拉津降解菌株不动杆菌sp. DNS(32)发酵培养基。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-10-03 DOI: 10.1155/2012/623062

Ying Zhang, Yang Wang, Zhi-Gang Wang, Xi Wang, Huo-Sheng Guo, Dong-Fang Meng, Po-Keung Wong

{"title":"Optimization of fermentation medium for the production of atrazine degrading strain Acinetobacter sp. DNS(32) by statistical analysis system.","authors":"Ying Zhang, Yang Wang, Zhi-Gang Wang, Xi Wang, Huo-Sheng Guo, Dong-Fang Meng, Po-Keung Wong","doi":"10.1155/2012/623062","DOIUrl":"https://doi.org/10.1155/2012/623062","url":null,"abstract":"Statistical experimental designs provided by statistical analysis system (SAS) software were applied to optimize the fermentation medium composition for the production of atrazine-degrading Acinetobacter sp. DNS(32) in shake-flask cultures. A \"Plackett-Burman Design\" was employed to evaluate the effects of different components in the medium. The concentrations of corn flour, soybean flour, and K(2)HPO(4) were found to significantly influence Acinetobacter sp. DNS(32) production. The steepest ascent method was employed to determine the optimal regions of these three significant factors. Then, these three factors were optimized using central composite design of \"response surface methodology.\" The optimized fermentation medium composition was composed as follows (g/L): corn flour 39.49, soybean flour 25.64, CaCO(3) 3, K(2)HPO(4) 3.27, MgSO(4)·7H(2)O 0.2, and NaCl 0.2. The predicted and verifiable values in the medium with optimized concentration of components in shake flasks experiments were 7.079 × 10(8) CFU/mL and 7.194 × 10(8) CFU/mL, respectively. The validated model can precisely predict the growth of atrazine-degraing bacterium, Acinetobacter sp. DNS(32).","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"623062"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/623062","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30998864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Bone marrow microenvironment in multiple myeloma progression. 多发性骨髓瘤进展中的骨髓微环境。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-10-03 DOI: 10.1155/2012/157496

S Manier, A Sacco, X Leleu, I M Ghobrial, A M Roccaro

引用次数: 270

Induction of apoptosis and cell cycle arrest in human colorectal carcinoma by Litchi seed extract. 荔枝籽提取物诱导人结直肠癌细胞凋亡和细胞周期阻滞。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-10-03 DOI: 10.1155/2012/341479

Chih-Ping Hsu, Chih-Cheng Lin, Chiu-Chen Huang, Yi-Hsien Lin, Jyh-Ching Chou, Yu-Ting Tsia, Jhih-Rou Su, Yuan-Chiang Chung

引用次数: 36

Production of adenosine by ectonucleotidases: a key factor in tumor immunoescape. 胞外核苷酸酶产生腺苷:肿瘤免疫逃逸的关键因素。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-10-14 DOI: 10.1155/2012/473712

François Ghiringhelli, Mélanie Bruchard, Fanny Chalmin, Cédric Rébé

引用次数: 101

What risk assessments of genetically modified organisms can learn from institutional analyses of public health risks. 转基因生物的风险评估可以从公共健康风险的机构分析中学到什么?

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-11-04 DOI: 10.1155/2012/203093

S Ravi Rajan, Deborah K Letourneau

{"title":"What risk assessments of genetically modified organisms can learn from institutional analyses of public health risks.","authors":"S Ravi Rajan, Deborah K Letourneau","doi":"10.1155/2012/203093","DOIUrl":"https://doi.org/10.1155/2012/203093","url":null,"abstract":"The risks of genetically modified organisms (GMOs) are evaluated traditionally by combining hazard identification and exposure estimates to provide decision support for regulatory agencies. We question the utility of the classical risk paradigm and discuss its evolution in GMO risk assessment. First, we consider the problem of uncertainty, by comparing risk assessment for environmental toxins in the public health domain with genetically modified organisms in the environment; we use the specific comparison of an insecticide to a transgenic, insecticidal food crop. Next, we examine normal accident theory (NAT) as a heuristic to consider runaway effects of GMOs, such as negative community level consequences of gene flow from transgenic, insecticidal crops. These examples illustrate how risk assessments are made more complex and contentious by both their inherent uncertainty and the inevitability of failure beyond expectation in complex systems. We emphasize the value of conducting decision-support research, embracing uncertainty, increasing transparency, and building interdisciplinary institutions that can address the complex interactions between ecosystems and society. In particular, we argue against black boxing risk analysis, and for a program to educate policy makers about uncertainty and complexity, so that eventually, decision making is not the burden that falls upon scientists but is assumed by the public at large.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"203093"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/203093","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31083690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

The role of TLR and chemokine in wear particle-induced aseptic loosening. TLR和趋化因子在磨损颗粒诱导的无菌性松动中的作用。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-10-21 DOI: 10.1155/2012/596870

Qiaoli Gu, Qin Shi, Huilin Yang

{"title":"The role of TLR and chemokine in wear particle-induced aseptic loosening.","authors":"Qiaoli Gu, Qin Shi, Huilin Yang","doi":"10.1155/2012/596870","DOIUrl":"https://doi.org/10.1155/2012/596870","url":null,"abstract":"Wear particle-induced periprosthetic osteolysis remains the principal cause of aseptic loosening of orthopaedic implants. Monocytes/macrophages phagocytose wear particles and release cytokines that induce inflammatory response. This response promotes osteoclast differentiation and osteolysis. The precise mechanisms by which wear particles are recognized and induce the accumulation of inflammatory cells in the periprosthetic tissue have not been fully elucidated. Recent studies have shown that toll-like receptors (TLRs) contribute to the cellular interaction with wear particles. Wear particles are recognized by monocytes/macrophages through TLRs coupled with the adaptor protein MyD88. After the initial interaction, wear particles induce both local and systemic migration of monocytes/macrophages to the periprosthetic region. The cellular migration is mediated through chemokines including interleukin-8, macrophage chemotactic protein-1, and macrophage inhibitory protein-1 in the periprosthetic tissues. Interfering with chemokine-receptor axis can inhibit cellular migration and inflammatory response. This paper highlights recent advances in TLR, and chemokine participated in the pathogenesis of aseptic loosening. A comprehensive understanding of the recognition and migration mechanism is critical to the development of measures that prevent wear particle-induced aseptic loosening of orthopaedic implants.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"596870"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/596870","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31084155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 42

Ultrastructural evidence of exosome secretion by progenitor cells in adult mouse myocardium and adult human cardiospheres. 成年小鼠心肌和成人心球祖细胞分泌外泌体的超微结构证据。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-11-20 DOI: 10.1155/2012/354605

Lucio Barile, Mihaela Gherghiceanu, Laurentiu M Popescu, Tiziano Moccetti, Giuseppe Vassalli

{"title":"Ultrastructural evidence of exosome secretion by progenitor cells in adult mouse myocardium and adult human cardiospheres.","authors":"Lucio Barile, Mihaela Gherghiceanu, Laurentiu M Popescu, Tiziano Moccetti, Giuseppe Vassalli","doi":"10.1155/2012/354605","DOIUrl":"https://doi.org/10.1155/2012/354605","url":null,"abstract":"The demonstration of beneficial effects of cell therapy despite the persistence of only few transplanted cells in vivo suggests secreted factors may be the active component of this treatment. This so-called paracrine hypothesis is supported by observations that culture media conditioned by progenitor cells contain growth factors that mediate proangiogenic and cytoprotective effects. Cardiac progenitor cells in semi-suspension culture form spherical clusters (cardiospheres) that deliver paracrine signals to neighboring cells. A key component of paracrine secretion is exosomes, membrane vesicles that are stored intracellularly in endosomal compartments and are secreted when these structures fuse with the cell plasma membrane. Exosomes have been identified as the active component of proangiogenic effects of bone marrow CD34⁺ stem cells in mice and the regenerative effects of embryonic mesenchymal stem cells in infarcted hearts in pigs and mice. Here, we provide electron microscopic evidence of exosome secretion by progenitor cells in mouse myocardium and human cardiospheres. Exosomes are emerging as an attractive vector of paracrine signals delivered by progenitor cells. They can be stored as an \"off-the-shelf\" product. As such, exosomes have the potential for circumventing many of the limitations of viable cells for therapeutic applications in regenerative medicine.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"354605"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/354605","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31111418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 74

Improved binding activity of antibodies against major histocompatibility complex class I chain-related gene A by phage display technology for cancer-targeted therapy. 利用噬菌体展示技术提高抗主要组织相容性复合体I类链相关基因A抗体的结合活性，用于癌症靶向治疗。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-11-21 DOI: 10.1155/2012/597647

Achara Phumyen, Amonrat Jumnainsong, Chanvit Leelayuwat

{"title":"Improved binding activity of antibodies against major histocompatibility complex class I chain-related gene A by phage display technology for cancer-targeted therapy.","authors":"Achara Phumyen, Amonrat Jumnainsong, Chanvit Leelayuwat","doi":"10.1155/2012/597647","DOIUrl":"https://doi.org/10.1155/2012/597647","url":null,"abstract":"Major histocompatibility complex class I chain-related gene A (MICA) is an NKG2D ligand that is over-expressed under cellular stress including cancer transformation and viral infection. High expression of MICA in cancer tissues or patients' sera is useful for prognostic or follow-up markers in cancer patients. In this study, phage display technology was employed to improve antigen-binding activities of anti-MICA monoclonal antibodies (WW2G8, WW6B7, and WW9B8). The 12 amino acid residues in the complementarity determining regions (CDRs) on the V domain of the heavy chain CDR3 (HCDR3) of these anti-MICA antibodies were modified by PCR-random mutagenesis, and phages displaying mutated anti-MICA Fab were constructed. After seven rounds of panning, five clones of phages displaying mutant anti-MICA Fab which exhibited 3-7-folds higher antigen-binding activities were isolated. Two clones of the mutants (phage-displayed mutant Fab WW9B8.1 and phage-displayed mutant Fab WW9B8.21) were confirmed to have antigen-binding specificity for cell surface MICA proteins by flow cytometry. These phage clones are able to recognize MICA in a native form according to positive results obtained by indirect ELISA and flow cytometry. Thus, these phage particles could be potentially used for further development of nanomedicine specifically targeting cancer cells expressing MICA proteins.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"597647"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/597647","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31112942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5