Journal of Biomedicine and Biotechnology最新文献_第10页

In silico biology of H1N1: molecular modelling of novel receptors and docking studies of inhibitors to reveal new insight in flu treatment. H1N1的硅生物学:新型受体的分子建模和抑制剂的对接研究揭示了流感治疗的新见解。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 DOI: 10.1155/2012/714623

Deepak Kumar Behera, Pabitra Mohan Behera, Laxmikanta Acharya, Anshuman Dixit, Payodhar Padhi

{"title":"In silico biology of H1N1: molecular modelling of novel receptors and docking studies of inhibitors to reveal new insight in flu treatment.","authors":"Deepak Kumar Behera, Pabitra Mohan Behera, Laxmikanta Acharya, Anshuman Dixit, Payodhar Padhi","doi":"10.1155/2012/714623","DOIUrl":"https://doi.org/10.1155/2012/714623","url":null,"abstract":"Influenza is an infectious disease caused by RNA viruses of the family Orthomyxoviridae. The new influenza H1N1 viral stain has emerged by the genetic combination of genes from human, pig, and bird's H1N1 virus. The influenza virus is roughly spherical and is enveloped by a lipid membrane. There are two glycoproteins in this lipid membrane; namely, hemagglutinin (HA) which helps in attachment of the viral strain on the host cell surface and neuraminidase (NA) that is responsible for initiation of viral infection. We have developed homology models of both Hemagglutinin and Neuraminidase receptors from H1N1 strains in eastern India. The docking studies of B-Sialic acid and O-Sialic acid in the optimized and energy-minimized homology models show important H-bonding interactions with ALA142, ASP230, GLN231, GLU232, and THR141. This information can be used for structure-based and pharmacophore-based new drug design. We have also calculated ADME properties (Human Oral Absorption (HOA) and % HOA) for Oseltamivir which have been subject of debate for long.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":"2012 ","pages":"714623"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/714623","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9368135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Three-dimensional visualization with large data sets: a simulation of spreading cortical depression in human brain. 具有大数据集的三维可视化:人类大脑皮层扩展凹陷的模拟。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 DOI: 10.1155/2012/352795

Korhan Levent Ertürk, Gökhan Şengül

引用次数: 3

Vaxjo: a web-based vaccine adjuvant database and its application for analysis of vaccine adjuvants and their uses in vaccine development. Vaxjo:基于网络的疫苗佐剂数据库及其用于分析疫苗佐剂及其在疫苗开发中的用途的应用。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 DOI: 10.1155/2012/831486

Samantha Sayers, Guerlain Ulysse, Zuoshuang Xiang, Yongqun He

{"title":"Vaxjo: a web-based vaccine adjuvant database and its application for analysis of vaccine adjuvants and their uses in vaccine development.","authors":"Samantha Sayers, Guerlain Ulysse, Zuoshuang Xiang, Yongqun He","doi":"10.1155/2012/831486","DOIUrl":"https://doi.org/10.1155/2012/831486","url":null,"abstract":"Vaccine adjuvants are compounds that enhance host immune responses to co-administered antigens in vaccines. Vaxjo is a web-based central database and analysis system that curates, stores, and analyzes vaccine adjuvants and their usages in vaccine development. Basic information of a vaccine adjuvant stored in Vaxjo includes adjuvant name, components, structure, appearance, storage, preparation, function, safety, and vaccines that use this adjuvant. Reliable references are curated and cited. Bioinformatics scripts are developed and used to link vaccine adjuvants to different adjuvanted vaccines stored in the general VIOLIN vaccine database. Presently, 103 vaccine adjuvants have been curated in Vaxjo. Among these adjuvants, 98 have been used in 384 vaccines stored in VIOLIN against over 81 pathogens, cancers, or allergies. All these vaccine adjuvants are categorized and analyzed based on adjuvant types, pathogens used, and vaccine types. As a use case study of vaccine adjuvants in infectious disease vaccines, the adjuvants used in Brucella vaccines are specifically analyzed. A user-friendly web query and visualization interface is developed for interactive vaccine adjuvant search. To support data exchange, the information of vaccine adjuvants is stored in the Vaccine Ontology (VO) in the Web Ontology Language (OWL) format.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":"2012 ","pages":"831486"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/831486","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9374943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 50

Bacterial plasminogen receptors utilize host plasminogen system for effective invasion and dissemination. 细菌的纤溶酶原受体利用宿主的纤溶酶原系统进行有效的入侵和传播。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 DOI: 10.1155/2012/482096

Sarbani Bhattacharya, Victoria A Ploplis, Francis J Castellino

{"title":"Bacterial plasminogen receptors utilize host plasminogen system for effective invasion and dissemination.","authors":"Sarbani Bhattacharya, Victoria A Ploplis, Francis J Castellino","doi":"10.1155/2012/482096","DOIUrl":"https://doi.org/10.1155/2012/482096","url":null,"abstract":"In order for invasive pathogens to migrate beyond the site of infection, host physiological barriers such as the extracellular matrix, the basement membrane, and encapsulating fibrin network must be degraded. To circumvent these impediments, proteolytic enzymes facilitate the dissemination of the microorganism. Recruitment of host proteases to the bacterial surface represents a particularly effective mechanism for enhancing invasiveness. Plasmin is a broad spectrum serine protease that degrades fibrin, extracellular matrices, and connective tissue. A large number of pathogens express plasminogen receptors which immobilize plasmin(ogen) on the bacterial surface. Surface-bound plasminogen is then activated by plasminogen activators to plasmin through limited proteolysis thus triggering the development of a proteolytic surface on the bacteria and eventually assisting the spread of bacteria. The host hemostatic system plays an important role in systemic infection. The interplay between hemostatic processes such as coagulation and fibrinolysis and the inflammatory response constitutes essential components of host defense and bacterial invasion. The goal of this paper is to highlight mechanisms whereby pathogenic bacteria, by engaging surface receptors, utilize and exploit the host plasminogen and fibrinolytic system for the successful dissemination within the host.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":"2012 ","pages":"482096"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/482096","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9375597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 125

Five hundred years of mercury exposure and adaptation. 五百年的汞暴露和适应。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 DOI: 10.1155/2012/472858

Guido Lombardi, Antonio Lanzirotti, Clifford Qualls, Francisco Socola, Abdul-Mehdi Ali, Otto Appenzeller

{"title":"Five hundred years of mercury exposure and adaptation.","authors":"Guido Lombardi, Antonio Lanzirotti, Clifford Qualls, Francisco Socola, Abdul-Mehdi Ali, Otto Appenzeller","doi":"10.1155/2012/472858","DOIUrl":"https://doi.org/10.1155/2012/472858","url":null,"abstract":"Mercury is added to the biosphere by anthropogenic activities raising the question of whether changes in the human chromatin, induced by mercury, in a parental generation could allow adaptation of their descendants to mercury. We review the history of Andean mining since pre-Hispanic times in Huancavelica, Peru. Despite the persistent degradation of the biosphere today, no overt signs of mercury toxicity could be discerned in present day inhabitants. However, mercury is especially toxic to the autonomic nervous system (ANS). We, therefore, tested ANS function and biologic rhythms, under the control of the ANS, in 5 Huancavelicans and examined the metal content in their hair. Mercury levels varied from none to 1.014 ppm, significantly less than accepted standards. This was confirmed by microfocused synchrotron X-ray fluorescence analysis. Biologic rhythms were abnormal and hair growth rate per year, also under ANS control, was reduced (P < 0.001). Thus, evidence of mercury's toxicity in ANS function was found without other signs of intoxication. Our findings are consistent with the hypothesis of partial transgenerational inheritance of tolerance to mercury in Huancavelica, Peru. This would generally benefit survival in the Anthropocene, the man-made world, we now live in.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":"2012 ","pages":"472858"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/472858","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9434554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Approaches for the development of rapid serological assays for surveillance and diagnosis of infections caused by zoonotic flaviviruses of the Japanese encephalitis virus serocomplex. 开发用于监测和诊断由日本脑炎病毒血清复合体中的人畜共患黄病毒引起的感染的快速血清学测定方法。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-04-18 DOI: 10.1155/2012/379738

Jody Hobson-Peters

引用次数: 0

Nanoparticulate adjuvants and delivery systems for allergen immunotherapy. 用于过敏原免疫疗法的纳米微粒佐剂和输送系统。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-02-26 DOI: 10.1155/2012/474605

Juliana De Souza Rebouças, Irene Esparza, Marta Ferrer, María Luisa Sanz, Juan Manuel Irache, Carlos Gamazo

{"title":"Nanoparticulate adjuvants and delivery systems for allergen immunotherapy.","authors":"Juliana De Souza Rebouças, Irene Esparza, Marta Ferrer, María Luisa Sanz, Juan Manuel Irache, Carlos Gamazo","doi":"10.1155/2012/474605","DOIUrl":"10.1155/2012/474605","url":null,"abstract":"In the last decades, significant progress in research and clinics has been made to offer possible innovative therapeutics for the management of allergic diseases. However, current allergen immunotherapy shows limitations concerning the long-term efficacy and safety due to local side effects and risk of anaphylaxis. Thus, effective and safe vaccines with reduced dose of allergen have been developed using adjuvants. Nevertheless, the use of adjuvants still has several disadvantages, which limits its use in human vaccines. In this context, several novel adjuvants for allergen immunotherapy are currently being investigated and developed. Currently, nanoparticles-based allergen-delivery systems have received much interest as potential adjuvants for allergen immunotherapy. It has been demonstrated that the incorporation of allergens into a delivery system plays an important role in the efficacy of allergy vaccines. Several nanoparticles-based delivery systems have been described, including biodegradable and nondegradable polymeric carriers. Therefore, this paper provides an overview of the current adjuvants used for allergen immunotherapy. Furthermore, nanoparticles-based allergen-delivery systems are focused as a novel and promising strategy for allergy vaccines.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":"2012 ","pages":"474605"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3303624/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9367589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A classification method of normal and overweight females based on facial features for automated medical applications. 基于面部特征的正常和超重女性分类方法，用于自动医疗应用。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-08-05 DOI: 10.1155/2012/834578

Bum Ju Lee, Jun-Hyeong Do, Jong Yeol Kim

{"title":"A classification method of normal and overweight females based on facial features for automated medical applications.","authors":"Bum Ju Lee, Jun-Hyeong Do, Jong Yeol Kim","doi":"10.1155/2012/834578","DOIUrl":"10.1155/2012/834578","url":null,"abstract":"Obesity and overweight have become serious public health problems worldwide. Obesity and abdominal obesity are associated with type 2 diabetes, cardiovascular diseases, and metabolic syndrome. In this paper, we first suggest a method of predicting normal and overweight females according to body mass index (BMI) based on facial features. A total of 688 subjects participated in this study. We obtained the area under the ROC curve (AUC) value of 0.861 and kappa value of 0.521 in Female: 21-40 (females aged 21-40 years) group, and AUC value of 0.76 and kappa value of 0.401 in Female: 41-60 (females aged 41-60 years) group. In two groups, we found many features showing statistical differences between normal and overweight subjects by using an independent two-sample t-test. We demonstrated that it is possible to predict BMI status using facial characteristics. Our results provide useful information for studies of obesity and facial characteristics, and may provide useful clues in the development of applications for alternative diagnosis of obesity in remote healthcare.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":"2012 ","pages":"834578"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3420233/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9375473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficiency of ferritin as an MRI reporter gene in NPC cells is enhanced by iron supplementation. 铁蛋白作为鼻咽癌细胞的MRI报告基因的效率通过补充铁而增强。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-03-20 DOI: 10.1155/2012/434878

Yupeng Feng, Qicai Liu, Junfeng Zhu, Fukang Xie, Li Li

{"title":"Efficiency of ferritin as an MRI reporter gene in NPC cells is enhanced by iron supplementation.","authors":"Yupeng Feng, Qicai Liu, Junfeng Zhu, Fukang Xie, Li Li","doi":"10.1155/2012/434878","DOIUrl":"https://doi.org/10.1155/2012/434878","url":null,"abstract":"Background: An emerging MRI reporter, ferritin heavy chain (FTH1), is recently applied to enhance the contrast and increase the sensitivity of MRI in the monitoring of solid tumors. However, FTH1-overexpression-related cytotoxicity is required to be explored.Methods: By using the Tet-Off system, FTH1 overexpression was semi-quantitativiely and dynamicly regulated by doxycycline in a NPC cell line. Effects of FTH1 overexpression on the proliferation, cytotoxicity, apoptosis and migration of NPC cells were investigated in vitro, and MR relaxation rate was measured in vitro and in vivo.Results: In vitro and in vivo overexpression of FTH1 significantly increased the transverse relaxivity (R(2)), which could be enhanced by iron supplementation. In vitro, overexpression of FTH1 reduced cell growth and migration, which were not reduced by iron supplementation. Furthermore, cells were subcutaneously inoculated into the nude mice. Results showed FTH1 overexpression decreased tumor growth in the absence of iron supplementation but not in the presence of iron supplementation.Conclusion: To maximize R(2) and minimize the potential adverse effects, supplementation of iron at appropriate dose is recommended during the application of FTH1 as a reporter gene in the monitoring of NPC by MRI.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"434878"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/434878","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40184145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Self-organization of motor-propelled cytoskeletal filaments at topographically defined borders. 在地形上确定的边界上运动推进的细胞骨架细丝的自组织。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-03-26 DOI: 10.1155/2012/647265

Alf Månsson, Richard Bunk, Mark Sundberg, Lars Montelius

{"title":"Self-organization of motor-propelled cytoskeletal filaments at topographically defined borders.","authors":"Alf Månsson, Richard Bunk, Mark Sundberg, Lars Montelius","doi":"10.1155/2012/647265","DOIUrl":"https://doi.org/10.1155/2012/647265","url":null,"abstract":"Self-organization phenomena are of critical importance in living organisms and of great interest to exploit in nanotechnology. Here we describe in vitro self-organization of molecular motor-propelled actin filaments, manifested as a tendency of the filaments to accumulate in high density close to topographically defined edges on nano- and microstructured surfaces. We hypothesized that this \"edge-tracing\" effect either (1) results from increased motor density along the guiding edges or (2) is a direct consequence of the asymmetric constraints on stochastic changes in filament sliding direction imposed by the edges. The latter hypothesis is well captured by a model explicitly defining the constraints of motility on structured surfaces in combination with Monte-Carlo simulations [cf. Nitta et al. (2006)] of filament sliding. In support of hypothesis 2 we found that the model reproduced the edge tracing effect without the need to assume increased motor density at the edges. We then used model simulations to elucidate mechanistic details. The results are discussed in relation to nanotechnological applications and future experiments to test model predictions.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"647265"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/647265","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40184063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20