Journal of Biomedicine and Biotechnology最新文献_第3页

Changes in the material characteristics of maize straw during the pretreatment process of methanation. 玉米秸秆甲烷化预处理过程中材料特性的变化。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-10-14 DOI: 10.1155/2012/325426

Yongzhong Feng, Xiaoling Zhao, Yan Guo, Gaihe Yang, Jianchao Xi, Guangxin Ren

{"title":"Changes in the material characteristics of maize straw during the pretreatment process of methanation.","authors":"Yongzhong Feng, Xiaoling Zhao, Yan Guo, Gaihe Yang, Jianchao Xi, Guangxin Ren","doi":"10.1155/2012/325426","DOIUrl":"https://doi.org/10.1155/2012/325426","url":null,"abstract":"Pretreatment technology is important to the direct methanation of straw. This study used fresh water, four bacterium agents (stem rot agent, \"result\" microbe decomposition agent, straw pretreatment composite bacterium agent, and complex microorganism agent), biogas slurry, and two chemical reagents (sodium hydroxide and urea) as pretreatment promoters. Different treatments were performed, and the changes in the straw pH value, temperature, total solid (TS), volatile solid (VS), and carbon-nitrogen ratio (C/N ratio) under different pretreatment conditions were analyzed. The results showed that chemical promoters were more efficient than biological promoters in straw maturity. Pretreatment using sodium hydroxide induced the highest degree of straw maturity. However, its C/N ratio had to be reduced during fermentation. In contrast, the C/N ratio of the urea-pretreated straw was low and was easy to regulate when used as anaerobic digestion material. The biogas slurry pretreatment was followed by pretreatments using four different bacterium agents, among which the effect of the complex microorganism agent (BA4) was more efficient than the others. The current study is significant to the direct and efficient methanation of straw.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"325426"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/325426","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31019096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by Cupriavidus necator (CCUG52238(T)). 影响Cupriavidus necator (CCUG52238(T))从油棕叶汁中生产聚(3-羟基丁酸酯)的因素

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-10-14 DOI: 10.1155/2012/125865

Mior Ahmad Khushairi Mohd Zahari, Hidayah Ariffin, Mohd Noriznan Mokhtar, Jailani Salihon, Yoshihito Shirai, Mohd Ali Hassan

{"title":"Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by Cupriavidus necator (CCUG52238(T)).","authors":"Mior Ahmad Khushairi Mohd Zahari, Hidayah Ariffin, Mohd Noriznan Mokhtar, Jailani Salihon, Yoshihito Shirai, Mohd Ali Hassan","doi":"10.1155/2012/125865","DOIUrl":"https://doi.org/10.1155/2012/125865","url":null,"abstract":"Factors influencing poly(3-hydroxybutyrate) P(3HB) production by Cupriavidus necator CCUG52238(T) utilizing oil palm frond (OPF) juice were clarified in this study. Effects of initial medium pH, agitation speed, and ammonium sulfate (NH(4))(2)SO(4) concentration on the production of P(3HB) were investigated in shake flasks experiments using OPF juice as the sole carbon source. The highest P(3HB) content was recorded at pH 7.0, agitation speed of 220 rpm, and (NH(4))(2)SO(4) concentration at 0.5 g/L. By culturing the wild-type strain of C. necator under the aforementioned conditions, the cell dry weight (CDW) and P(3HB) content obtained were 9.31 ± 0.13 g/L and 45 ± 1.5 wt.%, respectively. This accounted for 40% increment of P(3HB) content compared to the nonoptimized condition. In the meanwhile, the effect of dissolved oxygen tension (DOT) on P(3HB) production was investigated in a 2-L bioreactor. Highest CDW (11.37 g/L) and P(3HB) content (44 wt.%) were achieved when DOT level was set at 30%. P(3HB) produced from OPF juice had a tensile strength of 40 MPa and elongation at break of 8% demonstrated that P(3HB) produced from renewable and cheap carbon source is comparable to those produced from commercial substrate.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"125865"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/125865","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31033664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Generation of a Chinese hamster ovary cell line producing recombinant human glucocerebrosidase. 产生重组人葡萄糖脑苷酶的中国仓鼠卵巢细胞系的产生。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-10-03 DOI: 10.1155/2012/875383

Juliana Branco Novo, Ligia Morganti, Ana Maria Moro, Adriana Franco Paes Leme, Solange Maria de Toledo Serrano, Isaias Raw, Paulo Lee Ho

{"title":"Generation of a Chinese hamster ovary cell line producing recombinant human glucocerebrosidase.","authors":"Juliana Branco Novo, Ligia Morganti, Ana Maria Moro, Adriana Franco Paes Leme, Solange Maria de Toledo Serrano, Isaias Raw, Paulo Lee Ho","doi":"10.1155/2012/875383","DOIUrl":"https://doi.org/10.1155/2012/875383","url":null,"abstract":"Impaired activity of the lysosomal enzyme glucocerebrosidase (GCR) results in the inherited metabolic disorder known as Gaucher disease. Current treatment consists of enzyme replacement therapy by administration of exogenous GCR. Although effective, it is exceptionally expensive, and patients worldwide have a limited access to this medicine. In Brazil, the public healthcare system provides the drug free of charge for all Gaucher's patients, which reaches the order of $ 84 million per year. However, the production of GCR by public institutions in Brazil would reduce significantly the therapy costs. Here, we describe a robust protocol for the generation of a cell line producing recombinant human GCR. The protein was expressed in CHO-DXB11 (dhfr(-)) cells after stable transfection and gene amplification with methotrexate. As expected, glycosylated GCR was detected by immunoblotting assay both as cell-associated (~64 and 59 kDa) and secreted (63-69 kDa) form. Analysis of subclones allowed the selection of stable CHO cells producing a secreted functional enzyme, with a calculated productivity of 5.14 pg/cell/day for the highest producer. Although being laborious, traditional methods of screening high-producing recombinant cells may represent a valuable alternative to generate expensive biopharmaceuticals in countries with limited resources.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"875383"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/875383","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30995262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Effect of plasminogen activator inhibitor-1 and tissue plasminogen activator polymorphisms on susceptibility to type 2 diabetes in Malaysian subjects. 纤溶酶原激活物抑制剂-1和组织纤溶酶原激活物多态性对马来西亚2型糖尿病易感性的影响

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-04-11 DOI: 10.1155/2012/234937

Zaid Al-Hamodi, Riyadh Saif-Ali, Ikram S Ismail, Khaled A Ahmed, Sekaran Muniandy

{"title":"Effect of plasminogen activator inhibitor-1 and tissue plasminogen activator polymorphisms on susceptibility to type 2 diabetes in Malaysian subjects.","authors":"Zaid Al-Hamodi, Riyadh Saif-Ali, Ikram S Ismail, Khaled A Ahmed, Sekaran Muniandy","doi":"10.1155/2012/234937","DOIUrl":"https://doi.org/10.1155/2012/234937","url":null,"abstract":"Elevated activity of plasminogen activator inhibitor-1 (PAI-1) and decreased tissue plasminogen activator (tPA) activity are considered to be important risk factors for type 2 diabetes mellitus (T2DM) and metabolic syndrome (MetS). The aim of this study was to investigate the association of the PAI-1 4G/5G and tPA Alu-repeat I/D polymorphisms with T2DM in Malaysian subjects. Serum insulin, coronary risk panel, plasma glucose, and PAI-1 4G/5G and tPA Alu-repeat I/D polymorphisms were studied in 303 T2DM subjects (227 with MetS and 76 without MetS) and 131 normal subjects without diabetes and MetS. Statistical analysis showed that the dominant and additive models of PAI-1 4G/5G polymorphism showed a weak association with T2DM without MetS (OR = 2.35, P = 0.045; OR = 1.67, P = 0.058). On the other hand, the recessive model of the tPA Alu-repeat I/D polymorphism showed an association with T2DM with MetS (OR = 3.32, P = 0.013) whereas the dominant and additive models of the tPA Alu-repeat I/D polymorphism were not associated with T2DM either with or without MetS.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"234937"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/234937","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30609869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Applications of the phytomedicine Echinacea purpurea (Purple Coneflower) in infectious diseases. 植物药紫锥菊在感染性疾病中的应用。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2011-10-26 DOI: 10.1155/2012/769896

James B Hudson

{"title":"Applications of the phytomedicine Echinacea purpurea (Purple Coneflower) in infectious diseases.","authors":"James B Hudson","doi":"10.1155/2012/769896","DOIUrl":"https://doi.org/10.1155/2012/769896","url":null,"abstract":"Extracts of Echinacea purpurea (EP, purple coneflower) have been used traditionally in North America for the treatment of various types of infections and wounds, and they have become very popular herbal medicines globally. Recent studies have revealed that certain standardized preparations contain potent and selective antiviral and antimicrobial activities. In addition, they display multiple immune-modulatory activities, comprising stimulation of certain immune functions such as phagocytic activity of macrophages and suppression of the proinflammatory responses of epithelial cells to viruses and bacteria, which are manifested as alterations in secretion of various cytokines and chemokines. These immune modulations result from upregulation or downregulation of the relevant genes and their transcription factors. All these bioactivities can be demonstrated at noncytotoxic concentrations of extract and appear to be due to multiple components rather than the individual chemical compounds that characterize Echinacea extracts. Potential applications of the bioactive extracts may go beyond their traditional uses.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"769896"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/769896","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30293241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 129

Interaction of Helicobacter pylori with C-type lectin dendritic cell-specific ICAM grabbing nonintegrin. 幽门螺杆菌与c型凝集素树突状细胞特异性ICAM抓取非整合素的相互作用。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-04-05 DOI: 10.1155/2012/206463

Eliza Miszczyk, Karolina Rudnicka, Anthony P Moran, Marek Fol, Magdalena Kowalewicz-Kulbat, Magdalena Druszczyńska, Agnieszka Matusiak, Maria Walencka, Wiesława Rudnicka, Magdalena Chmiela

{"title":"Interaction of Helicobacter pylori with C-type lectin dendritic cell-specific ICAM grabbing nonintegrin.","authors":"Eliza Miszczyk, Karolina Rudnicka, Anthony P Moran, Marek Fol, Magdalena Kowalewicz-Kulbat, Magdalena Druszczyńska, Agnieszka Matusiak, Maria Walencka, Wiesława Rudnicka, Magdalena Chmiela","doi":"10.1155/2012/206463","DOIUrl":"https://doi.org/10.1155/2012/206463","url":null,"abstract":"In this study we asked whether Helicobacter pylori whole cells and lipopolysaccharide (LPS) utilize sugar moieties of Lewis (Le) antigenic determinants to interact with DC-SIGN (dendritic cell specific ICAM grabbing nonintegrin) receptor on dendritic cells (DCs). For this purpose the soluble DC-SIGN/Fc adhesion assay and the THP-1 leukemia cells with induced expression of DC-SIGN were used. We showed that the binding specificity of DC-SIGN with H. pylori Le(X/Y) positive whole cells and H. pylori LPS of Le(X/Y) type was fucose dependent, whereas in Le(XY) negative H. pylori strains and LPS preparations without Lewis determinants, this binding was galactose dependent. The binding of soluble synthetic Le(X) and Le(Y) to the DC-SIGN-like receptor on THP-1 cells was also observed. In conclusion, the Le(XY) dependent as well as independent binding of H. pylori whole cells and H. pylori LPS to DC-SIGN was described. Moreover, we demonstrated that THP-1 cells may serve as an in vitro model for the assessment of H. pylori-DC-SIGN interactions mediated by Le(X) and Le(Y) determinants.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"206463"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/206463","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30587433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

Ischemia-induced apoptosis of intestinal epithelial cells correlates with altered integrin distribution and disassembly of F-actin triggered by calcium overload. 缺血诱导的肠上皮细胞凋亡与钙超载引发的整合素分布改变和f -肌动蛋白分解有关。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-05-30 DOI: 10.1155/2012/617539

Zhenyi Jia, Qian Chen, Huanlong Qin

{"title":"Ischemia-induced apoptosis of intestinal epithelial cells correlates with altered integrin distribution and disassembly of F-actin triggered by calcium overload.","authors":"Zhenyi Jia, Qian Chen, Huanlong Qin","doi":"10.1155/2012/617539","DOIUrl":"https://doi.org/10.1155/2012/617539","url":null,"abstract":"The present study examined intestinal epithelial cell (IEC) integrin distribution and disassembly of actin cytoskeleton in response to ischemia-anoxia. Protective effects of calcium channel blocker(CCB) were further examined to explore underlying mechanisms of cellular injury. Materials and Methods. Primary cultures of rat IECs and an in vitro model of ischemia/anoxia were established. IECs were exposed to ischemia/anoxia in the presence and absence of verapamil. The extent of exfoliation was determined using light microscopy while apoptosis rate was measured using flow cytometry. Changes in intracellular calcium, the distribution of integrins and the morphology of F-actin were assessed by confocal microscopy. Results. Detachment and apoptosis of IECs increased following ischemia/anoxia-induced injury. Treatment with verapamil inhibited the detachment and apoptosis. Under control conditions, the strongest fluorescent staining for integrins appeared on the basal surface of IECs while this re-distributed to the apical membrane in response to ischemic injury. Depolymerization of F-actin was also observed in the injured cells. Verapamil attenuated both changes of integrins and F-actin. Conclusions. Redistribution of integrins and disruption of F-actin under ischemia/anoxia injury is associated with IEC detachment and increased apoptosis. These events appeared to be triggered by an increase in Ca(2+)(i) suggesting a potential use for CCB in prevention and treatment of intestinal injury.","PeriodicalId":15089,"journal":{"name":"Journal of Biomedicine and Biotechnology","volume":" ","pages":"617539"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/617539","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30694331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Construction of a llama bacterial artificial chromosome library with approximately 9-fold genome equivalent coverage. 具有9倍基因组等效覆盖率的羊驼细菌人工染色体文库的构建。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-06-28 DOI: 10.1155/2012/371414

K W Airmet, J D Hinckley, L T Tree, M Moss, S Blumell, K Ulicny, A K Gustafson, M Weed, R Theodosis, M Lehnardt, J Genho, M R Stevens, D L Kooyman

引用次数: 3

Security analysis and enhancements of an effective biometric-based remote user authentication scheme using smart cards. 安全分析和增强有效的基于生物特征的智能卡远程用户身份验证方案。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-07-31 DOI: 10.1155/2012/519723

Younghwa An

引用次数: 107

Biometrics and biosecurity. 生物识别和生物安全。

Journal of Biomedicine and Biotechnology Pub Date : 2012-01-01 Epub Date: 2012-09-03 DOI: 10.1155/2012/684815

Tai-Hoon Kim, Sabah Mohammed, Carlos Ramos, Osvaldo Gervasi, Wai-Chi Fang, Adrian Stoica

引用次数: 0