Journal of Biomedicine and Biotechnology最新文献_第6页

Antilisterial activity of nisin-like bacteriocin-producing Lactococcus lactis subsp. lactis isolated from traditional Sardinian dairy products. 产乳酸菌素样细菌素乳酸乳球菌亚种的抗李斯特菌活性。从传统撒丁岛乳制品中分离出来的乳酸菌。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-03-27 DOI: 10.1155/2012/376428

Sofia Cosentino, Maria Elisabetta Fadda, Maura Deplano, Roberta Melis, Rita Pomata, Maria Barbara Pisano

{"title":"Antilisterial activity of nisin-like bacteriocin-producing Lactococcus lactis subsp. lactis isolated from traditional Sardinian dairy products.","authors":"Sofia Cosentino, Maria Elisabetta Fadda, Maura Deplano, Roberta Melis, Rita Pomata, Maria Barbara Pisano","doi":"10.1155/2012/376428","DOIUrl":"https://doi.org/10.1155/2012/376428","url":null,"abstract":"With the aim of selecting LAB strains with antilisterial activity to be used as protective cultures to enhance the safety of dairy products, the antimicrobial properties of 117 Lactococcus lactis subsp. lactis isolated from artisanal Sardinian dairy products were evaluated, and six strains were found to produce bacteriocin-like substances. The capacity of these strains to antagonize Listeria monocytogenes during cocultivation in skimmed milk was evaluated, showing a reduction of L. monocytogenes counts of approximately 4 log units compared to the positive control after 24 h of incubation. In order for a strain to be used as bioprotective culture, it should be carefully evaluated for the presence of virulence factors, to determine what potential risks might be involved in its use. None of the strains tested was found to produce biogenic amines or to possess haemolytic activity. In addition, all strains were sensitive to clinically important antibiotics such as ampicillin, tetracycline, and vancomycin. Our results suggest that these bac+ strains could be potentially applied in cheese manufacturing to control the growth of L. monocytogenes.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"376428"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/376428","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40184142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 45

Advances in arbovirus surveillance, detection and diagnosis. 虫媒病毒监测、检测和诊断的进展。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-05-16 DOI: 10.1155/2012/512969

Roy A Hall, Bradley J Blitvich, Cheryl A Johansen, Stuart D Blacksell

引用次数: 23

Bacterial artificial chromosome clones of viruses comprising the towne cytomegalovirus vaccine. 含有巨细胞病毒疫苗的细菌人工染色体克隆病毒。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2011-11-30 DOI: 10.1155/2012/428498

Xiaohong Cui, Stuart P Adler, Andrew J Davison, Larry Smith, El-Sayed E Habib, Michael A McVoy

引用次数: 0

The effect of Tinospora crispa on serum glucose and insulin levels in patients with type 2 diabetes mellitus. crispa对2型糖尿病患者血糖和胰岛素水平的影响。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2011-11-02 DOI: 10.1155/2012/808762

Theerawut Klangjareonchai, Chulaporn Roongpisuthipong

{"title":"The effect of Tinospora crispa on serum glucose and insulin levels in patients with type 2 diabetes mellitus.","authors":"Theerawut Klangjareonchai, Chulaporn Roongpisuthipong","doi":"10.1155/2012/808762","DOIUrl":"https://doi.org/10.1155/2012/808762","url":null,"abstract":"Objective: To determine the effects of Tinospora crispa on serum glucose and insulin levels in healthy subjects and patients with type 2 diabetes mellitus.Method: Serum from 10 healthy subjects and 10 diabetic participants, who had fasted overnight, were obtained every 30-60 minutes during the 3 hours of continued fasting and during the 3 hours after ingestion of 75 g of glucose with or without ingestion of 125 or 250 g of Tinospora crispa dry powder capsule. Glucose and Insulin levels were analyzed and the areas under the curve for mean serum glucose and insulin levels were calculated.Result: The areas under the curve of mean serum glucose and insulin levels in both healthy and diabetic participants were not significantly different between with or without Tinospora crispa dry powder capsule. In diabetic participants the area under the curve of glucose was slightly lesser when 250 mg of Tinospora crispa was ingested, but not reaching statistical significance (478 and 444 mg min/ml, resp., P = 0.57).Conclusion: The results suggest that Tinospora crispa ingestion cannot affect serum glucose and insulin levels in healthy subjects or patients with type 2 diabetes mellitus.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"808762"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/808762","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30293242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Urinary neutrophil gelatinase-associated lipocalin is a potential biomarker for renal damage in patients with systemic lupus erythematosus. 尿中性粒细胞明胶酶相关脂钙蛋白是系统性红斑狼疮患者肾脏损害的潜在生物标志物。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-02-15 DOI: 10.1155/2012/759313

Chun-Chen Yang, Song-Chou Hsieh, Ko-Jen Li, Cheng-Han Wu, Ming-Chi Lu, Chang-Youh Tsai, Chia-Li Yu

{"title":"Urinary neutrophil gelatinase-associated lipocalin is a potential biomarker for renal damage in patients with systemic lupus erythematosus.","authors":"Chun-Chen Yang, Song-Chou Hsieh, Ko-Jen Li, Cheng-Han Wu, Ming-Chi Lu, Chang-Youh Tsai, Chia-Li Yu","doi":"10.1155/2012/759313","DOIUrl":"https://doi.org/10.1155/2012/759313","url":null,"abstract":"Neutrophil gelatinase-associated lipocalin (NGAL) has been demonstrated to be a novel biomarker in acute and chronic kidney disease. We hypothesized that 24-hour urinary NGAL excretion may be a predictor for renal damage in patients with systemic lupus erythematosus (SLE). Thirty-four SLE patients with renal involvement (SLE-renal group), 8 SLE patients without renal involvement (SLE-nonrenal group), 14 patients with non-SLE autoimmune diseases (disease control or DC group), and 12 healthy volunteers (normal control or NC group) were compared for 24-hour urinary excretion of NGAL and different cytokines. We found that the 24-hour urinary NGAL excretion in the SLE-renal group was higher than that in the SLE-non-renal, DC, and NC groups. However, the excretion of interleukin-10, transforming growth factor-β1, and tumor necrosis factor-α was not different between the SLE-renal and SLE-non-renal groups. Furthermore, NGAL excretion in the SLE-renal group was correlated with serum creatinine levels and creatinine clearance, but not with the SLE Disease Activity Index score. Multivariate logistic regression analysis and receiver operating characteristic curve analysis revealed that 24-hour urinary NGAL excretion is a potential biomarker for renal damage in SLE patients, with higher sensitivity and specificity than anti-dsDNA antibody titers.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"759313"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/759313","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30573014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 40

Molecular modeling of the M3 acetylcholine muscarinic receptor and its binding site. M3乙酰胆碱毒蕈碱受体及其结合位点的分子模拟。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-02-27 DOI: 10.1155/2012/789741

Marlet Martinez-Archundia, Arnau Cordomi, Pere Garriga, Juan J Perez

{"title":"Molecular modeling of the M3 acetylcholine muscarinic receptor and its binding site.","authors":"Marlet Martinez-Archundia, Arnau Cordomi, Pere Garriga, Juan J Perez","doi":"10.1155/2012/789741","DOIUrl":"https://doi.org/10.1155/2012/789741","url":null,"abstract":"The present study reports the results of a combined computational and site mutagenesis study designed to provide new insights into the orthosteric binding site of the human M3 muscarinic acetylcholine receptor. For this purpose a three-dimensional structure of the receptor at atomic resolution was built by homology modeling, using the crystallographic structure of bovine rhodopsin as a template. Then, the antagonist N-methylscopolamine was docked in the model and subsequently embedded in a lipid bilayer for its refinement using molecular dynamics simulations. Two different lipid bilayer compositions were studied: one component palmitoyl-oleyl phosphatidylcholine (POPC) and two-component palmitoyl-oleyl phosphatidylcholine/palmitoyl-oleyl phosphatidylserine (POPC-POPS). Analysis of the results suggested that residues F222 and T235 may contribute to the ligand-receptor recognition. Accordingly, alanine mutants at positions 222 and 235 were constructed, expressed, and their binding properties determined. The results confirmed the role of these residues in modulating the binding affinity of the ligand.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"789741"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/789741","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30573491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Using medical history embedded in biometrics medical card for user identity authentication: privacy preserving authentication model by features matching. 利用嵌入生物特征医疗卡的病历进行用户身份认证:基于特征匹配的隐私保护认证模型。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-04-05 DOI: 10.1155/2012/403987

Simon Fong, Yan Zhuang

{"title":"Using medical history embedded in biometrics medical card for user identity authentication: privacy preserving authentication model by features matching.","authors":"Simon Fong, Yan Zhuang","doi":"10.1155/2012/403987","DOIUrl":"https://doi.org/10.1155/2012/403987","url":null,"abstract":"Many forms of biometrics have been proposed and studied for biometrics authentication. Recently researchers are looking into longitudinal pattern matching that based on more than just a singular biometrics; data from user's activities are used to characterise the identity of a user. In this paper we advocate a novel type of authentication by using a user's medical history which can be electronically stored in a biometric security card. This is a sequel paper from our previous work about defining abstract format of medical data to be queried and tested upon authentication. The challenge to overcome is preserving the user's privacy by choosing only the useful features from the medical data for use in authentication. The features should contain less sensitive elements and they are implicitly related to the target illness. Therefore exchanging questions and answers about a few carefully chosen features in an open channel would not easily or directly expose the illness, but yet it can verify by inference whether the user has a record of it stored in his smart card. The design of a privacy preserving model by backward inference is introduced in this paper. Some live medical data are used in experiments for validation and demonstration.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"403987"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/403987","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30587435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Usefulness of FDG, MET and FLT-PET studies for the management of human gliomas. FDG, MET和FLT-PET研究在人脑胶质瘤治疗中的应用。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-04-11 DOI: 10.1155/2012/205818

Keisuke Miyake, Aya Shinomiya, Masaki Okada, Tetsuhiro Hatakeyama, Nobuyuki Kawai, Takashi Tamiya

{"title":"Usefulness of FDG, MET and FLT-PET studies for the management of human gliomas.","authors":"Keisuke Miyake, Aya Shinomiya, Masaki Okada, Tetsuhiro Hatakeyama, Nobuyuki Kawai, Takashi Tamiya","doi":"10.1155/2012/205818","DOIUrl":"https://doi.org/10.1155/2012/205818","url":null,"abstract":"The use of positron imaging agents such as FDG, MET, and FLT is expected to lead the way for novel applications toward efficient malignancy grading and treatment of gliomas. In this study, the usefulness of FDG, MET and FLT-PET images was retrospectively reviewed by comparing their histopathological findings. FDG, MET, and FLT-PET were performed in 27 patients with WHO grade IV, 15 patients with WHO grade III, and 12 patients with WHO grade II during 5.5 years. The resulting PET images were compared by measuring SUVs and T/N ratios (tumor to normal tissue ratios). Although there were no significant differences in FDG-PET, there were significant differences in the T/N ratios in the MET-PET between WHO grades II and IV and in the FLT-PET between the WHO grades III and IV. In glioblastoma patients, the SUVs of the areas depicted by MRI in the MET-PET were different from those SUVs in the FLT-PET. Importantly, the areas with high SUVs in both MET-PET and FLT-PET were also high in Ki-67 index and were histologically highly malignant. PET imaging is a noninvasive modality that is useful in determining a tumor area for removal as well as improving preoperative diagnosis for gliomas.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"205818"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/205818","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30609868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 45

Camel milk triggers apoptotic signaling pathways in human hepatoma HepG2 and breast cancer MCF7 cell lines through transcriptional mechanism. 驼奶通过转录机制触发人肝癌HepG2和乳腺癌MCF7细胞系凋亡信号通路。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-05-13 DOI: 10.1155/2012/593195

Hesham M Korashy, Zaid H Maayah, Adel R Abd-Allah, Ayman O S El-Kadi, Abdulqader A Alhaider

{"title":"Camel milk triggers apoptotic signaling pathways in human hepatoma HepG2 and breast cancer MCF7 cell lines through transcriptional mechanism.","authors":"Hesham M Korashy, Zaid H Maayah, Adel R Abd-Allah, Ayman O S El-Kadi, Abdulqader A Alhaider","doi":"10.1155/2012/593195","DOIUrl":"https://doi.org/10.1155/2012/593195","url":null,"abstract":"Few published studies have reported the use of crude camel milk in the treatment of stomach infections, tuberculosis and cancer. Yet, little research was conducted on the effect of camel milk on the apoptosis and oxidative stress associated with human cancer. The present study investigated the effect and the underlying mechanisms of camel milk on the proliferation of human cancer cells using an in vitro model of human hepatoma (HepG2) and human breast (MCF7) cancer cells. Our results showed that camel milk, but not bovine milk, significantly inhibited HepG2 and MCF7 cells proliferation through the activation of caspase-3 mRNA and activity levels, and the induction of death receptors in both cell lines. In addition, Camel milk enhanced the expression of oxidative stress markers, heme oxygenase-1 and reactive oxygen species production in both cells. Mechanistically, the increase in caspase-3 mRNA levels by camel milk was completely blocked by the transcriptional inhibitor, actinomycin D; implying that camel milk increased de novo RNA synthesis. Furthermore, Inhibition of the mitogen activated protein kinases differentially modulated the camel milk-induced caspase-3 mRNA levels. Taken together, camel milk inhibited HepG2 and MCF7 cells survival and proliferation through the activation of both the extrinsic and intrinsic apoptotic pathways.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"593195"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/593195","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30659650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 94

Cigarette smoke extract stimulates rat pulmonary artery smooth muscle cell proliferation via PKC-PDGFB signaling. 香烟提取物通过PKC-PDGFB信号通路刺激大鼠肺动脉平滑肌细胞增殖。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-06-11 DOI: 10.1155/2012/534384

Ai-ping Xing, Yong-cheng Du, Xiao-yun Hu, Jian-ying Xu, Huan-ping Zhang, Yi Li, Xin Nie

{"title":"Cigarette smoke extract stimulates rat pulmonary artery smooth muscle cell proliferation via PKC-PDGFB signaling.","authors":"Ai-ping Xing, Yong-cheng Du, Xiao-yun Hu, Jian-ying Xu, Huan-ping Zhang, Yi Li, Xin Nie","doi":"10.1155/2012/534384","DOIUrl":"https://doi.org/10.1155/2012/534384","url":null,"abstract":"Accumulating evidence suggests a direct role for cigarette smoke in pulmonary vascular remodeling, which contributes to the development of pulmonary hypertension. However, the molecular mechanisms underlying this process remain poorly understood. Platelet-derived growth factor (PDGF) is a potential mitogen and chemoattractant implicated in several biological processes, including cell survival, proliferation, and migration. In this study, we investigated the effect of cigarette smoke extract (CSE) on cell proliferation of rat pulmonary artery smooth muscle cells (rPASMCs). We found that stimulation of rPASMCs with CSE significantly increased cell proliferation and promoted cell cycle progression from G1 phase to the S and G2 phases. CSE treatment also significantly upregulated the mRNA and protein levels of PDGFB and PDGFRβ. Our study also revealed that Rottlerin, an inhibitor of PKCδ signaling, prevented CSE-induced cell proliferation, attenuated the increase of S and G2 phase populations induced by CSE treatment, and downregulated PDGFB and PDGFRβ mRNA and protein levels in rPASMCs exposed to CSE. Collectively, our data demonstrated that CSE-induced cell proliferation of rPASMCs involved upregulation of the PKCδ-PDGFB pathway.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"534384"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/534384","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30735113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18