Journal of Biomedicine and Biotechnology最新文献_第5页

Production of chemoenzymatic catalyzed monoepoxide biolubricant: optimization and physicochemical characteristics. 化学酶催化单环氧生物润滑剂的生产:优化及理化特性。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-01-19 DOI: 10.1155/2012/693848

Jumat Salimon, Nadia Salih, Bashar Mudhaffar Abdullah

{"title":"Production of chemoenzymatic catalyzed monoepoxide biolubricant: optimization and physicochemical characteristics.","authors":"Jumat Salimon, Nadia Salih, Bashar Mudhaffar Abdullah","doi":"10.1155/2012/693848","DOIUrl":"https://doi.org/10.1155/2012/693848","url":null,"abstract":"Linoleic acid (LA) is converted to per-carboxylic acid catalyzed by an immobilized lipase from Candida antarctica (Novozym 435). This per-carboxylic acid is only intermediate and epoxidized itself in good yields and almost without consecutive reactions. Monoepoxide linoleic acid 9(12)-10(13)-monoepoxy 12(9)-octadecanoic acid (MEOA) was optimized using D-optimal design. At optimum conditions, higher yield% (82.14) and medium oxirane oxygen content (OOC) (4.91%) of MEOA were predicted at 15 μL of H(2)O(2), 120 mg of Novozym 435, and 7 h of reaction time. In order to develop better-quality biolubricants, pour point (PP), flash point (FP), viscosity index (VI), and oxidative stability (OT) were determined for LA and MEOA. The results showed that MEOA exhibited good low-temperature behavior with PP of -41(°)C. FP of MEOA increased to 128(°)C comparing with 115(°)C of LA. In a similar fashion, VI for LA was 224 generally several hundred centistokes (cSt) more viscous than MEOA 130.8. The ability of a substance to resist oxidative degradation is another important property for biolubricants. Therefore, LA and MEOA were screened to measure their OT which was observed at 189 and 168(°)C, respectively.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"693848"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/693848","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30469738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Effects of IRF5 lupus risk haplotype on pathways predicted to influence B cell functions. IRF5狼疮风险单倍型对预测影响B细胞功能的途径的影响。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-02-22 DOI: 10.1155/2012/594056

Joel M Guthridge, Daniel N Clark, Amanda Templeton, Nicolas Dominguez, Rufei Lu, Gabriel S Vidal, Jennifer A Kelly, Kenneth M Kauffman, John B Harley, Patrick M Gaffney, Judith A James, Brian D Poole

{"title":"Effects of IRF5 lupus risk haplotype on pathways predicted to influence B cell functions.","authors":"Joel M Guthridge, Daniel N Clark, Amanda Templeton, Nicolas Dominguez, Rufei Lu, Gabriel S Vidal, Jennifer A Kelly, Kenneth M Kauffman, John B Harley, Patrick M Gaffney, Judith A James, Brian D Poole","doi":"10.1155/2012/594056","DOIUrl":"https://doi.org/10.1155/2012/594056","url":null,"abstract":"Both genetic and environmental interactions affect systemic lupus erythematosus (SLE) development and pathogenesis. One known genetic factor associated with lupus is a haplotype of the interferon regulatory factor 5 (IRF5) gene. Analysis of global gene expression microarray data using gene set enrichment analysis identified multiple interferon- and inflammation-related gene sets significantly overrepresented in cells with the risk haplotype. Pathway analysis using expressed genes from the significant gene sets impacted by the IRF5 risk haplotype confirmed significant correlation with the interferon pathway, Toll-like receptor pathway, and the B-cell receptor pathway. SLE patients with the IRF5 risk haplotype have a heightened interferon signature, even in an unstimulated state (P = 0.011), while patients with the IRF5 protective haplotype have a B cell interferon signature similar to that of controls. These results identify multiple genes in functionally significant pathways which are affected by IRF5 genotype. They also establish the IRF5 risk haplotype as a key determinant of not only the interferon response, but also other B-cell pathways involved in SLE.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"594056"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/594056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30573006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Sesquiterpene lactones isolated from Elephantopus scaber L. inhibits human lymphocyte proliferation and the growth of tumour cell lines and induces apoptosis in vitro. 从象皮中分离的倍半萜内酯体外抑制人淋巴细胞增殖和肿瘤细胞系生长并诱导细胞凋亡。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-02-01 DOI: 10.1155/2012/721285

B S Geetha, Mangalam S Nair, P G Latha, P Remani

{"title":"Sesquiterpene lactones isolated from Elephantopus scaber L. inhibits human lymphocyte proliferation and the growth of tumour cell lines and induces apoptosis in vitro.","authors":"B S Geetha, Mangalam S Nair, P G Latha, P Remani","doi":"10.1155/2012/721285","DOIUrl":"https://doi.org/10.1155/2012/721285","url":null,"abstract":"This study was designed to isolate the compounds responsible for the cytotoxic properties of South Indian Elephantopus scaber L. and further investigate their effects on quiescent and proliferating cells. Bioassay-guided isolation of the whole plant of chloroform extract of South Indian Elephantopus scaber afforded the known sesquiterpene lactone, deoxyelephantopin, and isodeoxyelephantopin whose structures were determined by spectroscopic methods. These compounds caused a dose dependent reduction in the viability of L-929 tumour cells in 72 h culture (IC(50) value of 2.7 μg/mL and 3.3 μg/mL) by the cell viability assay. Both the compounds act selectively on quiescent and PHA-stimulated proliferating human lymphocytes and inhibited tritiated thymidine incorporation into cellular DNA of DLA tumour cells. The compound deoxyelephantopin at a concentration of 3 μg/mL caused maximum apoptotic cells. It also exhibited significant in vivo antitumour efficacy against DLA tumour cells. The results, therefore, indicate that the antiproliferative property of deoxyelephantopin and isodeoxyelephantopin could be used in regimens for treating tumors with extensive proliferative potencies.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"721285"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/721285","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30573012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 53

Effects of saponins against clinical E. coli strains and eukaryotic cell line. 皂苷对临床大肠杆菌菌株和真核细胞系的作用。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-02-21 DOI: 10.1155/2012/286216

Michał Arabski, Aneta Węgierek-Ciuk, Grzegorz Czerwonka, Anna Lankoff, Wiesław Kaca

{"title":"Effects of saponins against clinical E. coli strains and eukaryotic cell line.","authors":"Michał Arabski, Aneta Węgierek-Ciuk, Grzegorz Czerwonka, Anna Lankoff, Wiesław Kaca","doi":"10.1155/2012/286216","DOIUrl":"https://doi.org/10.1155/2012/286216","url":null,"abstract":"Saponins are detergent-like substances showing antibacterial as well as anticancer potential. In this study, the effects of saponins from Quillaja saponaria were analyzed against prokaryotic and eukaryotic cells. Multidrug-resistant clinical E. coli strains were isolated from human urine. As eukaryotic cells, the CHO-K1 cell lines were applied. Antibacterial effect of ampicillin, streptomycin, and ciprofloxacin in the presence of saponins was measured by cultivation methods. Properties of saponins against CHO-K1 cells were measured by the MTT test, hemolysis assay and flow cytometry. Saponin from Quillaja saponaria has a cytotoxic effect at concentrations higher than 25 μg/mL and in the range of 12–50 μg/mL significantly increases the level of early apoptotic cells. Saponin at dose of 12 μg/mL enhances the six E. coli strains growth. We postulate that saponins increase the influx of nutrients from the medium into E. coli cells. Saponins do not have synergetic effects on antibacterial action of tested antibiotics. In contrary, in the presence of saponins and antibiotics, more CFU/mL E. coli cells were observed. This effect was similar to saponins action alone towards E. coli cells. In conclusion, saponins was cytotoxic against CHO-K1 cells, whereas against E. coli cells this effect was not observed.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"286216"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/286216","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30573169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 119

Studying interactions by molecular dynamics simulations at high concentration. 用分子动力学模拟研究高浓度下的相互作用。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-02-22 DOI: 10.1155/2012/303190

Federico Fogolari, Alessandra Corazza, Stefano Toppo, Silvio C E Tosatto, Paolo Viglino, Fulvio Ursini, Gennaro Esposito

引用次数: 17

In vivo testing of microRNA-mediated gene knockdown in zebrafish. 斑马鱼体内microrna介导基因敲低的实验研究。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-02-27 DOI: 10.1155/2012/350352

Ivone Un San Leong, Chuan-Ching Lan, Jonathan R Skinner, Andrew N Shelling, Donald R Love

{"title":"In vivo testing of microRNA-mediated gene knockdown in zebrafish.","authors":"Ivone Un San Leong, Chuan-Ching Lan, Jonathan R Skinner, Andrew N Shelling, Donald R Love","doi":"10.1155/2012/350352","DOIUrl":"https://doi.org/10.1155/2012/350352","url":null,"abstract":"The zebrafish (Danio rerio) has become an attractive model for human disease modeling as there are a large number of orthologous genes that encode similar proteins to those found in humans. The number of tools available to manipulate the zebrafish genome is limited and many currently used techniques are only effective during early development (such as morpholino-based antisense technology) or it is phenotypically driven and does not offer targeted gene knockdown (such as chemical mutagenesis). The use of RNA interference has been met with controversy as off-target effects can make interpreting phenotypic outcomes difficult; however, this has been resolved by creating zebrafish lines that contain stably integrated miRNA constructs that target the desired gene of interest. In this study, we show that a commercially available miRNA vector system with a mouse-derived miRNA backbone is functional in zebrafish and is effective in causing eGFP knockdown in a transient in vivo eGFP sensor assay system. We chose to apply this system to the knockdown of transcripts that are implicated in the human cardiac disorder, Long QT syndrome.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"350352"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/350352","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30573172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Targeting costimulatory molecules to improve antitumor immunity. 靶向共刺激分子提高抗肿瘤免疫。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-02-12 DOI: 10.1155/2012/926321

Daria Capece, Daniela Verzella, Mariafausta Fischietti, Francesca Zazzeroni, Edoardo Alesse

引用次数: 81

Ustekinumab biotherapy and real-time psoriasis capacitance mapping: a pilot study. Ustekinumab生物疗法和实时银屑病电容定位:一项试点研究。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-03-27 DOI: 10.1155/2012/870194

Claudine Piérard-Franchimont, Gérald E Piérard

引用次数: 3

Biological applications of hybrid quantum mechanics/molecular mechanics calculation. 混合量子力学/分子力学计算的生物学应用。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-03-28 DOI: 10.1155/2012/236157

Jiyoung Kang, Yohsuke Hagiwara, Masaru Tateno

{"title":"Biological applications of hybrid quantum mechanics/molecular mechanics calculation.","authors":"Jiyoung Kang, Yohsuke Hagiwara, Masaru Tateno","doi":"10.1155/2012/236157","DOIUrl":"https://doi.org/10.1155/2012/236157","url":null,"abstract":"Since in most cases biological macromolecular systems including solvent water molecules are remarkably large, the computational costs of performing ab initio calculations for the entire structures are prohibitive. Accordingly, QM calculations that are jointed with MM calculations are crucial to evaluate the long-range electrostatic interactions, which significantly affect the electronic structures of biological macromolecules. A UNIX-shell-based interface program connecting the quantum mechanics (QMs) and molecular mechanics (MMs) calculation engines, GAMESS and AMBER, was developed in our lab. The system was applied to a metalloenzyme, azurin, and PU.1-DNA complex; thereby, the significance of the environmental effects on the electronic structures of the site of interest was elucidated. Subsequently, hybrid QM/MM molecular dynamics (MD) simulation using the calculation system was employed for investigation of mechanisms of hydrolysis (editing reaction) in leucyl-tRNA synthetase complexed with the misaminoacylated tRNA(Leu), and a novel mechanism of the enzymatic reaction was revealed. Thus, our interface program can play a critical role as a powerful tool for state-of-the-art sophisticated hybrid ab initio QM/MM MD simulations of large systems, such as biological macromolecules.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"236157"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/236157","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40184139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Comparative study of the effect of baicalin and its natural analogs on neurons with oxygen and glucose deprivation involving innate immune reaction of TLR2/TNFα. 黄芩苷及其天然类似物对TLR2/TNFα先天免疫反应中缺氧、葡萄糖剥夺神经元影响的比较研究。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-03-21 DOI: 10.1155/2012/267890

Hui-Ying Li, Jun Hu, Shuang Zhao, Zhi-Yi Yuan, Hong-Jiao Wan, Fan Lei, Yi Ding, Dong-Ming Xing, Li-Jun Du

引用次数: 44